scholarly journals Hepatic mRNA expression of acid labile subunit and deiodinase 1 differs between cows selected for high versus low concentrations of insulin-like growth factor 1 in late pregnancy

2013 ◽  
Vol 96 (6) ◽  
pp. 3737-3749 ◽  
Author(s):  
M. Piechotta ◽  
K. Kedves ◽  
M.Gil Araujo ◽  
A. Hoeflich ◽  
F. Metzger ◽  
...  
Keyword(s):  
Growth Factor ◽  
Mrna Expression ◽  
Late Pregnancy ◽  
Insulin Like Growth Factor ◽  
Low Concentrations ◽  
Acid Labile Subunit ◽  
Acid Labile

scholarly journals Measurement of the acid-labile subunit of the insulin-like growth factor binding protein complex in human serum: a comparison of four immunoassays

2000 ◽  
Vol 165 (2) ◽  
pp. 271-279 ◽  
Author(s):  
RC Baxter ◽  
M Svejkar ◽  
MJ Khosravi ◽  
GL Bennett ◽  
KV Hardman ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Growth Factor ◽  
Protein Complex ◽  
Binding Protein ◽  
Serum Marker ◽  
Healthy Children ◽  
Insulin Like Growth Factor ◽  
Factor Binding ◽  
Acid Labile Subunit ◽  
Acid Labile

The acid-labile subunit (ALS) of the high molecular weight insulin-like growth factor binding protein complex is a liver-derived glycoprotein which is regulated by growth hormone and serves as a serum marker of growth hormone action. We have compared the measurement of ALS by four immunoassay methods (two RIAs, two ELISAs) utilizing various polyclonal and monoclonal antibodies raised against natural or recombinant human ALS, or synthetic ALS peptides. Despite the variety of methodologies and reagents, results obtained by the four methods were highly correlated for 125 sera from various patient groups, and when compared for individual groups of sera from healthy children and adults, growth hormone-deficient children and adults, and subjects with acromegaly. Some weaker correlations among methods were seen when measuring ALS levels in groups of sera from pregnant subjects and subjects with chronic renal failure. An assay using antibodies raised against recombinant ALS yielded lower apparent values than the other methods in patient sera, the discrepancy probably being attributable to a difference in standardization. We conclude that a variety of assay formats and reagents can yield serum ALS values of potential clinical utility.

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