scholarly journals Rumen-protected lysine, methionine, and histidine increase milk protein yield in dairy cows fed a metabolizable protein-deficient diet

2012 ◽  
Vol 95 (10) ◽  
pp. 6042-6056 ◽  
Author(s):  
C. Lee ◽  
A.N. Hristov ◽  
T.W. Cassidy ◽  
K.S. Heyler ◽  
H. Lapierre ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Milk Protein ◽  
Protein Yield ◽  
Deficient Diet ◽  
Metabolizable Protein ◽  
Protein Deficient Diet

scholarly journals Effects of supplemental protein and energy and feeding frequency on the performance of lactating dairy cows fed a protein deficient diet

2002 ◽  
Vol 82 (2) ◽  
pp. 225-231 ◽  
Author(s):  
M. C. Thivierge ◽  
J. F. Bernier ◽  
H. Lapierre
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Protein ◽  
Basal Diet ◽  
Protein Yield ◽  
Meal Frequency ◽  
Feeding Frequency ◽  
Deficient Diet ◽  
Protein Energy ◽  
Protein Deficient Diet

The objective of this experiment was to study the interaction between feeding frequency and supplementation of a protein-deficient diet with an undegradable protein and energy source on milk production and composition. Eight Holstein cows were used in a 2 × 2 factorial arrangement, using a replicated 4 × 4 Latin square design with 14-d experimental periods. The main factors were feeding frequency of the basal diet (two vs. seven times daily) and the addition of a protein-energy supplement fed in seven meals daily. Intake of the basal diet was individually restricted to 95% of previous ad libitum intake. The supplement consisted of 1.71 kg of Pro-Lak® (70% rumen-undegradable protein) and 0.94 kg of dried molasses. The basal diet provided 69, 89, 54, and 95% of the daily requirements of crude protein, rumen-degradable and rumen-undegradable proteins and NEL, respectively. Milk protein yield increased (P = 0.06) by 4% with increased feeding frequency. Milk and milk protein yield both increased (P < 0.01) by 12% with the supplement, but no interaction between feeding frequency and the supplement was observed. Urinary excretion of purine derivatives, an index of rumen microbial protein synthesis, was not affected by treatments. Thus, the results indicate that microbial growth was not affected by meal frequency or the protein-energy supplement, maybe due to the high supply of rumen-degradable protein from the basal diet. The effect of increasing meal frequency on milk protein yield might be related to an increased post-ruminal digestibility of organic matter. Key words: Dairy cows, feeding frequency, milk production, protein, purines

Effect of Metabolisable Protein on intake of grass silage and milk protein yield in dairy cows.

1994 ◽  
Vol 1994 ◽  
pp. 113-113 ◽  
Author(s):  
J.R. Newbold ◽  
B.R. Cottrill ◽  
R.M. Mansbridge ◽  
J.S. Blake
Keyword(s):  
Dairy Cows ◽  
Milk Protein ◽  
Live Weight ◽  
Protein Yield ◽  
Holstein Cows ◽  
Grass Silage ◽  
Lactating Dairy Cows ◽  
Ad Libitum ◽  
Few Data

Few data are available to evaluate the Metabolisable Protein (MP) System (AFRC, 1992) as an aid to ration formulation for dairy cows. Responses of lactating dairy cows to MP were evaluated in two experiments.In Experiment One, groups of 10 Holstein cows were offered grass silage ad libitum and 7kg DM/d of one of six (treatments 1-6) compound foods from 39d after calving, for eight weeks. All rations were isoenergetic, assuming silage intake of 11kg DM/d (typical for the herd). Ratios between MP supply (calculated from in situ protein degradability measurements) and requirement (calculated for a standard cow: 565kg live weight, 281 milk/d, 40g/kg butterfat, 33g/kg milk protein, liveweight change =0kg/d) were 1.00, 1.02, 1.06, 1.06, 1.1 and 1.17 for treatments 1-6, respectively.

scholarly journals Effect of Rumen Available Protein, Amimo Acids and Carbohydrates on Microbial Protein Synthesis, Amino Acid Flow and Performance of High Yielding Cows

1993 ◽  
Author(s):  
Gabriella A. Varga ◽  
Amichai Arieli ◽  
Lawrence D. Muller ◽  
Haim Tagari ◽  
Israel Bruckental ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Dairy Cows ◽  
Milk Protein ◽  
Protein Yield ◽  
Microbial Protein ◽  
Feeding Frequency ◽  
Rumen Undegradable Protein ◽  
And Performance

The effect of rumen available protein amino acids and carbohydrates on microbial protein synthesis, amino acid flow and performance of high yielding dairy cows was studied. A significant relationship between the effective degradabilities of OM in feedstuffs and the in vivo ruminal OM degradation of diets of dairy cows was found. The in situ method enabled the prediction of ruminal nutrients degradability response to processing of energy and nitragenous supplements. The AA profile of the rumen undegradable protein was modified by the processing method. In a continuous culture study total N and postruminal AA flows, and bacterial efficiency, is maximal at rumen degradable levels of 65% of the CP. Responses to rumen degradable non carbohydrate (NSC) were linear up to at least 27% of DM. Higher CP flow in the abomasum was found for cows fed high ruminally degradable OM and low ruminally degradable CP diet. It appeared that in dairy cows diets, the ratio of rumen degradable OM to rumenally degradable CP should be at least 5:1 in order to maximize postruminal CP flow. The efficiency of microbial CP synthesis was higher for diets supplemented with 33% of rumen undegradable protein, with greater amounts of bacterial AA reaching the abomasum. Increase in ruminal carbohydrate availability by using high moisture corn increased proportions of propionate, postruminal nutrients flow, postruminal starch digestibility, ruminal availability of NSC, uptake of energy substrates by the mammory gland. These modifications resulted with improvement in the utilization of nonessential AA for milk protein synthesis, in higher milk protein yield. Higher postruminal NSC digestibility and higher efficiency of milk protein production were recorded in cows fed extruded corn. Increasing feeding frequency increased flow of N from the rumen to the blood, reduced diurnal variation in ruminal and ammonia, and of plasma urea and improved postruminal NSC and CIP digestibility and total tract digestibilities. Milk and constituent yield increased with more frequent feeding. In a study performed in a commercial dairy herd, changes in energy and nitrogenous substrates level suggested that increasing feeding frequency may improve dietary nitrogen utilization and may shift metabolism toward more glucogenesis. It was concluded that efficiency of milk protein yield in high producing cows might be improved by an optimization of ruminal and post-ruminal supplies of energy and nitrogenous substrates. Such an optimization can be achieved by processing of energy and nitrogenous feedstuffs, and by increasing feeding frequency. In situ data may provide means for elucidation of the optimal processing conditions.

scholarly journals Assessment of rumen bacteria in dairy cows with varied milk protein yield

2019 ◽  
Vol 102 (6) ◽  
pp. 5031-5041 ◽  
Author(s):  
M.Y. Xue ◽  
H.Z. Sun ◽  
X.H. Wu ◽  
L.L. Guan ◽  
J.X. Liu
Keyword(s):  
Dairy Cows ◽  
Milk Protein ◽  
Protein Yield ◽  
Rumen Bacteria

Effects of the composition of grass silages on milk production and nitrogen utilization by dairy cows

1996 ◽  
Vol 62 (1) ◽  
pp. 25-34 ◽  
Author(s):  
R. J. Dewhurst ◽  
A. M. Mitton ◽  
N. W. Offer ◽  
C. Thomas
Keyword(s):  
Dairy Cows ◽  
Milk Protein ◽  
Metabolizable Energy ◽  
Protein Yield ◽  
Microbial Protein ◽  
Purine Derivative ◽  
Unit Increase ◽  
Protein Feeding ◽  
Independent Effects ◽  
To Receive

AbstractSixteen varied grass silages metabolizable energy (ME): 9·76 to 11·99 MJ/kg ethanol-corrected toluene dry matter (TDM); crude protein (CP: 149 to 211 g/kg TDM; lactic acid: 3·5 to 134·7 g/kg TDM; butyric acid 0·4 to 46·7 g/kg TDM) were offered ad libitum to early-lactation dairy cows (12 per experiment) along with a fixed allocation of 7 kg/day of a standard concentrate. Four silages were offered in each of four incomplete change-over design experiments with three 21-day periods. This design meant that each cow was allocated to receive three of the four silages evaluated in that experiment. ME intake ranged from 108 to 262 MJ/day (mean 177 (s.d. 30·2)). Similar variation was obtained with milk yields (mean 26·5 (s.d. 4·36) kg/day), fat content (mean 37·7 (s.d. 5·60) g/kg) and protein content (mean 29·0 (s.d. 2·36) g/kg). Urinary purine derivative/creatinine ratio (PD/C), an index of microbial protein measured in spot samples (two per day) averaged 2·92 (s.d. 0·757) mol/mol. Allantoin made up an almost constant molar proportion of PD excretion (mean 0·876 (s.d. 0·0377)), with a small but significant (P < 0·001) decline of 0·0132 (s.d. 0·003) per unit increase in PD/C.Maximal utilization of silage nitrogen occurred with silages having higher ME and lower CP concentrations. Urinary PD/C suggested that microbial protein yield varied in a way which would not be predicted in current schemes and that it was a major source of variation in milk protein yield under the conditions of the present experiment. Principal components regression confirmed independent effects of ME supply and MP supply (indexed by urinary PD/C) on milk protein yield. Further work should pursue the possibility of using the urinary PD/C technique to refine protein feeding at the farm level.

Effect of duodenal proline infusion on milk production and composition in dairy cows

1991 ◽  
Vol 53 (3) ◽  
pp. 299-303 ◽  
Author(s):  
I. Bruckental ◽  
I. Ascarelli ◽  
B. Yosif ◽  
E. Alumot
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Milk Fat ◽  
Protein Concentration ◽  
Milk Protein ◽  
Protein Yield ◽  
Energy Utilization ◽  
Dietary Energy ◽  
Arginine Uptake ◽  
Friesian Cows

AbstractFour Israeli-Friesian cows, two at peak and two at mid lactation, were fitted with duodenal cannulas. They were given proline or water infusions for 8-day periods to evaluate the effect of duodenal infusion of proline on arginine uptake by the mammary gland and on milk yield and composition of dairy cows. Milk fat content and 40 g/kg fat-corrected milk yield increased for all cows due to proline infusion. Milk protein concentration during either early or mid lactation and milk protein yield during early lactation, were not affected by proline infusion. Milk protein yield during mid lactation was increased due to proline infusion. Arginine uptake by the udder dropped by one-half during proline infusion. Results suggest that post-ruminal administration of supplemental proline reduced requirements for arginine by the udder and improved efficiency of dietary energy utilization.

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