scholarly journals Short communication: Evaluation of confirmatory stains used for direct microscopic somatic cell counting of sheep milk

2011 ◽  
Vol 94 (4) ◽  
pp. 1908-1912 ◽  
Author(s):  
K.H. Petersson ◽  
L.A. Connor ◽  
C.S. Petersson-Wolfe ◽  
K.A. Rego
2009 ◽  
Vol 92 (3) ◽  
pp. 990-995 ◽  
Author(s):  
A.C.O. Rodrigues ◽  
L.D. Cassoli ◽  
P.F. Machado ◽  
P.L. Ruegg

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 268
Author(s):  
Daphne T. Lianou ◽  
Charalambia K. Michael ◽  
Natalia G. C. Vasileiou ◽  
Efthymia Petinaki ◽  
Peter J. Cripps ◽  
...  

Objectives were to investigate somatic cell counts (SCC) and total bacterial counts (TBC) in the raw bulk-tank milk of sheep flocks in Greece, to study factors potentially influencing increased SCC and TBC in the bulk-tank milk of sheep and to evaluate possible associations of SCC and TBC with milk content. Throughout Greece, 325 dairy sheep flocks were visited for collection of milk sampling for somatic cell counting, microbiological examination and composition measurement. Geometric mean SCC were 0.488 × 106 cells mL−1; geometric mean TBC were 398 × 103 cfu mL−1; 228 staphylococcal isolates were recovered form 206 flocks (63.4%). Multivariable analyses revealed annual incidence risk of clinical mastitis, age of the farmer and month into lactation period (among 53 variables) to be significant for SCC > 1.0 × 106 cells mL−1 and month into lactation period at sampling and availability of mechanical ventilators (among 58 variables) to be significant for TBC > 1500 × 103 cfu mL−1. Negative correlation of SCC with fat, total protein and lactose and positive correlation of SCC with added water were found. With SCC > 1.0 × 106 cells mL−1, significant reduction of protein content (2%) was observed, whilst in flocks with SCC > 1.5 × 106 cells mL−1, significantly lower annual milk production per ewe (42.9%) was recorded.


2020 ◽  
Vol 103 (2) ◽  
pp. 1269-1275 ◽  
Author(s):  
Marie R. Lawton ◽  
Katharine G. Jencarelli ◽  
Sarah M. Kozak ◽  
Samuel D. Alcaine

2020 ◽  
Vol 103 (3) ◽  
pp. 2701-2706
Author(s):  
Rajnish K. Yadav ◽  
Manjari Singh ◽  
Subhadeep Roy ◽  
Swetlana Gautam ◽  
Jitendra K. Rawat ◽  
...  

1973 ◽  
Vol 56 (4) ◽  
pp. 950-956
Author(s):  
Wesley N Kelley

Abstract A collaborative study was conducted to compare tbe automated optical somatic cell counting method (OSCC) with the direct microscopic somatic cell counting method (DMSCC) for raw milk. Samples were prefixed with formaldehyde and introduced into an Auto-Analyzer system. Dilution, clarification, and cell counting were performed automatically. Eight collaborators participated in the study, analyzing 48 samples in duplicate, using 2 different sampling rates. The results were compared with DMSCC counts reported by 3 different analysts. Statistical results show that the standard deviation for the DMSCC method was 0.1086 and for the OSCC method, at a sampling rate of 30/hr, 0.0911. From comparison of results it appears that the OSCC method is as accurate as, and more precise than, the DMSCC method. The faster sampling rate of the OSCC method (60/hr) has some effect on precision but little effect on accuracy. The method has been adopted as official first action.


2004 ◽  
Vol 3 (2) ◽  
pp. 147-156 ◽  
Author(s):  
Leonardo Bianchi ◽  
Carmen Casoli ◽  
Mariano Pauselli ◽  
Elena Budelli ◽  
Anna Caroli ◽  
...  

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