scholarly journals In Vitro Assessment of the Gastrointestinal Transit Tolerance of Taxonomic Reference Strains from Human Origin and Probiotic Product Isolates of Bifidobacterium

2007 ◽  
Vol 90 (8) ◽  
pp. 3572-3578 ◽  
Author(s):  
L. Masco ◽  
C. Crockaert ◽  
K. Van Hoorde ◽  
J. Swings ◽  
G. Huys
Keyword(s):  
Gastrointestinal Transit ◽  
Human Origin ◽  
Probiotic Product ◽  
In Vitro Assessment ◽  
Reference Strains ◽  
Transit Tolerance

scholarly journals In vitro assessment of metabolic profile of Enterococcus strains of human origin

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Ashlesha Bhagwat ◽  
Uday S. Annapure
Keyword(s):  
Metabolic Profile ◽  
Food Industry ◽  
Radical Scavenging ◽  
Starter Cultures ◽  
Bile Salt Hydrolase ◽  
Human Origin ◽  
Future Potential ◽  
In Vitro Assessment ◽  
Radical Scavenging Properties

Abstract Background In the present study, previously isolated, safe, and avirulent enterococci strains were exploited for their metabolic profile (Bhagwat et al., Asian J Pharm Clin Res 12: 2019). Results Thirteen enterococci strains of human origin produced important enzymes like amylase (0.5–0.7 mg ml−1), protease (192–264 mg ml−1), lipase (8–10 mg ml−1), bile salt hydrolase, conjugated linoleic acid (CLA), and lactic acid (highest 12 mg ml−1), thus implicating potential attributes of starter cultures in food and dairy industry. Biogenic amines like arginine and tryptamine were produced after 4 days above 25 °C. Castor oil (highest yield 60 μg ml−1) and sunflower oil (highest yield 48 μg ml−1) both proved to be excellent sources of CLA production. Reduction assays using FRAP, ABTS (above 83%), and DPPH (30–50%) revealed excellent radical scavenging properties of cell-free supernatants of Enterococcus strains. Conclusion The results implicate the future potential of application enterococci for therapeutic purpose as well as the food industry.

In Vitro Assessment of Actinobacteria for Survivability Under Simulated Gastrointestinal Transit

2022 ◽  
pp. 545-548
Author(s):  
Vinothini Gopal ◽  
M. S. Shabeena Banu ◽  
Seema Siddharthan ◽  
Dhanasekaran Dharumadurai
Keyword(s):  
Gastrointestinal Transit ◽  
In Vitro Assessment

scholarly journals IN VITRO ASSESSMENT OF PHYSIOLOGICAL PROPERTIES OF ENTEROCOCCUS STRAINS OF HUMAN ORIGIN FOR POSSIBLE PROBIOTIC USE

2019 ◽  
pp. 194-203
Author(s):  
ASHLESHA BHAGWAT ◽  
NANDANWAR Y S ◽  
RAHUL WARKE ◽  
UDAY S ANNAPURE
Keyword(s):  
Animal Feed ◽  
Probiotic Properties ◽  
Human Origin ◽  
Entamoeba Dispar ◽  
Urogenital Infections ◽  
Physiological Properties ◽  
Wide Range ◽  
Human Vagina ◽  
In Vitro Assessment

Objective: The human vagina and gut are known to harbor a wide range of bacteria. Some useful bacteria maintain the vaginal pH around 3–4.5. This acidity of the vagina helps to inhibit pathogenic organisms that cause urogenital infections. The present study involved screening of previously identified Enterococcus strains of human origin for their probiotic physiological properties. Materials and Methods: The strains were screened for their biofilm, antibiofilm, antagonistic, antibiotic resistance, adherence, aggregating, and hydrogen peroxide production abilities. Results: Enterococcus canintestini S26B, Entamoeba dispar S27A, E. dispar S26A, E. dispar S20B, E. canintestini AB2, Enterococcus villorum SB2, and Enterococcus rivorum S22C displayed in vitro probiotic properties. Conclusions: These strains can be used as probiotic candidates and may prove their potential in human or animal feed only after further clinical studies.

In vitro assessment of Labisia pumila and its constituents for interactions with CYPs, P-GP and PXR

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
VK Manda ◽  
OR Dale ◽  
C Awortwe ◽  
Z Ali ◽  
IA Khan ◽  
...  
Keyword(s):  
In Vitro Assessment ◽  
Labisia Pumila

In Vitro Assessment of Hydroxyapatite Coating on the Surface of Additive Manufactured Ti6Al4V Scaffolds

2016 ◽  
Vol 879 ◽  
pp. 2444-2449 ◽  
Author(s):  
Ekaterina Chudinova ◽  
Maria Surmeneva ◽  
Andrey Koptioug ◽  
Irina V. Savintseva ◽  
Irina Selezneva ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Nanocrystalline Structure ◽  
X Ray Diffraction ◽  
Ha Coating ◽  
Magnetron Sputter Deposition ◽  
In Vitro Assessment ◽  
Average Size

Custom orthopedic and dental implants may be fabricated by additive manufacturing (AM), for example using electron beam melting technology. This study is focused on the modification of the surface of Ti6Al4V alloy coin-like scaffolds fabricated via AM technology (EBM®) by radio frequency (RF) magnetron sputter deposition of hydroxyapatite (HA) coating. The scaffolds with HA coating were characterized by Scanning Electron microscopy, X-ray diffraction. HA coating showed a nanocrystalline structure with the crystallites of an average size of 32±9 nm. The ability of the surface to support adhesion and the proliferation of human mesenchymal stem cells was studied using biological short-term tests in vitro. In according to in vitro assessment, thin HA coating stimulated the attachment and proliferation of cells. Human mesenchymal stem cells cultured on the HA-coated scaffold also formed mineralized nodules.

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