scholarly journals In-vitro antidiabetic activities, chemical compositions, antioxidant activities, and toxicity of black tea polysaccharides as a potential source of dietary ingredients

2021 ◽  
Vol 13 ◽  
Author(s):  
Ebru Pelvan ◽  
Ayse Karadag ◽  
Kubra Dogan ◽  
Soner Aksu ◽  
Arzu Tas ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Glucose Uptake ◽  
Antioxidant Activities ◽  
Lethal Dose ◽  
Black Tea ◽  
Chemical Compositions ◽  
Low Grade ◽  
Potential Health ◽  
Tea Polysaccharides

Type 2 diabetes (T2D) is one of the fast growing diet-related chronic diseases throughout the world. Tea contains several bioactive compounds, some of which render health benefits. Black tea polysaccharides (BTPS), extracted from low grade quality tea leaves, after processing, were examined for their in-vitro antidiabetic activities (α-glucosidase inhibitory and glucose uptake activities), chemical compositions (yield, monosaccharides, amino acids, and minerals), and antioxidant activities as well as toxicity (cytotoxicity and genotoxicity). In addition, 50% lethal dose (LD50) for BTPS was determined using an acute toxicity test to assess the safe use of it as a dietary ingredient. BTPS had strong α-glucosidase inhibitory activity with IC50 value of 3.4 µg/mL. This was much lower than that of the positive control, pharmaceutical glucosidase inhibitor acarbose with IC50 of 1.06 mM. BTPS also increased glucose uptake into the adipocyte differentiated 3T3-L1 MBX cells. Neither cytotoxic nor mutagenic effects were found for BTPS. The LD50 of BTPS for acute toxicity demonstrated that it was safe to use. The present work suggests that BTPS can be used as an antidiabetic dietary ingredient without posing any potential health risk.

scholarly journals α-Glucosidase inhibitory activities, functional properties, and safety of green tea polysaccharides as a potential source of dietary supplement

2018 ◽  
Vol 3 ◽  
pp. 124-132
Author(s):  
Ayse Karadag ◽  
Kubra Dogan ◽  
Ebru Pelvan ◽  
Arzu Tas ◽  
Ozlem Atli Eklioglu ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Dietary Supplement ◽  
Green Tea ◽  
Lethal Dose ◽  
Monosaccharide Composition ◽  
Total Phenolic ◽  
Potential Health ◽  
Tea Polysaccharides ◽  
Mutagenic Effects

Procuring valuable bioactive compounds from low quality green tea is of great interest. Green tea polysaccharides (GTPS), obtained from low quality tea leaves, were examined for their yield, monosaccharide composition, total phenolic content, antioxidant potential, and structures as well as molecular weight distribution and in vitro anti-diabetic activities. GTPS were also evaluated for their toxicity by performing cytotoxicity and genotoxicity. Additionally, 50% lethal dose (LD50) for GTPS was determined using an acute toxicity test to assess the safe use of it as a dietary supplement. Monosaccharides of GTPS were mainly composed of xylose, glucose, ribose, galactose, arabinose, and glucuronic acid. GTPS exhibited 91.86% α-glucosidase inhibition at 0.5 mg/mL concentration. The α-glucosidase inhibitory activity of GTPS was higher than that of pharmacological glucosidase inhibitor, acarbose, which exhibited only 47.86% inhibition at 2.5 mg/mL concentration. Neither cytotoxic nor no mutagenic effects were found for GTPS. The acute toxicity also showed that LD50 of GTPS was greater than 5,000 mg/kg. The present work suggests that GTPS can be considered as an anti-diabetic dietary supplement without posing any potential health risk.

scholarly journals Correction: In-vitro antidiabetic activities, chemical compositions, antioxidant activities, and toxicity of black tea polysaccharides as a potential source of dietary ingredients

2021 ◽  
Vol 14 ◽  
pp. 126-127
Author(s):  
Ebru Pelvan ◽  
Ayse Karadag ◽  
Kubra Dogan ◽  
Soner Aksu ◽  
Soner Aksu ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Black Tea ◽  
Chemical Compositions ◽  
Potential Source ◽  
Corrected Version ◽  
Tea Polysaccharides

There is an error in Table 2 in the published article, Journal of Food Bioactives, 2021;13:93–101, doi: 10.31665/JFB.2021.13263. The correct value in column “mg/100 g” under “D-glucose” is “59.50 ± 7.80”, not “9.50 ± 7.80”. The authors have included a corrected version of the table below.

scholarly journals Antioxidant Activity and Main Chemical Components of a Novel Fermented Tea

Molecules ◽  
2019 ◽  
Vol 24 (16) ◽  
pp. 2917 ◽  
Author(s):  
Tao Tong ◽  
Ya-Juan Liu ◽  
Jinhong Kang ◽  
Cheng-Mei Zhang ◽  
Seong-Gook Kang
Keyword(s):  
Antioxidant Activities ◽  
Reducing Power ◽  
Black Tea ◽  
Chemical Components ◽  
Total Phenolic ◽  
Food Ingredient ◽  
Functional Activities ◽  
Overall Acceptability ◽  
High Level

In the present study, we aimed to develop a novel fermented tea (NFT) product and to evaluate their in vitro antioxidant potential and chemical composition. We found that NFT contained a high level of total phenolic compounds (102.98 mg gallic acid equivalents/g extract) and exhibited diverse antioxidant activities, such as scavenging of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and hydroxyl radicals, as well as reducing power. The total catechins in NFT were comparable to those of Lipton black tea (LBT), but lower than those of Boseong green tea (BGT) or Tieguanyin oolong tea (TOT). Among all catechins tested, epigallocatechin (EGC) and epigallocatechin-3-O-gallate (EGCG) were the predominant compounds in NFT. In particular, the contents of total theaflavins (TFs), theaflavin (TF), theaflavin-3-gallate (TF3G), and theaflavin-3’-gallate (TF3’G) in NFT were significantly higher than that of BGT, TOT, or LBT. NFT had the highest level of total essential amino acid and γ-aminobutyric acid (GABA) compared with BGT, TOT and LBT. Furthermore, the sensory evaluation results showed that NFT had satisfactory color, aroma, taste, and overall acceptability scores. Our results highlight the potential usefulness of this novel fermented tea as a nutraceutical food/ingredient with special functional activities.

In vitro anti-hyperglycemic, antioxidant activities and intestinal glucose uptake evaluation of Endiandra kingiana extracts

2020 ◽  
Vol 25 ◽  
pp. 101594
Author(s):  
Mohamad Hafizi Abu Bakar ◽  
Pui Yee Lee ◽  
Mohamad Nurul Azmi ◽  
Nurul Syifa’ Lotfiamir ◽  
Mohamad Shamil Faris Mohamad ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Antioxidant Activities

scholarly journals Phenolic Acids of Plant Origin—A Review on Their Antioxidant Activity In Vitro (O/W Emulsion Systems) Along with Their in Vivo Health Biochemical Properties

Foods ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 534 ◽  
Author(s):  
Sotirios Kiokias ◽  
Charalampos Proestos ◽  
Vassiliki Oreopoulou
Keyword(s):  
Phenolic Acids ◽  
Antioxidant Activities ◽  
Biochemical Properties ◽  
Potential Health ◽  
Wide Range ◽  
Literature Evidence ◽  
Emulsion Systems ◽  
Review Reports

Nature has generously offered a wide range of herbs (e.g., thyme, oregano, rosemary, sage, mint, basil) rich in many polyphenols and other phenolic compounds with strong antioxidant and biochemical properties. This paper focuses on several natural occurring phenolic acids (caffeic, carnosic, ferulic, gallic, p-coumaric, rosmarinic, vanillic) and first gives an overview of their most common natural plant sources. A summary of the recently reported antioxidant activities of the phenolic acids in o/w emulsions is also provided as an in vitro lipid-based model system. Exploring the interfacial activity of phenolic acids could help to further elucidate their potential health properties against oxidative stress conditions of biological membranes (such as lipoproteins). Finally, this review reports on the latest literature evidence concerning specific biochemical properties of the examined phenolic acids.

scholarly journals Antimicrobial, antioxidant activities and toxicity on Cavia porcellus of Dialium angolense Welw. Ex Oliv, a traditional medicinal plant from Bagira in Eastern of DR Congo

2020 ◽  
Vol 13 (2) ◽  
pp. 166-180
Author(s):  
Bashige Chiribagula V ◽  
Bakari Amuri S ◽  
Okusa Ndjolo Philippe ◽  
Kahumba Byanga J ◽  
Duez P ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Acute Toxicity ◽  
Antioxidant Activities ◽  
Antimicrobial Properties ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Cavia Porcellus ◽  
E Coli

Dialium angolense is used in Bagira for its various medicinal properties particularly in the management of infectious diseases. In this study, the methanol and aqueous extracts of leaves and fruits were evaluated for their in vitro antioxidant and antimicrobial properties and their in vivo toxicity on Cavia porcellus. The major phytochemical classes of extracts were screened using standard in-tube reactions. The antimicrobial study was tested on Candida albicans, Escherichia coli, Salmonella typhi, Staphylococcus aureus and Streptococcus pneumoniae using agar well diffusion and dilution methods, while the antioxidant activity was evaluated by a DPPH assay. For the acute toxicity study, animals (6/group) were orally given in a single dose 5000, 1000 or 15000 mg of extract/kg body weight (BW) then observed for 14 days. In sub-acute toxicity assays, 150 or 300 mg/kg BW/day were orally given, and animals observed for 28 days. Total phenolics and total flavonoids contents ranged 1.19 to 1.61 mg GAE.g-1 and 0.45 to 1.01 mg QEg-1, respectively. The extracts presented antioxidant activity with IC50 ranging 4.9 to 6.9 µg/mL. The minimal inhibitory concentration (MIC) on tested strains ranged from 1.9 to 500 µg/mL with the aqueous extract of fruits as a most active extract: MIC=1.9 µg/mL on E. coli and C. albicans. No signs of toxicity were noted during the acute and sub-acute toxicity assessments, suggesting a maximal tolerate doses (MDT) and LD50 > 15000 mg/kg BW. This study highlights the antioxidant and antimicrobial activities of Dialium angolense and suggests that further studies be directed towards the isolation of active compounds.

scholarly journals In vitro gastrointestinal digestion of a bisdemethoxycurcumin-rich Curcuma longa extract and its oral bioavailability in rats

2021 ◽  
Vol 45 (1) ◽  
Author(s):  
Venkataramana Heggar Sudeep ◽  
Kuluvar Gouthamchandra ◽  
Siddappa Chandrappa ◽  
Puttaswamy Naveen ◽  
Budanuru Reethi ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Curcuma Longa ◽  
Radical Scavenging ◽  
Relative Bioavailability ◽  
Inhibitory Effect ◽  
Gastrointestinal Digestion ◽  
Potential Health ◽  
Turmeric Extract ◽  
In Vitro Gastrointestinal Digestion

Abstract Background Nonetheless curcumin has potential health benefits, its low bioavailability limits the application of conventional turmeric extract with curcumin as major curcuminoid. This is a comparative study to assess the stability, bioaccessibility and biological activity of BDMC in standardized C. longa extract (REVERC3) relative to curcumin in regular turmeric extract (RTE). Here we report the preparation of a standardized Curcuma longa extract (REVERC3™) standardized to contain 75 ± 5 w/w % bisdemethoxycurcumin (BDMC), 1.2 ± 0.8 w/w % curcumin and 10 ± 5 w/w % demethoxycurcumin (DMC). The turmeric extracts were subjected to in vitro gastrointestinal digestion and the curcuminoids in undigested and digested samples were analyzed using HPLC to determine the bioaccessibility. Further, the undigested and digested samples were evaluated for lipase inhibition and antioxidant activities. Male Wistar rats were administered with single dose (1000 mg/kg) of standardized C. longa extract and RTE to determine the plasma concentration of BDMC and curcumin respectively at different time points using LCMS/MS. Results The bioaccessibility of BDMC was significantly higher than curcumin (p < 0.05). BDMC was found superior to curcumin having significant lipase inhibitory effect (p < 0.01), ABTS radical scavenging (p < 0.05), and nitric oxide scavenging activities (p < 0.01). Interestingly, the relative bioavailability of BDMC in standardized C. longa extract was 18.76 compared to curcumin. The Cmax of BDMC was 4.4-fold higher than curcumin. Conclusion BDMC is reported to have higher bioaccessibility and bioavailability than curcumin. Our findings rationalize use of BDMC-enriched standardized C. longa extract for improved physiological benefits counteracting the regular turmeric extract with less bioavailable curcumin as major curcuminoid.

scholarly journals Chemical compositon, antibacterial and antioxidant activities of Cnidium silaifolium ssp. orientale (Boiss.) Tutin essential oils

2021 ◽  
Vol 72 (2) ◽  
pp. e403
Author(s):  
A.E. Karadağ ◽  
B. Demirci ◽  
Ö. Çeçen ◽  
F. Tosun ◽  
F. Demirci
Keyword(s):  
Essential Oils ◽  
Antioxidant Activities ◽  
Chemical Compositions ◽  
Significant Activity ◽  
Gram Positive Bacteria ◽  
Microdilution Method ◽  
Aerial Parts ◽  
Germacrene D ◽  
Different Parts

The chemical compositions of the essential oils (EOs) obtained by hydrodistillation from different parts of Cnidium silaifolium ssp. orientale (Boiss.) Tutin were analyzed both by GC-FID and GC/MS, simultaneously. One hundred nine compounds representing 90.1% of the total volatiles in the EOs were identified with the main characteristic compounds α-pinene (50.3%) in the root, germacrene D (20.3%) in the fruit, and β-caryophyllene (18.7%) in the aerial parts of C. silaifolium ssp. orientale. The antimicrobial activity against human pathogenic Gram-negative and Gram-positive bacteria was evaluated by the in vitro microdilution method. Antibacterial succeptibility was observed from the root and aerial part EOs against Staphylococcus aureus (0.039 and 0.156 mg/mL, respectively); while the fruit EO was most effective against Bacillus cereus at 0.07 mg/mL. The antioxidant capacities of the EOs were also evaluated by in vitro DPPH• and ABTS•+ scavenging assays, where no significant activity was observed compared to ascorbic acid and Trolox.

scholarly journals Phytopharmacological Evaluation of Alcoholic Extract of Berberis aristata Leaf in the Treatment of Gastric Ulcer

2020 ◽  
Vol 13 (1) ◽  
pp. 1-5
Author(s):  
Ratnaker Singh ◽  
Y. Trilochana
Keyword(s):  
Acute Toxicity ◽  
Crude Extract ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Antiulcer Activity ◽  
Alcoholic Extract ◽  
Dose Dependent

For over a century, peptic ulcer has been one of the most common gastrointestinal tract (GIT) disorder. There are number of drugs are now available for treatment. Drugs of herbal origin reduce the offensive factors and have proved to be safe, clinically effective, relatively less expensive, globally competitive, and with better patient tolerance.This study was performed to assess the anti-ulcer activity on different parts of B.aristata. Apart from that, acute toxicity, qualitative chemical analysis, total phenolic content (TPC), total flavonoid content(TFC) and in vitro antioxidant activities were evaluated. The potentially active plant part was selected for screening as gastro protective, in vivo antioxidant and antisecretory activities in ulcerated rats.The 50% ethanolic extract of B. aristata were subjected to preliminary phytochemical screening, estimation of TFC and TPC. The crude extract from the leaves of B. aristata gave best antiulcer activity among flower and stem. In acute toxicity studies, the administration of the crude extract of B. aristata leaves did not reveal any adverse effects or toxicity in rats at fourteen days observations.The results of these studies have shown that ethylexract of B.aristata leaf (EEBAL) produced a significant dose dependent ulcerprotective, antioxidant and antisecretory activity by blocking the activity of proton pump, protecting from antioxidants produced during stress induced ulcer and by enhancing glycoprotein levels.

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