Development of SSR Markers Based on Transcriptome Sequences of the Wolf Spider Pardosa pseudoannulata (Araneae: Lycosidae)1

2020 ◽  
Vol 129 (1) ◽  
pp. 6
Author(s):  
Chang-chun Li ◽  
Guo-yuan Li ◽  
Yong Wang ◽  
Yu Peng
Chemosphere ◽  
2021 ◽  
Vol 268 ◽  
pp. 129239
Author(s):  
Juan Wang ◽  
Yuan He ◽  
Xianjin Peng ◽  
Bo lv ◽  
Zhi Wang ◽  
...  

Chemosphere ◽  
2020 ◽  
Vol 248 ◽  
pp. 125904 ◽  
Author(s):  
Juan Wang ◽  
Xianjin Peng ◽  
Huilin Yang ◽  
Bo Lv ◽  
Zhi Wang ◽  
...  

2019 ◽  
Author(s):  
Na Yu ◽  
Jingjing Li ◽  
Meng Liu ◽  
Lixin Huang ◽  
Haibo Bao ◽  
...  

AbstractSpiders constitute an extensive and diverse branch of the phylum Arthropoda. Whereas the genomes of four web-weaver spider species and a single cave-living spider have been determined, similar studies have not been reported previously for a wandering spider. The pond wolf spider, Pardosa pseudoannulata, is a wandering hunter that immobilizes prey using venom rather than a web. It is also an important predator against a range of agriculturally important insect pests. The increasing interest in its wandering lifestyle and in the potential of spider venom as a tool for pest control have prompted a detailed study on this wandering spider species. We have generated a high-quality genome sequence of P. pseudoannulata and analysed genes associated with the production of silk and venom toxins. Sequencing reveals that P. pseudoannulata has a large genome of 4.26 Gb. The presence of only 16 spidroin genes and four types of silk glands is consistent with the moderate use of silk and the lack of a prey-catching web. A large number of genes encode neurotoxins and there is evidence that the majority are highly selective for invertebrates. Comparison between spider species reveals a correlation between spider neurotoxin selectivity for target organisms and spider prosoma size, suggesting a possible coevolution of these two features. The genome data provides valuable insights into the biology of P. pseudoannulata and its potential role as a natural enemy in pest control.


2016 ◽  
Vol 17 (12) ◽  
pp. 2033 ◽  
Author(s):  
Chang-Chun Li ◽  
Yong Wang ◽  
Guo-Yuan Li ◽  
Yue-Li Yun ◽  
Yu-Jun Dai ◽  
...  

2018 ◽  
Vol 17 (03) ◽  
pp. 280-284 ◽  
Author(s):  
Katina F. Olodo ◽  
Mame C. Gueye ◽  
Caroline Calatayud ◽  
Baye M. Diop ◽  
Ndjido A. Kane ◽  
...  

AbstractDigitaria exilis is an important indigenous cereal in West Africa. The first fonio reference transcriptome was released and became a key tool for developing new molecular markers contributing to a better understanding of its genetic diversity. A total of 126 new putative primer pairs were successfully designed in 37,327 unigenes from the D. exilis transcriptome. Thirty-seven primer pairs were randomly selected and tested for their ability to cross-amplify to related species. Clear amplification patterns were observed on 24 primer pairs. Of these, 71, 74 and 35% showed polymorphism in three species: D. exilis, D. longiflora and D. iburua. The transferability from D. exilis was 96% to D. longiflora and 71% to D. iburua. The new SSR markers confirmed the close genetic proximity of D. exilis with D. longiflora and its stronger genetic difference of D. exilis from D. iburua. These markers will be valuable for completing future knowledge on Digitaria evolutionary history, and for testing gene flows between related species.


PLoS ONE ◽  
2016 ◽  
Vol 11 (6) ◽  
pp. e0158011 ◽  
Author(s):  
Xiangkun Meng ◽  
Chunrui Li ◽  
Chunli Xiu ◽  
Jianhua Zhang ◽  
Jingjing Li ◽  
...  

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