scholarly journals TRANSFORMATION OF BORIC ACID WITH HYDROXYL-CONTAINING COMPOUNDS IN THE SOLID-PHASE SYSTEM

2018 ◽  
Vol 10 (3-4) ◽  
Author(s):  
S. Prymachenko ◽  
◽  
V. Maksin ◽  
Keyword(s):  
Boric Acid ◽  
Solid Phase ◽  
Phase System

Influence of biomass on hydrodynamics of an internal loop airlift reactor

2006 ◽  
Vol 60 (6) ◽  
Author(s):  
M. Juraščík ◽  
M. Hucík ◽  
I. Sikula ◽  
J. Annus ◽  
J. Markoš
Keyword(s):  
Aspergillus Niger ◽  
Gluconic Acid ◽  
Solid Phase ◽  
Biomass Concentration ◽  
Airlift Reactor ◽  
Phase System ◽  
Two Phase ◽  
Experimental Conditions ◽  
Internal Loop ◽  
Three Phase

AbstractThe effect of the biomass presence on the overall circulation velocity, the linear velocities both in the riser and the downcomer and the overall gas hold-up was studied in a three-phase internal loop airlift reactor (ILALR). The measured data were compared with those obtained using a two-phase system (air—water). All experiments were carried out in a 40 dm3 ILALR at six different biomass concentrations (ranging from 0 g dm−3 to 7.5 g dm−3), at a temperature of 30°C, under atmospheric pressure. Air and water were used as the gas and liquid model media, respectively. Pellets of Aspergillus niger produced during the fermentation of glucose to gluconic acid in the ILALR were considered solid phase. In addition, liquid velocities were measured during the fermentation of glucose to gluconic acid using Aspergillus niger. All measurements were performed in a bubble circulation regime. At given experimental conditions the effect of the biomass on the circulation velocities in the ILALR was negligible. However, increasing of the biomass concentration led to lower values of the total gas hold-up.

scholarly journals Application of Hollow Fibre-Liquid Phase Microextraction Technique for Isolation and Pre-Concentration of Pharmaceuticals in Water

Membranes ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 311
Author(s):  
Lawrence Mzukisi Madikizela ◽  
Vusumzi Emmanuel Pakade ◽  
Somandla Ncube ◽  
Hlanganani Tutu ◽  
Luke Chimuka
Keyword(s):  
Liquid Phase ◽  
Solid Phase ◽  
Deep Eutectic Solvents ◽  
Enrichment Factors ◽  
Hollow Fibre ◽  
Phase System ◽  
Phase Extraction ◽  
Liquid Phase Microextraction ◽  
Three Phase ◽  
Set Up

In this article, a comprehensive review of applications of the hollow fibre-liquid phase microextraction (HF-LPME) for the isolation and pre-concentration of pharmaceuticals in water samples is presented. HF-LPME is simple, affordable, selective, and sensitive with high enrichment factors of up to 27,000-fold reported for pharmaceutical analysis. Both configurations (two- and three-phase extraction systems) of HF-LPME have been applied in the extraction of pharmaceuticals from water, with the three-phase system being more prominent. When compared to most common sample preparation techniques such as solid phase extraction, HF-LPME is a greener analytical chemistry process due to reduced solvent consumption, miniaturization, and the ability to automate. However, the automation comes at an added cost related to instrumental set-up, but a reduced cost is associated with lower reagent consumption as well as shortened overall workload and time. Currently, many researchers are investigating ionic liquids and deep eutectic solvents as environmentally friendly chemicals that could lead to full classification of HF-LPME as a green analytical procedure.

scholarly journals Detection of the Ovarian Cancer Biomarker Lysophosphatidic Acid in Serum

Biosensors ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 13 ◽  
Author(s):  
Brian De La Franier ◽  
Michael Thompson
Keyword(s):  
Ovarian Cancer ◽  
Lysophosphatidic Acid ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Medical Condition ◽  
Large Population ◽  
Rapid Screening ◽  
Phase System ◽  
Serum Samples ◽  
Early Results

Lysophosphatidic acid (LPA) is present during the medical condition of ovarian cancer at all stages of the disease, and, therefore possesses considerable potential as a biomarker for screening its presence in female patients. Unfortunately, there is currently no clinically employable assay for this biomarker. In the present work, we introduce a test based on the duel protein system of actin and gelsolin that could allow the quantitative measurement of LPA in serum samples in a biosensing format. In order to evaluate this possibility, actin protein was dye-modified and complexed with gelsolin protein, followed by surface deposition onto silica nanoparticles. This solid-phase system was exposed to serum samples containing various concentrations of LPA and analyzed by fluorescence microscopy. Measurements conducted for the LPA-containing serum samples were higher after exposure to the developed test than samples without LPA. Early results suggest a limit of detection of 5 μM LPA in serum. The eventual goal is to employ the chemistry described here in a biosensor configuration for the large population-scale, rapid screening of women for the potential occurrence of ovarian cancer.

Determination of a Yellow Rice Toxin, Luteoskyrin, in Rice by Using Liquid Chromatography–Tandem Mass Spectrometry with Electrospray Ionization

2009 ◽  
Vol 72 (6) ◽  
pp. 1321-1326 ◽  
Author(s):  
KOHEI MIZUTANI ◽  
SUSUMU KUMAGAI ◽  
NAOKI MOCHIZUKI ◽  
YASUSHI KITAGAWA ◽  
YOSHIKO SUGITA-KONISHI
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Electrospray Ionization ◽  
High Performance ◽  
Solid Phase ◽  
Multiple Reaction Monitoring ◽  
Phase System ◽  
Ionization Mass ◽  
Solid Phase Extraction Cartridge ◽  
Relative Standard

Penicillium islandicum produces luteoskyrin (LUT), a yellow rice toxin that has been found frequently in rice. However, conventional analytical methods for determining LUT are limited, are complicated, and exhibit low sensitivity. In this study, an analytical method more sensitive and simple based on high-performance liquid chromatography combined with electrospray ionization mass spectrometry was developed. The cleanup procedure of the method was one step, using a solid-phase extraction cartridge. An isocratic mobile-phase system, consisting of acetonitrile–water–acetic acid (50:49:1 [vol/vol/vol]) at a flow rate of 0.2 ml/min, was utilized to obtain the best resolution. Our method showed good linearity (r = 0.9993, 0.5 to 50 ng/g) and high repeatability (relative standard deviation = 8.9 and 5.1% at levels of 0.5 and 10 ng/g, respectively) in the fortification test. The detection and quantification limits for the method in multiple-reaction monitoring mode were 0.1 and 0.3 ng/g, respectively. The average recovery of LUT in spiked rice at 0.5 and 10 ng/g was 80.7 and 85.2%, respectively. The method developed in this study should be applicable to survey LUT in rice, with high sensitivity, selectivity, and rapidity.

SELF-ORGANIZED PHASE SEGREGATION IN A DRIVEN FLOW OF DISSIMILAR PARTICLES MIXTURES

2003 ◽  
Vol 14 (07) ◽  
pp. 955-962 ◽  
Author(s):  
R. B. PANDEY ◽  
J. F. GETTRUST ◽  
RAY SEYFARTH ◽  
LUIS A. CUEVA-PARRA
Keyword(s):  
Gas Phase ◽  
Solid Phase ◽  
Phase Segregation ◽  
Continuous Phase ◽  
Phase System ◽  
Immiscible Fluid ◽  
Fluid Mixture ◽  
Self Organized ◽  
Phase Scaling ◽  
Multi Phase

Self-organized patterns in an immiscible fluid mixture of dissimilar particles driven from a source at the bottom are examined as a function of hydrostatic pressure bias by a Monte Carlo computer simulation. As the upward pressure bias competes with sedimentation due to gravity, a multi-phase system emerges: a dissociating solid phase from the source is separated from a migrating gas phase towards the top by an interface of mixed (bi-continuous) phase. Scaling of solid-to-gas phase with the altitude is nonuniversal and depends on both the range of the height/depth and the magnitude of the pressure bias. Onset of phase separation and layering is pronounced at low bias range.

scholarly journals INVESTIGATION OF EFFECTS OF HEAT RELEASE IN ELECTROCHEMICAL SYSTEMS AND THEIR USE IN TECHNOLOGIES FOR PRODUCTION OF ENERGY-INTENSIVE SOURCES FOR AIRCRAFT

2019 ◽  
Vol 62 (1) ◽  
pp. 46-53 ◽  
Author(s):  
Yuri N. Shalimov ◽  
Igor K. Shuklin ◽  
Vladimir I. Parfenyuk ◽  
Vladimir I. Korolkov ◽  
Alexander V. Russu ◽  
...  
Keyword(s):  
Porous Structure ◽  
Metal Hydride ◽  
Thermal Effects ◽  
Power Plants ◽  
Hydrogen Reduction ◽  
Solid Phase ◽  
Transport Systems ◽  
Phase System ◽  
Electrochemical Systems ◽  
Kinetics Of

The search for new, more energy-intensive types of fuel for the operation of the power plants of aircraft is the most important task in aviation. The unique fuel that has no analogues is hydrogen. The paper attempts to substantiate the technology of metal hydride hydrogen storage in electrochemical systems based on aluminum and its alloys as the most affordable materials from fossil metals, since the traditional methods based on the use of cylinders and cryostats are not effective in transport systems. It is shown that the volumetric storage of hydrogen in the porous structure of metals with the formation of hydrides on atomic bond defects is maximally suitable for the implementation of the system, eliminating the excessive pressure and the low temperatures. The porous structure of the material provides both a high degree of availability of the electrolyte solution to the electrode for the accumulation of hydrides in the entire volume of the metal, and not only on its surface, but also the conditions for the realization of the reduction effect that excludes the explosive nature of hydrogen extraction. The problem of increasing the temperature in the reaction zone, which sometimes causes a slowdown in the rate of certain stages of the electrochemical process, is considered. Using the example of galvanic chrome plating, it has been established that an increase in the temperature inhibits the process of the reducing of the metallic chromium. Therefore, the detailed account of the thermal effects in the electrochemical system allows us to determine the mechanism of the processes. The work revealed that the thermal effects arising at the cathode determine the kinetics of the hydrogen reduction processes during the formation of a hydride. And the thermal effects at the anode determine the kinetics of the formation of a porous structure in the metal. The authors proposed to use the principle of action associated with the transition to the technologies of the volumetric storage of hydrogen in a solid-phase system based on a metal hydride compound for the formation of a new class of aircraft - diaplan.

An improved solid-phase enzyme and luminescent immunoassay system for steroid hormones and digoxin.

1988 ◽  
Vol 34 (12) ◽  
pp. 2521-2523 ◽  
Author(s):  
W Hubl ◽  
G Daxenbichler ◽  
D Meissner ◽  
H J Thiele
Keyword(s):  
Solid Phase ◽  
Protein A ◽  
High Sensitivity ◽  
Phase System ◽  
Microtiter Plates ◽  
Coating Method ◽  
Partial Denaturation ◽  
17 Hydroxyprogesterone ◽  
Antibody Coating

Abstract A uniform solid-phase system has been developed for enzyme (ELISA) or luminescent (LIA) immunoassays for steroids. These assays were improved by (a) irradiating microtiter plates or polystyrene tubes before coating with antibody or Protein A, (b) coating the plastic trays with nonspecific anti-gamma-globulin or Protein A instead of the steroid-specific first antibody, and (c) partial denaturation of the second antibody before coating the plates or tubes with it. Specific antibodies were raised against cortisol, aldosterone, 17-hydroxyprogesterone, and digoxin. Horseradish peroxidase was used as label for the ELISA and aminoethylisoluminol for the LIA. In comparison with the first (specific) antibody coating method we observed some advantages: From 10- to 33-fold lower concentrations of first antibodies were necessary to bind the same amount of steroids; precision was better (CV, 3.8-7.5% vs 6.9-15.5%). The high sensitivity of these assays (0.5-2.0 pg per tube for the steroids) also allows determination of the steroids and digoxin in plasma and saliva.

scholarly journals Identification and properties of reactive sites in protein capable of binding carbon dioxide in a gas-solid phase system.

1977 ◽  
Vol 23 (2) ◽  
pp. 145-152 ◽  
Author(s):  
Hisateru MITSUDA ◽  
Fumio KAWAI ◽  
Aijiro YAMAMOTO ◽  
Fumiaki SUZUKI ◽  
Kenji NAKAJIMA ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Solid Phase ◽  
Phase System
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