A study on ultrastructure of zona radiata during oocyte development of zebrafish (Danio rerio)

Author(s):  
Nazan Deniz Koc
2003 ◽  
Vol 46 (3) ◽  
pp. 433-441 ◽  
Author(s):  
Claudia Angelica da Silva Brandão ◽  
Maria de Fátima Moraes Valentim ◽  
Érica Pellegrini Caramaschi

Macro- and microscopic ovarian features of Hemiodus microlepis, H. ternetzi and H. unimaculatus were analyzed. Based on these features we proposed an ovarian maturation scale. The nine stages of the preliminary macroscopic fieldwork scale were reduced to five after microscopic analysis of ovaries. The microscopic analysis indicated a group-synchronous oocyte development common to the three species that were characterized as iteroparous synchronous spawners with a total spawning type. The remarkable thickness of the zona radiata layer and the large size of vitellogenic oocytes of Hemiodus ternetzi distinguished this species from the others.


Microscopy ◽  
2013 ◽  
Vol 62 (3) ◽  
pp. 377-382 ◽  
Author(s):  
E. F. Kaviani ◽  
N. Shabanipour ◽  
S. B. Mirnategh

Author(s):  
Valerie V. Ernst

During the earliest stage of oocyte development in the limpet, Acmea scutum, Golgi complexes are small, few and randomly dispersed in the cytoplasm. As growth proceeds, the Golgi complexes increase in size and number and migrate to the periphery of the cell. At this time, fibrous structures resembling striated rootlets occur associated with the Golgi complexes. Only one fibrous structure appears to be associated with a Golgi complex.The fibers are periodically cross banded with an average of 4 dense fibrils and 6 lighter fibrils per period (Fig. 1). The cross fibrils have a center to center spacing of about 7 run which appears to be the same as that of the striated rootlets of the gill cilia in this animal.


2019 ◽  
Vol 133 (2) ◽  
pp. 143-155 ◽  
Author(s):  
Vicenç Quera ◽  
Elisabet Gimeno ◽  
Francesc S. Beltran ◽  
Ruth Dolado

2020 ◽  
Vol 5 (1) ◽  
pp. 16-21
Author(s):  
Nina Herlina ◽  
Mulyati ◽  
Yulianita ◽  
Putri Ananda
Keyword(s):  

Ampas tahu mengandung isoflavon genistein dan daidzein yang diduga memiliki efek hipoglikemik. Penelitian ini bertujuan untuk menguji efek hipoglikemik fraksi etil asetat ampas tahu (FEAAT). Ekstraksi isoflavon ampas tahu dilakukan dengan metode refluks menggunakan etanol dan HCl, kemudian fraksinasi menggunakan etil asetat. Efek hipoglikemik FEAAT dibuktikan menggunakan ikan zebra sebagai hewan uji. Kelompok perlakuan terdiri dari kontrol negatif (akuades), FEAAT1 dan FEAAT2 dengan konsentrasi 3,75% dan 5% serta kontrol positif (metformin). Hiperglikemia diinduksi dengan perendaman menggunakan aloksan 0,05% selama 30 menit, glukosa 1% selama 30 menit. Selanjutnya dilakukan pemberian sampel uji selama 120 menit secara berurutan untuk menguji efek hipoglikemik dari sampel uji. Cuplikan darah diambil pada menit ke-0, 30, 60, 90, dan 120 untuk diuji kadar glukosa darah. Hasil uji menunjukkan bahwa terdapat perbedaan yang nyata pada luas Area Under Curve (AUC0-120) kontrol negatif dengan FEAAT2 dan metformin (p < 0,01). Sementara hasil nilai AUC0-120 FEAAT1 tidak berbeda nyata dengan kontrol negatif. FEAAT2 efektif sebagai agen hipoglikemik pada model hewan ikan zebra yang diinduksi hiperglikemik.


2016 ◽  
Vol 15 (10) ◽  
pp. 2171-2179 ◽  
Author(s):  
Jin-Song Zhang ◽  
Yi Huang ◽  
Xiao-Bo Han ◽  
Ting-Lin Huang ◽  
Alice K. Y. Chan

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