scholarly journals Bacteriological quality of cage-cultured abalone Haliotis asinina

2021 ◽  
Vol 4 (2) ◽  
pp. 151-159
Author(s):  
Jhonamie A. Mabuhay-Omar ◽  
Genese Divine B. Cayabo ◽  
Lota A. Creencia
Keyword(s):  
Pathogenic Bacteria ◽  
Microbial Contamination ◽  
Enteric Bacteria ◽  
Salmonella Spp ◽  
Shigella Spp ◽  
Standard Limit ◽  
Normal Standard ◽  
Vibrio Spp ◽  
Foodborne Infections

Abalone is one of the most highly priced seafood delicacies and prepared in various dishes like breaded, soup, steamed and sashimi. They are susceptible to microbial contamination since it is eaten raw sometimes and pathogenic microorganisms can be hazardous to consumers. The present study was carried out to determine the coliform load and the presence of presumptive pathogenic bacteria in cage-cultured abalone in Taytay, Palawan, Philippines. The study was limited to the detection of coliform and some presumptive pathogenic bacteria in different parts of abalone such as gut, gills and mantle. The result of the study revealed that the count of coliforms present in the mantle and gills of abalone falls within the normal standard limit (7 – 21 MPN 100g-1 sample). On the other hand, the gut of abalone was beyond the standard limit (460 MPN 100g-1 sample). Moreover, the gut of abalone harbors Vibrio spp., Salmonella spp. and Shigella spp. and general enteric bacteria. Foodborne infections caused by Vibrio, Salmonella and Shigella are common in Asia.

scholarly journals Assessment of Antibiotics and Fluoride Toothpastes against Pathogenic Bacteria associated with Dental Caries

2018 ◽  
pp. 1-10
Keyword(s):  
Dental Caries ◽  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Significant Activity ◽  
Salmonella Spp ◽  
E Coli ◽  
Shigella Spp ◽  
Test Organisms ◽  
Susceptibility Tests ◽  
Vibrio Spp

Dental caries is the most contagious disease throughout the world. In the present study, a total of 100 dental caries patients were selected and samples from these patients were collected through sterile pincers for microbiological examination. A total of 9 different pathogenic bacterial isolates were identified through conventional culturing technique and among them maximum number of occurrence was by Salmonella spp (26.13%), followed by E. coli (17.39%), Proteus spp (17.39%), Shigella spp (8.69%), Pseudomonas spp (8.69%), Vibrio spp (8.69%), Enterobacter spp (4.34%), Yersinia spp (4.34%) and S. mutans (4.34%). Furthermore, antibiotic susceptibility tests were performed by 9 different commercially available antibiotics i.e. vancomycin, erythromycin, clindamycin, amoxicillin, tetracycline, rifampicin, ampicillin, ticarcillin and metronidazole/silver sulphadizine. Kirby Bauer disc diffusion method was used against test organisms and it was observed that all these antibiotics expressed significant activity against greater part of test organisms but few of them showed resistance to metronidazole/silver sulphadizine. In addition to this, different toothpastes encoded as TP-1, TP-2, TP-3, TP-4 and TP-5 were used at a concentration of 20 mg/mL in order to evaluate their activity against test organisms and it was observed that all these toothpastes showed significant activity against isolated bacteria. It was concluded that all these toothpastes contained fluoride which supports to avoid tooth decay and promote minty fresh breath. Furthermore, it was recommended that avoid usage of too much sweets, chocolates, candies especially in children/adults, and there must be a proper use of toothpastes twice a day at every stage of life. Keywords: Dental Caries, Pathogenic Bacteria, Antibiotics, Fluoride Toothpastes

scholarly journals Microbiological quality assessment for drug resistant pathogenic microorganisms from the fresh vended fruit juices

2017 ◽  
Vol 6 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Nurunnahar Akter Lucky ◽  
Ifra Tun Nur ◽  
Tasnia Ahmed
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Fruit Juices ◽  
Drug Resistant ◽  
Pathogenic Microorganisms ◽  
Salmonella Spp ◽  
Juice Processing ◽  
Nutritive Values ◽  
Shigella Spp ◽  
Vibrio Spp

Fruit juices are very popular due to their freshness and taste. These juices contain all the nutritive values those were present in whole fruits. Fresh juices are healthful drink for people of all ages. But the condition is quite opposite if they become contaminated with pathogenic bacteria during the juice processing. Because of the nutritive properties juice can give enough opportunities to the microorganisms to survive and multiply. In our current study we included eight types of fresh juices to study the microbial load. All the samples were heavily contaminated. Total bacterial and fungal count was found to be up to 3.0×107 cfu/ml and 2.6×106 cfu/ml, respectively. Salmonella spp. and Shigella spp. was present only in one sample. Staphylococcus aureus and Pseudomonas spp. were the most prominent in all of the eight samples. Vibrio spp. was found in six samples ranging from 2.0×102 cfu/ml to 2.9×104 cfu/ml. All the pathogenic isolates showed drug resistance towards the most commonly prescribed antibiotics indicating the risk of difficulty in eradicating diseases.Stamford Journal of Microbiology, Vol.6(1) 2016: 7-10

scholarly journals Prevalence of Pathogenic Bacteria in Shrimp Samples Collected from Hatchery, Local Markets and the Shrimp Processing Plant

2016 ◽  
Vol 29 (1) ◽  
pp. 7-10 ◽  
Author(s):  
M Majibur Rahman ◽  
Farjana Rahman ◽  
Farzana Afroze ◽  
Farzana Yesmin ◽  
Kazi Kaniz Fatema ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Processing Plant ◽  
Salmonella Spp ◽  
Economic Interests ◽  
Local Markets ◽  
E Coli ◽  
Export Quality ◽  
Klebsiella Spp ◽  
Vibrio Spp

Export quality frozen shrimps comprise as one of the major economic interests in Bangladesh. During cultivation or processing and packaging, microbial condition is an important issue to maintain the quality of shrimps. Eight shrimp samples, of which, one from hatchery, 3 from local markets and 4 processed export quality samples were studied for microbiological risk assessment (MRA). One hatchery, three market and two export quality shrimp samples were found to contain bacterial pathogens probationary identified as Escherichia coli, Klebsiella spp., Vibrio spp., Aeromonas spp., Pseudomonas spp., Listeria spp., Shigella-like organisms, Staphylococcus aureus, and Salmonella spp. and two other processed export quality shrimp samples were completely free of pathogens of any type. Surprisingly, the later two processed export quality shrimp samples showed antibacterial activity against E. coli, Klebsiella sp. and Vibrio sp.Bangladesh J Microbiol, Volume 29, Number 1, June 2012, pp 7-10

scholarly journals Study of Microbiological Quality of Fresh Juices Vended in Dhaka City

2019 ◽  
Vol 36 (1) ◽  
pp. 49-53
Author(s):  
Kayseri Afroz ◽  
Tahmina Shammi ◽  
Md Shahidul Kabir
Keyword(s):  
Pathogenic Bacteria ◽  
Bacterial Load ◽  
Microbiological Quality ◽  
Antibiotic Sensitivity ◽  
Multi Drug Resistance ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Street Vendors ◽  
Shigella Spp ◽  
Vibrio Spp

Fresh juices sold by the street vendors are rich in nutritional value and considered as popular drink in Dhaka city .They can be easily contaminated by pathogenic bacteria from unhygienic environment of food preparation area and serves as a potential source of food borne outbreaks. Freshly produced juices of papaya (n = 2), wood apple (n = 2), wood apple and papaya juice (1), lemon (n = 2), and sugarcane (n = 3) were examined for the total bacterial mload, coliforms, Staphylococcus spp., Pseudomonas spp., Vibrio spp., Salmonella spp., and Shigella spp. in this study. Total bacterial load in eight samples were within 0 to 107 cfu/ml, six samples exhibited the presence of staphylococci within the range of 0 to 105 cfu/ml. Total coliforms were detected in six samples which ranged from 104 to 105 cfu/ ml. Vibrio spp., Salmonella spp., and Shigella spp. were isolated by enrichment and selective plating method. Eight samples demonstrated the presence of Vibrio spp. and none of them were positive for Salmonella spp. and Shigella spp. Antibiotic sensitivity patterns were determined against eight isolates using Erythromycin (15μg), Amoxicillin (10 μg), Gentamicin (10 μg), Vancomycin (30 μg), Ciprofloxacin (5 μg), Rifampicin (5 μg), Azithromycin (15 μg), Neomycin (10 μg) and Cefexime (5 μg). Klebsiella sp. (n = 1), Vibrio sp. (n = 2), Enterobacter sp. (n = 1), and Proteus sp. (n = 1) showed multi drug resistance in this study. Contamination of street vended juices pose risks to consumers health and requires adequate attention of appropriate authority. Bangladesh J Microbiol, Volume 36 Number 1 June 2019, pp 49-53

Microbiological Condition of Ground Meat Retailed in Monterrey, Mexico

2001 ◽  
Vol 64 (8) ◽  
pp. 1249-1251 ◽  
Author(s):  
NORMA HEREDIA ◽  
SANTOS GARCÍA ◽  
GUADALUPE ROJAS ◽  
LUCÍA SALAZAR
Keyword(s):  
Microbial Contamination ◽  
Food Poisoning ◽  
Microbiological Quality ◽  
Fecal Coliforms ◽  
Ground Meat ◽  
Fusarium Spp ◽  
Salmonella Spp ◽  
Shigella Spp ◽  
Low Levels

Eighty-eight samples of ground meat were randomly collected from retail stores in the metropolitan area of Monterrey, Mexico, and were analyzed for microbial contamination. Methods were those recommended by the Mexican regulation and/or the U.S. Food and Drug Administration. Over 75% of the samples contained 105 total mesophilic microorganisms per g, and over 40% had 106 total coliforms per g. Fecal coliforms were present in most samples. Staphylococcus aureus was detected in 2.3% of the samples, Salmonella spp. in 11.4%, Listeria spp. in 62%, and L. monocytogenes in 16%. Escherichia coli was detected in 76% of samples, but none was serotype O157:H7. Shigella spp. was not found in any sample. Fusarium spp. and Mucor spp. were detected in 3.4% of the samples, and low levels of yeast in 93%. The microbiological quality of the ground meat analyzed was unsatisfactory, and the product could be an important cause of food poisoning.

scholarly journals Assessment of microbiological quality of municipal supplied water within Dhaka Metropolis

2015 ◽  
Vol 4 (1) ◽  
pp. 28-30
Author(s):  
Rabeya Islam ◽  
Ahmad Kamruzzaman Majumder ◽  
Md Monzer Hossain Sarker
Keyword(s):  
Escherichia Coli ◽  
Water Quality ◽  
Health Risks ◽  
Microbiological Quality ◽  
Salmonella Spp ◽  
Shigella Spp ◽  
Pump Stations ◽  
Klebsiella Spp ◽  
Vibrio Spp

Microbiological health risks related to water quality are persisting issues especially in the developing countries. Present study was conducted to evaluate the microbiological quality of municipal supply water of a defined region within Dhaka metropolis, Bangladesh. A total of 20 water samples were collected from 10 selected pump stations and 10 different consuming points to examine the burden of Salmonella spp., Shigella spp., Citrobactor spp., Escherichia coli, Klebsiella spp., Aeromonas spp. and Vibrio spp. The study also included the evaluation of impacts due to the consumption of municipal water. The results revealed that the quality of municipal supplied water was not microbiologically safe for the consumers and also demonstrated the possible commencement of various diseases including diarrhoea, typhoid, dysentery and others. DOI: http://dx.doi.org/10.3329/sjm.v4i1.22758 Stamford Journal of Microbiology, Vol.4(1) 2014: 28-30

Microbiological quality of a waste stabilization pond effluent used for restricted irrigation in Valle Del Cauca, Colombia

2002 ◽  
Vol 45 (1) ◽  
pp. 139-143 ◽  
Author(s):  
C.A. Madera ◽  
M.R. Peña ◽  
D.D. Mara
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Salmonella Spp ◽  
Helminth Eggs ◽  
Waste Stabilization ◽  
Shigella Spp ◽  
Field Workers ◽  
Valle Del Cauca ◽  
Final Effluent

This paper discusses the applicability of effluent reuse in agriculture after treatment in a series of anaerobic, facultative and maturation ponds. The WSP system is located in Ginebra municipality, a small town in southwest Colombia. The total HRT is 12 days. Several samples of the final effluent were taken over a 55 day period and were analysed for E.coli, Streptococcus spp. and helminth eggs. Some additional grab samples were taken to determine the presence of pathogenic bacteria such as Salmonella spp. and Shigella spp. The results showed that the system was able to remove 4 log units of E.coli, 1 log unit of Streptococcus spp. and 100% of helminth eggs. Meanwhile, Salmonella spp. were detected in the effluent of the facultative pond whilst Shigella spp. were not detected in any sample. The main species of helminth eggs encountered were Taenia spp., Ascaris spp., Trichuris spp., Hymenolepis nana, H. diminuta and Enterobius vermicularis. Removal efficiencies were satisfactory despite the relatively short HRT. Nevertheless, WHO guidelines were slightly surpassed in the case of E.coli for unrestricted irrigation. The helminth egg value was always below the maximum WHO limit. Hence, this effluent can be safely used for restricted irrigation provided that field workers are protected from direct contact with wastewater given the presence of Salmonella spp. in the facultative pond effluent.

scholarly journals Characteristics of pathogenic bacteria isolates associated with the gut and gill of molluscan shellfish, Eastern Cape, South Africa

10.3823/822 ◽  
2018 ◽  
Vol 8 (2) ◽  
Author(s):  
Elizabeth Bosede Famewo ◽  
Anthony Jide Afolayan
Keyword(s):  
Pathogenic Bacteria ◽  
Morphological Characteristics ◽  
Eastern Cape ◽  
Salmonella Spp ◽  
Human Waste ◽  
Bacterial Diseases ◽  
Marine Habitats ◽  
Shigella Spp ◽  
Different Shapes ◽  
Vibrio Spp

Introduction: Infectious diseases attributable to the consumption of raw and lightly cooked molluscan shellfish are caused by bacterial agents that are associated either with human waste disposed into the ocean or those of marine origin. This has led to the increase in prevalence of bacterial diseases and microbial resistance to antibiotics. Method: Samples of P. perna were dissected and homogenized for bacterial isolation. Six bacteria were isolated, identified and evaluated for their antimicrobial susceptibility profiling. Results: The microbial counts obtained revealed significant differences in the pathogenic bacteria populations found in the gut and gill of Perna perna. The morphological characteristics of the isolates were found to be of different shapes. All the isolates were catalase and oxidase positive except Vibrio spp. which was negative to the latter. Chloramphenicol and ciprofloxacillin were the most effective antibiotics against all the isolates, each exhibiting 100% efficacy. The highest resistance to ampicilin was observed in Salmonella spp., Shigella spp., Listeria monocytogenes and Staphylococcus xylosus. Conclusions: The results revealed that marine habitats are reservoirs of bacterial pathogens and there is increased resistance to antibiotic therapy, thereby constituting risks to public health. Therefore, appropriate surveillance measures to safeguard the health of shellfish consumers’ should be established.

scholarly journals Food-Borne Bacteria Associated with Seafoods: A Brief Review

2020 ◽  
Author(s):  
A. Ali ◽  
A. Parisi ◽  
M.C. Conversano ◽  
A. Iannacci ◽  
F. D’Emilio ◽  
...  
Keyword(s):  
Salmonella Spp ◽  
Food Borne ◽  
Shigella Spp ◽  
Control And Prevention ◽  
Seafood Products ◽  
Microbiological Water Quality ◽  
And Storage ◽  
Fish Industry ◽  
Vibrio Spp

Consumption of contaminated seafoods is a major cause of death and hospitalization particularly in poor and developing countries. As with other food types, seafoods are also not free of food-borne pathogens and several risk factors are associated with its consumption. Regarding seafoods, there are regulatory hygienic alerts in importing countries. This paper briefly reviews the occurrence of seafood-borne diseases and describes the most important bacterial causes of these infections. Also, major bacterial threats, the route of infection, and food safety concerns associated with seafoods consumption are explained. Several bacterial pathogens, like Vibrio spp., Salmonella spp., Listeria monocytogenes, Staphylococcus aureus, Clostridium botulinum, Shigella spp., and Aeromonas spp. are considered as microbiological hazards for seafoods. These bacteria can contaminate seafood products anytime from farm to table. Some effective methods should be adopted for control and prevention of bacterial hazards in fish industry. Maintaining the microbiological water quality of domestic capture, post-harvest care, proper hygiene, etc. can minimize the bacterial hazards. Food-borne intoxications can be controlled by the proper refrigeration of seafoods and the continuous monitoring of the chill chain during the entire production process right up to consumption. Other ways to prevent food-borne outbreaks due to consumptions of seafoods are training of the consumers about correct handling of food, proper preparation, and storage of seafoods.

scholarly journals Microbiological quality of frozen black mussels (Mytilus galloprovincialis, Lamarck, 1819) purchased from markets in the İzmir Province of Turkey

2021 ◽  
Vol 38 (2) ◽  
pp. 167-172
Author(s):  
Bülent Kafa ◽  
Berna Kılınç
Keyword(s):  
Fecal Coliform ◽  
Pathogenic Bacteria ◽  
Mytilus Galloprovincialis ◽  
Microbiological Quality ◽  
Coliform Bacteria ◽  
Human Consumption ◽  
Salmonella Spp ◽  
Consumer Safety ◽  
Vibrio Spp

The purpose of this study was to examine the microbiological quality and consumer safety of frozen black mussels purchased from four different markets (A, B, C, D) in the İzmir Province of Turkey. A total of 36 frozen black mussel packages, which contained approximately 50 mussels in each, were purchased from the markets. They were not only examined for total mesophilic, psychrotrophic, coliform and fecal coliform bacteria count but also investigated for pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, Listeria monocytogenes and Vibrio spp. According to the results of the mesophilic bacteria counts, frozen black mussels did not exceed the microbiological limits set for safe consumption. The findings showed that frozen black mussels purchased from (A, B, C, D) of markets were safe for human consumption because pathogenic bacteria species such as E.coli, Salmonella spp., S. aureus, L. monocytogenes and Vibrio spp. were not present in the frozen mussel samples.

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