scholarly journals Monitoring of Outbreaks of Bacterial Cold Water Disease among Ayu in a River Where Asymptomatic Carriers of Flavobacterium psychrophilum Were Released

2010 ◽  
Vol 45 (3) ◽  
pp. 115-120 ◽  
Author(s):  
Akira Kumagai ◽  
Akatsuki Nawata ◽  
Yuichi Taniai
Keyword(s):  
Cold Water ◽  
Asymptomatic Carriers ◽  
Flavobacterium Psychrophilum ◽  
Bacterial Cold Water Disease

scholarly journals Viable but Non-culturable State of Bacterial Cold-water Disease Pathogen Flavobacterium psychrophilum at Various Temperatures

2010 ◽  
Vol 45 (4) ◽  
pp. 158-163 ◽  
Author(s):  
Kazuhiro Sugahara ◽  
Erina Fujiwara-Nagata ◽  
Ayami Fukuda ◽  
Mitsuru Eguchi
Keyword(s):  
Cold Water ◽  
Flavobacterium Psychrophilum ◽  
Bacterial Cold Water Disease

scholarly journals Genetic Diversity of Flavobacterium psychrophilum Isolates from Three Oncorhynchus spp. in the United States, as Revealed by Multilocus Sequence Typing

2016 ◽  
Vol 82 (11) ◽  
pp. 3246-3255 ◽  
Author(s):  
Danielle Van Vliet ◽  
Gregory D. Wiens ◽  
Thomas P. Loch ◽  
Pierre Nicolas ◽  
Mohamed Faisal
Keyword(s):  
United States ◽  
Genetic Diversity ◽  
Rainbow Trout ◽  
Chinook Salmon ◽  
Host Species ◽  
Cold Water ◽  
The United States ◽  
Content Type ◽  
Flavobacterium Psychrophilum ◽  
Bacterial Cold Water Disease

ABSTRACTThe use of a multilocus sequence typing (MLST) technique has identified the intraspecific genetic diversity of U.S.Flavobacterium psychrophilum, an important pathogen of salmonids worldwide. Prior to this analysis, little U.S.F. psychrophilumgenetic information was known; this is of importance when considering targeted control strategies, including vaccine development. Herein, MLST was used to investigate the genetic diversity of 96F. psychrophilumisolates recovered from rainbow trout (Oncorhynchus mykiss), coho salmon (Oncorhynchus kisutch), and Chinook salmon (Oncorhynchus tshawytscha) that originated from nine U.S. states. The isolates fell into 34 distinct sequence types (STs) that clustered in 5 clonal complexes (CCs) (n= 63) or were singletons (n= 33). The distribution of STs varied spatially, by host species, and in association with mortality events. Several STs (i.e., ST9, ST10, ST30, and ST78) were found in multiple states, whereas the remaining STs were localized to single states. With the exception of ST256, which was recovered from rainbow trout and Chinook salmon, all STs were found to infect a single host species. Isolates that were collected during bacterial cold water disease outbreaks most frequently belonged to CC-ST10 (e.g., ST10 and ST78). Collectively, the results of this study clearly demonstrate the genetic diversity ofF. psychrophilumwithin the United States and identify STs of clinical significance. Although the majority of STs described herein were novel, some (e.g., ST9, ST10, ST13, ST30, and ST31) were previously recovered on other continents, which demonstrates the transcontinental distribution ofF. psychrophilumgenotypes.IMPORTANCEFlavobacterium psychrophilumis the causative agent of bacterial cold water disease (BCWD) and rainbow trout fry syndrome (RTFS) and is an important bacterial pathogen of wild and farmed salmonids worldwide. These infections are responsible for large economic losses globally, yet the genetic diversity of this pathogen remains to be fully investigated. Previous studies have identified the genetic diversity of this pathogen in other main aquaculture regions; however, little effort has been focused on the United States. In this context, this study aims to examine the genetic diversity ofF. psychrophilumfrom the United States, as this region remains important in salmonid aquaculture.

scholarly journals Draft Genome Sequences of Six Flavobacterium psychrophilum Strains Isolated from Dead Juvenile Ayu (Plecoglossus altivelis altivelis) near the Mouth of the Nahari River, Kochi, Japan

2019 ◽  
Vol 8 (29) ◽  
Author(s):  
Hazuki Yamashita ◽  
Takayuki Wada ◽  
Yusuke Kato ◽  
Takuji Ikeda ◽  
Masayuki Imajoh
Keyword(s):  
Cold Water ◽  
Draft Genome ◽  
Genome Sequences ◽  
Plecoglossus Altivelis ◽  
Content Type ◽  
Flavobacterium Psychrophilum ◽  
Bacterial Cold Water Disease

Flavobacterium psychrophilum causes bacterial cold-water disease in ayu inhabiting clear rivers in Japan. In this study, we report the draft genome sequences of six F. psychrophilum strains isolated from the gills and skin of dead juvenile ayu near the mouth of the Nahari River.

scholarly journals Relationship between gyrA Mutations and Quinolone Resistance in Flavobacterium psychrophilum Isolates

2004 ◽  
Vol 70 (7) ◽  
pp. 3968-3972 ◽  
Author(s):  
Shotaro Izumi ◽  
Futoshi Aranishi
Keyword(s):  
Amino Acid ◽  
Cold Water ◽  
The United States ◽  
Amino Acid Sequences ◽  
Quinolone Resistance ◽  
Aspartic Acid Residue ◽  
Flavobacterium Psychrophilum ◽  
Bacterial Cold Water Disease ◽  
Rainbow Trout Fry Syndrome ◽  
Susceptibility Patterns

ABSTRACT Flavobacterium psychrophilum is the causative agent of the fish diseases called bacterial cold-water disease and rainbow trout fry syndrome. It has been reported that some isolates of F. psychrophilum are resistant to quinolones; however, the mechanism of this quinolone resistance has been unexplained. In this study, we examined the quinolone susceptibility patterns of 27 F. psychrophilum strains isolated in Japan and the United States. Out of 27 isolates, 14 were resistant to both nalidixic acid (NA) and oxolinic acid (OXA), and the others were susceptible to NA and OXA. When amino acid sequences deduced from gyrA nucleotide sequences of all isolates tested were analyzed, two amino acid substitutions (a threonine residue replaced by an alanine or isoleucine residue in position 83 of GyrA [Escherichia coli numbering] and an aspartic acid residue replaced by a tyrosine residue in position 87) were observed in the 14 quinolone-resistant isolates. These results strongly suggest that, as in other gram-negative bacteria, DNA gyrase is an important target for quinolones in F. psychrophilum.

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