Microorganisms Associated with Valencia Peanut Affected by Pod Rot in New Mexico
Abstract Pod rot of peanut is a disease that occurs worldwide. Soilborne pathogens typically causing pod rot include Rhizoctonia solani, Sclerotium rolfsii, and Pythium spp. Although pod rot is known to occur in New Mexico, no etiological study has been conducted on this disease. In 2005 and 2006, 14 Valencia peanut fields were surveyed in eastern New Mexico where the majority of Valencia peanut is produced. The primary focus of the study was to isolate and identify microorganisms associated with pod rot. The secondary focus of the study was to characterize microorganisms from roots and stems from the same plants selected for pod rot assessment. Peanut plants were collected and processed for isolation of microorganisms by plating seeds, pieces of hull, root, and stem on acidified potato dextrose agar. In both years, the average incidence of pods with hull discoloration or pod rot symptoms varied from 55–60 to over 90%, and disease severity ranged from 5 to over 90% across all fields surveyed. A diverse group of microorganisms representing more than 10 fungal and Oomycete genera were recovered from all plant part tissues, with R. solani as the most predominantly isolated mycelial microorganism. Rhizoctonia solani was found in all fields with average frequency of isolation from approximately 20 to 27% for pod tissue, 19 to 36% for root, and 20 to 27% for stem. In controlled-environment studies, all isolates of R. solani from different plant parts were shown to be pathogenic to Valencia peanut. This etiological study is the first in New Mexico to provide an insight into microorganisms associated with peanut affected by pod rot. Breeding Valencia peanuts for resistance to Rhizoctonia pod rot should be a priority.