scholarly journals ADVANCING NEXT-GENERATION PROTEOMICS: A POLYMER-PATTERNED MICROCHAMBER ENABLES INTEGRATION OF THE DISTINCT PROTEIN SEPARATIONS COMPRISING TWO-DIMENSIONAL ELECTROPHORESIS

2012 ◽  
Author(s):  
A.M. Tentori ◽  
A.J. Hughes ◽  
A.E. Herr
Keyword(s):  
Protein Separations ◽  
Two Dimensional ◽  
Next Generation ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis

Detection of spatially- and stage-specific proteins in extracts from single embryos of the domesticated carrot

Development ◽  
1988 ◽  
Vol 103 (4) ◽  
pp. 665-674
Author(s):  
R.H. Racusen ◽  
F.M. Schiavone
Keyword(s):  
Developmental Stages ◽  
Surgical Techniques ◽  
Protein Separations ◽  
Two Dimensional ◽  
Protein Distribution ◽  
Two Dimensional Electrophoresis ◽  
Sds Page ◽  
Spatial Differences ◽  
Specific Proteins ◽  
Dimensional Electrophoresis

Single embryos, representing each of four distinct morphological stages, were selected from cultures of the domesticated carrot for analysis of total [35S]methionine-labelled proteins. Following exposure to radiolabel for 12 to 18h, embryos were individually disrupted in a 3mm diameter, precisely-matched, plastic mortar and pestle. Radiolabelled proteins extracted by this procedure were separated by two-dimensional electrophoresis procedures, consisting of isoelectric focusing in 1mm tubes, followed by SDS-PAGE in a small slab gel. Comparisons of autoradiographs of these gels revealed that the levels of a number of proteins were modulated during the conversion of disordered callus cells into maturing embryos. In addition, miniature surgical techniques were used to separate the apex (cotyledon end) from the base (root end) of late-stage embryos, for extraction of proteins and analysis of spatial differences in protein distribution. About five proteins in extracts from each section were observed to be synthesized at different rates in the two halves, indicating that there are molecular correlates for early polarized growth. About half of the proteins, whose appearances were unique to apical and basal sections of embryos, were also observed to fluctuate in comparisons of autoradiographs of two-dimensional protein separations from embryos at different developmental stages.

Sialic Acid Dependent Polypeptide Chain Heterogeneity of Human Fibrinogen Demonstrated by Two-Dimensional Electrophoresis

1982 ◽  
Vol 47 (01) ◽  
pp. 019-021 ◽  
Author(s):  
Cemal Kuyas ◽  
André Haeberli ◽  
P Werner Straub
Keyword(s):  
Sialic Acid ◽  
Polypeptide Chain ◽  
Two Dimensional ◽  
Charge Heterogeneity ◽  
Human Fibrinogen ◽  
Two Dimensional Electrophoresis ◽  
Isoelectric Points ◽  
Polypeptide Chains ◽  
Dimensional Electrophoresis ◽  
Chain Type

SummaryHuman fibrinogen was compared with asialofibrinogen by two-dimensional electrophoresis to evaluate the contribution of sialic acid to the heterogeneity of the γ- and Bβ-polypeptide chains.Reduced fibrinogen showed three major variants for both the γ- and Bβ-chains. In addition two minor γ-bands with a more acidic isoelectric point than the normal γ-chains were observed. Electrophoresis in the second dimension (SDS) suggests that these most acidic bands are γ-chain-variants with a higher molecular weight. In asialofibrinogen only two predominant variants with more alkaline isoelectric points were present in each chain type.It is concluded that enzymatic removal of sialic acid partially reduces the heterogeneity of the γ- and Bβ-polypeptide chains of human fibrinogen, but additional sources producing charge heterogeneity must be sought.

Improvement of Two-dimensional Electrophoresis of Rice (Oryza sativaL.) Proteomics

2012 ◽  
Vol 18 (5) ◽  
pp. 819 ◽  
Author(s):  
Yanhua YANG ◽  
Weitong CUI ◽  
Xiaoyong LIU ◽  
Keming ZHU ◽  
Keping CHEN
Keyword(s):  
Two Dimensional ◽  
Two Dimensional Electrophoresis ◽  
Dimensional Electrophoresis
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