scholarly journals Changes in germination ability during genebank storage at some medicinal plant seeds belonging to the Solanaceae family

2001 ◽  
Vol 7 (2) ◽  
Author(s):  
E. Tóth
Keyword(s):  
Medicinal Plant ◽  
Storage Period ◽  
Vegetation Period ◽  
Datura Stramonium ◽  
Potassium Nitrate ◽  
Datura Innoxia ◽  
Hyoscyamus Niger ◽  
Plant Seeds ◽  
Germination Ability ◽  
Solanaceae Family

In the present work we have examined the changes of germination ability of some medicinal plant species belonging to the Solanaceae family (Datura stramonium L., Datura innoxia Mill. and Hyoscyamus niger L.) during 5-6 years' storage period. According to our results, all the three species showed an after-ripening behavior. Potassium nitrate and gibberellic acid increased significantly the rate of germination in the case of Datura species. During the storage period tested (1995-2001), the species maintained their germination ability which is favourably but not significantly influenced by the cooled gene bank conditions. The degree of ripeness affected considerably the proportion of germinated seeds at all the three species. As a consequence of our results we emphasise that propagation with completely ripe seeds is proposed, however, half-ripe seeds developped in an unadvantageous vegetation period can be also utilized in the practice. The effect of vegetation year on stramony seeds manifested in the length of after-ripening period, while in the case of henbane the germination ability was also influenced.

scholarly journals Effects of gamma irradiation on the alkaloid content in seeds of Datura stramonium and the radiosensitivity of derived seedlings

2019 ◽  
Vol 6 (4) ◽  
pp. 533-540
Author(s):  
Nabila Benslimani ◽  
Madjda Khelifi-Slaoui ◽  
Abdelkader Morsli ◽  
Amar Djerrad ◽  
Ezz Al-Dein Al-Ramamneh ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Gamma Rays ◽  
Damage Index ◽  
Tropane Alkaloids ◽  
Datura Stramonium ◽  
Hyoscyamus Niger ◽  
Morphological Variations ◽  
Solanaceae Family

Tropane alkaloids are a group of secondary metabolites occurring naturally in Solanaceae family as Atropa belladona, Datura stramonium, Mandragora officinalis, and Hyoscyamus niger. These molecules have valuable therapeutic applications, for example, atropine and hyoscyamine are utilized as antimuscarinic besides being stomach and intestinal diseases drugs. Plants of the Solanaceae family can provide a natural yet less expensive source of these compounds. Hitherto, in order to emphasize these metabolites biosynthesis, D. stramonium seeds were irradiated using a cobalt-60 source of gamma rays of 5 to 80 Gy and germinated in vitro on MS medium in growth controlled chamber. Mutagenesis of D. stramonium seeds was attempted aiming at obtaining plants from in vitro source that are genetically variable for enhancing the biosynthesis of secondary metabolites, namely alkaloids. Results indicated that D. stramonium seeds exhibited a good radiosensitivity and the mutagen damage index GR (30-50) for D. stramonium was determined at 80 Gy. The Characterization of alkaloids (Atropine and hyoscyamine) was done by infrared spectroscopy which showed that alkaloids content of the irradiated seeds is altered by irradiation as the reference bands were not found with all doses used. In addition, seedlings grown from irradiated in vitro seeds exhibited remarkable morphological variations that varied based on the employed dose of gamma rays. These findings permitted the selection of the optimal irradiation dose (80 Gy) to induce mutations that are likely to prompt changes at genetic and metabolic level of the targeted alkaloids.

scholarly journals Biology of germination of medicinal plant seeds. Part XIXb. Diaspores of Inula helenium L. from Asteraceae family

2015 ◽  
Vol 61 (3) ◽  
pp. 7-12
Author(s):  
Elżbieta Bilińska ◽  
Waldemar Buchwald
Keyword(s):  
Medicinal Plant ◽  
Morphological Features ◽  
Plant Seeds ◽  
Germination Ability ◽  
Inula Helenium ◽  
Asteraceae Family

Summary Morphological features of diaspores and seedlings of Inula helenium L. were described. A four-year cycle of monthly germination analysis was carried out. It was found that accessible light and various temperatures (30°C over 8 hours, 20°C over 16 hours) were optimal for the analysis of Inula helenium fruit germination ability. In these conditions, the percentage of germinated achenes was up to 90%. Diaspores stored in unheated room kept their viability even up to seven years after harvest.

scholarly journals PHENYLPROPANOIDS AND FATTY ACIDS LEVELS IN ROOTS AND LEAVES OF DATURA STRAMONIUM AND DATURA INNOXIA

2017 ◽  
Vol 9 (7) ◽  
pp. 150
Author(s):  
Bilal Rahmoune ◽  
Izzeddine Zakarya Zerrouk ◽  
Abdelkader Morsli ◽  
Madjda Khelifi Slaoui ◽  
Lakhdar Khelifi ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Ferulic Acid ◽  
Caffeic Acid ◽  
Cinnamic Acid ◽  
Sinapic Acid ◽  
Datura Stramonium ◽  
Fatty Acids Composition ◽  
Datura Innoxia ◽  
Max Planck ◽  
Leaves And Roots

Objective: The aim of this research was to determine and compare phenylpropanoids and fatty acids composition in two plant species, Datura innoxia and Datura stramonium.Methods: Phenylpropanoids and fatty acids composition in leaves and roots extracted from Datura innoxia and Datura stramonium, grown under greenhouse conditions, was analyzed by gas chromatography–electron impact/time of flight-mass spectrometry (GC-EI/TOF-MS) chromatography techniques. Analyses were carried out at the Max Planck Institute for Molecular Plant Physiology of Golm (Germany).Results: We revealed that Datura stramonium (DS) contains hydroxy-hexanedioic acid while hexanoic acid was found in Datura innoxia (DI). Also, two fatty acids are common to both Datura species, hexadecanoic acid and octadecanoic acid, with an almost equal rate between leaves and roots. However, phenylpropanoids composition revealed eight compounds; luteolin, quercetin, trans-caffeic acid, trans-ferulic acid, cis-caffeic acid, cis-4-hydroxy-cinnamic acid, trans-4-hydroxy-cinnamic acid and trans-sinapic acid in DI. However, in DS, five compounds were detected: luteolin, quercetin, trans-caffeic acid, trans-ferulic acid and dihydroferulic acid. Also in both Datura species, phenylpropanoids concentration in leaves was significantly higher than in the roots.Conclusion: Our results showed a difference in phenylpropanoids and fatty acids compositions between the two Datura species, with a significantly higher concentration of phenylpropanoids in Datura innoxia than in Datura stramonium

Tropine alkaloids production by hairy root cultures of Datura stramonium and Hyoscyamus niger

1988 ◽  
Vol 27 (2) ◽  
pp. 419-420 ◽  
Author(s):  
Mondher Jaziri ◽  
Marc Legros ◽  
Jacques Homes ◽  
Maurice Vanhaelen
Keyword(s):  
Hairy Root ◽  
Datura Stramonium ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Hyoscyamus Niger

scholarly journals COMPARATIVE INVESTIGATION OF MODELING TECHNIQUES FOR PREDICTION OF STATIC FRICTION COEFFICIENT OF SOME MEDICINAL PLANT SEEDS

2019 ◽  
Vol 16 (32) ◽  
pp. 485-496
Author(s):  
Sahar ASHARI
Keyword(s):  
Friction Coefficient ◽  
Medicinal Plant ◽  
Moisture Content ◽  
Contact Surface ◽  
Static Friction ◽  
Seed Type ◽  
Inference System ◽  
Plant Seeds ◽  
Static Friction Coefficient ◽  
Modeling Techniques

The static friction coefficient (SFC) of some medicinal plant seeds was determined as affected by moisture content and contact surface. Obtained data were subjected to analysis of variance to investigate the effect of main treatments on SFC. Single linear regression (SLR) and intelligent modeling techniques (artificial neural network (ANN) and adaptive neuro-fuzzy inference system (ANFIS)) were applied for SFC prediction based on changes in moisture content, the contact surface, and the seed type. The modeling capability of three model types was compared based on the comparison of the coefficient of determination (R2), root mean square error (RMSE), and mean absolute percentage error (MAPE). The results demonstrated that moisture content, the contact surface, and seed type significantly affected SFC (P0.01). The SFC increased as moisture content increased. Modeling results demonstrated that the best ANFIS model with obtained R2=0.982, RMSE=0.00138, and MAPE=1.258% was more suitable than the best ANN model and 25 individual SLR models for the SFC prediction.

scholarly journals Effect of biostimulant application and long-term storage on the nutritional value of carrot

2019 ◽  
Vol 37 (4) ◽  
pp. 451-457 ◽  
Author(s):  
Elżbieta Wszelaczyńska ◽  
Małgorzata Szczepanek ◽  
Jarosław Pobereżny ◽  
Maciej J Kazula
Keyword(s):  
Vitamin C ◽  
Nutritional Value ◽  
Field Experiments ◽  
Foliar Application ◽  
Plant Protection ◽  
Block Design ◽  
Storage Period ◽  
Vegetation Period ◽  
Total Carotenoids ◽  
Total Sugars

ABSTRACT The nutritional value of carrot is determined by the content of total sugars and monosaccharides, carotenoids and vitamins, including vitamin C, in its roots. In 2009-2011, field experiments were carried out concerning the effect of biostimulating preparations applied during the carrot vegetation period on selected nutrients of its roots (total sugars, monosaccharides, carotenoids and vitamin C). Two biostimulants were foliarly applied in doses of 2 or 3 L ha-1 on various dates. Kelpak SL and Asahi SL: one, two or three applications; where, second and third applied at 2-week interval, additionally Kelpak SL: one application at 4-leaf phase, followed by additional application after 4 weeks. The field study was conducted in a randomized block design with four replications. The treatments of plant protection from diseases and pests complied with carrot requirements. The subject of the research was carrot of the Karotan cultivar. The content of selected components in carrot roots was determined directly after harvest and after six months of storage in the storage chamber with controlled temperature and relative air humidity. The foliar application of Kelpak SL in a single dose (2 L ha-1) at 4-leaf development phase resulted in the significant increase of total sugars, reducing sugars, total carotenoids and vitamin C. A six-month storage period resulted in a decrease of total sugars by 5% and ascorbic acid by 16.8% compared to nutritional values measured right after harvest.

CHEMICAL STUDY OF MEDICINAL PLANT SEEDS

1999 ◽  
pp. 209-218
Author(s):  
S.M. Nolasco ◽  
Olinda E. Quiroga ◽  
B. Wiese ◽  
M.S. Vigo
Keyword(s):  
Medicinal Plant ◽  
Chemical Study ◽  
Plant Seeds

scholarly journals Long-term storage-dependent changes in seed physiological aspects of Hyoscyamus niger from a cold desert region of Trans-Himalaya

2010 ◽  
Vol 1 (2) ◽  
pp. 17
Author(s):  
Shashi Sharma ◽  
Rajender Kumar Sharma
Keyword(s):  
Storage Period ◽  
Himachal Pradesh ◽  
Ambient Conditions ◽  
Hyoscyamus Niger ◽  
No Donor ◽  
Cold Desert ◽  
Tetrazolium Chloride ◽  
Desert Region ◽  
Entire Storage Period ◽  
Chilling Treatment

Henbane (Hyoscyamus niger L.), an economically important medicinal plant, has an endangered status in Himachal Pradesh (India) that needs appropriate conservation interventions. We have examined seed physiological aspects of H. niger from Lahaul (Himachal Pradesh, India), a cold desert region. The freshly harvested seeds exhibited complete dormancy. Gibberellic acid (GA3) and chilling treatment strongly promoted seed germination which was accompanied by increased α-amylase activity. KNO3, NaN3 and sodium nitroprusside (SNP), an NO donor, also promoted germination. During storage, the seeds retained high viability even after a storage of 72 months under ambient conditions. However, they remained dormant during the entire storage period. The responsiveness of seeds to GA3 and chilling treatment gradually declined with progression of storage period. Concomitantly, the triphenyl tetrazolium chloride (TTC) reduction ability of seeds was lowered. The seed responsiveness to KNO3, NaN3 and SNP during storage increased until one year and decreased thereafter. With the progression of the storage period, seeds exhibited elevated lipid peroxidation and reduced catalase activity implying a role of oxidative stress in observed changes. The involvement of phenolics in seed dormancy of H. niger was not evident. The findings are of significance for conservation and cultivation of H. niger through seeds in the arid mountain region.

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