scholarly journals The genetic background of resistance to common bacterial blight in newly identified common bean lines on the basis of inheritance studies

2000 ◽  
Vol 6 (1) ◽  
Author(s):  
E. Mergenthaler ◽  
Bisztray Gy.
Keyword(s):  
Common Bean ◽  
Genetic Background ◽  
Bacterial Blight ◽  
Xanthomonas Campestris ◽  
Resistance Breeding ◽  
Inheritance Of Resistance ◽  
Common Bacterial Blight ◽  
Phaseolus Vulgaris L ◽  
Coupling Phase ◽  
Recessive Genes

Common bacterial blight (CBB), caused by Xanthomonas campestris pv. phaseoli (Xcp). is a major disease problem of common bean (Phaseolus vulgaris L.). The inheritance of resistance in Xrl and Xr2 lines to two isolates of Xcp was studied in the F2 and F3 popu­lations from the crosses between these lines and the Masay variety (susceptible to Xcp). Segregation patterns indicated that different single recessive genes presumably in coupling phase linkage determined the resistance to the HUN and EK-1 1 strains of Xcp in both lines. The presence of some minor, modifying genes beside the monogenic genetic background of resistance was also observed. Xrl and Xr2 lines represent valuable new monogenic genetic sources in resistance breeding to CBB.

scholarly journals Sources of Resistance to Common Bacterial Blight and Rust in Elite Phaseolus vulgaris L. Germplasm

HortScience ◽  
1993 ◽  
Vol 28 (6) ◽  
pp. 644-646 ◽  
Author(s):  
E. Arnaud-Santana ◽  
M.T. Mmbaga ◽  
D.P. Coyne ◽  
J.R. Steadman
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Bacterial Blight ◽  
Xanthomonas Campestris ◽  
Uromyces Appendiculatus ◽  
Common Bacterial Blight ◽  
Phaseolus Vulgaris L ◽  
Sources Of Resistance ◽  
The Common

We studied leaf and pod reactions of 18 Phaseolus vulgaris germplasm lines (three temperate and 15 tropical) to four Xanthomonas campestris pv. phaseoli (XCP) (Smith) Dye strains and seven Uromyces appendiculatus (UA) (Pers.) Unger races. Line × XCP interaction was significant for leaf and pod reactions. The common bean lines XAN-159, BAC-6, and XAN-112 had the best combined leaf and pod resistance to XCP. Line × UA race interactions were significant (P = 0.05). Lines IAPAR-14 and BAC-6 had the best combined resistance to XCP and UA.

Comparison of Seed Transmission and Survival of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans in Common Bean Seeds

2013 ◽  
Vol 14 (1) ◽  
pp. 41 ◽  
Author(s):  
Y. He ◽  
G. P. Munkvold
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Bacterial Blight ◽  
Disease Incidence ◽  
Seed Transmission ◽  
Common Bacterial Blight ◽  
Phaseolus Vulgaris L ◽  
Transmission Frequency ◽  
Xanthomonas Axonopodis ◽  
Seed Health

Xanthomonas axonopodis pv. phaseoli (Smith) Vauterin (Xap) and Xanthomonas fuscans subsp. fuscans Schaad et al. (Xff) cause indistinguishable symptoms known as common bacterial blight of bean (Phaseolus vulgaris L.). These results confirm a higher disease incidence and seed transmission frequency of Xff compared to Xap and reinforce the need for seed health tests that can differentiate the two species. Accepted for publication 21 August 2013. Published 23 September 2013.

Mapping genetic factors affecting the reaction to Xanthomonas axonopodis pv. phaseoli in Phaseolus vulgaris L. under field conditions

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 1046-1056 ◽  
Author(s):  
B Tar'an ◽  
T E Michaels ◽  
K P Pauls
Keyword(s):  
Phaseolus Vulgaris ◽  
Common Bean ◽  
Length Polymorphism ◽  
Bacterial Blight ◽  
Genetic Factors ◽  
Fragment Length Polymorphism ◽  
Segregation Ratio ◽  
Common Bacterial Blight ◽  
Phaseolus Vulgaris L ◽  
Xanthomonas Axonopodis

The objectives of the present study were to evaluate the field effects of Xanthomonas axonopodis pv. phaseoli (Xap), which causes common bacterial blight (CBB) on common bean (Phaseolus vulgaris L.), and to identify genetic factors for resistance to CBB using a linkage map constructed with random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP), simple sequence repeat (SSR), and amplified fragment length polymorphism (AFLP) markers. One hundred and forty-two F2:4 lines, derived from a cross between 'OAC Seaforth' and 'OAC 95-4', and the parents were evaluated for their field reaction to CBB. In the inoculated plots, the reaction to CBB was negatively correlated with seed yield, days to maturity, plant height, hypocotyl diameter, pods per plant, and harvest index. A reduction in seed yield and its components was observed when disease-free and CBB-inoculated plots were compared. The broad-sense heritability estimate of the reaction to CBB was 0.74. The disease segregation ratio was not significantly different from the expected segregation ratio for a single locus in an F2 generation. The major gene for CBB resistance was localized on linkage group (LG) G5. A simple interval mapping procedure identified three genomic regions associated with the reaction to CBB. One quantitative trait loci (QTL), each on LG G2 (BNG71DraI), G3 (BNG21EcoRV), and G5 (PHVPVPK-1) explained 36.3%, 10.2%, and 42.2% of the phenotypic variation for the reaction to CBB, respectively. Together, these loci explained 68.4% of the phenotypic variation. The relative positions of these QTL on the core common bean map and their comparison with the previous QTL for CBB resistance are discussed.Key words: common bean, molecular markers, common bacterial blight.

Gene Estimation, Associations of Traits, and Confirmation of QTL for Common Bacterial Blight Resistance in Common Bean

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 499e-499
Author(s):  
Soon O. Park ◽  
Dermot P. Coyne ◽  
Geunhwa Jung
Keyword(s):  
Common Bean ◽  
Phenotypic Variation ◽  
Bacterial Blight ◽  
Xanthomonas Campestris ◽  
Major Gene ◽  
Flower Color ◽  
Common Bacterial Blight ◽  
Total Phenotypic Variation ◽  
Recombinant Inbred Population ◽  
Serious Disease

Common bacterial blight, incited by Xanthomonas campestris pv. phaseoli (Xcp), is a serious disease of common bean(Phaseolus vulgaris L.). Gene estimation, associations of traits, and confirmation of QTL for resistance to Xcp were investigated in a recombinant inbred population derived from the backcross BC2F6 PC-50 (susceptible to Xcp) × XAN-159 (resistant to Xcp). One or two genes from XAN-159 controlled leaf resistance to Xcp. One major gene from XAN-159 was involved in controlling pod resistance to Xcp. Low (+0.24) to intermediate (+0.57 and +0.75) Pearson correlations were observed between leaf and pod reactions to Xcp. Purple flower color was associated with leaf and pod resistance to Xcp but not days to flower. One to 2 QTLs explained from 20 to 51% of the total phenotypic variation for leaf reactions to 5 Xcp strains. Two QTLs explained from 20 to 22% of the total phenotypic variation for pod reactions to Xcp strains EK-11 and DR-7. A marker BC437.1050 was associated with leaf and pod resistance to 5 Xcp strains in nearly all experiments, and accounted for 13% to 45% of the phenotypic variation for these traits. A unassigned marker D13.1000 was associated with only pod resistance to Xcp strains EK-11 and DR-7. Gene number (1 or 2) estimations and number of QTL (1 or 2) detected for resistance to Xcp generally agree. The confirmed marker BC437.1050 is expected to be useful in breeding programs for resistance to Xcp.

scholarly journals Selecting for Common Bacterial Blight Resistance in Common Bean: Effects of Multiple Leaf Inoculation and Detached Pod Inoculation Test

1998 ◽  
Vol 123 (5) ◽  
pp. 864-867 ◽  
Author(s):  
H.M. Ariyarathne ◽  
D.P. Coyne ◽  
A.K. Vidaver ◽  
K.M. Eskridge
Keyword(s):  
Common Bean ◽  
Bacterial Blight ◽  
Xanthomonas Campestris ◽  
Heritability Estimate ◽  
Accurate Estimate ◽  
Common Bacterial Blight ◽  
Plant Organs ◽  
Disease Reaction ◽  
Split Plot ◽  
Similar Disease

Breeding for resistance is an important strategy to manage common bacterial blight disease caused by Xanthomonas campestris pv. phaseoli (E. Smith) Dye (Xep) in common bean (Phaseolus vulgaris L.). It is necessary to determine if prior inoculation of the first trifoliolate leaf with Xcp influences subsequent reactions in other plant organs by increasing or decreasing resistance to Xcp. It is difficult to get an accurate estimate of heritability of disease reaction in pods since environment greatly affects the heritability estimate if flowering occurs over extended time periods. Thus, the disease reaction in attached pods versus detached pods was compared. A split-split plot design with two replications (growth chambers as blocks) was used, with bean lines as the whole-plot factors, Xcp strains as subplot factors, and bacterial inoculation treatments for leaf reactions or pod treatments as split-split plot factors. The first trifoliolate leaves, later developed leaves, and attached and detached pods were inoculated. No effects of prior inoculation on the disease reactions of subsequently inoculated leaves and pods were observed, indicating that the different plant organs can be inoculated at different times. The fact that detached and attached pods showed similar disease symptoms would suggest use of the former to reduce environment variance and improve heritability estimates of resistance.

scholarly journals Specific Genomic Regions in Common Bean Condition Resistance to Multiple Pathogens

HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 451E-451
Author(s):  
Phillip N. Miklas ◽  
Valerie Stone ◽  
Carlos A. Urrea ◽  
James S. Beaver
Keyword(s):  
Linkage Group ◽  
Common Bean ◽  
Bacterial Blight ◽  
Xanthomonas Campestris ◽  
Recombinant Inbred Lines ◽  
Macrophomina Phaseolina ◽  
Major Effect ◽  
Common Bacterial Blight ◽  
Stem Blight ◽  
Genomic Regions

A genetic linkage map of 170 RAPD markers mapped across 79 recombinant inbred lines (Dorado and XAN-176) reveal genomic regions that condition multiple disease resistance to fungal (Ashy Stem Blight—Macrophomina phaseolina), viral (bean golden mosaic virus—BGMV), and bacterial (common bacterial blight—Xanthomonas campestris pv. phaseoli) pathogens of common bean (Phaseolus vulgaris). A genomic site on linkage group US-1 had a major effect, explaining 18%, 34%, and 40% of the variation in phenotypic reaction to ashy stem blight, BGMV, and common bacterial blight disease, respectively. Adjacent to this region was a QTL conditioning 23% of the variation in reaction to another fungal pathogen, web blight (Thanatephorus cucumeris). A second genomic site on linkage group US-1 had minor affect on multiple resistance expression to the same fungal (15%), viral (15%), and bacterial (10%) pathogens. It is unknown whether these specific genomic regions represent a series of linked QTL affecting resistance to each disease separately or an individual locus with pleiotropic effect against all three pathogens.

scholarly journals Inheritance of Resistance to Common Bacterial Blight in Four Tepary Bean Lines

1999 ◽  
Vol 124 (1) ◽  
pp. 24-27 ◽  
Author(s):  
Carlos A. Urrea ◽  
Phillip N. Miklas ◽  
James S. Beaver
Keyword(s):  
Bacterial Blight ◽  
Xanthomonas Campestris ◽  
Dominant Gene ◽  
Minor Effect ◽  
Inheritance Of Resistance ◽  
Common Bacterial Blight ◽  
Tepary Bean ◽  
Multiple Resistance Genes ◽  
Dominant Genes ◽  
Effect Gene

High levels of resistance to common bacterial blight caused by Xanthomonas campestris pv. phaseoli (Smith) Dye (Xcp) have been observed for tepary bean (Phaseolus acutifolius A. Gray var. latifolius Freeman). However, the inheritance of resistance from this source is unknown for many lines. The inheritance of common bacterial blight resistance was studied in four tepary bean lines crossed with the susceptible tepary bean MEX-114. Progenies were inoculated with a single Xcp strain 484a. Segregation ratios in the F2 generation suggested that resistance in Neb-T-6-s and PI 321637-s was governed by one dominant gene, and Neb T-8a-s had two dominant genes with complementary effects. These hypotheses for inheritance of resistance were supported by various combinations of F1, F3, BC1Pn segregation data in all lines except PI 321637-s where an additional minor-effect gene with recessive inheritance was indicated. Generation means analyses corroborated that multiple resistance genes were present in PI 321638-s. Lack of segregation for susceptibility among testcrosses for allelism between Neb-T-6-s/PI 321637-s, Neb-T-6-s/Neb-T-8a-s, PI 321637-s/Neb-T-8a-s, and PI 321637-s/PI 321638-s, suggested that one or more loci conditioning resistance to common bacterial blight were in common across the four tepary lines.

scholarly journals Confirmation of QTL Associated with Common Bacterial Blight Resistance in Phaseolus vulgaris L.

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 623f-623
Author(s):  
Geunhwa Jung ◽  
Paul W. Skroch ◽  
Dermot P. Coyne ◽  
James Nienhuis ◽  
E. Arnaud-Santana
Keyword(s):  
Common Bean ◽  
Recombinant Inbred ◽  
Bacterial Blight ◽  
Xanthomonas Campestris ◽  
Rapd Marker ◽  
Marker Locus ◽  
Common Bacterial Blight ◽  
Backcross Population ◽  
Transfer Resistance ◽  
Inbred Backcross

Common bacterial blight (CBB) incited by the bacterial pathogen Xanthomonas campestris pv. phaseoli (Smith) Dye is an important disease of common bean. In a previous study, QTL associated with CBB resistance were described based on RAPD marker analysis of a recombinant inbred population derived from the common bean cross BAC-6 (R) × HT-7719 (S) (resistant × susceptible). The objective of this research is to confirm these previously described candidate marker locus-QTL associations using an inbred backcross PC-50 (S) × BAC-6 (R) and a recombinant inbred Venezuela 44 (S) × BAC-6 (R) population. Two markers previously found to be associated with QTL for CBB resistance in the BAC-6 × HT-7719 population were found to account for 30% of the phenotypic variation for CBB resistance in the PC-50 × BAC-6 inbred backcross population. The three most resistant BC2F3 lines based on marker locus genotypes were ranked 1, 3, and 7 (out of 64) based on phenotypic evaluation. These results provide important confirmation of marker locus-QTL associations and indicate that RAPD markers linked to loci controlling the expression of CBB resistance in common bean may be used to transfer resistance genes into susceptible breeding material.

scholarly journals Inheritance of Resistance to Common Bacterial Blight in Tepary Beans

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 839F-840
Author(s):  
A. Dursun ◽  
D.P. Coyne ◽  
M.F. Mohamed ◽  
G. Jung
Keyword(s):  
Phaseolus Vulgaris ◽  
Bacterial Blight ◽  
Xanthomonas Campestris ◽  
Transgressive Segregation ◽  
Inheritance Of Resistance ◽  
Common Bacterial Blight ◽  
Common Beans ◽  
Serious Disease ◽  
Different Strains ◽  
Dominant Genes

Common bacterial blight, incited by the bacterium Xanthomonas campestris pv. phaseoli (Xcp), is a serious disease of common beans [Phaseolus vulgaris (P. v.)]. Some tepary beans (P. acutifolius) are resistant (R) to Xcp and used to breed P. v. with R to Xcp. The objective was to determine the inheritance of the reaction to different strains of Xcp in crosses between susceptible (S) and R tepary lines. The parents, F2, and F3 populations from six tepary crosses involving 3 R × S, 1 R × moderately (M) R, and 2 R × R were inoculated with Xcp strains EK-11, LB-2, and SC-4A. Different single dominant genes controlled the reaction to different Xcp isolates in R × S crosses. Coupling linkage was detected between the genes controlling the reactions to each of the Xcp strains in the crosses NE #4B(s) × NE #19(R) and NE #4B(S) × CIAT-640005(R), except for NE #8A(MR) × NE #4B(S) with strains EK-11 and LB-2 and EK-11 and SC-4A. Transgressive segregation for S was observed in the F2 and F3 NE #8A × NE #8B(R), indicating that the parents possessed different genes for R. No segregation for reactions occurred n the F2 NE #8B × NE #19 and NE #19 × CIAT-640005, indicating that these parents possessed the same genes for R to the three strains.

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