A Comparison of Grain Quantitative Evaluation Performed with Standard Method of Imaging with Light Microscopy and EBSD Analysis

2009 ◽  
Vol 46 (9) ◽  
pp. 454-468 ◽  
Author(s):  
Agnieszka Szczotok ◽  
Maria Sozańnska
Keyword(s):  
Light Microscopy ◽  
Quantitative Evaluation ◽  
Standard Method ◽  
Ebsd Analysis

scholarly journals Quantitative Evaluation of Porosity in Turbine Blades Made of IN713C Superalloy After Hot Isostatic Pressing

2017 ◽  
Vol 62 (1) ◽  
pp. 253-258
Author(s):  
S. Roskosz
Keyword(s):  
Light Microscopy ◽  
Quantitative Evaluation ◽  
Volume Fraction ◽  
Turbine Blades ◽  
Hot Isostatic Pressing ◽  
Quantitative Metallography ◽  
Isostatic Pressing ◽  
Round Shape ◽  
Hip Process ◽  
Increased Pressure

Abstract The aim of this paper is an assessment of the influence of hot isostatic pressing treatment on porosity of cast samples - turbine blades and vane clusters made of the IN713C superalloy. Two variants of HIP treatments, differing in pressure from each other, have been used. The quantitative evaluation of the porosity was performed using light microscopy and quantitative metallography methods. The use of the hot isostatic pressing significantly decreased the volume fraction and size of pores in the test blades, the remaining pores after the HIP process being characterized by a round shape. The increased pressure has caused significant reductions in the area fraction and size of the pores.

scholarly journals A comparison of grain size determination by light microscopy and EBSD analysis

2005 ◽  
Vol 40 (18) ◽  
pp. 4971-4974 ◽  
Author(s):  
N. Gao ◽  
S. C. Wang ◽  
H. S. Ubhi ◽  
M. J. Starink
Keyword(s):  
Grain Size ◽  
Light Microscopy ◽  
Ebsd Analysis ◽  
Size Determination

Cryogenic Light Microscopy and the Development of Long-term Cryopreservation Techniques for Fungi

1994 ◽  
Vol 23 (3) ◽  
pp. 163-167 ◽  
Author(s):  
Vicki Thomas ◽  
David Smith
Keyword(s):  
Light Microscopy ◽  
Cooling Rate ◽  
Standard Method ◽  
Membrane Structure ◽  
Ice Formation ◽  
Freezing And Thawing ◽  
Growth Requirements ◽  
Intracellular Ice ◽  
Cryopreservation Techniques

Since Henry Power in 1663 successfully revived eelworms which had been frozen in vinegar for a few hours, there has been much interest in cryopreservation. As liquid nitrogen and other cooling agents became more widely available a standard method involving a single cooling rate for the cryopreservation of fungi was proposed by Hwang in 1960. This method was used until the early 1980s, but an increasing number of recalcitrant strains were found. Research at the time was empirical and based on attempts to find the universal cryoprotectant which would protect all fungi irrespective of the cooling rate. The real need was to go back to basics and study the response of the living cell to freezing and thawing. The use of cryogenic light microscopy to study fungi provided a major breakthrough, and showed that cooling rates which cause shrinkage or ice formation should be avoided. Further work is required particularly on membrane structure and the mechanism by which intracellular ice causes injury, as well as the effects of the age of the culture, hyphal structure and growth requirements. Cryopreservation is the only method of preservation which will store fungi and other cells for extremely long periods of time without detectable genetic or morphological change.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

The Broad Applications of the Scanning Electron Microscope

1969 ◽  
Vol 27 ◽  
pp. 20-21
Author(s):  
C. T. Nightingale ◽  
S. E. Summers ◽  
T. P. Turnbull
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Light Microscopy ◽  
Surface Topography ◽  
Great Depth ◽  
Resolving Power ◽  
Depth Of Field ◽  
Pictorial Representations ◽  
Scanning Electron

The ease of operation of the scanning electron microscope has insured its wide application in medicine and industry. The micrographs are pictorial representations of surface topography obtained directly from the specimen. The need to replicate is eliminated. The great depth of field and the high resolving power provide far more information than light microscopy.

Tumor Diagnosis

1982 ◽  
Vol 40 ◽  
pp. 262-265
Author(s):  
Bruce Mackay
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Differential Diagnosis ◽  
Light Microscopy ◽  
Clinical Examination ◽  
Ultrastructural Study ◽  
Diagnostic Procedures ◽  
Specific Diagnosis ◽  
Transmission Electron ◽  
Microscopic Diagnosis

The broadest application of transmission electron microscopy (EM) in diagnostic medicine is the identification of tumors that cannot be classified by routine light microscopy. EM is useful in the evaluation of approximately 10% of human neoplasms, but the extent of its contribution varies considerably. It may provide a specific diagnosis that can not be reached by other means, but in contrast, the information obtained from ultrastructural study of some 10% of tumors does not significantly add to that available from light microscopy. Most cases fall somewhere between these two extremes: EM may correct a light microscopic diagnosis, or serve to narrow a differential diagnosis by excluding some of the possibilities considered by light microscopy. It is particularly important to correlate the EM findings with data from light microscopy, clinical examination, and other diagnostic procedures.

Effects of Hyperoxia on Lung Utrastructure

1970 ◽  
Vol 28 ◽  
pp. 26-27
Author(s):  
Gladys Harrison
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Light Microscopy ◽  
Oxygen Effects ◽  
Age Dependent ◽  
The Central Nervous System ◽  
The Subject ◽  
Space Age ◽  
Alveolar Septa ◽  
Extensive Damage

With the advent of the space age and the need to determine the requirements for a space cabin atmosphere, oxygen effects came into increased importance, even though these effects have been the subject of continuous research for many years. In fact, Priestly initiated oxygen research when in 1775 he published his results of isolating oxygen and described the effects of breathing it on himself and two mice, the only creatures to have had the “privilege” of breathing this “pure air”.Early studies had demonstrated the central nervous system effects at pressures above one atmosphere. Light microscopy revealed extensive damage to the lungs at one atmosphere. These changes which included perivascular and peribronchial edema, focal hemorrhage, rupture of the alveolar septa, and widespread edema, resulted in death of the animal in less than one week. The severity of the symptoms differed between species and was age dependent, with young animals being more resistant.

Immunocytochemistry. A Review of Methodology for Electron Microscopic Applicaiton

1974 ◽  
Vol 32 ◽  
pp. 328-329
Author(s):  
Joseph E. Mazurkiewicz
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Ultrastructural Level ◽  
Electron Microscopic ◽  
Biological Interest ◽  
Investigative Approach ◽  
Immunologic Activity ◽  
Dense Label

Immunocytochemistry is a powerful investigative approach in which one of the most exacting examples of specificity, that of the reaction of an antibody with its antigen, isused to localize tissue and cell specific molecules in situ. Following the introduction of fluorescent labeled antibodies in T950, a large number of molecules of biological interest had been studied with light microscopy, especially antigens involved in the pathogenesis of some diseases. However, with advances in electron microscopy, newer methods were needed which could reveal these reactions at the ultrastructural level. An electron dense label that could be coupled to an antibody without the loss of immunologic activity was desired.

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