Micrococcus luteus – An Alternative Test Germ for Testing the Hygienic Performance of Commercial Freshwater Dishwashers

2018 ◽  
Vol 55 (5) ◽  
pp. 369-375 ◽  
Author(s):  
Diana Klapper ◽  
Marc-Kevin Zinn ◽  
Sarah Schulze-Struchtrup ◽  
Britta von Esmarch-Rummler ◽  
Rainer Stamminger
Keyword(s):  
Micrococcus Luteus ◽  
Alternative Test

Study of Alternative Test Method of Internal Arcing by Replacing SF6 with Air in SF6-Insulated Power Equipment

2018 ◽  
Vol 138 (11) ◽  
pp. 914-922
Author(s):  
Masashi Kotari ◽  
Tomo Tadokoro ◽  
Shin-ichi Tanaka ◽  
Mikimasa Iwata
Keyword(s):  
Test Method ◽  
Power Equipment ◽  
Alternative Test ◽  
Alternative Test Method

Pengembangan Instrumen Evaluasi Domain Afektif

1970 ◽  
Vol 13 (1) ◽  
pp. 87-109
Author(s):  
Rohmad Qomari
Keyword(s):  
Instrument Development ◽  
Affective Domain ◽  
Specific Aspect ◽  
Cognitive Domain ◽  
Test Evaluation ◽  
Evaluation Instrument ◽  
Reliable Instrument ◽  
Alternative Test ◽  
Psychomotor Domain

To carry out evaluation comprehensively, we need appropriate instrument that suitable with domain that being evaluated. Evaluation instrument development with test has been conducted by experts. These instruments were only suitable to measure cognitive domain and part of psychomotor domain. To measure affective domain, we need to develop non-test evaluation instrument (alternative test). The development of this instrument tends to more difficult and complicated compare with instrument test evaluation instrument. Therefore, it does necessitate a through study to derive and elaborate affective domain to specific aspect to develop valid and reliable instrument.

An Heuristic for the Construction of Alternative Test Forms for the Zahlen-Verbindungs-Test

1996 ◽  
Vol 82 (1) ◽  
pp. 147-152 ◽  
Author(s):  
Douglas Vickers ◽  
Charles Pearce
Keyword(s):  
Repeated Administration ◽  
Systematic Investigation ◽  
Analytical Problem ◽  
Cognitive Efficiency ◽  
Basic Reason ◽  
Trail Making ◽  
Alternative Test

Trail-making tests such as Oswald and Roth's 1987 Zahlen-Verbindungs-Test offer sensitive and reliable indicators of cognitive efficiency that are also simple, inexpensive, and quick to administer. A disadvantage is that there exist only a limited number of test forms so the scope for repeated administration and systematic investigation is restricted. The basic reason is that the principled generation of such pathways remains an unsolved analytical problem. In this note we propose an heuristic stratagem for the generation of alternative test forms for the Zahlen-Verbindungs-Test.

EFECTO INHIBITORIO DE EXTRACTOS DE Vitex mollis Kunth CONTRA BACTERIAS Y ESPECIES DE Fusarium DE IMPORTANCIA HUMANA Y AGRÍCOLA

2018 ◽  
Vol 41 (4) ◽  
pp. 353-363
Author(s):  
Alberto J. Valencia-Botin ◽  
Melesio Gutiérrez-Lomelí ◽  
Juan A. Morales-Del-Río ◽  
Pedro J. Guerrero-Medina ◽  
Miguel A. Robles-García ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Salmonella Enterica ◽  
Micrococcus Luteus ◽  
E Coli

Actualmente existe la necesidad de hacer frente al problema de la resistencia a los antibióticos y al uso indiscriminado de fungicidas químicos en la agricultura. El objetivo de este trabajo fue evaluar el efecto inhibitorio de extractos acuosos, metanólicos, acetónicos y hexánicos de hoja y tallo de Vitex mollis Kunth (Lamiaceae) contra diferentes bacterias (Escherichia coli, Micrococcus luteus, Salmonella enterica y Staphylococcus aureus) y especies del hongo Fusarium (F. verticillioides, F. oxysporum, F. tapsinum y F. oxysporum f.sp. lycopersici) de importancia en la salud y en la agricultura, así como determinar su composición química general. Se determinaron las concentraciones inhibitorias mínimas (CIM) de todos los extractos por la técnica de microdilución, excepto del hexánico, que no presentó inhibición en las bacterias estudiadas. S. enterica fue la bacteria que mostró mayor sensibilidad al extracto metanólico de tallo (CIM = 28 μg mL-1), le siguieron M. luteus (CIM = 32 μg mL-1), S. aureus (CIM = 75 μg mL-1) y E. coli (CIM = 80 μg mL- 1). Los extractos metanólicos y acuosos de tallo presentaron mayor porcentaje de inhibición contra los diferentes tipos de Fusarium evaluados por el método de dilución en agar. Los extractos de V. mollis inhibieron a F. verticillioides entre 62 y 91 % con 120 μg mL-1 de extracto. El orden de las especies de hongos inhibidas por los extractos fue: F. verticillioides > F. oxysporum > F. tapsinum > F. oxysporum f.sp. lycopersici. La composición química de las especies se determinó mediante pruebas para fenoles, taninos, flavonoides, triterpenos, alcaloides, cumarinas y saponinas. Ninguno de los extractos presentó alcaloides y saponinas. Los fenoles (37.1 mg EAG/g muestra seca) y flavonoides (26.8 mg EQ/g muestra seca) fueron los compuestos mayoritarios en los extractos metanólicos y acuosos. En conclusión, se requieren cantidades muy pequeñas de extracto para la inhibición de bacterias y de Fusarium; por lo tanto, V. mollis puede ser considerada una fuente de metabolitos para este fin y en la agricultura como control alternativo dentro de un manejo integrado de enfermedades.

Construction and Functional Analysis of the Recombinant Bacteriocins Weissellicin-MBF from Weissella confusa MBF8-1

2020 ◽  
Vol 22 (1) ◽  
pp. 115-122
Author(s):  
Amarila Malik ◽  
Elita Yuliantie ◽  
Nisa Yulianti Suprahman ◽  
Theresa Linardi ◽  
Angelina Wening Widiyanti ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Large Scale ◽  
Micrococcus Luteus ◽  
Affinity Column ◽  
Gene Expressions ◽  
His Tag ◽  
Recombinant Peptides ◽  
Weak Activity ◽  
Potential Alternative ◽  
Weissella Confusa

Background: Bacteriocins (Bac1, Bac2, and Bac3) from Weissella confusa MBF8-1, weissellicin- MBF, have been reported as potential alternative substances as well as complements to the existing antibiotics against many antimicrobial-resistant pathogens. Previously, the genes encoded in the large plasmid, pWcMBF8-1, and the spermicidal activity of their synthetic peptides, originally discovered Indonesia, have been studied. Three synthetic bacteriocins peptides of this weissellicin-MBF have been reported for their potential activities, i.e. antibacterial and spermicidal. Objective: The aim of this study was to construct the recombinant Bacteriocin (r-Bac) genes, as well as to investigate the gene expressions and their functional analysis. Method: Here, the recombinant Bacteriocin (r-Bac) genes were constructed and the recombinant peptides (r-Bac1, r-Bac2, and r-Bac3) in B. subtilis DB403 cells were produced on a large scale. After purification, using the His-tag affinity column, their potential bioactivities were measured as well as their antibacterial minimum inhibitory concentrations against Leuconostoc mesenteroides and Micrococcus luteus, were determined. Results: Pure His-tag-recombinant Bac1, Bac2, and Bac3 were obtained and they could inhibit the growth of L. mesenteroides and M. luteus. Conclusion: The recombinant bacteriocin could be obtained although with weak activity in inhibiting gram-positive bacterial growth.

Iso-octenidine: Promising Octenidine Analogue with Improved Solubility

2020 ◽  
Vol 17 ◽  
Author(s):  
Igor K. Yakuschenko ◽  
Nataliya N. Pozdeeva ◽  
Viktoriya A. Mumyatova ◽  
Alexey A. Terentiev ◽  
Svyatoslav Ya. Gadomsky
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Micrococcus Luteus ◽  
Octenidine Dihydrochloride ◽  
First Time

: Iso-octenidine, an isomer of octenidine dihydrochloride, was synthesized and studied for the first time. Isooctenidine was demonstrated to be 3-fold more soluble in water in comparison to original octenidine, and both substances had remarkably similar antibacterial activity (tested on Escherichia Coli and Micrococcus luteus).

Alternative test for detection of coliforms bacteria in manually expressed human milk

2002 ◽  
Vol 78 (3) ◽  
pp. 193-6
Author(s):  
Franz R. Novak ◽  
João Aprígio Guerra de Almeida
Keyword(s):  
Human Milk ◽  
Alternative Test
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