scholarly journals Patogenicidade de fungos entomopatogênicos a mosca-negra-dos-citros, Aleurocanthus woglumi Ashby 1903 (Hemiptera: Aleyrodidae) e Compatibilidade de produtos fitossanitários utilizados na cultura de citros

2019 ◽  
Author(s):  
◽  
Erika Von Nowakonski
Keyword(s):  
Beauveria Bassiana ◽  
Isaria Fumosorosea ◽  
Aleurocanthus Woglumi

A mosca- negra- dos citros Aleurocanthus woglumi (Hemiptera: Aleyrodidae) é uma praga com um grande potencial de dano econômico da citricultura acarretando um prejuízo de 80% na produção dos frutos, gerando um grande impacto na citricultura já que o Brasil é um dos maiores produtores de citros do mundo. O objetivo deste trabalho foi avaliar a patogenicidade de isolados de fungos entomopatogênicos sobre a mosca-negra-dos-citros em condições de laboratório, e o efeito de diferentes produtos fitossanitários utilizados na citricultura sobre o desenvolvimento destes fungos entomopatogênicos. Para o teste de patogenicidade foram utilizadas ninfas de terceiro e quarto ínstares de A. woglumi, sendo a pulverização realizada com uma concentração de 1×108 conídios/ mL dos fungos Beauveria bassiana e Isaria fumosorosea.

Evaluation of the Entomopathogenic Fungi Beauveria bassiana, Metarhizium anisopliae, and Isaria fumosorosea for Control of Sitophilus oryzae

2014 ◽  
Vol 77 (1) ◽  
pp. 87-93 ◽  
Author(s):  
NICKOLAS G. KAVALLIERATOS ◽  
CHRISTOS G. ATHANASSIOU ◽  
MARIA M. AOUNTALA ◽  
DEMETRIUS C. KONTODIMAS
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Galleria Mellonella ◽  
Sitophilus Oryzae ◽  
Exposure Period ◽  
Isaria Fumosorosea ◽  
Stored Grain ◽  
Stored Grain Pest ◽  
Petri Dishes

The entomopathogenic fungi Beauveria bassiana, Metarhizium anisopliae, and Isaria fumosorosea were tested against the stored-grain pest Sitophilus oryzae. The fungi were isolated from the soil (from three locations in Attica, Greece: B. bassiana from Tatoion, M. anisopliae from Marathon, and I. fumosorosea from Aghios Stefanos) using larvae of Galleria mellonella as bait. Suspensions of 2.11 × 107 and 2.11 × 108, 1.77 × 107 and 1.77 × 108, and 1.81 × 107 and 1.81 × 108 conidia per ml of B. bassiana, M. anisopliae, and I. fumosorosea, respectively, were applied by three treatments: (i) sprayed on food and set in petri dishes with adults of S. oryzae, (ii) sprayed on adults of S. oryzae and set in petri dishes without food, and (iii) sprayed on adults of S. oryzae and set in petri dishes with food. The observed mortality of S. oryzae adults during the overall exposure period for the lowest, as well as for the highest, concentrations of B. bassiana, M. anisopliae, and I. fumosorosea ranged from 0 to 100%. Concentration was, in most of the cases tested, a critical parameter that determined the “speed of kill” of the exposed insect species for B. bassiana and M. anisopliae. Conversely, concentration was not that critical for I. fumosorosea, and survival was high in some of the combinations tested, even after 14 days of exposure. Both in the highest and the lowest concentrations of fungi, the mortality of S. oryzae adults was higher when the fungi were applied on adults than when they were applied on food. Higher mortality was observed when food was absent than when food was present, in most of the cases tested. The high efficacy levels recorded in the current study indicate that the tested fungi could be effective biocontrol agents against S. oryzae.

scholarly journals Evaluation of strains of Beauveria bassiana and Isaria fumosorosea to control sugarcane aphids on grain sorghum

age ◽  
2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Karen Harris‐Shultz ◽  
Joseph Knoll ◽  
Somashekhar Punnuri ◽  
Erika Niland ◽  
Xinzhi Ni
Keyword(s):  
Beauveria Bassiana ◽  
Grain Sorghum ◽  
Isaria Fumosorosea

scholarly journals CONSUMO DE APHIS GOSSYPII GLOVER, 1877 E MYZUS PERSICAE (SULZER, 1776) INFECTADOS COM FUNGOS ENTOMOPATOGÊNICOS POR ORIUS INSIDIOSUS (SAY, 1832) (HEMIPTERA, ANTHOCORIDAE)

2008 ◽  
Vol 75 (4) ◽  
pp. 491-498
Author(s):  
E.S. Loureiro ◽  
A. Moino Júnior
Keyword(s):  
Beauveria Bassiana ◽  
Myzus Persicae ◽  
Metarhizium Anisopliae ◽  
Aphis Gossypii ◽  
Orius Insidiosus ◽  
Isaria Fumosorosea ◽  
Lecanicillium Lecanii ◽  
Aphis Gossypii Glover

RESUMO As espécies de pulgões Aphis gossypii e Myzus persicae são cosmopolitas e polífagas, causando prejuízos em plantas cultivadas. Foi avaliado o consumo de A.gossypii e M. persicae, infectados com fungos entomopatogênicos por Orius insidiosus. No centro de placas de Petri foi colocado um disco de 4,5 cm de diâmetro de folha de algodão (para A. gossypii) e um disco de 4,5 cm de diâmetro de folha de pimentão (para M. persicae). A inoculação dos fungos (Beauveria bassiana, Metarhizium anisopliae, Isaria fumosorosea e Lecanicillium lecanii) foi feita através da adição de 1 mL das suspensões fúngicas 1,0 x 105 e 1,0 x 107 conídios/mL sobre a placa de Petri. Os pulgões foram oferecidos a uma fêmea do predador, liberada na placa em diferentes períodos de tempo após a inoculação, além de um tratamento no qual a fêmea do predador foi liberada com os pulgões mortos, já em fase de conidiogênese. As fêmeas do predador foram retiradas após 24 horas da liberação e, em seguida, foi avaliado o consumo de pulgões. O consumo foi significativamente inferior decorridas 24 horas da inoculação, quando comparado com o a testemunha, para as duas espécies de pulgões estudadas. O consumo de pulgões foi menor nas concentrações testadas no período de 24-48 horas que o consumo da testemunha. Após 72 horas da inoculação e após conidiogênese, não foi observado consumo de pulgões pelo predador para as duas concentrações, evidenciando, dessa forma, a capacidade discriminatória do predador, sendo capaz de reconhecer a presa infectada pelo patógeno.

scholarly journals Entomopathogenic Filamentous Fungi as Biocatalysts in Glycosylation of Methylflavonoids

Catalysts ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1148
Author(s):  
Agnieszka Krawczyk-Łebek ◽  
Monika Dymarska ◽  
Tomasz Janeczko ◽  
Edyta Kostrzewa-Susłow
Keyword(s):  
Biological Activity ◽  
Filamentous Fungi ◽  
Beauveria Bassiana ◽  
Biological Activities ◽  
Isaria Fumosorosea ◽  
Methyl Group ◽  
Group 2 ◽  
Pharmacological Application

Flavonoids are known for their numerous biological activities; however, their pharmacological application is limited by poor bioavailability. Glycosides are usually more stable and more soluble in water and in this form, flavonoids are present in nature. Likewise, the presence of the methyl group in the flavonoid skeleton results in facilitated absorption and greater bioavailability. Entomopathogenic filamentous fungi are effective in the biotransformation of flavonoids; they are known especially for efficient glycosylation. In the current study we used strains of Beauveria bassiana KCH J1.5 and Isaria fumosorosea KCH J2 to biotransform flavonoids with a single methyl group. 2′-Hydroxy-5′-methylchalcone was biotransformed by both strains into 2′-hydroxy-5′-methylchalcone 3-O-β-D-(4″-O-methyl)-glucopyranoside. In the culture of B. bassiana KCH J1.5 four products were obtained from 6-methylflavanone: 4′-hydroxy-6-methylflavanone 3′-O-β-D-(4″-O-methyl)-glucopyranoside; 4′-hydroxyflavanone 6-methylene-O-β-D-(4″-O-methyl)-glucopyranoside; 6-hydroxymethylflavanone 3′-O-β-D-(4″-O-methyl)-glucopyranoside and 4′-hydroxy-6-hydroxymethylflavanone 3′-O-β-D-(4″-O-methyl)-glucopyranoside. Biotransformation with I. fumosorosea KCH J2 as a biocatalyst resulted in the formation of 6-methylflavanone 4′-O-β-D-(4″-O-methyl)-glucopyranoside and 2-phenyl-6-methylchromane 4-O-β-D-(4″-O-methyl)-glucopyranoside. All of these flavonoids can be used in biological activity tests and can be useful in studies concerning structure—bioactivity relationships.

scholarly journals Applications of Beauveria bassiana (Hypocreales: Cordycipitaceae) to Control Populations of Spotted Lanternfly (Hemiptera: Fulgoridae), in Semi-Natural Landscapes and on Grapevines

2020 ◽  
Vol 49 (4) ◽  
pp. 854-864
Author(s):  
Eric H Clifton ◽  
Ann E Hajek ◽  
Nina E Jenkins ◽  
Richard T Roush ◽  
John P Rost ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Fourth Instar ◽  
Single Application ◽  
Isaria Fumosorosea ◽  
Public Park ◽  
Fungal Entomopathogens ◽  
Significant Damage ◽  
Lycorma Delicatula ◽  
Natural Landscapes ◽  
Berks County

Abstract Spotted lanternfly, Lycorma delicatula (White), is an invasive Asian insect that was initially found in Berks County, Pennsylvania, in 2014. As of early 2020, this pest had been found in five more eastern states and it is expected to continue to expand its geographical range. Lycorma delicatula is highly polyphagous but seems to prefer tree-of-heaven, Ailanthus altissima. However, grape growers in Pennsylvania have reported significant damage and loss of vines caused by L. delicatula adults. In fall 2018, two fungal entomopathogens (Beauveria bassiana and Batkoa major) drove localized collapses in L. delicatula populations in Berks County, Pennsylvania. In 2019, we tested applications of a commercialized mycoinsecticide based on B. bassiana strain GHA on L. delicatula populations in a public park in southeastern Pennsylvania. A single application of B. bassiana reduced fourth instar nymphs by 48% after 14 d. Applications of B. bassiana to L. delicatula adults in the same park resulted in 43% mortality after 14 d. Beauveria bassiana spores remained viable on foliage for 5–7 d after spraying. We also conducted semi-field bioassays with B. bassiana GHA (formulated as BoteGHA and Aprehend) and another mycoinsecticide containing Isaria fumosorosea Apopka Strain 97 against L. delicatula adults feeding on potted grapes. All the mycoinsecticides killed ≥90% of adults after 9 d using direct applications. Aprehend killed 99% of adults after 9 d with exposure to residues on sprayed grapes. These data show that fungal entomopathogens can help to suppress populations of L. delicatula in agroecosystems and natural areas.

scholarly journals Eficacia de hongos entomopatógenos en el control de Leptopharsa gibbicarina (Hemiptera: Tingidae) en palma de aceite

2016 ◽  
Vol 42 (1) ◽  
pp. 22 ◽  
Author(s):  
Carlos Enrique Barrios T. ◽  
Alex Enrique Bustillo P. ◽  
Kely Lorena Ocampo R. ◽  
Miguel Angel Reina C. ◽  
Hanna Lorena Alvarado M.
Keyword(s):  
Beauveria Bassiana ◽  
Isaria Fumosorosea ◽  
Purpureocillium Lilacinum

Leptopharsa gibbicarina, es una plaga de importancia económica en la palmicultura colombiana. Se evaluó la patogenicidad y virulencia de los hongos Beauveria bassiana (CPBb0404), Isaria fumosorosea (CPIf1001) y Purpureocillium lilacinum (CPPl0601) sobre adultos de L. gibbicarina obtenidos de una cría bajo condiciones controladas. La patogenicidad de los hongos se evaluó en laboratorio, con adultos de L. gibbicarina individualizados en cajas Petri y asperjados con 0,2 ml de una suspensión de 1 x 107 conidias/ml. La virulencia se evaluó en umbráculo en dos etapas, para esto se infestaron hojas de palma con adultos de L. gibbicarina y se asperjaron los hongos en dosis de 1 x 1013 conidias/ha y posteriormente se varió la dosis asperjando el equivalente a 5 x 1012, 1 x 1013 y 1,5 x 1013 conidias/ha. Después los hongos se evaluaron en un lote comercial de palma de aceite. Los esultados del experimento en laboratorio muestra que los tres hongos son patogénicos a L. gibbicarina (> 96% mortalidad). En la evaluacion de la virulencia I. fumosorosea, B. bassiana y P. lilacinum causaron mortalidades del 74,3%, 92,8% y 100%, respectivamente, encontrándose diferencias significativas (P = 0,05). Sin embargo, al evaluar diferentes dosis, no se encontraron diferencias estadísticas entre dosis y hongos evaluados. En la evaluación de los hongos en campo no se encontraron diferencias significativas entre los tratamientos. Este estudio permite recomendar a P. lilacinum para usarlo en programas de manejo integrado para el control de L. gibbicarina debido a que causa altas mortalidades (> 80%) y a los buenos atributos de calidad que presenta en su producción comercial.

Investigation of the internal bacterial flora of Eurygaster integriceps (Hemiptera: Scutelleridae) and pathogenicity of the flora members

Biologia ◽  
2014 ◽  
Vol 69 (10) ◽  
Author(s):  
Ömer Çelebi ◽  
Elif Sevim ◽  
Ali Sevim
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Micrococcus Luteus ◽  
Bacterial Species ◽  
Bacterial Flora ◽  
Molecular Characteristics ◽  
Bacterial Isolates ◽  
Isaria Fumosorosea ◽  
Eurygaster Integriceps ◽  
Rrna Sequencing

AbstractEurygaster integriceps (Hemiptera: Scutelleridae) is one of the most serious pest of wheat and other cereal crops throughout the Near and Middle East including Turkey. To contribute biological control of this pest, we investigated the culturable bacterial flora of E. integriceps and their virulence against it. We also tested different entomopathogenic fungi (Isaria fumosorosea ARSEF8356, Beauveria bassiana ARSEF8356, Metarhizium brunneum ARSEF8671 and Nomurae rileyi ARSEF1670) against this pest under laboratory conditions. Bacterial isolates were characterized based on morphological, biochemical, physiological and molecular characteristics (16S rRNA sequencing). The isolates were identified as Pantoea sp. (S1, S5, S7, S8, S10 and S11), P. agglomerans (S2, S3 and S4), Pseudomonas sp. S6 and Micrococcus luteus S9. The highest mortality within bacterial isolates was observed from Pantoea sp. S1, P. agglomerans S4 and Pantoea sp. S7 with 100%. Mortality of other bacterial species ranged from 33% to 88%. The highest mortality among the tested entomopathogenic fungi was obtained from Isaria fumosorosea ARSEF 8333 with 100%. Mortality of other fungi ranged from 33% to 50%. Consequently, Pantoea sp. S1, P. agglomerans S4, Pantoea sp. S7, Beauveria bassiana ARSEF 8356 and Isaria fumosorosea ARSEF 8356 seem to be promising candidates in the control of E. integriceps.

Liquid culture fermentation for rapid production of desiccation tolerant blastospores of Beauveria bassiana and Isaria fumosorosea strains

2015 ◽  
Vol 127 ◽  
pp. 11-20 ◽  
Author(s):  
Gabriel Moura Mascarin ◽  
Mark Alan Jackson ◽  
Nilce Naomi Kobori ◽  
Robert Warren Behle ◽  
Ítalo Delalibera Júnior
Keyword(s):  
Beauveria Bassiana ◽  
Liquid Culture ◽  
Isaria Fumosorosea ◽  
Rapid Production

scholarly journals Transmission potential of the entomopathogenic fungi Isaria fumosorosea and Beauveria bassiana from sporulated cadavers of Diaphorina citri and Toxoptera citricida to uninfected D. citri adults

BioControl ◽  
2016 ◽  
Vol 61 (5) ◽  
pp. 567-577 ◽  
Author(s):  
Marcos Roberto Conceschi ◽  
Celeste Paola D’Alessandro ◽  
Rafael de Andrade Moral ◽  
Clarice Garcia Borges Demétrio ◽  
Italo Delalibera Júnior
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Diaphorina Citri ◽  
Isaria Fumosorosea ◽  
Transmission Potential ◽  
Toxoptera Citricida
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