scholarly journals Decomposition of Oxalic Acid in Ginger Juice by Adding the Roots of Barley Seedlings Containing Oxalate Oxidase.

1999 ◽  
Vol 46 (5) ◽  
pp. 346-351 ◽  
Author(s):  
Sumitaka YAMASHITA ◽  
Akira KUBOTA ◽  
Naoko FUKAHORI
Keyword(s):  
Oxalic Acid ◽  
Oxalate Oxidase

scholarly journals Oxalic acid degradation by a novel fungal oxalate oxidase from Abortiporus biennis

2016 ◽  
Vol 63 (3) ◽  
Author(s):  
Marcin Grąz ◽  
Kamila Rachwał ◽  
Radosław Zan ◽  
Anna Jarosz-Wilkołazka
Keyword(s):  
Oxalic Acid ◽  
Ph Value ◽  
Enzyme Reaction ◽  
Oxalate Oxidase ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Size Exclusion ◽  
Optimum Temperature ◽  
Intracellular Enzyme ◽  
Exclusion Chromatography

Oxalate oxidase was identified in mycelial extracts of a basidiomycete Abortiporus biennis strain. Intracellular enzyme activity was detected only after prior lowering of the pH value of the fungal cultures by using oxalic or hydrochloric acids. This enzyme was purified using size exclusion chromatography (Sephadex G-25) and ion-exchange chromatography (DEAE-Sepharose). This enzyme exhibited optimum activity at pH 2 when incubated at 40°C, and the optimum temperature was established at 60°C. Among the tested organic acids, this enzyme exhibited specificity only towards oxalic acid. Molecular mass was calculated as 58 kDa. The values of Km for oxalate and Vmax for the enzyme reaction were 0.015 M and 30 mmol min-1, respectively.

Degradation of oxalic acid by transgenic oilseed rape plants expressing oxalate oxidase

Euphytica ◽  
1995 ◽  
Vol 85 (1-3) ◽  
pp. 169-172 ◽  
Author(s):  
C. Thompson ◽  
J. M. Dunwell ◽  
C. E. Johnstone ◽  
V. Lay ◽  
J. Ray ◽  
...  
Keyword(s):  
Oxalic Acid ◽  
Oilseed Rape ◽  
Oxalate Oxidase ◽  
Transgenic Oilseed Rape

Co-immobilization of oxalate oxidase and catalase in films for scavenging of oxygen or oxalic acid

2013 ◽  
Vol 72 ◽  
pp. 96-101 ◽  
Author(s):  
Sandra Winestrand ◽  
Kristin Johansson ◽  
Lars Järnström ◽  
Leif J. Jönsson
Keyword(s):  
Oxalic Acid ◽  
Oxalate Oxidase

Spectrophotometric determination of urinary oxalate with oxalate oxidase prepared from moss.

1980 ◽  
Vol 26 (7) ◽  
pp. 827-830 ◽  
Author(s):  
M F Laker ◽  
A F Hofmann ◽  
B J Meeuse
Keyword(s):  
Hydrogen Peroxide ◽  
Oxalic Acid ◽  
Oxidative Coupling ◽  
Oxalate Oxidase ◽  
Ion Exchange Resins ◽  
Urinary Oxalate ◽  
Moss Species ◽  
Chemical Technique ◽  
Exchange Resins

Abstract A novel spectrophotometric enzymic procedure for estimating oxalic acid in urine is described. Oxalate oxidase, prepared from moss species, converts oxalic acid to hydrogen peroxide and carbon dioxide. Hydrogen peroxide is determined enzymatically with horseradish peroxidase, by oxidative coupling of 3-methyl-2-benzothiazolinone hydrazone with N,N-dimethylaniline; the resulting indamine due is determined spectrophotometrically at 595 nm. Interfering substances are removed by adsorption to ion-exchange resins and oxidation with charcoal, thus avoiding oxalate recovery problems accompanying oxalate isolation. The procedure is rapid, sensitive, linear, and precise. Results agreed well with those obtained with a widely used chemical technique.

Study of Germin Like Oxalate Oxidase Enzyme in Monocot Plants

2019 ◽  
Author(s):  
Satabdi Ghosh ◽  
Debapriya Das ◽  
Piyali Nandy ◽  
Ankita Ray
Keyword(s):  
Oxalic Acid ◽  
Phenylalanine Ammonia Lyase ◽  
Plant Defence ◽  
Oxalate Oxidase ◽  
Systemic Resistance ◽  
Host Immune System ◽  
Acid Oxidase ◽  
Oxidase Enzyme ◽  
Defence Responses ◽  
Plant Defence Responses

Oxalate oxidase enzyme in monocotyledonous plants, act as a scavenger to breakdown oxalic acid into carbon dioxide and hydrogen peroxide. They perform several important functions in early plant defence responses, including induction of PR protein synthesis and systemic resistance. In the present study oxalic acid, the virulence factor of fungi, was applied to monocotyledonous plants and it was observed that it activates the innate host immune system, along with secretion of oxalate oxidase. The enzymatic activities of other host defence related enzymes like catalase, superoxide dismutase, ascorbic acid oxidase, phenolic content, phenylalanine ammonia lyase were compared between the control and oxalic acid treated (2mM) plants.

Spectrophotometric determination of urinary oxalate with oxalate oxidase prepared from moss.

1980 ◽  
Vol 26 (7) ◽  
pp. 827-830 ◽  
Author(s):  
M F Laker ◽  
A F Hofmann ◽  
B J Meeuse
Keyword(s):  
Hydrogen Peroxide ◽  
Oxalic Acid ◽  
Oxidative Coupling ◽  
Oxalate Oxidase ◽  
Ion Exchange Resins ◽  
Urinary Oxalate ◽  
Moss Species ◽  
Chemical Technique ◽  
Exchange Resins

Abstract A novel spectrophotometric enzymic procedure for estimating oxalic acid in urine is described. Oxalate oxidase, prepared from moss species, converts oxalic acid to hydrogen peroxide and carbon dioxide. Hydrogen peroxide is determined enzymatically with horseradish peroxidase, by oxidative coupling of 3-methyl-2-benzothiazolinone hydrazone with N,N-dimethylaniline; the resulting indamine due is determined spectrophotometrically at 595 nm. Interfering substances are removed by adsorption to ion-exchange resins and oxidation with charcoal, thus avoiding oxalate recovery problems accompanying oxalate isolation. The procedure is rapid, sensitive, linear, and precise. Results agreed well with those obtained with a widely used chemical technique.

scholarly journals Determination of Oxalate in Urine, Using an Amperometric Biosensor with Oxalate Oxidase Immobilized on the Surface of a Chromium Hexacyanoferrate-Modified Graphite Electrode

2000 ◽  
Vol 83 (5) ◽  
pp. 1212-1217 ◽  
Author(s):  
Stjepan Milardović ◽  
Zorana Grabarić ◽  
Mihael Tkalčec ◽  
Vlatko Rumenjak
Keyword(s):  
Hydrogen Peroxide ◽  
Oxalic Acid ◽  
Ethylenediaminetetraacetic Acid ◽  
Graphite Electrode ◽  
Oxalate Oxidase ◽  
Amperometric Biosensor ◽  
Urine Samples ◽  
Dialysis Membrane ◽  
Amperometric Method

Abstract A novel enzymatic amperometric method is described for the determination of oxalic acid in urine. An amperometric biosensor was made by immobilizing oxalate oxidase on the surface of a chromium(III) hexacyanoferrate-modified graphite electrode by using a bovine serum albumin and glutaraldehyde cross-linking procedure. The enzyme biocatalyzes oxalate decomposition in the presence of oxygen into carbon dioxide and hydrogen peroxide. The oxalate concentration, which is proportional to the amount of hydrogen peroxide, was determined amperometrically by measuring the current resulting in the reduction of hydrogen peroxide at a very low working potential (0.05 V versus the Hg | Hg2Cl2 | 3M KCl electrode), which minimized the influence of the possible interferences present in human urine. All experiments were performed with succinic buffer, pH 3.8, containing 0.1M KCl and 5.4mM ethylenediaminetetraacetic acid. In an aqueous solution of pure oxalic acid, the biosensor showed good linearity in a concentration range of 2.5–100μM without the use of a dialysis membrane. For untreated urine samples, a high correlation (R2 = 0.9949) was obtained between oxalate concentrations added to urine samples and oxalate recoveries calculated for determinations with the described oxalate biosensor.

The effects of oxalic acid on transgenic Castanea dentata callus tissue expressing oxalate oxidase

Plant Science ◽  
2007 ◽  
Vol 172 (3) ◽  
pp. 488-496 ◽  
Author(s):  
Andreanna Jo Welch ◽  
Arthur John Stipanovic ◽  
Charles Alvin Maynard ◽  
William Allen Powell
Keyword(s):  
Oxalic Acid ◽  
Callus Tissue ◽  
Oxalate Oxidase ◽  
Castanea Dentata
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