scholarly journals Isolation and Identification of Components from Wasabi (Wasabia japonica Matsumura) Flowers and Investigation of Their Antioxidant and Anti-inflammatory Activities

2019 ◽  
Vol 25 (3) ◽  
pp. 449-457
Author(s):  
Takuma Kurata ◽  
Naoki Misawa ◽  
Takahiro Hosoya ◽  
Tomoe Yamada-Kato ◽  
Isao Okunishi ◽  
...  
Keyword(s):  
Isolation And Identification ◽  
Wasabia Japonica ◽  
Anti Inflammatory

scholarly journals Analyzing the Carotenoid Composition of Melilot (Melilotus officinalis (L.) Pall.) Extracts and the Effects of Isolated (All-E)-lutein-5,6-epoxide on Primary Sensory Neurons and Macrophages

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 503
Author(s):  
Györgyi Horváth ◽  
Eszter Csikós ◽  
Eichertné Violetta Andres ◽  
Tímea Bencsik ◽  
Anikó Takátsy ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Sensory Neurons ◽  
Biological Activities ◽  
Water Soluble ◽  
Soluble Form ◽  
Primary Sensory Neurons ◽  
Isolation And Identification ◽  
Melilotus Officinalis ◽  
Carotenoid Composition ◽  
Anti Inflammatory

Melilotus officinalis is known to contain several types of secondary metabolites. In contrast, the carotenoid composition of this medicinal plant has not been investigated, although it may also contribute to the biological activities of the drug, such as anti-inflammatory effects. Therefore, this study focuses on the isolation and identification of carotenoids from Meliloti herba and on the effect of isolated (all-E)-lutein 5,6-epoxide on primary sensory neurons and macrophages involved in nociception, as well as neurogenic and non-neurogenic inflammatory processes. The composition of the plant extracts was analyzed by high performance liquid chromatography (HPLC). The main carotenoid was isolated by column liquid chromatography (CLC) and identified by MS and NMR. The effect of water-soluble lutein 5,6-epoxide-RAMEB (randomly methylated-β-cyclodextrin) was investigated on Ca2+-influx in rat primary sensory neurons induced by the activation of the transient receptor potential ankyrin 1 receptor agonist to mustard-oil and on endotoxin-induced IL-1β release from isolated mouse peritoneal macrophages. (all-E)-Lutein 5,6-epoxide significantly decreased the percent of responsive primary sensory neurons compared to the vehicle-treated stimulated control. Furthermore, endotoxin-evoked IL-1β release from macrophages was significantly decreased by 100 µM lutein 5,6-epoxide compared to the vehicle-treated control. The water-soluble form of lutein 5,6-epoxide-RAMEB decreases the activation of primary sensory neurons and macrophages, which opens perspectives for its analgesic and anti-inflammatory applications.

Antioxidant, Anti-Inflammatory and Cytotoxic Activities of Jasminum multiflorum (Burm. F.) Andrews Leaves towards MCF-7 Breast Cancer and HCT 116 Colorectal Cell Lines and Identification of Bioactive Metabolites

2021 ◽  
Vol 21 ◽  
Author(s):  
Seham Salah El-Hawary ◽  
Hala M. EL-Hefnawy ◽  
Samir Mohamed Osman ◽  
Mohamed A. El-Raey ◽  
Fatma Alzahraa Mokhtar ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Mass Spectroscopy ◽  
Cytotoxic Activities ◽  
Isolation And Identification ◽  
Antioxidant Power ◽  
Hct 116 ◽  
Anti Inflammatory ◽  
Β Carotene ◽  
Mcf 7

Background: The plants of high phenolic contents are perfect antioxidant and anti-inflammatory candidates and participate in biological studies as effective agents towards different cancer cell lines. Objective: To investigate the antioxidant, anti-inflammatory, and cytotoxic activities of the hydromethanolic leaf extract of Jasminum multiflorum (Burm. f.) Andrews. (J. multiflorum), and phenolic profiling of the extract. Methods: The antioxidant activity for the extract was estimated using β-Carotene-linoleic and ferric reducing antioxidant power (FRAP) assays. The anti-inflammatory activity was evaluated by histamine release assay. Cytotoxicity of J. multiflorum was performed using a neutral red uptake assay towards breast cancer (MCF-7) and colorectal cancer (HCT 116) cell lines. Phenolic profiling of the leaves was characterized using high performance liquid chromatography coupled to photodiode array detector-mass spectroscopy-mass spectroscopy (HPLC-PDA-MS/MS), and chromatographic isolation and identification of the isolated compounds were performed using spectroscopic and NMR data, and virtual docking was performed to the isolated compounds against HSP90 (HEAT SHOCK PROTEIN 90). Results : At a concentration of 75 µg mL-1, J. multiflorum extract showed high antioxidant power; 68.23±0.35 % inhibition and 60.30±0.60 a TEAC (µmol Trolox g-1) for β-Carotene-linoleic assay and FRAP assay; respectively, and possessed anti-inflammatory activity with IC50 67.2 µg/ml. J. multiflorum showed high cytotoxic activity with IC50 of 24.81 µg/ml and 11.38 µg/ml for MCF-7 and HCT 116 cell lines, respectively. HPLC-PDA-MS/MS analysis tentatively identified 39 compounds; major compounds are secoiridoid glycosides, kaempferol, and quercetin glycosides, in addition to simple phenylethanoid compounds. Isolation of active metabolites was performed and led to the isolation and identification of four compounds. On the basis of docking study using HSP90 legend, kaempferol neohesperidoside showed a high cytotoxic potential supported by a high affinity score towards HSP90 legend protein. Conclusion: Jasminum multiflorum is a good candidate to isolate cytotoxic agents.

scholarly journals Anti-inflammatory Activity and Phytochemical Profile of Galinsoga Parviflora Cav.

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2133 ◽  
Author(s):  
Elżbieta Studzińska-Sroka ◽  
Marlena Dudek-Makuch ◽  
Justyna Chanaj-Kaczmarek ◽  
Natasza Czepulis ◽  
Katarzyna Korybalska ◽  
...  
Keyword(s):  
Wound Healing ◽  
Endothelial Cells ◽  
Chlorogenic Acid ◽  
Cell Function ◽  
Complete Healing ◽  
Inflammatory Activity ◽  
Isolation And Identification ◽  
Endothelial Cell Function ◽  
Phytochemical Profile ◽  
Anti Inflammatory

The objective of this study was to evaluate the usefulness of a hydroalcoholic extract from Galinsoga parviflora herb (GP) in some aspects of the endothelial cell function necessary for anti-inflammatory activity and wound healing and relate these to the GP phytochemical profile. This study demonstrated that the GP extract caused a dose-dependent reduction of IL-6 secretion on IL-1β-stimulated endothelial cells. The IL-6 release was decreased to 33% ± 9% while this did not influence the IL-6 secretion without stimulation. Additionally, the GP extract exhibited an anti-hyaluronidase activity (IC50 = 0.47 mg/mL), which was evidently stronger than the positive control kaempferol (IC50 = 0.78 mg/mL) as well as a moderate and concentration-dependent, antioxidant activity. The results of the scratch assay showed that exposure of the endothelial cells to GP induced complete healing of the damage after 12 h of the study. The phytochemical profile of the extract was studied by using spectrophotometric (total amount of polyphenols and flavonoids) and UPLC (phenolic acids) methods. The main compound in the GP extract was a chlorogenic acid (2.00 ± 0.01 mg/g by UPLC). The total content of polyphenols was 98.30 ± 0.14 mg of chlorogenic acid equivalent/g of the dry herb and content of flavonoids amounted to 6.15 ± 0.41 mg quercetin equivalent/g of the dry herb. Moreover, the presence of flavonoids in G. parviflora was provided after their isolation and identification by spectroscopic methods. In conclusion, it demonstrated that application of GP in the treatment of skin lesions gives possibility of wound healing based on antioxidant, anti-inflammatory, and hyaluronidase-inhibiting activities of G. parviflora herb extract.

Isolation and Identification of Boswellic Acids by Modern Analytical Techniques

2021 ◽  
pp. 98-112
Author(s):  
Arshiya Zulfeen Mohd Fahim ◽  
Mohammed Shakir Ghouse ◽  
Syed Qumarul Islam ◽  
Mohammed Abdul Mughni Danish ◽  
Shaikh Mehmood ◽  
...  
Keyword(s):  
Experimental Work ◽  
Mineral Acid ◽  
Acid Fraction ◽  
Boswellic Acid ◽  
Isolation And Identification ◽  
Boswellia Serrata ◽  
Boswellic Acids ◽  
Complete Extraction ◽  
Anti Inflammatory ◽  
Work Done

Quality by Design (QbD) is a modern, scientific approach that formalizes product design, automates manual test¬ing, and streamlines troubleshooting. It uses a system¬atic approach to ensure quality by developing a thorough understanding of the compatibility of a finished product to all of the components and processes involved in manufac¬turing that product. Olibanum also known as “Dhup”, Indian Frankincense is an oleo gum resin of Boswellia species. In India it is obtained from Boswellia serrata. Boswellia serrata (Burseraceae) The major use of Boswellia serrata in contemporary medicine is as an anti-arthritic and anti-inflammatory pharmacological agent. The anti-inflammatory properties of the gum resin are attributed to the presence of “boswellic acids” The four major pentacyclic triterpenic acids present in the acidic extract of Boswellia serrata gum resin. β-Boswellic Acid, Acetyl-β-Boswellic Acid, 11-keto-β-Boswellic Acid, Acetyl-11-keto-β-Boswellic Acid. Oleo gum resin was subjected for solubility studies in different solvents. In this 10 commonly available solvents were used for extraction including water. All of them showed same TLC pattern except water. In case of maceration no heat is employed, but the material has to be extracted multiple times with fresh solvent each time. In this method solvent requirement increases. Each time the extract was checked for presence of boswellic acids by TLC to ensure complete extraction. It took 4-5 times repeated extraction for complete extraction of Boswellic acids by maceration. Literature survey reveals that anti-inflammatory activities associated with this resin are completely restricted to presence of Boswellic acids. So focus in experimental work done is placed on isolation of acid fraction of oleo gum resin. As mentioned in the procedures above acid fraction was obtained as white precipitate. This white precipitate was separated, dried and weighed. Amount of acid fraction obtained was determined on weight basis. In the experimental work done isolation of acid fraction was carried out by procedures mentioned in section above. This is common procedure which uses treatment of resin with alkali to convert acid into its salt and then precipitating salt of acid by using mineral acid. Studies were carried out to check variations in amount of acid portion obtained when parameters were altered. Use of 2% KOH followed by dilute hydrochloric acid as mineral acid will be most suitable.

Recent Patents and Discovery of Anti-inflammatory Agents from Marine Source

2019 ◽  
Vol 13 (2) ◽  
pp. 105-114
Author(s):  
Simrin Kapoor ◽  
Namrata Nailwal ◽  
Maushmi Kumar ◽  
Kalyani Barve
Keyword(s):  
Sea Cucumber ◽  
Marine Sponges ◽  
Marine Organisms ◽  
Isolation And Identification ◽  
Inflammatory Conditions ◽  
Marine Source ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Key Terms ◽  
Promising Source

Background: Inflammation has become pathology in the majority of the prevalent diseases such as diabetes, atherosclerosis, epilepsy and neurodegenerative disorders. Anti-inflammatory drugs work wonder in all these conditions, where the patient has become refractory to standard treatment. However, available anti-inflammatory agents have side effects associated with chronic use, thus if we could develop safe and efficacious molecules, quality of health care provided will improve. Since plant sources have been extensively explored, the focus needs to be shifted on the alternative natural sources of anti-inflammatory agents. Water bodies especially the sea and ocean are under investigation to find agents which can tackle inflammation. Objective: This article reviews anti-inflammatory agents obtained from five types of marine organisms namely microalgae, sea cucumber, mussels, sponges and corals. Methods: A literature search was conducted using PubMed/Science Direct with keywords marine organisms, inflammation, marine sponges, sea cucumber, mussels, corals and microalgae. Patents were searched using the key terms inflammation, marine agents from www.google.com/patents, www.uspto.gov, http://espacenet.com, www.freepatentsonline.com, www.wipo.int/pctdb/en/searchsimp. jsp and www.freshpatents.com. Results: Literature and current patents have revealed applications of anti-inflammatory agents from marine organisms in pharmaceuticals and cosmeceuticals. These agents are used to treat inflammatory disorders ranging from minor allergy to chronic conditions like rheumatoid arthritis. Marine waste is also a valuable resource for nutraceuticals and anti-inflammatory agents. Conclusion: The findings reveal that marine organisms could be a promising source of novel antiinflammatory agents. However, further investigations are suggested for the isolation and identification of bioactive, exploring the mechanism of action and evaluating the efficacy in various inflammatory conditions.

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