scholarly journals Identification of Phenolic Compounds in Aquilaria crassna Leaves Via Liquid Chromatography-Electrospray Ionization Mass Spectroscopy

2012 ◽  
Vol 18 (2) ◽  
pp. 259-262 ◽  
Author(s):  
Tetsuro ITO ◽  
Mamoru KAKINO ◽  
Shigemi TAZAWA ◽  
Masayoshi OYAMA ◽  
Hiroe MARUYAMA ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Electrospray Ionization ◽  
Mass Spectroscopy ◽  
Ionization Mass ◽  
Electrospray Ionization Mass Spectroscopy ◽  
Ionization Mass Spectroscopy ◽  
Aquilaria Crassna

scholarly journals FRAGMENTATION STUDIES OF D6,7-ANHIDROERITROMISIN-A BY LIQUID CHROMATOGRAPHY-MASS SPECTROSCOPY (LC-MS)

2010 ◽  
Vol 9 (3) ◽  
pp. 474-478
Author(s):  
Khairan Khairan ◽  
Umar A. Jenie ◽  
Retno S. Sudibyo
Keyword(s):  
Liquid Chromatography ◽  
Electrospray Ionization ◽  
Mass Spectroscopy ◽  
Saccharopolyspora Erythraea ◽  
Wave Number ◽  
Ionization Mass ◽  
Fragmentation Pattern ◽  
Ionization Mass Spectroscopy ◽  
Stretching Vibration ◽  
Two Peaks

Semisynthesis of D6,7-Anhydroerythromycin-A was done by biomodification technique by addition of 0.2% INH into a culture fermentation of Saccharopolyspora erythraea ATCC 11635 in medium Hutchinson. The aim of this research is to studies of fragmentation pattern from new matabolite of D6,7-Anhydroerythromycin-A by Liquid Chromatography-Mass Spectroscopy (LC-MS) and the ionization of mass spectroscopy is use by ESI (Electrospray Ionization) pattern. The FT-IR spectrometric analyzes showed a stretching vibration of C=C conjugated group at wave number 1602.7 cm-1. This C=C conjugated vibration indicated the existence of double bond between C6 and C7 (D6,7), this confirmed that isolate contained D6,7-Anhydroerythromycin-A (the possibility of D6,7 was positive). For complementation, a LC-MS (Liquid Chromatography-Mass Spectroscopy) analyzes using ESI-MS (Electrospray Ionization-Mass Spectroscopy) ionization pattern was conducted to the isolate which resulted Quassimolecular ions [M+H]+ of D7,8- and D6,7-Anhydroerythromycin-A. LC-MS spectrogram of the isolate, which gave two peaks of m/z 732.2460 and m/z 716.2522, confirmed that the m/z 732.2460 possibly was D7,8-Anhydroerythromycin-A, while the m/z 716.2502 and m/z 715.2522 possibly were D6,7-Anhydroerythromycin-A.   Keywords: isoniazid, enoyl reduction, D6,7-Anhidroeritromisin-A, fragmentation, LC-MS.

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