scholarly journals Purification and Characterization of Polyphenol Oxidase from Edible Yam (Dioscorea opposita Thunb.)

2006 ◽  
Vol 12 (3) ◽  
pp. 235-239 ◽  
Author(s):  
Shuji FUJITA ◽  
Yun-Zhe HAN ◽  
Chie KOUNO ◽  
Tomoko MATSUO ◽  
Megumi YAMASHITA ◽  
...  
Keyword(s):  
Polyphenol Oxidase ◽  
Purification And Characterization ◽  
Dioscorea Opposita

Purification and Characterization of Dog-rose (RosadumalisRechst.) Polyphenol Oxidase

1996 ◽  
Vol 44 (10) ◽  
pp. 2982-2986 ◽  
Author(s):  
Halis Şakiroǧlu ◽  
Ömer I. Küfrevioǧlu ◽  
Ismail Kocaçalişkan ◽  
Münir Oktay ◽  
Yavuz Onganer
Keyword(s):  
Polyphenol Oxidase ◽  
Purification And Characterization ◽  
Dog Rose

Partial Purification and Characterization of Latent Polyphenol Oxidase in Iceberg Lettuce (LactucasativaL.)

1996 ◽  
Vol 44 (4) ◽  
pp. 984-988 ◽  
Author(s):  
Soledad Chazarra ◽  
Juana Cabanes ◽  
Josefa Escribano ◽  
Francisco Garcia-Carmona
Keyword(s):  
Polyphenol Oxidase ◽  
Partial Purification ◽  
Purification And Characterization ◽  
Iceberg Lettuce

scholarly journals Study on Purification and Characterization of Polyphenol Oxidase from Acetes chinensis

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7545
Author(s):  
Jianyou Zhang ◽  
Guangcheng Zhou ◽  
Lifeng Fei ◽  
Lifan Chen ◽  
Lei Sun ◽  
...  
Keyword(s):  
Polyphenol Oxidase ◽  
Biochemical Characterization ◽  
Specific Activity ◽  
Deae Cellulose ◽  
Enzymatic Reactions ◽  
Inhibitory Effects ◽  
Purification And Characterization ◽  
Effective Prevention ◽  
Ammonium Sulphate Precipitation

Acetes chinensis (belonging to the Decapoda Sergestidae genus) is widely distributed in East Asian waters and is extremely widespread and present in the shallow coastal areas of China. Polyphenol oxidase (PPO), which was extracted from Acetes chinensis, was purified in a four-step procedure involving phosphate-buffered saline treatment, ammonium sulphate precipitation, DEAE-Cellulose chromatography, and Phenyl-Sepharose HP chromatography, and then, its biochemical characterization was measured. The specific activity of the purified enzyme was increased to 643.4 U/mg, which is a 30.35 times increase in purification, and the recovery rate was 17.9%. L-dopa was used as the substrate, the enzymatic reactions catalyzed by PPO conformed to the Michaelis equation, the maximum reaction velocity was 769.23 U/mL, and the Michaelis constant Km was 0.846 mmol/L. The optimal pH of PPO from Acetes chinensis was 7.5, and the optimal temperature was 35 °C. The metal ions experiment showed that Mn2+ and K+ could enhance the activity of PPO; that Ba2+ and Ca2+ could inhibit the activity of PPO; and that Cu2+ had a double effect on PPO, increasing the PPO activity at low concentrations and inhibiting the PPO activity at high concentrations. The inhibitor experiment showed that the inhibitory effects of EDTA and kojic acid were weak and that ascorbic acid and sodium pyrophosphate had good inhibitory effects. The purification and characterization of Acetes chinensis serve as guidelines for the prediction of enzyme behavior, leading to effective prevention of enzymatic browning during processing.

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