scholarly journals Effects of the White-Skinned Sweet Potato (Ipomoea batatas L.) on the Expression of Adipocytokine in Adipose Tissue of Genetic Type 2 Diabetic Mice

2005 ◽  
Vol 11 (4) ◽  
pp. 369-372 ◽  
Author(s):  
Shuichi KUSANO ◽  
Shogo TAMASU ◽  
Shigekazu NAKATSUGAWA
Keyword(s):  
Adipose Tissue ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Genetic Type ◽  
Diabetic Mice ◽  
Type 2 Diabetic

scholarly journals Chronic phosphodiesterase type 5 inhibition has beneficial effects on subcutaneous adipose tissue plasticity in type 2 diabetic mice

2018 ◽  
Vol 233 (11) ◽  
pp. 8411-8417 ◽  
Author(s):  
Daniela Fiore ◽  
Daniele Gianfrilli ◽  
Silvia Cardarelli ◽  
Fabio Naro ◽  
Andrea Lenzi ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Diabetic Mice ◽  
Beneficial Effects ◽  
Phosphodiesterase Type ◽  
Subcutaneous Adipose ◽  
Type 2 Diabetic

scholarly journals DNA aptamer raised against receptor for advanced glycation end products suppresses renal tubular damage and improves insulin resistance in diabetic mice

2021 ◽  
Vol 18 (1) ◽  
pp. 147916412199053
Author(s):  
Ami Sotokawauchi ◽  
Takanori Matsui ◽  
Yuichiro Higashimoto ◽  
Yuri Nishino ◽  
Yoshinori Koga ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Blood Pressure ◽  
Adipose Tissue ◽  
Tubular Damage ◽  
Diabetic Mice ◽  
Advanced Glycation ◽  
Glycation End Products ◽  
End Products ◽  
Type 2 Diabetic

Objective: Interaction of advanced glycation end products (AGEs) with the receptor RAGE plays a role in diabetic nephropathy. However, effects of RAGE-aptamer on tubular damage remain unknown. We examined whether RAGE-aptamer inhibited tubular damage in KKAy/Ta mice, obese type 2 diabetic mice with insulin resistance. Materials and Methods: Male 8-week-old KKAy/Ta mice received continuous intraperitoneal infusion of either control-aptamer or RAGE-aptamer for 8 weeks. Blood biochemistry and blood pressure, and urinary N-acetyl-β-D-glucosaminidase (NAG) activity and albumin excretion levels were monitored. Kidney and adipose tissue samples were obtained for immunohistochemical analyses. Results: Although RAGE-aptamer did not affect blood glucose, blood pressure, body weight, or serum creatinine values, it significantly inhibited the increase in urinary NAG activity and HOMA-IR in diabetic mice at 12 and 16 and at 16 weeks old, respectively. Furthermore, compared with control-aptamer-treated mice, renal carboxymethyllysine, RAGE, and NADPH oxidase-driven superoxide generation were significantly decreased in RAGE-aptamer-treated mice at 12 weeks old with subsequent amelioration of histological alterations in glomerular and interstitial area, while adipose tissue adiponectin expression was increased. Conclusion: Our present results suggest that RAGE-aptamer could inhibit tubular injury in obese type 2 diabetic mice partly by suppressing the AGE-RAGE-oxidative stress axis and improving insulin resistance.

scholarly journals Caveolin-1 Is Essential for the Improvement of Insulin Sensitivity through AKT Activation during Glargine Treatment on Diabetic Mice

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Hangya Peng ◽  
Panwei Mu ◽  
Haicheng Li ◽  
Shuo Lin ◽  
Chuwen Lin ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Insulin Sensitivity ◽  
Serine Phosphorylation ◽  
Diabetic Mice ◽  
Functional Protein ◽  
Akt Activation ◽  
Caveolin 1 ◽  
Insulin Group ◽  
Type 2 Diabetic

Insulin treatment was confirmed to reduce insulin resistance, but the underlying mechanism remains unknown. Caveolin-1 (Cav-1) is a functional protein of the membrane lipid rafts, known as caveolae, and is widely expressed in mammalian adipose tissue. There is increasing evidence that show the involvement of Cav-1 in the AKT activation, which is responsible for insulin sensitivity. Our aim was to investigate the effect of Cav-1 depletion on insulin sensitivity and AKT activation in glargine-treated type 2 diabetic mice. Mice were exposed to a high-fat diet and subject to intraperitoneal injection of streptozotocin to induce diabetes. Next, glargine was administered to treat T2DM mice for 3 weeks (insulin group). The expression of Cav-1 was then silenced by injecting lentiviral-vectored short hairpin RNA (shRNA) through the tail vein of glargine-treated T2DM mice (CAV1-shRNA group), while scramble virus injection was used as a negative control (Ctrl-shRNA group). The results showed that glargine was able to upregulate the expression of PI3K and activate serine phosphorylation of AKT through the upregulation of Cav-1 expression in paraepididymal adipose tissue of the insulin group. However, glargine treatment could not activate AKT pathway in Cav-1 silenced diabetic mice. These results suggest that Cav-1 is essential for the activation of AKT and improving insulin sensitivity in type 2 diabetic mice during glargine treatment.

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