Geologic setting and interpretation of a zone of middle Tertiary igneous-metamorphic complexes in south-central Arizona

In southern Mexico, discrete ultramafic intrusive bodies larger than 4 km2 are genetically related to their enclosing volcano-sedimentary terranes. These terranes are the Cuicateco and Guerrero, which include the Cuicateco and Tierra Caliente metamorphic complexes, respectively. Their basement is largely unknown, and the ultramafic masses previously have been interpreted as allochthonous dismembered ophiolites. To constrain the age of these accreted terranes, the geologic setting and 40Ar/39Ar ages are presented from the localities of Loma Baya – El Tamarindo, Guerrero, and San Pedro Limón, State of México, in the Tierra Caliente Complex, and Teotitlán – Conceptión Pápalo, Oaxaca, in the Cuicateco Complex. The Teotitlán – Conceptión Pápalo area is characterized by a sequence of metamorphosed intermediate granitoid rocks, andesitic lavas, tuffs, and psammites. The intrusive rocks and lavas are pervasively mylonitized and show abundant ultramafic segregations. The largest ultramafic bodies were emplaced into metatuffs, following the regional east-verging structural trend. Three hornblende separates from a hornblende diorite and one from a hornblende-rich clinopyroxenite yielded plateau ages of ca. 130 Ma. The ultramafic rocks in the Tierra Caliente Complex lack orthopyroxene and transitionally grade outward into hornblende diorites. The El Tamarindo ultramafic body (4.6 km2) rose as a diapir into an Albian volcano-sedimentary sequence. The best estimate for the age of this body is ca. 112 Ma from a hornblende separated from a pegmatite dike sample. This age is similar to the Albian fauna reported at Zihuatanejo and other K–Ar ages from ultramafic rocks at Camalotitos. The rigid and poorly serpentinized San Pedro Limon Stock (15 km2) was vertically emplaced into the Xochipala Formation (Cenomanian) during a regional Neogene transpressive disturbance. Four hornblende samples from hornblende clinopyroxenites and hornblendites yielded remarkably flat age spectra, indicating an age of ca. 105 Ma for the San Pedro Limon Stock. Based on the good correlation between the 40Ar/39Ar ages reported here and earlier faunistic data, it is concluded that the ultramafic rocks cooled penecontemporaneously with the deposition of the enclosing volcano-sedimentary sequences.

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First Report of a New Curtovirus Species, Spinach severe curly top virus, in Commercial Spinach Plants (Spinacia oleracea) from South-Central Arizona

Plant Disease ◽

10.1094/pdis-94-7-0917b ◽

2010 ◽

Vol 94 (7) ◽

pp. 917-917 ◽

Cited By ~ 9

Author(s):

C. Hernandez ◽

J. K. Brown

Keyword(s):

Dna Sequencing ◽

Spinacia Oleracea ◽

The United States ◽

Full Length ◽

Yield Reduction ◽

Insect Vector ◽

First Report ◽

South Central ◽

Total Dna ◽

Central Arizona

During April 2009, a commercial spinach field (1 km2 [250 acres]) in south-central Arizona developed geminivirus-like disease symptoms (4). Approximately 40 to 50% of the spinach plants exhibited extreme leaf distortion, foliar interveinal chlorosis, shortened internodes, and ~80% yield reduction. The beet leafhopper, Circulifer tenellus, the only known insect vector of curtoviruses in the United States, was observed on spinach plants. Total DNA was isolated (1) from three plant samples exhibiting the same symptom phenotype and used to PCR-amplify a 446-bp fragment of a suspected curtovirus, using primers F 5′-CTACCATCAGTAATGATGGG-3′and R 5′ CATATTTGCCACCTCCAGTGTC-3′ designed around the coat protein gene (Cp) for several known curtoviruses. DNA sequencing and BLAST analysis of the cloned fragments (n = 3 with 100% identity) revealed BLAST matches at 81 to 83% with the Cp for three isolates of Beet curly top Iran virus (BCTIV) (EU273816–18). To amplify the full-length curtovirus genome, total DNA from one of the three positive samples was used as the template in rolling circle amplification (RCA) employing the non-sequence specific TempliPhi 100 Amplification System (GE Healthcare) that amplifies circular DNA templates. The RCA products were linearized with PstI, yielding a ~3-Kbp fragment that was cloned into pGEM3zf+ (Promega, Madison, WI). To obtain the complete sequence, one plasmid (09-10-8) containing a full-length insert was selected and prepared for sequencing with the Template Generation System II Kit (Finnzymes, Espoo, Finland). The resultant 28 sequences were assembled into a contig using SeqMan software (DNASTAR, Madison, WI). Also, RCA clones (09.10-2, -3, and -4) from the same sample were subjected to DNA sequencing with universal M13F and M13R primers followed by primer walking (>300 bp overlap). The four 3,066-bp genomes shared 99 to 100% nt identity. An alignment (ClustalV; MegAlign, DNASTAR) with sequences of all curtovirus species available in GenBank indicated that the Arizona spinach isolates shared the highest nt sequence identity (59%) with Horseradish curly top virus (HrCTV). The next closest relatives were Beet mild curly top virus, Beet severe curly top virus, and Spinach curly top virus, at 50%. The genome consists of six open reading frames and lacks the AC3 gene, an arrangement most similar to HrCTV (3). The ICTV approved working cut-off for Curtovirus species demarcation at <89% nt identity (2) supports recognition of this isolate from spinach (GU734126) as a new, previously undescribed curtovirus species, for which we propose the name Spinach severe curly top virus (SSCTV-[Arizona:2009]). The curtovirus-like symptoms, presence of the curtovirus leafhopper vector, and isolation of a curtovirus-like genome from symptomatic spinach plants are highly suggestive of curtovirus etiology. To our knowledge, this is the first report of SSCTV worldwide and its association with diseased spinach in Arizona. Although a different curtovirus species was reported from the same infected spinach field (4), this study provides evidence that at least two curtoviruses were present in this spinach field in Arizona. References: (1) J. J. Doyle and J. L. Doyle. Focus 12:13, 1990. (2) C. M. Fauquet et al. Arch. Virol. 153:783, 2008. (3) K. A. Klute et al. J. Gen. Virol. 77:1369, 1996. (4) C. Nischwitz and M. W. Olsen. Online publication. doi:10.1094/PHP-2010-0216-01-BR. Plant Health Progress. 2010.

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