scholarly journals Monitoring and real-time modeling of Escherichia coli bacteria for the Chattahoochee River, Chattahoochee River National Recreation Area, Georgia, 2000–2019

2020 ◽  
Author(s):  
Brent T. Aulenbach ◽  
Anna M. McKee
Keyword(s):  
Escherichia Coli ◽  
Real Time ◽  
Time Modeling ◽  
Recreation Area ◽  
Chattahoochee River

scholarly journals Association of Ct Values from Real-Time PCR with Culture in Microbiological Clearance Samples for Shiga Toxin-Producing Escherichia coli (STEC)

2020 ◽  
Vol 8 (11) ◽  
pp. 1801
Author(s):  
Michael Bording-Jorgensen ◽  
Brendon D. Parsons ◽  
Gillian A.M. Tarr ◽  
Binal Shah-Gandhi ◽  
Colin Lloyd ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Real Time ◽  
Shiga Toxin ◽  
Real Time Pcr ◽  
Gene Detection ◽  
Culture Positivity ◽  
Hands On ◽  
Culture Independent ◽  
Chromogenic Agar ◽  
Stool Samples

Shiga toxin-producing Escherichia coli (STEC) are associated with acute gastroenteritis worldwide, which induces a high economic burden on both healthcare and individuals. Culture-independent diagnostic tests (CIDT) in frontline microbiology laboratories have been implemented in Alberta since 2019. The objectives of this study were to determine the association between gene detection and culture positivity over time using STEC microbiological clearance samples and also to establish the frequency of specimen submission. Both stx genes’ amplification by real-time PCR was performed with DNA extracted from stool samples using the easyMAG system. Stools were inoculated onto chromogenic agar for culture. An association between gene detection and culture positivity was found to be independent of which stx gene was present. CIDT can provide rapid reporting with less hands-on time and technical expertise. However, culture is still important for surveillance and early cluster detection. In addition, stool submissions could be reduced from daily to every 3–5 days until a sample is negative by culture.

scholarly journals Handling Initial Conditions in Vector Fitting for Real Time Modeling of Power System Dynamics

Energies ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2471
Author(s):  
Tommaso Bradde ◽  
Samuel Chevalier ◽  
Marco De Stefano ◽  
Stefano Grivet-Talocia ◽  
Luca Daniel
Keyword(s):  
Dynamical Systems ◽  
Power System ◽  
System Dynamics ◽  
Real Time ◽  
Initial Conditions ◽  
Test System ◽  
Initial State ◽  
Power System Dynamics ◽  
Vector Fitting ◽  
Time Modeling

This paper develops a predictive modeling algorithm, denoted as Real-Time Vector Fitting (RTVF), which is capable of approximating the real-time linearized dynamics of multi-input multi-output (MIMO) dynamical systems via rational transfer function matrices. Based on a generalization of the well-known Time-Domain Vector Fitting (TDVF) algorithm, RTVF is suitable for online modeling of dynamical systems which experience both initial-state decay contributions in the measured output signals and concurrently active input signals. These adaptations were specifically contrived to meet the needs currently present in the electrical power systems community, where real-time modeling of low frequency power system dynamics is becoming an increasingly coveted tool by power system operators. After introducing and validating the RTVF scheme on synthetic test cases, this paper presents a series of numerical tests on high-order closed-loop generator systems in the IEEE 39-bus test system.

Real-Time PCR Determination of Escherichia coli Genomic DNA Contamination in Plasmid Preparations

2002 ◽  
Vol 301 (1) ◽  
pp. 151-153 ◽  
Author(s):  
Adrián Vilalta ◽  
Vanessa Whitlow ◽  
Terrie Martin
Keyword(s):  
Escherichia Coli ◽  
Real Time ◽  
Real Time Pcr ◽  
Genomic Dna ◽  
Dna Contamination

Real-time PCR for differentiation of F18 variants among enterotoxigenic and Shiga toxin-producing Escherichia coli from piglets with diarrhoea and oedema disease

2013 ◽  
Vol 198 (2) ◽  
pp. 538-540 ◽  
Author(s):  
Jae-Won Byun ◽  
Byeong Yeal Jung ◽  
Ha-Young Kim ◽  
John M. Fairbrother ◽  
Myoung-Heon Lee ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Real Time ◽  
Shiga Toxin ◽  
Real Time Pcr ◽  
Oedema Disease
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