scholarly journals Quantifying Biofield Therapy through Biophoton Emission in a Cellular Model

2020 ◽  
Vol 34 (3) ◽  
pp. 434-454
Author(s):  
Jeremy B Kent
Keyword(s):  
Intervertebral Disc ◽  
Real Time Pcr ◽  
Photon Emission ◽  
Post Treatment ◽  
Cellular Model ◽  
Preclinical Research ◽  
Biofield Therapy ◽  
Collagen Ii ◽  
Photon Multiplier Tube ◽  
Positive Results

Biofield therapy has shown positive results over a broad range of pathology from preclinical research to human studies.  However, biofield therapy investigation is limited by an inability to quantify the therapeutic effect. This study aimed to measure the effects Reiki had on mice intervertebral disc (IVD) cells compared to sham and to quantify Reiki by measuring photon emission. We treated mice IVD cells with ten minutes sessions of either Reiki or sham on three successive days. During treatment, we placed the cells in a specifically constructed box with an installed photon multiplier tube (PMT). Reiki significantly increased the photon emission of the cells post-treatment compared to Reiki pretreatment and sham (P<0.05). Real time PCR showed an increase in collagen II and aggrecan (P <0.05).  We present a means to quantify biofield therapy by measuring the post-treatment photon emission. We concurrently demonstrate Reiki’s effect on the anabolic healing response.

Isolation and identification of Marek’s disease virus (MDV) from feather follicle epithelium and internal organs of diseased chickens in Dakahlia Governorate, Egypt

2019 ◽  
Vol 20 (2) ◽  
pp. 6-11
Author(s):  
Aly El-Kenawy ◽  
Mohamed El-Tholoth ◽  
Emad A
Keyword(s):  
Real Time ◽  
Disease Virus ◽  
Real Time Pcr ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Field Samples ◽  
Feather Follicle ◽  
Positive Results

In the present study, a total of 16 samples including feather follicle epithelium, ovary, spleen and kidney (4 samples for each organ) were collected from diseased chicken flocks suspected to be infected with Marek’s disease virus (MDV) at Dakahlia Governorate, Egypt during the period from October 2016 to October 2017. Each sample was pooled randomly from three to five birds (90 to 360 days old). The isolation of the suspected virus from the collected samples was carried out via chorioallantoic membranes (CAMs) of 12 days old embryonated chicken eggs (ECEs). Three egg passages were carried out for each sample. Hyperimmune serum was prepared against standard MDV. MDV in both field and egg passaged samples (after 3rd passage) was identified by agar gel precipitation test (AGPT) and indirect fluorescence antibody test (IFAT). Molecular identification of virus was carried out by conventional polymerase chain reaction (PCR) and real- time PCR in four selected samples. The results revealed that 14 samples (87.5%) including 4 (100%) samples from feather follicle epithelium, ovary and kidney and 2 (50%) samples from spleen, showed positive results in virus isolation after 3rd passage. The positive results percentage by AGPT for field samples were 50% (8 out of 16 samples), while after the 3rd passage in ECEs were 37.5% (6 out of 16 samples) and the positive results percentage by IFAT for field samples were 62.5% (10 out of 16 samples), while after the 3rd passage in ECEs were 81.25 % (13 out of 16 samples). Viral nucleic acid was detected in all selected samples by conventional and real- time PCR. The results indicate that feather follicle epithelium is the best organ for MDV detection. IFAT is superior over AGPT in virus detection. Conventional and real - time PCR could be efficiently used for molecular detection of the virus.

O2–O3 chemodiscolysis: How much, how long? Retrospective outcome evaluation of different treatment sessions in partially-responder patients

2021 ◽  
pp. 159101992110399
Author(s):  
Federico Bruno ◽  
Nicola Carboni ◽  
Pierpaolo Palumbo ◽  
Francesco Arrigoni ◽  
Marco Varrassi ◽  
...  
Keyword(s):  
Correlation Analysis ◽  
Intervertebral Disc ◽  
Morphological Changes ◽  
Lumbar Disc ◽  
Outcome Evaluation ◽  
Post Treatment ◽  
Injection Group ◽  
Significant Difference ◽  
Disc Area ◽  
Group B

Purpose To retrospectively evaluate the clinical and instrumental outcome of different treatment sessions of oxygen–ozone (O2–O3) chemodiscolysis in patients with lumbar disc herniation Methods We evaluated 73 patients partially responders to a single session of oxygen–ozone (O2–O3) chemodiscolysis and submitted to multiple injections sessions. All patients completed a pre- and post-treatment clinical (VAS and modified McNab score) and instrumental MRI follow-up. Imaging evaluation included assessment of intervertebral disc area (IDA). Pre- and post-treatment differences were compared to evaluate differences in variation between groups. Correlation analysis was used to evaluate the relationship between morphological and clinical parameters. Results Based on the type and number of treatments performed, patients were divided into three groups: Group A) patients submitted to an additional periradicular anaesthetic/steroid injection; Group B) patients submitted to an additional session of intradiscal O2–O3 injection; Group C) patients submitted to two further sessions of intradiscal O2–O3 injection. The results showed an improvement of pain scores in all groups, and a smaller disc area change in group B. Comparing the differences between pre- and post-treatment features among the three different groups of patients, we did not find any statistically significant difference. Correlation analysis did not show any statistically significant correlation between the morphological changes of the intervertebral disc and the clinical output scores. Conclusions In our retrospective observation of partially responder patients, multiple intradiscal ozone injections were not associated with a higher disc shrinkage nor superior clinical outcome compared to a single intradiscal O2–O3 application with an additional periradicular injection session.

scholarly journals Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc

2011 ◽  
Vol 4 (1) ◽  
Author(s):  
Dongrim Seol ◽  
Hyeonghun Choe ◽  
Hongjun Zheng ◽  
Keewoong Jang ◽  
Prem S Ramakrishnan ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Organ Culture ◽  
Real Time ◽  
Real Time Pcr ◽  
Reference Genes ◽  
Quantitative Real Time Pcr ◽  
Selection Of

An approach to the audit of tinnitus management

1995 ◽  
Vol 109 (9) ◽  
pp. 826-829 ◽  
Author(s):  
M. Sadlier ◽  
S. D. G. Stephens
Keyword(s):  
Stress Management ◽  
Internal Consistency ◽  
Clinical Assessment ◽  
Management Programme ◽  
Post Treatment ◽  
Tinnitus Questionnaire ◽  
Good Improvement ◽  
Positive Results

AbstractThis study examines the advantages and internal consistency of an open-ended queionnaire (Benefit problem questionnaire), over the Tinnitus questionnaire used by Jakes et al. (1985) in the auditing of a Stress management Programme.This Tinnitus quesitionnaire showed very little difference pre- and post-treatment, while the Benefit/problem questionnarie showed good improvement over a number of variables. This included some of the more traditional orthogonal values of tinnitus complaint. The clinical assessment made blindly and over different tikme scale to the Benefit/problem questionnaire matched these positive results quite closely.

scholarly journals LIPUS inhibits inflammation and catabolism through the NF‐κB pathway in human degenerative nucleus pulposus cells

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Weiwei Yi ◽  
Qing Chen ◽  
Chuan Liu ◽  
Kaiting Li ◽  
Bailong Tao ◽  
...  
Keyword(s):  
Protein Expression ◽  
Real Time ◽  
Signaling Pathway ◽  
Nucleus Pulposus ◽  
Real Time Pcr ◽  
Inflammatory Factors ◽  
Nucleus Pulposus Cells ◽  
Tnf Α ◽  
Gene And Protein Expression ◽  
Collagen Ii

Abstract Background Low-intensity pulsed ultrasound (LIPUS) is a safe and noninvasive rehabilitative physical therapy with anti-inflammatory effects. The current study investigated the effect of LIPUS on the inflammation of nucleus pulposus (NP) cells and its underlying mechanism. Methods Human NP cells were acquired from lumbar disc herniation tissue samples and cultured for experiments. Human NP cells were treated with LPS and then exposed to LIPUS (15 mW/cm2, 30 mW/cm2 and 60 mW/cm2) for 20 min daily for 3 days to determine the appropriate intensity to inhibit the expression of the inflammatory factors TNF-α and IL-1β. The gene and protein expression of aggrecan, collagen II, MMP-3 and MMP-9 was measured by real‐time PCR and western blotting, respectively. The activity of the nuclear factor‐kappa B (NF‐κB) pathway was examined by western blotting and immunofluorescence. After pretreatment with the NF-κB inhibitor PDTC, the expression of TNF-α, IL-1β, MMP-3 and MMP-9 was measured by real‐time PCR. Results LIPUS at intensities of 15 mW/cm2, 30 mW/cm2 and 60 mW/cm2 inhibited LPS-induced NP cell expression of the inflammatory factors TNF-α and IL-1β, especially at 30 mW/cm2. LIPUS significantly upregulated the gene and protein expression of aggrecan and collagen II and downregulated the gene and protein expression of MMP-3 and MMP-9 in LPS-induced NP cells. The NF‐κB signaling pathway was inhibited by LIPUS through inhibiting the protein expression of p-P65 and the translocation of P65 into the nucleus in LPS-induced NP cells. In addition, LIPUS had similar effects as the NF-κB inhibitor PDTC by inhibiting the NF-κB signaling pathway, inflammation and catabolism in LPS-induced human degenerative nucleus pulposus cells. Conclusion LIPUS inhibited inflammation and catabolism through the NF‐κB pathway in human degenerative nucleus pulposus cells.

Intervertebral Disc Function Is Restored in an In Vivo Model of Chronic Nucleus Pulposus Glycosaminoglycan Reduction

2008 ◽  
Author(s):  
John I. Boxberger ◽  
Joshua D. Auerbach ◽  
Sounok Sen ◽  
George R. Dodge ◽  
Dawn M. Elliott
Keyword(s):  
Intervertebral Disc ◽  
Nucleus Pulposus ◽  
Disc Degeneration ◽  
Lumbar Disc ◽  
Post Treatment ◽  
In Vivo Model ◽  
Long Term Effects ◽  
Early Human

Reduced nucleus pulposus glycosaminoglycan (GAG) content is one of the earliest clinically detectable changes during the course of intervertebral disc degeneration [1,2]. Depletion of nucleus GAG by small percentages consistent with this early loss has been experimentally linked to altered motion segment mechanical function, and thus, potentially increases the risk of damage accumulation directly due to elevated stresses and strains and through altered cellular function [3]. Recently, our laboratory has established an in vivo model in a rat lumbar disc which moderately decreases nucleus GAG to levels observed in early human degeneration. In this model, GAG loss is accompanied by a state of hypermobility at both 4 and 12 weeks post treatment [4], potentially making the disc susceptible to mechanical failure. The objective of this study was to determine the long term effects of nucleus GAG depletion and to determine if altered discs demonstrate hallmark features of disc degeneration. We hypothesized that GAG will remain depleted 24 weeks post treatment, potentially decreasing to lower levels, and further that geometrical and mechanical changes consistent with degeneration will be observed.

Detection of Allergen Walnut Component in Food by an Improved Real-Time PCR Method

2009 ◽  
Vol 72 (11) ◽  
pp. 2433-2435 ◽  
Author(s):  
HAIYAN WANG ◽  
FEI YUAN ◽  
YAJUN WU ◽  
HAIRONG YANG ◽  
BAOLIANG XU ◽  
...  
Keyword(s):  
Detection Limit ◽  
Real Time ◽  
Real Time Pcr ◽  
Seed Storage Protein ◽  
Seed Storage ◽  
Plant Materials ◽  
Agricultural Plant ◽  
Negative Results ◽  
Pcr Method ◽  
Positive Results

A real-time PCR method aimed at the gene sequence of the walnut vicilin-like seed storage protein was established for the detection of the allergen walnut in food. The primers and probe were designed based on published methods. The method provided positive results for walnut and negative results for other tested agricultural plant materials including pecan. The intrinsic detection limit of the method was 0.00125 ng of walnut DNA, and the practical detection limit was 0.001% (wt/wt) walnut content in wheat; both of these values are lower than that of previously published methods. Therefore, this real-time PCR method is sufficiently specific and sensitive for the detection of walnut component in food.

Real-Time PCR Detection of Staphylococcus aureus in Milk and Meat Using New Primers Designed from the Heat Shock Protein Gene htrA Sequence

2007 ◽  
Vol 70 (12) ◽  
pp. 2855-2859 ◽  
Author(s):  
YU-CHENG CHIANG ◽  
CHIH-MING FAN ◽  
WAN-WEN LIAO ◽  
CHIEN-KU LIN ◽  
HAU-YANG TSEN
Keyword(s):  
Staphylococcus Aureus ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Real Time ◽  
Real Time Pcr ◽  
Protein Gene ◽  
Pcr Detection ◽  
Bacterial Strains ◽  
Heat Shock Protein Gene ◽  
Positive Results

Staphylococcus aureus may cause foodborne disease outbreaks and staphylococcal infections and is one of the major causes of mastitis. Rapid and reliable methods for detection of this microorganism in milk and other foods are needed. In this study, we designed a primer set from the sequence of the heat shock protein gene htrA, a gene coding for high-temperature-requirement A (HtrA) protein, and used it for real-time PCR detection of S. aureus isolates: 16 reference strains and 40 strains isolated from food-poisoning cases. All strains tested generated positive results. Bacterial strains other than S. aureus, including strains of other Staphylococcus species, did not produce positive results. When this primer set was used for the real-time PCR detection of S. aureus in milk and meat samples without the preenrichment step, samples with target cell numbers greater than 103 CFU/ml or CFU/g could be detected, indicating the potential quantitative ability of this real-time PCR assay. With a 10-h preenrichment step, however, a detection limit of 1 CFU/ml or CFU/g could be obtained.

scholarly journals Development of an Immunochromatographic Assay Kit Using Fluorescent Silica Nanoparticles for Rapid Diagnosis of Acanthamoeba Keratitis

2014 ◽  
Vol 53 (1) ◽  
pp. 273-277 ◽  
Author(s):  
Koji Toriyama ◽  
Takashi Suzuki ◽  
Tomoyuki Inoue ◽  
Hiroshi Eguchi ◽  
Saichi Hoshi ◽  
...  
Keyword(s):  
Silica Nanoparticles ◽  
Real Time ◽  
Real Time Pcr ◽  
High Sensitivity ◽  
Acanthamoeba Keratitis ◽  
Cross Reactivity ◽  
Immunochromatographic Assay ◽  
Content Type ◽  
Fluorescent Silica Nanoparticles ◽  
Positive Results

We developed an immunochromatographic assay kit that uses fluorescent silica nanoparticles bound to anti-Acanthamoebaantibodies (fluorescent immunochromatographic assay [FICGA]) and evaluated its efficacy for the detection ofAcanthamoebaand diagnosis ofAcanthamoebakeratitis (AK). The sensitivity of the FICGA kit was evaluated using samples ofAcanthamoebatrophozoites and cysts diluted to various concentrations. A conventional immunochromatographic assay kit with latex labels (LICGA) was also evaluated to determine its sensitivity in detectingAcanthamoebatrophozoites. To check for cross-reactivity, the FICGA was performed by using samples of other common causative pathogens of infectious keratitis, such asPseudomonas aeruginosa,Staphylococcus aureus,Staphylococcus epidermidis, andCandida albicans. Corneal scrapings from patients with suspected AK were tested with the FICGA kit to detect the presence ofAcanthamoeba, and the results were compared with those of real-time PCR. The FICGA kit detected organisms at concentrations as low as 5 trophozoites or 40 cysts per sample. There were no cross-reactivities with other pathogens. The FICGA was approximately 20 times more sensitive than the LICGA for the detection ofAcanthamoebatrophozoites. The FICGA kit yielded positive results for all 10 patients, which corresponded well with the real-time PCR results. The FICGA kit demonstrated high sensitivity for the detection ofAcanthamoebaand may be useful for the diagnosis of AK.

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