Phương pháp thử nghiệm độc học sử dụng hệ hô hấp của màng ty thể tim bò = Ecotoxicology tests based on the respiratory chain activity of beef heart mitochondrial particles.

2007 ◽  
Vol 10 (1) ◽  
Author(s):  
Nga Phi Lê ◽  
Thư Hiền Thị Nguyễn ◽  
Hoa Liên Thị Nguyễn ◽  
Hương Giang Thị Dương ◽  
Kháng Duy Đinh
Keyword(s):  
Respiratory Chain ◽  
Beef Heart ◽  
Respiratory Chain Activity

scholarly journals Ovarian Tumor Mitochondria Exhibit Abnormal Phenotypes and Blunted Associations with Biobehavioral Factors

2021 ◽  
Author(s):  
Snehal Bindra ◽  
Marlon McGill ◽  
Marina Triplett ◽  
Anisha Tyagi ◽  
Premal Thaker ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Respiratory Chain ◽  
Ovarian Tissue ◽  
Mitochondrial Respiratory Chain ◽  
Ovarian Tumors ◽  
Mitochondrial Content ◽  
Benign Tissue ◽  
Mitochondrial Respiratory Chain Activity ◽  
Respiratory Chain Activity ◽  
And Function

Abstract Tumor cells exhibit mitochondrial alterations and are also influenced by biobehavioral processes, but the intersection of biobehavioral factors and tumor mitochondria remains unexplored. Here we examined multiple biochemical and molecular markers of mitochondrial content and function in benign and cancerous ovarian tissue in parallel with exploratory analyses of biobehavioral factors. First, analysis of a publicly-available database (n=1,435) showed that gene expression of specific mitochondrial proteins in ovarian tumors is associated with survival. Quantifying multiple biochemical and molecular markers of mitochondrial content and function in 51 benign and 128 high-grade epithelial ovarian tumors revealed that compared to benign tissue, tumors exhibit 3.3-8.4-fold higher mitochondrial content and respiratory chain enzymatic activities (P<0.001) but similar mitochondrial DNA levels (-3.1%), documenting abnormal mitochondrial phenotypes in tumors. Mitochondrial respiratory chain activity was also associated with interleukin-6 (IL-6) levels in ascites. In benign tissue, negative biobehavioral factors were inversely correlated with mitochondrial content and respiratory chain activities, whereas positive biobehavioral factors tended to be positively correlated with mitochondrial measures, although effect sizes were small to medium (r=-0.43 to 0.47). In contrast, serous tumors showed less pronounced biobehavioral-mitochondrial correlations. These results document abnormal mitochondrial functional phenotypes in ovarian tumors and warrant further research on the link between biobehavioral factors and mitochondria in cancer.

Mitochondrial respiratory chain activity in an animal model of mania induced by ouabain

2011 ◽  
Vol 23 (3) ◽  
pp. 106-111 ◽  
Author(s):  
Tiago P. Freitas ◽  
Gislaine T. Rezin ◽  
Daiane B. Fraga ◽  
Morgana Moretti ◽  
Julia S. Vieira ◽  
...  
Keyword(s):  
Animal Model ◽  
Prefrontal Cortex ◽  
Respiratory Chain ◽  
Rebound Effect ◽  
Mitochondrial Respiratory Chain ◽  
Face Validity ◽  
Mitochondrial Respiratory Chain Activity ◽  
Respiratory Chain Activity ◽  
The Brain ◽  
Mitochondrial Respiratory

Objectives: Bipolar disorder (BD) is a mental illness associated with higher rates of suicide. The present study aims to investigate the brain mitochondrial respiratory chain activity in an animal model of mania induced by ouabain.Methods: Adult male Wistar rats received a single intracerebroventricular administration of ouabain (10−3 and 10−2 M) or vehicle. Locomotor activity was measured using the open field test. Mitochondrial respiratory chain activity was measured in the brain of rats 1 h and 7 days after ouabain administration.Results: Our results showed that spontaneous locomotion was increased 1 h and 7 days after ouabain administration. Complexes I, III and IV activities were increased in the prefrontal cortex, hippocampus and striatum immediately after the administration of ouabain, at the concentration of 10−3 and 10−2 M. Moreover, complex II activity was increased only in the prefrontal cortex at the concentration of 10−2 M. On the other hand, no significant alterations were observed in complex I activity 7 days after ouabain administration. However, an increase in complexes II, III and IV activities was observed only in the prefrontal cortex at the concentration of 10−2 M.Conclusion: Our findings suggest an increase in the activities of mitochondrial respiratory chain in this model of mania. A possible explanation is that these findings occur as a rebound effect trying to compensate for a decrease of ATP deprivation in BD. The present findings suggest that this model may present good face validity and a limitation in construct validity.

The inhibition of mammalian mitochondrial NADU oxidation by chloramphenicol and its isomers and analogues

1968 ◽  
Vol 46 (9) ◽  
pp. 1003-1008 ◽  
Author(s):  
K. B. Freeman ◽  
D. Haldar
Keyword(s):  
Cytochrome B ◽  
Respiratory Chain ◽  
Rat Liver ◽  
Nadh Dehydrogenase ◽  
Coenzyme Q ◽  
Liver Mitochondria ◽  
Heart Mitochondria ◽  
Rat Liver Mitochondria ◽  
Beef Heart ◽  
Beef Heart Mitochondria

Chloramphenicol and its isomers and analogues have been found to inhibit the oxidation of NADH, but not that of succinate, by beef heart mitochondria. They must therefore inhibit the NADH dehydrogenase segment of the respiratory chain. Chloramphenicol gave 50% inhibition at a concentration of 1 mM. The methylthio analogue of chloramphenicol inhibited NADH – coenzyme Q6 reductase but not NADH–ferricyanide reductase. Spectrophotometric observations suggest that these inhibitors act between NADH and flavin in coupled rat liver mitochondria and between flavin and cytochrome b in uncoupled beef heart mitochondria.

scholarly journals Linkage of E2F1 transcriptional network and cell proliferation with respiratory chain activity in breast cancer cells

2016 ◽  
Vol 107 (7) ◽  
pp. 963-971 ◽  
Author(s):  
Kazunori Mori ◽  
Tetsu Uchida ◽  
Motonori Fukumura ◽  
Shigetoshi Tamiya ◽  
Masato Higurashi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Proliferation ◽  
Cancer Cells ◽  
Respiratory Chain ◽  
Breast Cancer Cells ◽  
Transcriptional Network ◽  
Respiratory Chain Activity
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