scholarly journals Catalytic Active Hollow Fiber Membranes with an Enzyme-embedded MOF Coating

2020 ◽  
Author(s):  
Matthias Wessling
Keyword(s):  
Enzymatic Activity ◽  
Hollow Fiber ◽  
Enzyme Stability ◽  
Hollow Fiber Membrane ◽  
Continuous Process ◽  
Enzymatic Reaction ◽  
Convective Transport ◽  
Enzymatic Reactions ◽  
Active Centers ◽  
Selective Coating

Enzymatic reactions have the potential to enable a greener production of chemicals and pharmaceuticals. Enzyme stability, -recycling, and the implementation into a continuous process are vital points for the commercial success of enzymatic reactions. Recent publications revealed the suitability of Metal-Organic Frameworks (MOFs) as an enzyme immobilization matrix, with increased physiochemical stability. However, the separation of these enzyme MOFs and the additional transport resistances are still challenging. We report for the first time the in-situ biomineralization of enzymes into MOF structures via interfacial crystallization. This method proves to be effective for the selective coating of porous polymeric hollow fibers in a straightforward one-step process. We produced well adhering and stable enzyme embedded MOF layers with high enzymatic activity. The fusion of the enzymatic active layer and the hollow fiber membrane enables the continuous enzymatic reaction by the combination of permeation and reaction. The results show the successful improvement of the enzymatic activity due to the convective transport of educts and products to the enzymatic active centers during permeation.

EFFECT OF REACTION CONDITIONS ON THE SYNTHESIS OF CYCLODEXTRIN (CD) BY USING IMMOBILIZED ENZYME

2018 ◽  
Vol 80 (3) ◽  
Author(s):  
Suhaily Suhaimi ◽  
Rohaida Che Man ◽  
Natassha Jamil ◽  
Zatul Iffah Mohd Arshad ◽  
Shalyda Md Shaarani ◽  
...  
Keyword(s):  
Enzyme Immobilization ◽  
Hollow Fiber ◽  
Immobilized Enzyme ◽  
Hollow Fiber Membrane ◽  
Potato Starch ◽  
Enzymatic Reaction ◽  
Cyclodextrin Glucanotransferase ◽  
Fiber Membrane ◽  
Reaction Conditions ◽  
Agricultural Industries

The production of cyclodextrin (CD) over the years has been increasing due to the numerous applications in industries such as in food, cosmetic, pharmaceutical and agricultural industries. However, cyclodextrin glucanotransferase (CGTase) which involved in the enzymatic reaction on the production of CD is unstable and easily denatured at extreme conditions resulted in low CD production. Hence, the enzyme immobilization technique is introduced to overcome these problems and subsequently increase the production of CD. In the present study, the CGTase was immobilized on hollow fiber membrane to increase the production of CD during the reaction. The effect of reaction conditions (types of starch, concentration of starch, temperature and pH) of the immobilized enzyme on the production of CD were investigated. Among the three types of starch tested, the soluble potato starch was the most suitable substrate for the production of CD with 4.13 mg/mL. In addition, by using 3% (w/v) of the soluble potato starch, the production of CD was 5.22 mg/mL . The optimal reaction temperature and pH were found to be at 40°C and pH 6 with 5.21 mg/mL and 4.62 mg/ml of CD, respectively. The immobilized enzyme exhibited a 1.3-3-fold increase in CD production compared to the free enzyme. Therefore, the hollow fiber membrane is suitable to be used as a support for enzyme immobilization with the high production of CD.

scholarly journals EFFECT OF IMMOBILIZATION PARAMETERS ON THE IMMOBILIZATION OF CYCLODEXTRIN GLUCANOTRANFERASE ON HOLLOW FIBER MEMBRANE

2019 ◽  
Vol 82 (1) ◽  
Author(s):  
N. Jamil ◽  
R. C. Man ◽  
S. Suhaimi ◽  
S. M. Shaarani ◽  
Z. I. M. Arshad ◽  
...  
Keyword(s):  
Enzyme Immobilization ◽  
Hollow Fiber ◽  
Contact Time ◽  
Hollow Fiber Membrane ◽  
Enzymatic Reaction ◽  
Chemical Properties ◽  
Agitation Rate ◽  
Cyclodextrin Glucanotransferase ◽  
Fiber Membrane ◽  
High Production

Cyclodextrin (CD) is a non-reducing maltooligosaccharides which able to form inclusion complexes with many hydrophobic molecules, changing their physical and chemical properties. With these properties, CD has been discovered to have numerous applications in food industries, pharmaceutical, agricultural and environmental engineering. CD is produced by the enzymatic reaction between cyclodextrin glucanotransferase (CGTase) and starch. Various enzyme immobilization techniques such as adsorption, entrapment, encapsulation and cross-linking have been applied to improve the production of CD. Some of the immobilization parameters such as contact time, agitation rate and pH of the immobilization solution play a vital role in enzyme immobilization process, in order to achieve high production of CD. In the present study, the CGTase from Bacillus licheniformis was immobilized on polyvinylidene difluoride (PVDF) hollow fiber membrane via adsorption technique. The efficiency of enzyme immobilization appears to be affected by various factors (immobilization parameters) such as contact time, agitation rate and pH. Therefore, the effect of contact time (6-72 h), agitation rate (50-250 rpm) and pH (3-10) on the immobilization of CGTase on PVDF hollow fiber membrane were investigated in this study. The immobilized CGTase exhibited the highest immobilization yield of 69.37% under the conditions of 24 h contact time, 100 rpm and pH 7.0. Therefore, the influence of the immobilization parameters during the enzyme immobilization process is vital in order to achieve the high production of CD. Hence, high immobilization yield contributed to the high production of CD which in turn may be beneficial for the industrial purposes.

scholarly journals Impacts of Multilayer Hybrid Coating on PSF Hollow Fiber Membrane for Enhanced Gas Separation

Membranes ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 335
Author(s):  
Rosyiela Azwa Roslan ◽  
Woei Jye Lau ◽  
Gwo Sung Lai ◽  
Abdul Karim Zulhairun ◽  
Yin Fong Yeong ◽  
...  
Keyword(s):  
Gas Separation ◽  
Hollow Fiber ◽  
Coating Layer ◽  
Gas Permeability ◽  
Hollow Fiber Membrane ◽  
Membrane Surface ◽  
Selective Layer ◽  
Trade Off ◽  
Selective Coating ◽  
Gas Molecules

One of the most critical issues encountered by polymeric membranes for the gas separation process is the trade-off effect between gas permeability and selectivity. The aim of this work is to develop a simple yet effective coating technique to modify the surface properties of commonly used polysulfone (PSF) hollow fiber membranes to address the trade-off effect for CO2/CH4 and O2/N2 separation. In this study, multilayer coated PSF hollow fibers were fabricated by incorporating a graphene oxide (GO) nanosheet into the selective coating layer made of polyether block amide (Pebax). In order to prevent the penetration of Pebax coating solution into the membrane substrate, a gutter layer of polydimethylsiloxane (PDMS) was formed between the substrate and Pebax layer. The impacts of GO loadings (0.0–1.0 wt%) on the Pebax layer properties and the membrane performances were then investigated. XPS data clearly showed the existence of GO in the membrane selective layer, and the higher the amount of GO incorporated the greater the sp2 hybridization state of carbon detected. In terms of coating layer morphology, increasing the GO amount only affected the membrane surface roughness without altering the entire coating layer thickness. Our findings indicated that the addition of 0.8 wt% GO into the Pebax coating layer could produce the best performing multilayer coated membrane, showing 56.1% and 20.9% enhancements in the CO2/CH4 and O2/N2 gas pair selectivities, respectively, in comparison to the membrane without GO incorporation. The improvement is due to the increased tortuous path in the selective layer, which created a higher resistance to the larger gas molecules (CH4 and N2) compared to the smaller gas molecules (CO2 and O2). The best performing membrane also demonstrated a lower degree of plasticization and a very stable performance over the entire 50-h operation, recording CO2/CH4 and O2/N2 gas pair selectivities of 52.57 (CO2 permeance: 28.08 GPU) and 8.05 (O2 permeance: 5.32 GPU), respectively.

Simulation of Oxygen Permeability of Dual-phase Hollow Fiber Membrane

2012 ◽  
Vol 27 (9) ◽  
pp. 951-955
Author(s):  
Chun-Li YANG ◽  
Qi-Ming XU ◽  
Ming GONG ◽  
Wei LIU
Keyword(s):  
Hollow Fiber ◽  
Oxygen Permeability ◽  
Hollow Fiber Membrane ◽  
Dual Phase ◽  
Fiber Membrane

scholarly journals Kinetic Analysis of Lidocaine Elimination by Pig Liver Cells Cultured in 3D Multi-Compartment Hollow Fiber Membrane Network Perfusion Bioreactors

2021 ◽  
Vol 8 (8) ◽  
pp. 104
Author(s):  
Gerardo Catapano ◽  
Juliane K. Unger ◽  
Elisabetta M. Zanetti ◽  
Gionata Fragomeni ◽  
Jörg C. Gerlach
Keyword(s):  
Kinetic Analysis ◽  
Drug Screening ◽  
Hollow Fiber ◽  
Hollow Fiber Membrane ◽  
Liver Cells ◽  
Fiber Membrane ◽  
Bioreactor Design ◽  
Drug Adsorption ◽  
And Performance ◽  
Cells Cultured

Liver cells cultured in 3D bioreactors is an interesting option for temporary extracorporeal liver support in the treatment of acute liver failure and for animal models for preclinical drug screening. Bioreactor capacity to eliminate drugs is generally used for assessing cell metabolic competence in different bioreactors or to scale-up bioreactor design and performance for clinical or preclinical applications. However, drug adsorption and physical transport often disguise the intrinsic drug biotransformation kinetics and cell metabolic state. In this study, we characterized the intrinsic kinetics of lidocaine elimination and adsorption by porcine liver cells cultured in 3D four-compartment hollow fiber membrane network perfusion bioreactors. Models of lidocaine transport and biotransformation were used to extract intrinsic kinetic information from response to lidocaine bolus of bioreactor versus adhesion cultures. Different from 2D adhesion cultures, cells in the bioreactors are organized in liver-like aggregates. Adsorption on bioreactor constituents significantly affected lidocaine elimination and was effectively accounted for in kinetic analysis. Lidocaine elimination and cellular monoethylglicinexylidide biotransformation featured first-order kinetics with near-to-in vivo cell-specific capacity that was retained for times suitable for clinical assist and drug screening. Different from 2D cultures, cells in the 3D bioreactors challenged with lidocaine were exposed to close-to-physiological lidocaine and monoethylglicinexylidide concentration profiles. Kinetic analysis suggests bioreactor technology feasibility for preclinical drug screening and patient assist and that drug adsorption should be accounted for to assess cell state in different cultures and when laboratory bioreactor design and performance is scaled-up to clinical use or toxicological drug screening.

Sign in / Sign up

Export Citation Format

Share Document