Phytochemical screening and antimicrobial studies of afzelia africana and detarium microcarpum seeds

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Antimicrobial Activities ◽  
Gram Negative Bacteria ◽  
Physiological Effects ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Antimicrobial Studies ◽  
Detarium Microcarpum ◽  
Phytochemical Constituents ◽  
Afzelia Africana ◽  
Seed Extracts

The aim of this study was to probe the phytochemical constituents and the antimicrobial activities of Afzelia africana and Detarium microcarpum seed endosperms. The results obtained from the phytochemical screening indicated that tannins, flavonoids, fatty acids, phenol, steroids, saponins and alkaloids were present. The seed extracts were tested against eight pathogenic organisms comprising of two Gram positive and two Gram negative bacteria; two fungi and two viruses using Agar and Disc diffusion methods. The plant extracts exhibited antimicrobial activities against all the tested organisms. This investigation therefore, suggests the incorporation of Afzelia africana and Detarium microcarpum seeds into human diets as they are rich in medicinal agents that could trigger great physiological effects. It also authenticates their use as soup thickeners in eastern Nigeria and in the production of snacks.

scholarly journals PHYTOCHEMICAL AND ANTIMICROBIAL STUDIES ON GARCINIA LATTISSIMA MIQ. FRUIT EXTRACT

2017 ◽  
Vol 10 (7) ◽  
pp. 230 ◽  
Author(s):  
Neneng Sitisilfi Ambarwati ◽  
Berna Elya ◽  
Amarila Malik ◽  
Muhammad Hanafi
Keyword(s):  
Sustainable Use ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Fruit Extract ◽  
Traditional System ◽  
Antimicrobial Studies ◽  
Methanolic Extracts ◽  
Standard Procedures ◽  
Phytochemical Constituents ◽  
Fruit Part

Objective: The present investigation was aimed to explore the phytoconstituents present in the fruit part of Garcinia lattissima Miq. and their antimicrobial efficacy.Methods: The preliminary phytochemical constituents were qualitatively analyzed using the standard procedures described in Materia Medica Indonesia. Antimicrobial screening was performed using disc diffusion and dilution methods.Results: Phytochemical screening of different extracts of G. lattissima Miq. fruits revealed the presence of tannins, saponins, flavonoids, and alkaloids, and the results are shown in Table 1. The ethyl acetate and methanolic extracts of G. lattissima Miq. fruits showed antimicrobial activity, and the n-hexane extract failed to prove the inhibition against the selected pathogens.Conclusion: The results of the phytochemical and bio-efficacy study revealed most valuable information and also support the continued sustainable use of G. lattissima Miq. fruits in the traditional system of medicine.

scholarly journals Synthesis, Characterization and Antimicrobial Activities of Co (III) and Fe (III) Complexes of Imino-N-heterocyclic Carbenes Ligands

2021 ◽  
pp. 59-67
Author(s):  
Samaila Abubakar ◽  
Musa Muktari ◽  
Rejoice Atiko
Keyword(s):  
Escherichia Coli ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Heterocyclic Carbenes ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Gram Negative ◽  
E Coli ◽  
Gram Positive Bacteria

The synthesis and antimicrobial application of Co (III) and Fe (III) complexes of imine functionalized N-heterocyclic carbene (Imino-NHC) ligands is reported. The ligand precursors 1-(2-[(hydroxyl-benzylidene)-amino]-ethyl)-3-R-3H-imidazol-1-ium bromide where R = pyridyl (1a) and benzyl (1b) have been reported in our previous work. The in-situ generated ligands of 1a and 1b have been successfully coordinated to CoBr2 and [FeI(Cp)(CO)2] leading to the isolation of air-stable N^C^N^O four coordinate Co(III)  complex 2 and a six-coordinate Fe(III) complex 3. The synthesised complexes were both found to be NMR inactive hence were characterize using FTIR and LRMS. The complexes were screened for antimicrobial activities against four gram-negative bacteria Escherichia Coli (E-coli), Shigella, Klebsiella pneumoniae (K. Pneumoniae) and Salmonella typhi (S. typhi) and a gram positive bacteria Staphylocossus aureus (S. aureus). The antimicrobial test was conducted using disc diffusion methods and based on the concentrations of 100, 200, 300, 400 and 500 µg/ mL, significant activities were recorded for both cobalt and the iron complexes.

scholarly journals Analysis of a “3-(Naphthalen-1-ylimino)indolin-2-one” Compound and Its Antimicrobial Assessment Using Lipid-Based Self-Nanoemulsifying Formulations

Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 15
Author(s):  
Saeed Ali Syed ◽  
Ahmed Bari ◽  
Mohammed S. Aldughaim ◽  
Md Abdur Rashid ◽  
Mohammad Hossain Shariare ◽  
...  
Keyword(s):  
Biological Activities ◽  
Wide Spectrum ◽  
Parent Drug ◽  
Antimicrobial Activities ◽  
Growth Effect ◽  
Gram Negative Bacteria ◽  
Standard Samples ◽  
Gram Negative ◽  
Antimicrobial Studies ◽  
Liquid Chromatographic

In recent years, indole derivatives have acquired conspicuous significance due to their wide spectrum of biological activities—antibacterial, antiviral, and anticonvulsant. This compound is derived from naturally grown plants. Therefore, synthesis of a novel “3-(Naphthalen-1-ylimino)indolin-2-one” compound (2) and its analysis using UPLC systems along with antimicrobial assessment was the aim of the current study. Isatin was used as a parent drug for synthesizing compound (2). Liquid Chromatographic analysis was performed using a C18 BEH column (1.7 μm 2.1 × 50 mm) by UPLC systems. Degradation studies were carried out to see whether acid, base, thermal, and oxidizing agents had any impact on the synthesized molecule in stress conditions (100 °C). A lipid-based self-nanoemulsifying formulation was developed and selectivity, specificity, recovery, accuracy, and precision were measured as part of the UPLC system’s validation process. Antimicrobial studies were conducted using gram-positive and gram-negative bacteria. The standard samples were run with a concentration range of 5.0–100.0 μg/mL using the isocratic mobile phase comprising of methanol/water (70/30 %v/v) at 234 nm; good linearity (R2 = 0.9998) was found. The lower limits of detection (LOD) and quantitation (LOQ) of the method were found to be 0.81 μg/mL and 2.5 μg/mL, respectively. The coefficients of variation were found to be less than 2%. The antimicrobial study suggests that compound (2) has a substantial growth effect against gram-negative bacteria. It was successfully synthesized and applied to measure the concentrations in lipid-based dosage form, along with potent antimicrobial activities.

scholarly journals Synthesis, spectroscopic characterization and antimicrobial studies of mercury(II) complexes of thiolates

2009 ◽  
Vol 23 (1) ◽  
pp. 45-50 ◽  
Author(s):  
Ghulam Rabbani ◽  
Anvarhusein A. Isab ◽  
Abdul Rahman Al-Arfaj ◽  
Saeed Ahmad ◽  
Muhammad Saleem ◽  
...  
Keyword(s):  
Inhibitory Concentration ◽  
Spectroscopic Characterization ◽  
Antimicrobial Activities ◽  
Moderate Activity ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
E Coli ◽  
Antimicrobial Studies ◽  
Wide Range ◽  
C Nmr

Mercury(II) complexes of thiolates having the general formula [Hg(RS)Cl2]−and [Hg(RS)2] have been prepared and characterized by IR and NMR (1H and13C) spectroscopy (RSH = 2-aminoethanethiol hydrochloride (Aet), cysteine (Cys), thiosalicylic acid (Ts) and 2-mercaptonicotinic acid (Mnt)). The spectral data suggests that the coordination of thiolates to mercury(II) occurs through the sulfur as indicated by the absence of S–H vibrations in IR and significant downfield shifts in the C–S resonance in13C NMR. However, in Mnt complexes, coordination through both sulfur and nitrogen is indicated. Antimicrobial activities of the complexes were evaluated by minimum inhibitory concentration and the results showed that the complexes exhibited a wide range of activities against gram-negative bacteria (E. coli, P. aeruginosa), while moderate activity was observed against a mold,P. citrinum.

scholarly journals SCREENING ANTIMICROBIAL POTENTIAL FOR MALAYSIAN ORIGINATED TAMARINDUS INDICA ETHANOLIC LEAVES EXTRACT

2018 ◽  
Vol 11 (3) ◽  
pp. 361 ◽  
Author(s):  
Sridevi Chigurupati ◽  
Eric Wong Kwang Yiik ◽  
Jahidul Islam Mohammad ◽  
Shantini Vijayabalan ◽  
Kesavanarayanan Krishnan Selvarajan ◽  
...  
Keyword(s):  
Soxhlet Extraction ◽  
Extraction Methods ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Tamarindus Indica ◽  
Gram Negative ◽  
Antimicrobial Potential ◽  
Antimicrobial Studies ◽  
Ethanolic Extraction ◽  
Phytochemical Constituents

 Objective: The aim of the research is to study the antimicrobial potential of Tamarindus indica leaves extract (Malaysian origin).Methods: T. indica leaves extraction was carried out by maceration (TIME) and Soxhlet extraction methods (TISE). The phytochemical test was conducted for the confirmation of various phytoconstituents present in the leaves extract. Antimicrobial assays were carried out by minimum bactericidal concentration (MBC), minimum inhibitory concentration (MIC), and agar well diffusion method against ATCC bacterial strains.Results: Ethanolic extraction of T. indica leaves by maceration method showed better yield (70.38%) compared to Soxhlet extraction method (60.55%). The qualitative phytochemical test on TISE and TIME were confirmed the presence of alkaloids, flavonoids, phenols, mucilage, tannins, steroids, proteins, and carbohydrates. TIME was selected for antimicrobial studies, and it was found to have MBC value of 500 μg/mL and MIC value of 250 μg/mL among various test bacteria, and it was also found to exhibit good zone of inhibition against Gram-negative bacteria such as Escherichia coli and Neisseria gonorrhoeae. Conclusion: These findings deduced that T. indica leaves have various phytochemical constituents which could be responsible for their natural antibacterial activity especially against Gram-negative bacteria.

scholarly journals Phytochemical Screening and Antimicrobial Activities of Seed Extracts From Sandoricum Koetjape

2013 ◽  
Vol 4 (1) ◽  
pp. 104-104
Author(s):  
Saripah Salbiah Syed Abd. Azziz ◽  
Hasimah Alimon ◽  
Asmaniza Abdullah Sani ◽  
Norhayati Daud ◽  
Nor Nafizah Mohd Noor
Keyword(s):  
Antimicrobial Activities ◽  
Phytochemical Screening ◽  
Sandoricum Koetjape ◽  
Seed Extracts

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTI-MICROBIAL ACTIVITY OF ETHANOLIC EXTRACTS OF FRUITS OF ANNONA RETICULATA AGAINST STANDARD PATHOGENIC STRAINS

2018 ◽  
Vol 10 (4) ◽  
pp. 59
Author(s):  
Sujata Paul ◽  
Biswajit Dash ◽  
Aditya Jyoti Bora ◽  
Bikash Gupta
Keyword(s):  
Microbial Activity ◽  
Ethanolic Extract ◽  
Soxhlet Extraction ◽  
Phytochemical Analysis ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
Phytochemical Constituents ◽  
Annona Reticulata

Objective: To evaluate the anti-microbial activity of ethanolic extract of fruits of Annona reticulata (family-Annonaceae) in conjugation with phytochemical analysis.Methods: The ethanolic extract of fruits of Annona reticulata (family-Annonaceae) was prepared by Soxhlet extraction and analysed for phytochemical constituents using standard methods. The anti-microbial activity of the plant extract was examined against bacterial strains and fungal strains using disc diffusion method.Results: The present investigation shows the phytochemical analysis, anti-microbial activity of the ethanolic extract of the plant Annona reticulata. Various phytochemical analyses revealed the presence of alkaloids, saponin, flavonoids, carbohydrates, glycosides, steroids, proteins and amino acids and tannins. The anti-microbial activity of the ethanolic extract of the plant showed significant result against all the of the test organisms.Conclusion: The present study concluded that ethanolic extract of fruits of the Annona reticulata contain high presence of phytochemicals. The ethanolic extract of the plant was found to possess promising anti-microbial activity when compared with the standards.

scholarly journals Investigating the Phytochemicals and Antimicrobial Properties of Three Sedge (Cyperaceae) Species

2014 ◽  
Vol 6 (3) ◽  
pp. 276-281 ◽  
Author(s):  
Tiwalade Adeyemi ADENIYI ◽  
Peter A. ADEONIPEKUN ◽  
Elizabeth A. OMOTAYO
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Cyperus Rotundus ◽  
New Drugs ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Isolation And Purification ◽  
Phytochemical Constituents

In order to evaluate the medicinal value of notorious sedge weeds, three species:Cyperus esculentus, Cyperus rotundus and Mariscus alternifolius were investigated for their phytochemical constituents and antimicrobial properties. Preliminary qualitative phytochemical constituents and in vitro antimicrobial activities were evaluated against four fungi species: Aspergillus niger, Aspergillus fumigatus, Penicillium chrysogenum and Candida albicans, and three bacteria species: Escherichia coli,Salmonella typhi and Staphylococcus aureus. Two solvents, water and ethanol, were used to produce the extracts and were screened for their antimicrobial activity. Antimicrobial activity evaluation of the extracts against pathogens was carried out at 100 mg/ml concentration by Disc Diffusion method for fungi, Disc Diffusion and Agar Well Diffusion methods for bacteria. Observed activities were related to standard antibiotics, antifungal and antibacterial, which served as controls. Phytochemically, the plant extracts showed the presence of carbohydrates, flavonoids, ketose sugars, steroids, reducing sugars and tannins. The ethanolic extract of C. rotundus exhibited the highest activity against A. niger, E. coli and S. aureus. No extract was active against C. albicans. From these findings, it was concluded that C. rotundus is a potential source of bioactive compounds for new drugs upon isolation and purification for treating infections caused by these pathogens.

In-vitro Antioxidant and Antibacterial Activity of Methanolic Extract of Shorea robusta Gaertn. F. Resin

2016 ◽  
Vol 6 (4) ◽  
pp. 68
Author(s):  
Sushma Vashisht ◽  
Manish Pal Singh ◽  
Viney Chawla
Keyword(s):  
Antibacterial Activity ◽  
Methanolic Extract ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Gram Positive ◽  
Gram Negative ◽  
Shorea Robusta ◽  
Dose Dependent ◽  
Resin Extract

The methanolic extract of the resin of Shorea robusta was subjected to investigate its antioxidant and antibacterial properties its utility in free radical mediated diseases including diabetic, cardiovascular, cancer etc. The methanol extract of the resin was tested for antioxidant activity using scavenging activity of DPPH (1,1-diphenyl-2-picrylhydrazil) radical method, reducing power by FeCl3 and antibacterial activity against gram positive and gram negative bacteria using disc diffusion method. The phytochemical screening considered the presence of triterpenoids, tannins and flavoniods. Overall, the plant extract is a source of natural antioxidants which might be helpful in preventing the progress of various oxidative stress mediated diseases including aging. The half inhibition concentration (IC50) of resin extract of Shorea robusta and ascorbic acid were 35.60 µg/ml and 31.91 µg/ml respectively. The resin extract exhibit a significant dose dependent inhibition of DPPH activity. Antibacterial activity was observed against gram positive and gram negative bacteria in dose dependent manner.Key Words: Shorea robusta, antioxidant, antibacterial, Disc-diffusion, DPPH.

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