scholarly journals Phosphate Nutrition and Defoliation Effects on Growth and Root Physiology of Alfalfa

2018 ◽  
Author(s):  
Synan AbuQamar ◽  
Suzanne M. Cunningham ◽  
Jeffrey J. Volenec
Keyword(s):  
Shoot Growth ◽  
Transcript Abundance ◽  
Forage Yield ◽  
P Availability ◽  
Mrna Levels ◽  
P Deficiency ◽  
High Affinity ◽  
Greenhouse Study ◽  
Medicago Sativa L ◽  
Shoot Removal

Phosphorus (P) deficiency reduces forage yield and stand persistence of alfalfa (Medicago sativa L.). The objective of this study was to determine the influence of P nutritionand defoliation on alfalfa shoot growth, root carbohydrate and protein metabolism, and steady-state mRNA levels for high-affinity P transporters. In a greenhouse study, P-deprived plants were provided with 0, 0.25, 2, and 6 mM P beginning 7 d before shoot removal. Plants were sampled immediately (day -7) on days -5, -2, 0 (day of shootremoval), and on days 1, 2, 6, and 9 post-shoot removal. Addition of P to P-deficient plants stimulated growth of shoots but not roots. Taproot bark sugar concentrations were reduced significantly in cut plants at any rate of P, whereas only the 6 mM P treatment reduced taproot wood sugar concentrations in uncut plants. There was a significant defoliation-induced decline in both wood and bark sugar and amino-acid concentration that was enhanced at high P rates. Low P reduced utilization of starch and protein reserves in taproots. Transcripts for a high-affinity P transporter were not detected in any root or shoot tissue assayed, irrespective of defoliation or P treatment. The uncertain relationship between P availability and P-transporter transcript abundance in our greenhouse-grown plants requires additional investigation.

scholarly journals Phosphate nutrition effects on growth, phosphate transporter transcript levels and physiology of alfalfa cells

2018 ◽  
Author(s):  
Synan AbuQamar ◽  
Thomas G. Sors ◽  
Suzanne M. Cunningham ◽  
Jeffrey J. Volenec
Keyword(s):  
Medicago Sativa ◽  
Cultured Cells ◽  
Phosphorus Deficiency ◽  
Forage Yield ◽  
Phosphate Transporter ◽  
Amino Acid Sequence Similarity ◽  
P Nutrition ◽  
Transcript Levels ◽  
High Affinity ◽  
Medicago Sativa L

Phosphorus deficiency reduces forage yield and stand persistence of alfalfa (Medicago sativa L.). Our objectives were to isolate and characterize a high-affinity phosphate-transporter (P-transporter) from alfalfa roots (Medicago sativa L.); determine how phosphorus (P) nutrition impacts P-uptake, growth, and carbohydrate and protein metabolism of alfalfa cells; and learn how expression of the P-transporter is influenced by P nutrition. An 1087-base pair (bp) sequence was isolated using RT-PCR that possessed high nucleotide and amino acid sequence similarity to high-affinity P-transporters. Cultured cells were sampled at 3-day intervals for 9 days while growing in media containing P concentrations ranging from 0 to 10 mM. Media P concentrations declined rapidly in all P treatments by day 6. Low media P concentrations (0, 0.1 and 0.5 mM) reduced cell growth rates compared to higher media P levels (2.5, 5 and 10 mM). Suspension cell cultures supplied 0.5, 2.5, 5, and 10 mM P had lower starch concentrations by day 3 compared to cells cultured in media containing 0 and 0.1 mM P. Steady-state transcript levels for the high-affinity P- transporter were high in P-deprived cells, but declined within 1 day when cells were provided 10 mM P.

Sex-specifically responsive strategies to phosphorus availability combined with different soil nitrogen forms in dioecious Populus cathayana

2021 ◽  
Author(s):  
Xiucheng Liu ◽  
Yuting Wang ◽  
Shuangri Liu ◽  
Miao Liu
Keyword(s):  
Root Length ◽  
Root Length Density ◽  
Specific Root Length ◽  
P Uptake ◽  
Root Tip ◽  
P Availability ◽  
P Deficiency ◽  
Populus Cathayana ◽  
Leaf P ◽  
P Supply

Abstract Aims Phosphorus (P) availability and efficiency are especially important for plant growth and productivity. However, the sex-specific P acquisition and utilization strategies of dioecious plant species under different N forms are not clear. Methods This study investigated the responsive mechanisms of dioecious Populus cathayana females and males based on P uptake and allocation to soil P supply under N deficiency, nitrate (NO3 −) and ammonium (NH4 +) supply. Important Findings Females had a greater biomass, root length density (RLD), specific root length (SRL) and shoot P concentration than males under normal P availability with two N supplies. NH4 + supply led to higher total root length, RLD and SRL but lower root tip number than NO3 − supply under normal P supply. Under P deficiency, males showed a smaller root system but greater photosynthetic P availability and higher leaf P remobilization, exhibiting a better capacity to adaptation to P-deficiency than females. Under P deficiency, NO3 − supply increased leaf photosynthesis and PUE but reduced RLD and SRL in females while males had higher leaf P redistribution and photosynthetic PUE than NH4 + supply. Females had a better potentiality to cope with P deficiency under NO3 − supply than NH4 + supply; the contrary was true for males. These results suggest that females may devote to increase in P uptake and shoot P allocation under normal P availability, especially under NO3 − supply, while males adopt more efficient resource use and P remobilization to maximum their tolerance to P-deficiency.

scholarly journals Irrigation Timing as a Practice of Effective Weed Management in Established Alfalfa (Medicago sativa L.) Crop

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 550
Author(s):  
Panagiotis Kanatas ◽  
Ioannis Gazoulis ◽  
Ilias Travlos
Keyword(s):  
Medicago Sativa ◽  
Weed Management ◽  
Cultural Practices ◽  
Block Design ◽  
Chenopodium Album ◽  
Stand Density ◽  
Climatic Conditions ◽  
Amaranthus Retroflexus ◽  
Forage Yield ◽  
Medicago Sativa L

Irrigation is an agronomic practice of major importance in alfalfa (Medicago sativa L), especially in the semiarid environments of Southern Europe. Field experimentation was conducted in Western Greece (2016–2018) to evaluate the effects of irrigation timing on weed presence, alfalfa yield performance, and forage quality. In a randomized complete block design (four replications), two cultivars (“Ypati 84” and “Hyliki”) were the main plots, while three irrigation timings were the subplots (split-plot). The irrigation timings were IT-1, IT-2, and IT-3, denoting irrigation 1 week before harvest, 1 week after harvest, and 2 weeks after harvest, respectively. IT-1 reduced Solanum nigrum L. density by 54% and 79% as compared to IT-3 and IT-2, respectively. Chenopodium album L. density was the highest under IT-2. IT-3 resulted in 41% lower Amaranthus retroflexus L. density in comparison to IT-2, while the lowest values were observed under IT-1. Stand density and stems·plant−1 varied between years (p ≤ 0.05). Mass·stem−1 and alfalfa forage yield were affected by the irrigation timings (p ≤ 0.001). Total weed density and forage yield were negatively correlated in both the second (R2 = 87.013%) and the fourth (R2 = 82.691%) harvests. IT-1 and IT-3 increased forage yield, leaf per stem ratio, and crude protein as compared to IT-2. Further research is required to utilize the use of cultural practices for weed management in perennial forages under different soil and climatic conditions.

scholarly journals Granulosal and thecal expression of bone morphogenetic protein- and activin-binding protein mRNA transcripts during bovine follicle development and factors modulating their expression in vitro

Reproduction ◽  
2011 ◽  
Vol 142 (4) ◽  
pp. 581-591 ◽  
Author(s):  
Claire Glister ◽  
Leanne Satchell ◽  
Phil G Knight
Keyword(s):  
Bone Morphogenetic Proteins ◽  
Transforming Growth Factor ◽  
Ex Vivo ◽  
Mrna Level ◽  
Transcript Abundance ◽  
Transforming Growth Factor Β ◽  
Follicle Development ◽  
Mrna Levels ◽  
Theca Interna

Evidence supports local roles for transforming growth factor β superfamily members including activins and bone morphogenetic proteins (BMP) in follicle development. Access of these ligands to signalling receptors is likely modulated by extracellular binding proteins (BP). In this study, we comparedex vivoexpression of four BPs (chordin, gremlin, noggin and follistatin) in granulosal (GC) and theca interna (TC) compartments of developing bovine antral follicles (1–18 mm). Effects of FSH and IGF on BMP and BP expression by cultured GC, and effects of LH and BMPs on BP expression by cultured TC were also examined. Follicular expression of all four BP transcripts was higher in GC than TC compartments (P<0.001) a finding confirmed by immunohistochemistry. Follicle category affected (P<0.01) gremlin and follistatin mRNA abundance, with a significant cell-type×follicle category interaction for chordin, follistatin and noggin. Noggin transcript abundance was lower (P<0.05) in GC of large ‘E-active’ than ‘E-inactive’ follicles while follistatin mRNA level was higher (P<0.01). FSH enhanced CYP19, FSHR, INHBA and follistatin by GC without affecting BMP or BMP–BP expression. IGF increased CYP19 and follistatin, reduced BMP4, noggin and gremlin but did not affect chordin orFSHRmRNA levels. LH increased TC androgen secretion but had no effect on BMP or BP expression. BMPs uniformly suppressed TC androgen production whilst increasing chordin, noggin and gremlin mRNA levels up to 20-fold (P<0.01). These findings support the hypothesis that extracellular BP, mostly from GC, contribute to the regulation of intrafollicular BMP/activin signalling. Enhancement of thecal BP expression by BMP implies an autoregulatory feedback role to prevent excessive signalling.

scholarly journals Morphological and Metabolite Responses of Potatoes under Various Phosphorus Levels and Their Amelioration by Plant Growth-Promoting Rhizobacteria

2021 ◽  
Vol 22 (10) ◽  
pp. 5162
Author(s):  
Leangsrun Chea ◽  
Birgit Pfeiffer ◽  
Dominik Schneider ◽  
Rolf Daniel ◽  
Elke Pawelzik ◽  
...  
Keyword(s):  
Amino Acids ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Shoot Biomass ◽  
Root Surface Area ◽  
Limiting Factor ◽  
P Availability ◽  
P Deficiency ◽  
Plant Growth Promoting ◽  
Growth Promoting

Low phosphorus (P) availability is a major limiting factor for potatoes. P fertilizer is applied to enhance P availability; however, it may become toxic when plants accumulate at high concentrations. Therefore, it is necessary to gain more knowledge of the morphological and biochemical processes associated with P deficiency and toxicity for potatoes, as well as to explore an alternative approach to ameliorate the P deficiency condition. A comprehensive study was conducted (I) to assess plant morphology, mineral allocation, and metabolites of potatoes in response to P deficiency and toxicity; and (II) to evaluate the potency of plant growth-promoting rhizobacteria (PGPR) in improving plant biomass, P uptake, and metabolites at low P levels. The results revealed a reduction in plant height and biomass 60–80% under P deficiency compared to P optimum. P deficiency and toxicity conditions also altered the mineral concentration and allocation in plants due to nutrient imbalance. The stress induced by both P deficiency and toxicity was evident from an accumulation of proline and total free amino acids in young leaves and roots. Furthermore, root metabolite profiling revealed that P deficiency reduced sugars by 50–80% and organic acids by 20–90%, but increased amino acids by 1.5–14.8 times. However, the effect of P toxicity on metabolic changes in roots was less pronounced. Under P deficiency, PGPR significantly improved the root and shoot biomass, total root length, and root surface area by 32–45%. This finding suggests the potency of PGPR inoculation to increase potato plant tolerance under P deficiency.

scholarly journals Silicon reduces aluminum content in tissues and ameliorates its toxic effects on potato plant growth

2016 ◽  
Vol 46 (3) ◽  
pp. 506-512 ◽  
Author(s):  
Athos Odin Severo Dorneles ◽  
Aline Soares Pereira ◽  
Liana Verônica Rossato ◽  
Gessieli Possebom ◽  
Victória Martini Sasso ◽  
...  
Keyword(s):  
Chemical Element ◽  
Shoot Growth ◽  
Aluminum Content ◽  
Solanum Tuberosum L ◽  
Toxic Effects ◽  
Al Content ◽  
Potato Plants ◽  
High Affinity ◽  
Root And Shoot Growth ◽  
Tolerant Genotype

ABSTRACT: Aluminum (Al) is highly toxic to plants, causing stress and inhibiting growth and silicon (Si) is considered beneficial for plants. This chemical element has a high affinity with Al. The aim of this study was to investigate the potential of Si to mitigate the toxic effects of Al on potato ( Solanum tuberosum L.) plants and assess whether this behavior is different among genotypes with differing degrees of sensitivity to Al. Potato plants of the genotypes SMIJ319-7 (Al-sensitive) and SMIF212-3 (Al-tolerant) were grown for fourteen days in nutrient solution (without P and pH 4.5±0.1) under exposure to combinations of Al (0 and 1.85mM) and Si (0, 0.5 and 1.0mM). After this period, shoot and roots of the two genotypes were collected to determine Al content in tissues and assess morphological parameters of root and shoot growth. Roots of both genotypes accumulated more Al than shoots and the Al-tolerant genotype accumulated more Al than the sensitive one, both in roots and in shoot. Furthermore, the presence of 0.5 and 1.0mM Si together with Al reduced the Al content in shoot in both genotypes and in roots of the Al-tolerant genotype, respectively. Si ameliorated the toxic effects of Al with regard to number of root branches and leaf number in both potato genotypes. Si has the potential to mitigate the toxic effects of Al in potato plants regardless of Al sensitivity.

scholarly journals Cultivar-Dependent Responses in Plant Growth, Leaf Physiology, Phosphorus Use Efficiency, and Tuber Quality of Potatoes Under Limited Phosphorus Availability Conditions

2021 ◽  
Vol 12 ◽  
Author(s):  
Leangsrun Chea ◽  
Ana Meijide ◽  
Catharina Meinen ◽  
Elke Pawelzik ◽  
Marcel Naumann
Keyword(s):  
Tuber Yield ◽  
Plant Biomass ◽  
P Uptake ◽  
Tuber Quality ◽  
P Availability ◽  
Plant Tolerance ◽  
P Deficiency ◽  
Leaf Physiology ◽  
Use Efficiency

The limited availability of phosphorus (P) in soils causes a major constraint in the productivity of potatoes, which requires increased knowledge of plant adaptation responses in this condition. In this study, six potato cultivars, namely, Agria, Lady Claire, Milva, Lilly, Sieglinde, and Verdi, were assessed for their responses on plant growth, leaf physiology, P use efficiency (PUE), and tuber quality with three P levels (Plow, Pmed, and Phigh). The results reveal a significant variation in the cultivars in response to different P availabilities. P-efficient cultivars, Agria, Milva, and Lilly, possessed substantial plant biomass, tuber yield, and high P uptake efficiency (PUpE) under low P supply conditions. The P-inefficient cultivars, Lady Claire, Sieglinde, and Verdi, could not produce tubers under P deprivation conditions, as well as the ability to efficiently uptake P under low-level conditions, but they were efficient in P uptake under high soil P conditions. Improved PUpE is important for plant tolerance with limited P availability, which results in the efficient use of the applied P. At the leaf level, increased accumulations of nitrate, sulfate, sucrose, and proline are necessary for a plant to acclimate to P deficiency-induced stress and to mobilize leaf inorganic phosphate to increase internal PUE and photosynthesis. The reduction in plant biomass and tuber yield under P-deficient conditions could be caused by reduced CO2 assimilation. Furthermore, P deficiency significantly reduced tuber yield, dry matter, and starch concentration in Agria, Milva, and Lilly. However, contents of tuber protein, sugars, and minerals, as well as antioxidant capacity, were enhanced under these conditions in these cultivars. These results highlight the important traits contributing to potato plant tolerance under P-deficient conditions and indicate an opportunity to improve the P efficiency and tuber quality of potatoes under deficient conditions using more efficient cultivars. Future research to evaluate molecular mechanisms related to P and sucrose translocation, and minimize tuber yield reduction under limited P availability conditions is necessary.

scholarly journals Contribution of Arbuscular Mycorrhizal Fungi, Phosphate–Solubilizing Bacteria, and Silicon to P Uptake by Plant

2021 ◽  
Vol 12 ◽  
Author(s):  
Hassan Etesami ◽  
Byoung Ryong Jeong ◽  
Bernard R. Glick
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Crop Production ◽  
Current Knowledge ◽  
Arbuscular Mycorrhizal ◽  
P Availability ◽  
Phosphate Solubilizing Bacteria ◽  
P Deficiency ◽  
Phosphate Solubilizing ◽  
P Fertilizers

Phosphorus (P) availability is usually low in soils around the globe. Most soils have a deficiency of available P; if they are not fertilized, they will not be able to satisfy the P requirement of plants. P fertilization is generally recommended to manage soil P deficiency; however, the low efficacy of P fertilizers in acidic and in calcareous soils restricts P availability. Moreover, the overuse of P fertilizers is a cause of significant environmental concerns. However, the use of arbuscular mycorrhizal fungi (AMF), phosphate–solubilizing bacteria (PSB), and the addition of silicon (Si) are effective and economical ways to improve the availability and efficacy of P. In this review the contributions of Si, PSB, and AMF in improving the P availability is discussed. Based on what is known about them, the combined strategy of using Si along with AMF and PSB may be highly useful in improving the P availability and as a result, its uptake by plants compared to using either of them alone. A better understanding how the two microorganism groups and Si interact is crucial to preserving soil fertility and improving the economic and environmental sustainability of crop production in P deficient soils. This review summarizes and discusses the current knowledge concerning the interactions among AMF, PSB, and Si in enhancing P availability and its uptake by plants in sustainable agriculture.

scholarly journals Water and phosphorus uptake by upland rice root systems unraveled under multiple scenarios: linking a 3D soil-root model and data

2020 ◽  
Author(s):  
Trung Hieu Mai ◽  
Pieterjan De Bauw ◽  
Andrea Schnepf ◽  
Roel Merckx ◽  
Erik Smolders ◽  
...  
Keyword(s):  
Upland Rice ◽  
Phosphorus Uptake ◽  
Root Systems ◽  
Multiscale Model ◽  
P Uptake ◽  
Water Dynamics ◽  
Small Scale ◽  
P Availability ◽  
P Deficiency ◽  
Plant P Uptake

AbstractBackground and aimsUpland rice is often grown where water and phosphorus (P) are limited and these two factors interact on P bioavailability. To better understand this interaction, mechanistic models representing small-scale nutrient gradients and water dynamics in the rhizosphere of full-grown root systems are needed.MethodsRice was grown in large columns using a P-deficient soil at three different P supplies in the topsoil (deficient, suboptimal, non-limiting) in combination with two water regimes (field capacity versus drying periods). Root architectural parameters and P uptake were determined. Using a multiscale model of water and nutrient uptake, in-silico experiments were conducted by mimicking similar P and water treatments. First, 3D root systems were reconstructed by calibrating an architecure model with observed phenological root data, such as nodal root number, lateral types, interbranch distance, root diameters, and root biomass allocation along depth. Secondly, the multiscale model was informed with these 3D root architectures and the actual transpiration rates. Finally, water and P uptake were simulated.Key resultsThe plant P uptake increased over threefold by increasing P and water supply, and drying periods reduced P uptake at high but not at low P supply. Root architecture was significantly affected by the treatments. Without calibration, simulation results adequately predicted P uptake, including the different effects of drying periods on P uptake at different P levels. However, P uptake was underestimated under P deficiency, a process likely related to an underestimated affinity of P uptake transporters in the roots. Both types of laterals (i.e. S- and L-type) are shown to be highly important for both water and P uptake, and the relative contribution of each type depend on both soil P availability and water dynamics. Key drivers in P uptake are growing root tips and the distribution of laterals.ConclusionsThis model-data integration demonstrates how multiple co-occurring single root phene responses to environmental stressors contribute to the development of a more efficient root system. Further model improvements such as the use of Michaelis constants from buffered systems and the inclusion of mycorrhizal infections and exudates are proposed.

Sign in / Sign up

Export Citation Format

Share Document