scholarly journals Interactions of ericoid mycorrhizal fungi and root pathogens in Rhododendron: In vitro tests with plantlets in sterile liquid culture

Plant Root ◽  
2013 ◽  
Vol 7 (0) ◽  
pp. 33-48 ◽  
Author(s):  
Gisela Grunewaldt-Stöcker ◽  
Christiane von den Berg ◽  
Johanna Knopp ◽  
Henning von Alten
Keyword(s):  
Mycorrhizal Fungi ◽  
Liquid Culture ◽  
Ericoid Mycorrhizal Fungi ◽  
Root Pathogens

Molecular diversity of ericoid mycorrhizal fungi

2000 ◽  
Vol 77 (11) ◽  
pp. 1580-1594
Author(s):  
Marcia Monreal ◽  
S M Berch ◽  
Mary Berbee
Keyword(s):  
Sequence Analysis ◽  
Dna Sequences ◽  
Mycorrhizal Fungi ◽  
Molecular Data ◽  
Internal Transcribed Spacers ◽  
Field Site ◽  
Specific Primers ◽  
Single Root ◽  
Ericoid Mycorrhizal Fungi

Using restriction fragment length polymorphism (RFLP) patterns from two ribosomal internal transcribed spacers (ITS) and DNA sequences from ITS2, we characterized ericoid mycorrhizal fungal isolates from culture collections.With a synoptic key to RFLP patterns, we divided 34 mycorrhizal or root-associated isolates into 16 groups. RFLP patterns were identical when fungal specific primers were used to amplify DNA from pure fungal cultures and in vitro mycorrhizae. Sequence analysis clustered 23 of 24 mycorrhizal isolates into two larger groups: the Oidiodendron group and the Hymenoscyphus group. The Oidiodendron group included genetically uniform, conidiating fungi. The Hymenoscyphus group encompassed more diversity and included other discomycetes (Leotiales) as well as sterile, unidentifiable mycorrhizal isolates from four RFLP groups. Results from our field site on Vancouver Island, British Columbia, Canada, suggest that several ericoid mycorrhizal fungi can coexist in a single root of Gaultheria shallon Pursh and that our molecular data base is not yet complete. From sixty 3-mm root sections, we cultured four genetically different fungi that formed mycorrhizae in resynthesis experiments and sequence analysis showed that one of these differed from all previously known ericoid mycorrhizal fungi.

scholarly journals In Vitro Colonization of Micropropagated Pieris floribunda by Ericoid Mycorrhizae. II. Effects on Acclimatization and Growth

HortScience ◽  
2001 ◽  
Vol 36 (2) ◽  
pp. 357-359 ◽  
Author(s):  
Mark C. Starrett ◽  
Frank A. Blazich ◽  
Steven R. Shafer ◽  
Larry F. Grand
Keyword(s):  
Mycorrhizal Fungi ◽  
Hymenoscyphus Ericae ◽  
Ericoid Mycorrhizal Fungi ◽  
Ericoid Mycorrhizae ◽  
In Vitro Inoculation

Inoculation of microshoots of Pieris floribunda (Pursh ex Sims) Benth. and Hook. (mountain andromeda) with isolates of Hymenoscyphus ericae (Read) Korf and Kernan ericoid mycorrhizal fungi stimulated growth during 1 month in vitro. However, no benefits were apparent after 3 months in a greenhouse. Acclimatization of plantlets of P. floribunda to greenhouse conditions following in vitro inoculation improved survival (42% vs. 16% for controls). The protocol reported herein is similar to procedures utilized currently for micropropagation of various ericaceous species and has potential to improve plantlet survival during acclimatization.

Isolation and identification of hydroxamate siderophores of ericoid mycorrhizal fungi

BioMetals ◽  
1992 ◽  
Vol 5 (1) ◽  
pp. 51-56 ◽  
Author(s):  
Kurt Haselwandter ◽  
Barbara Dobernigg ◽  
Werner Beck ◽  
G�nther Jung ◽  
Alexander Cansier ◽  
...  
Keyword(s):  
Mycorrhizal Fungi ◽  
Isolation And Identification ◽  
Ericoid Mycorrhizal Fungi ◽  
Hydroxamate Siderophores

On the cultivation of spir. pallida on liquid culture media

1926 ◽  
Vol 22 (1) ◽  
pp. 19-22
Author(s):  
V. M. Aristovskiy ◽  
R. R. Gel'ttser
Keyword(s):  
Liquid Culture ◽  
Culture Media ◽  
Nutrient Media ◽  
Reliable Methods

In recent years, great strides have been made on the cultivation of spirochetes, and if relatively simple and completely reliable methods of growing them in vitro on both solid and liquid nutrient media have been developed for a number of pathogenic and non-pathogenic spirochetes, then on the issue of With the cultivation of the pale spirochete, we are moving forward very slowly, and the successes achieved recently in this area must be recognized as very modest.

scholarly journals Artificial Cultivation System for Gastrodia spp. and Identification of Associated Mycorrhizal Fungi

2017 ◽  
Vol 9 (4) ◽  
pp. 27 ◽  
Author(s):  
Chie Shimaoka ◽  
Hirokazu Fukunaga ◽  
Seishu Inagaki ◽  
Shinichiro Sawa
Keyword(s):  
Mycorrhizal Fungi ◽  
Flowering Plants ◽  
Ex Situ ◽  
Natural Conditions ◽  
Cultivation System ◽  
Horticultural Plants ◽  
The Relationship ◽  
Single Tuber ◽  
Artificial Cultivation

The Orchidaceae are the largest and most diverse family of flowering plants on earth, and include some of the most important horticultural plants. While mycoheterotrophic orchids belonging to the genus Gastrodia are known to be provided with carbon through mycorrhizal fungi, the relationship between the plants and fungi is poorly understood. Furthermore, it is challenging to cultivate Gastrodia spp. in vitro. In this study, we present an efficient method for germinating Gastrodia pubilabiata (Gp), Gastrodia nipponica (Gn), and Gastrodia confusa (Gc) plants in vitro, which results in the production of a protocorm and tuber, as under natural conditions. The Gp and Gc plants produced flowers 126 and 124 days after germination, respectively, and set seed under our artificial conditions. In addition, Gp plants flowered up to three times a year from a single tuber. Using our artificial cultivation system, we identified some of the mycorrhizal fungi associated with these plants. Gastrodia spp. appear to obtain carbon from many kinds of mycorrhizal fungi. Our artificial cultivation method is a rapid and efficient means of growing Gastrodia spp. In addition to having applications in research and commercial nurseries, this method could be used to conserve Gastrodia spp. in ex situ, many of which are endangered.

Activity of myxin against Ceratocystis ulmi

1969 ◽  
Vol 15 (1) ◽  
pp. 133-135 ◽  
Author(s):  
E. A. Peterson
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Liquid Culture ◽  
Inhibitory Concentration ◽  
In Vitro Tests ◽  
Strong Inhibition ◽  
Solid Media ◽  
Highly Sensitive ◽  
Paper Disc ◽  
Disc Assay

Eight strains of Ceratocystis ulmi originating from different locations and host species were found to be highly sensitive to the antibiotic myxin in in vitro tests. By paper disc assay, amounts as low as 0.5–1.0 μg caused strong inhibition of the fungus on solid media. The minimum inhibitory concentration in liquid culture was 0.2 μg/ml and levels of antibiotic above this concentration proved to be fungicidal.

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