scholarly journals Species diversity in Penicillium and Talaromyces from herbivore dung, and the proposal of two new genera of penicillium-like fungi in Aspergillaceae

2020 ◽  
Vol 5 (1) ◽  
pp. 39-76 ◽  
Author(s):  
M. Guevara-Suarez ◽  
D. García ◽  
J.F. Cano-Lira ◽  
J. Guarro ◽  
J. Gené
Keyword(s):  
Species Diversity ◽  
Rna Polymerase Ii ◽  
New Taxa ◽  
Species Recognition ◽  
Sequencing Analysis ◽  
New Genera ◽  
Coprophilous Fungi ◽  
Rdna Its ◽  
Genealogical Concordance ◽  
High Level

Coprophilous fungi are saprotrophic organisms that show great diversity, mainly on herbivore dung. The physico-chemical characteristics of this peculiar substrate combined with the high level of fungal adaptation to different environmental conditions offer the perfect setting for discovering new taxa. This study focused on the species diversity of penicillium-like fungi isolated mainly from herbivore dung collected at different Spanish locations. From 130 samples, a total of 104 isolates were obtained, and 48 species were identified. Preliminary identifications were based on morphology and partial β-tubulin (tub2) gene sequences. Putative new taxa were characterized by a multi-gene sequencing analysis testing the tub2, the internal transcribed spacer rDNA (ITS), calmodulin (cmdA), and RNA polymerase II second largest subunit (rpb2) genes, and a detailed phenotypic study. Using this polyphasic approach and following the genealogical concordance phylogenetic species recognition (GCPSR) method, we propose the new genera Penicillago (for Penicillium nodositatum) and Pseudopenicillium (for Penicillium megasporum and P. giganteum) in the family Aspergillaceae, and 11 new species, including seven Penicillium, three Talaromyces and one Pseudopenicillium. A lectotype and epitype are designed for Penicillium nodositatum. Our results show that the species diversity of penicillium-like fungi on herbivore dung has not been widely studied and that this substrate seems to be a good reservoir of interesting Eurotialean fungi.

scholarly journals Species Diversity in Penicillium and Acaulium From Herbivore Dung in China, and Description of Acaulium stericum Sp. Nov.

2021 ◽  
Author(s):  
Lei Su ◽  
Hua Zhu ◽  
Peilin Sun ◽  
Xue Li ◽  
Bochao Yang ◽  
...  
Keyword(s):  
Species Diversity ◽  
Internal Transcribed Spacer ◽  
Food Industry ◽  
Gene Sequencing ◽  
Sequencing Analysis ◽  
Good Source ◽  
Rdna Its ◽  
Herbivorous Animal ◽  
Internal Transcribed Spacer Rdna ◽  
Β Tubulin

Abstract Penicillium and Acaulium species are common in the fresh of herbivore dung and can produce abundant secondary metabolism, which play important roles as decomposers of organic materials, food industry, and enzyme factories. Besides, the well-characterized diversity of dung fungi offers accessible systems for dissecting the function of fungi in gut and for exploring potential to produce high cellulases in herbivorous animal. During a survey of intestinal fungi from herbivorous animal in China, more than 400 were isolated, 38 belonging to Penicillium and 4 belonging to Acaulium were obtained from 12 healthy animals including marmot and chinchilla and selected for detailed study. Putative taxa were characterized by a multi-gene sequencing analysis testing the partial β-tubulin (TUB), the internal transcribed spacer rDNA (ITS), calmodulin (CAM), and RPB2, and a detailed phenotypic study. Penicillium strains were identified as six sections, 12 known species. In addition, four Acaulium isolates were identified as Acaulium album and Acaulium stericum sp. nov. based on morphology and phylogeny of multi-gene sequences. This study shows that the species diversity of Penicillium on herbivore dung has not been widely studied and that seems to be a good source of offers opportunities for discovery of new cellulases from microbial communities.

scholarly journals Diversity within Aspergillus niger Clade and Description of a New Species: Aspergillus vinaceus sp. nov.

2020 ◽  
Vol 6 (4) ◽  
pp. 371
Author(s):  
Josué J. da Silva ◽  
Beatriz T. Iamanaka ◽  
Larissa S. Ferranti ◽  
Fernanda P. Massi ◽  
Marta H. Taniwaki ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Secondary Metabolites ◽  
Rna Polymerase Ii ◽  
Random Amplified Polymorphic Dna ◽  
Species Recognition ◽  
Sensu Stricto ◽  
Phylogenetic Species ◽  
Gene Sequences ◽  
Genealogical Concordance ◽  
Sclerotium Production

Diversity of species within Aspergillus niger clade, currently represented by A. niger sensu stricto and A. welwitshiae, was investigated combining three-locus gene sequences, Random Amplified Polymorphic DNA, secondary metabolites profile and morphology. Firstly, approximately 700 accessions belonging to this clade were investigated using calmodulin gene sequences. Based on these sequences, eight haplotypes were clearly identified as A. niger (n = 247) and 17 as A. welwitschiae (n = 403). However, calmodulin sequences did not provide definitive species identities for six haplotypes. To elucidate the taxonomic position of these haplotypes, two other loci, part of the beta-tubulin gene and part of the RNA polymerase II gene, were sequenced and used to perform an analysis of Genealogical Concordance Phylogenetic Species Recognition. This analysis enabled the recognition of two new phylogenetic species. One of the new phylogenetic species showed morphological and chemical distinguishable features in comparison to the known species A. welwitschiae and A. niger. This species is illustrated and described as Aspergillus vinaceus sp. nov. In contrast to A. niger and A. welwitschiae, A. vinaceus strains produced asperazine, but none of them were found to produce ochratoxin A and/or fumonisins. Sclerotium production on laboratory media, which does not occur in strains of A. niger and A. welwitschiae, and strictly sclerotium-associated secondary metabolites (14-Epi-hydroxy-10,23-dihydro-24,25-dehydroaflavinine; 10,23-Dihydro-24,25-dehydroaflavinine; 10,23-Dihydro-24,25-dehydro-21-oxo-aflavinine) were found in A. vinaceus. The strain type of A. vinaceus sp. nov. is ITAL 47,456 (T) (=IBT 35556).

scholarly journals Phylogeny and species delimitation of Strobilomyces (Boletaceae), with an emphasis on the Asian species

2020 ◽  
Vol 44 (1) ◽  
pp. 113-139
Author(s):  
L.H. Han ◽  
G. Wu ◽  
E. Horak ◽  
R.E. Halling ◽  
J. Xu ◽  
...  
Keyword(s):  
Rna Polymerase Ii ◽  
Molecular Phylogenetics ◽  
Species Recognition ◽  
Elongation Factor ◽  
Distribution Patterns ◽  
Distinct Species ◽  
Morphological Characters ◽  
Phylogenetic Species ◽  
Type Specimens ◽  
Genealogical Concordance

Strobilomyces is broadly distributed geographically and serves an important ecological function. However, it has been difficult to delimit species within the genus, primarily due to developmental variations and phenotypic plasticity. To elucidate phylogenetic relationships among species within the genus and to understand its species diversity, especially in Asia, materials of the genus collected from five continents (Africa, Asia, Australia, Europe, and North/Central America) were investigated. The phylogeny of Strobilomyces was reconstructed based on nucleotide sequences of four genes coding for: the largest and the second largest subunits of the RNA polymerase II (RPB1 and RPB2); the translation elongation factor subunit 1-α (TEF1); and the mitochondrial cytochrome oxidase subunit 3 (COX3). The combined results based on molecular phylogenetics, morphological characters, host tree associations, and geographical distribution patterns support a new classification consisting of two sections, sect. Strobilomyces and sect. Echinati. Using the genealogical concordance phylogenetic species recognition (GCPSR) approach, at least 33 phylogenetic species in Asia can be delimited, all of which are supported by morphological features, and five phylogenetic species remain to be described. The mountainous region of Southwest China is especially special, containing at least 21 species and likely represents a centre of diversification. We further compared our specimens with the type specimens of 25 species of Strobilomyces. Our comparisons suggest that, there are a total of 31 distinct species, while S. sanmingensis, S. verruculosus, S. subnigricans, and S. zangii/S. areolatus, are synonyms of S. mirandus, S. giganteus, S. alpinus and S. seminudus, respectively. Eight new species, namely, S. albidus, S. anthracinus, S. calidus, S. cingulatus, S. densisquamosus, S. douformis, S. microreticulatus and S. pinophilus, are described. A dichotomous key to the Asian Strobilomyces species is provided.

Eriophyidae mites from Cupania trees (Sapindaceae) from the Atlantic Forest in Brazil: three new genera and five new species

2017 ◽  
Vol 22 (3) ◽  
pp. 341
Author(s):  
Mercia Elias Duarte ◽  
Edmilson Santos Silva ◽  
Denise Navia
Keyword(s):  
New Species ◽  
Atlantic Forest ◽  
New Taxa ◽  
New Genus ◽  
New Genera ◽  
Native Trees ◽  
Two New Species ◽  
One New Species

Eight new taxa of Eriophyidae mites associated with native trees in the Cupania genus—C. oblongifolia Mart. and C. impressinervia Acev (Sapindaceae)—from the Atlantic Forest in Brazil, are described and illustrated. They include two new genera and two new species of Nothopodinae, Colopodacini (Setibia domatiagena   gen. nov., sp. nov. and Aricolopodos alagoensis gen. nov., sp. nov.), one new genus and two new species of Cecidophyinae, Colomerini (Euryslobos keronidos gen. nov., sp. nov. and Gammaphytoptus cupanius sp. nov.), and one new species of Phyllocoptinae, Tegonotini (Shevtchenkella caboata sp. nov.).

New Australian Erotylinae with notes on Dacnini (Coleoptera: Cucujoidea: Erotylidae)

Zootaxa ◽  
2021 ◽  
Vol 4948 (3) ◽  
pp. 363-380
Author(s):  
PAUL E. SKELLEY ◽  
RICHARD A. B. LESCHEN ◽  
ZHENHUA LIU
Keyword(s):  
New Species ◽  
New Taxa ◽  
New Genus ◽  
New Genera ◽  
New Genus And Species

In preparation for upcoming studies, several new taxa of Australian Erotylinae (Coleoptera: Erotylidae) need to be described to make names available. New genera and species in Dacnini and Megalodacnini are described. Echinothallis banderbearella, new genus and species (Dacnini); Microdacne, new genus, with four new species (Dacnini), M. gloriousa, M. lamingtonia, M. nardia, M. styxia; and Episcaphula (Tropidoscaphula) megalodacnoides, new species (Megalodacnini). Variation of female terminalia and other characters in several genera are discussed regarding the monophyly and generic diversity of Dacnini. 

Floral diversity, phytogeography, and climatic amelioration during the Early Carboniferous (Dinantian)

Paleobiology ◽  
1985 ◽  
Vol 11 (3) ◽  
pp. 293-309 ◽  
Author(s):  
Anne Raymond
Keyword(s):  
Plant Diversity ◽  
Early Carboniferous ◽  
High Latitudes ◽  
New Genera ◽  
Generic Level ◽  
The North ◽  
Floral Diversity ◽  
High Level ◽  
In The Beginning

Phytogeographic analysis of three Early Carboniferous intervals (Tournaisian–early Visean, Visean, and late Visean–early Namurian A) indicates a high level of phytogeographic differentiation in the beginning of the Early Carboniferous that decreases toward the end of this period. Climatic amelioration (warmer or wetter conditions) in the north middle and high latitudes, caused by the collision of Laurussia and Gondwana at the end of the Early Carboniferous, may be responsible for this decrease in phytogeographic provinciality. Toward the end of the Early Carboniferous, a large number of equatorial genera expand their ranges northward, and the average generic diversity of assemblages in the north high latitudes (Siberia) also rises. Both support the hypothesis of climatic amelioration. Northward migration of equatorial forms and the appearance of new genera endemic to Siberia both contributed to the rise in Siberian diversity. Although this trend is not statistically significant, additional evidence of diversity increase in northern high latitudes tied to climatic amelioration comes from the northernmost limit of diverse (≥ 10 genera) assemblages, which rises from 20°N at the beginning to 55°N at the end of the Early Carboniferous. Global plant diversity assessed at the generic level remained constant during the Early Carboniferous. The increase in Siberian diversity was offset by a decrease in equatorial diversity, perhaps due to the loss of pronounced latitudinal climatic gradients between north-middle and equatorial latitudes.

Combined multi-gene backbone tree for the genus Coniochaeta with two new species from Uzbekistan

Phytotaxa ◽  
2018 ◽  
Vol 336 (1) ◽  
pp. 43 ◽  
Author(s):  
MILAN C. SAMARAKOON ◽  
YUSUFJON GAFFOROV ◽  
NINGGUO LIU ◽  
SAJEEWA S. N. MAHARACHCHIKUMBURA ◽  
JAYARAMA D. BHAT ◽  
...  
Keyword(s):  
New Species ◽  
Sequence Data ◽  
Species Recognition ◽  
Phylogenetic Species ◽  
Phylogenetic Studies ◽  
Genealogical Concordance ◽  
Two New Species ◽  
Backbone Tree

The genus Coniochaeta is an important ascomycete because its members live in diversified habitats and nutritional modes. In this study, two new species, C. acaciae and C. coluteae, are introduced from dead branches of Acacia sp. and Colutea paulsenii Freyn (both Fabaceae) respectively from Uzbekistan, based on morphological and phylogenetic studies. Analyses of combined ITS and LSU sequence data with Genealogical Concordance Phylogenetic Species Recognition (GCPSR) and comparison of similar taxa, provide evidences for placement of these new species in Coniochaeta, as distinct lineages.

scholarly journals Aldosterone reprograms promoter methylation to regulate αENaC transcription in the collecting cuct

2013 ◽  
Vol 305 (7) ◽  
pp. F1006-F1013 ◽  
Author(s):  
Zhiyuan Yu ◽  
Qun Kong ◽  
Bruce C. Kone
Keyword(s):  
Promoter Methylation ◽  
Dna Methyltransferase ◽  
Cpg Island ◽  
Collecting Duct ◽  
Epigenetic Mechanism ◽  
Sequencing Analysis ◽  
Control Of Gene Expression ◽  
Bisulfite Treatment ◽  
High Level ◽  
Interfering Rna

Aldosterone increases tubular Na+ absorption largely by increasing α-epithelial Na+ channel (αENaC) transcription in collecting duct principal cells. How aldosterone reprograms basal αENaC transcription to high-level activity in the collecting duct is incompletely understood. Promoter methylation, a covalent but reversible epigenetic process, has been implicated in the control of gene expression in health and disease. We investigated the role of promoter methylation/demethylation in the epigenetic control of basal and aldosterone-stimulated αENaC transcription in mIMCD3 collecting duct cells. Bisulfite treatment and sequencing analysis after treatment of the cells with the DNA methyltransferase (DNMT) inhibitor 5-aza-2′-deoxycytidine (5-Aza-CdR) identified clusters of methylated cytosines in a CpG island near the transcription start site of the αENaC promoter. 5-Aza-CdR treatment or small interfering RNA-mediated knockdown of DNMT3b or methyl-CpG-binding domain protein (MBD)-4 derepressed basal αENaC transcription, indicating that promoter methylation suppresses basal αENaC transcription. Aldosterone triggered a time-dependent decrease in 5mC and DNMT3b and a concurrent enrichment in 5-hydroxymethylcytosine (5hmC) and ten-eleven translocation (Tet)2 at the αENaC promoter, consistent with active demethylation. 5-Aza-CdR mimicked aldosterone by enhancing Sp1 binding to the αENaC promoter. We conclude that DNMT3b- and MBD4-dependent methylation of the αENaC promoter limits basal αENaC transcription, in part by limiting Sp1 binding and trans-activation. Aldosterone stimulates the dispersal of DNMT3b and recruitment of Tet2 to demethylate the αENaC promoter to induce αENaC transcription. These results disclose a novel epigenetic mechanism for the control of basal and aldosterone-induced αENaC transcription that adds to previously described epigenetic controls exerted by histone modifications.

scholarly journals Evaluation of the Relationship among Biogenic Amines, Nitrite and Microbial Diversity in Fermented Mustard

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6173
Author(s):  
Yangyang Yu ◽  
Lu Li ◽  
Yujuan Xu ◽  
Kejing An ◽  
Qiao Shi ◽  
...  
Keyword(s):  
Biogenic Amines ◽  
High Throughput Sequencing ◽  
Starter Cultures ◽  
Sequencing Analysis ◽  
Maximum Residue Limit ◽  
Safety Standard ◽  
Bacteria And Fungi ◽  
High Level ◽  
Degradation Properties ◽  
The Relationship

Biogenic amines (BAs) and nitrites are both considered harmful compounds for customer health, and are closely correlated with the microorganisms in fermented mustard (FM). In this study, BAs and nitrite contents in fifteen FM samples from different brands were analyzed. The concentrations of cadaverine in one sample and of histamine in one sample were above the toxic level. Moreover, five FM samples contained a high level of nitrite, exceeding the maximum residue limit (20 mg/kg) suggested by the National Food Safety Standard. Then, this study investigated bacterial and fungal communities by high-throughput sequencing analysis. Firmicutes and Basidiomycota were identified as the major bacteria and fungi phylum, respectively. The correlations among microorganisms, BAs and nitrite were analyzed. Typtamine showed a positive correlation with Lactobacillus and Pseudomonas. Cadaverine and nitrite is positively correlated with Leuconostoc. Furthermore, thirteen strains were selected from the samples to evaluate the accumulation and degradation properties of their BAs and nitrite. The results indicated that the Lactobacillus isolates, including L. plantarum GZ-2 and L. brevis SC-2, can significantly reduce BAs and nitrite in FM model experiments. This study not only assessed the contents of BAs and nitrite in FM samples, but also provided potential starter cultures for BAs and nitrite control in the FM products industry.

scholarly journals Vegetation analysis and plant diversity in Pinus Jantho Forest (PJF) nature reserve, Aceh Besar

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
HILMINA ITAWAMERNI ◽  
SAIDA RASNOVI ◽  
ZUMAIDAR ZUMAIDAR
Keyword(s):  
Species Diversity ◽  
Plant Species ◽  
Nature Reserve ◽  
Diversity Index ◽  
Systematic Sampling ◽  
Vegetation Analysis ◽  
High Level ◽  
Piper Aduncum ◽  
Important Value Index ◽  
Pinus Merkusii

Pinus Jantho Forest (PJF) Nature Reserve has unique characteristics and distinctive ecosystem that plays an important role in the preservation of germplasm and the protection of natural resources. The sustainability of the PJF Nature Reserve is strongly determined by vegetation of its constituents. Therefore, an effort is needed to help its management properly, one of which is by knowing the diversity of plants that make up the community in PJF Nature Reserve. The purpose of this research is to analyze the composition and diversity of plant species in PJF Nature Reserve. Data collection and sampling were done using multiple square method. The plots were laid out by systematic sampling. PJF Nature Reserve composed of 111 species from 46 family. The seedling and understorey have the highest number individuals (1028 in total). Then there are 240 individuals at sapling level, 108 individuals at pole, and 72 individuals for tree.  The Euphorbiaceae family has the largest number of species (11 species). The highest Important Value Index (IVI) for seedlings and understorey, sapling and pole, and tree respectively were Chloranthus elatior (11.09%), Piper aduncum (21.90% and 19.32%), and Pinus merkusii (25.52%). The Diversity Index indicates a high level of plant species diversity (3.25-3.63).

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