scholarly journals In vitro effects of some drugs on human erythrocyte glutathione reductase

2011 ◽  
Vol 27 (1) ◽  
pp. 18-23 ◽  
Author(s):  
Muhammet Karaman ◽  
Ebru Akkemik ◽  
Harun Budak ◽  
Mehmet Ciftci
Keyword(s):  
Glutathione Reductase ◽  
Human Erythrocyte ◽  
In Vitro Effects

Deciphering of The Effect of Chemotherapeutic Agents on Human Glutathione S-Transferase Enzyme and MCF-7 Cell Line

2020 ◽  
Vol 27 (9) ◽  
pp. 888-894
Author(s):  
Havva Aybek ◽  
Yusuf Temel ◽  
Barzan Mirza Ahmed ◽  
Can Ali Ağca ◽  
Mehmet Çiftci
Keyword(s):  
Cell Line ◽  
Human Erythrocyte ◽  
Chemotherapeutic Agents ◽  
Cellular Viability ◽  
Ammonium Sulfate Precipitation ◽  
Glutathione S Transferase ◽  
In Vitro Effects ◽  
First Time ◽  
Mcf 7

Background: Cancer is the disease that causes the most death after cardiovascular diseases all over the world these days. Breast cancer is the most common type of cancer among women and ranks the second among cancer-related deaths after lung cancer. Chemotherapeutics act by killing cancer cells, preventing their spread and slowing their growth. Recent studies focus on the effects of chemotherapeutics on cancer cells and new chemotherapy approaches that targeting enzymes that catalyze important metabolic reactions in the cell. Objective: The aim of this study was to investigate the effects of chemotherapeutic agents, Tamoxifen and 5-FU, on MCF-7 cell line and human erythrocyte GST, an important enzyme of intracellular antioxidant metabolism. Methods: In this study, it was investigated that the effect of chemotherapeutic agents, Tamoxifen and 5-FU, on MCF-7 breast cancer cell line and performed ROS analyzes. In addition, it was purified glutathione S-transferase (GST), one of the important enzymes of intracellular antioxidant mechanism, from human erythrocytes by using ammonium sulfate precipitation and glutathione agarose affinity chromatography, and investigated in vitro effects of chemotherapeutic agents, 5 - FU and Tamoxifen, on the activity of this enzyme for the first time. Results: it was determined that Tamoxifen and 5-FU inhibited cellular viability and 5-FU increased intracellular levels of ROS, whereas Tamoxifen reduced intracellular levels of ROS. In addition, human erythrocyte GST enzyme with 16.2 EU/mg specific activity was purified 265.97-fold with a yield of 35% using ammonium sulfate precipitation and glutathione agarose affinity chromatography. The purity of the enzyme was checked by the SDS-PAGE method. In vitro effects of chemotherapeutics, 5-FU and Tamoxifen, on GST activity purified from human erythrocytes were investigated. The results showed that 5-FU increased the activity of GST in the concentration range of 77 to 1155 μM and that Tamoxifen increased the activity of GST in the concentration range of 0.54 to 2.70 μM. Conclusion: In this study, the effects of tamoxifen and 5-FU chemotherapeutic agents on both MCF-7 cell line and human GST enzyme were examined together for the first time. Our study showed that chemotherapeutic agents (5-FU and Tamoxifen) inhibited cellular viability and Tamoxifen reduced intracellular levels of ROS whereas 5-FU increased intracellular levels of ROS. In addition, 5-FU and Tamoxifen were found to increase the activity of GST enzyme purified from the human erythrocyte.

In vitro inhibition of human erythrocyte glutathione reductase by some new organic nitrates

2009 ◽  
Vol 19 (13) ◽  
pp. 3661-3663 ◽  
Author(s):  
Murat Şentürk ◽  
Oktay Talaz ◽  
Deniz Ekinci ◽  
Hüseyin Çavdar ◽  
Ömer İrfan Küfrevioğlu
Keyword(s):  
Glutathione Reductase ◽  
Human Erythrocyte ◽  
Organic Nitrates ◽  
In Vitro Inhibition

In vitro effects of the anti-Alzheimer drug memantine on the human erythrocyte membrane and molecular models

2017 ◽  
Vol 483 (1) ◽  
pp. 528-533 ◽  
Author(s):  
Pablo Zambrano ◽  
Mario Suwalsky ◽  
Fernando Villena ◽  
Malgorzata Jemiola-Rzeminska ◽  
Kazimierz Strzalka
Keyword(s):  
Erythrocyte Membrane ◽  
Human Erythrocyte ◽  
Molecular Models ◽  
Human Erythrocyte Membrane ◽  
In Vitro Effects

scholarly journals In vitro effects of compounds isolated from Sideritis brevibracteata on bovine kidney cortex glutathione reductase.

2011 ◽  
Vol 58 (4) ◽  
Author(s):  
Berivan Tandogan ◽  
Ayşegül Güvenç ◽  
İhsan Çalış ◽  
Nuriye Nuray Ulusu
Keyword(s):  
Glutathione Reductase ◽  
Reduced Glutathione ◽  
Kidney Cortex ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Bovine Kidney ◽  
Aerial Parts ◽  
In Vitro Effects

Glutathione reductase (GR, E.C 1.6.4.2) is a flavoprotein that catalyzes NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH). The aim of this study was to investigate in vitro effects of phenolic compounds isolated from Sideritis brevibracteata on bovine kidney GR. The Sideritis species are widely found in nature and commonly used as medicinal plants. 7-O-glycosides of 8-OH-flavones (hypolaetin, isoscutellarein and 3'-hydroxy-4'-O-methylisoscutellarein) were isolated from aerial parts of Sideritis brevibracteata. These compounds inhibited bovine kidney cortex GR in a concentration-dependent manner. Kinetic characterization of the inhibition was also performed.

In Vitro Effects of Isoorientin, Forsythoside B, and Verbascoside on Bovine Kidney Cortex Glutathione Reductase

2013 ◽  
Vol 45 (9) ◽  
pp. 574-579 ◽  
Author(s):  
Berivan Tandogan ◽  
Ayşegül Guvenc ◽  
Ihsan Calis ◽  
Nuriye Nuray Ulusu
Keyword(s):  
Glutathione Reductase ◽  
Kidney Cortex ◽  
Bovine Kidney ◽  
In Vitro Effects
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