Role of Surfactants on the Activity of Acid Phosphatase Incorporated in Reverse Micelles

2000 ◽  
Vol 18 (5) ◽  
pp. 331-341 ◽  
Author(s):  
Krishnakant Soni ◽  
Claudia Shah ◽  
Datta Madamwar
Keyword(s):  
Acid Phosphatase ◽  
Reverse Micelles

Studies of Vegetative Plant Tissue Compatibility/Incompatibility: The Role of Acid Phosphatase in Lethal Cell Senescence in an Incompatible Heterograft

1980 ◽  
Vol 38 ◽  
pp. 530-531
Author(s):  
Randy Moore
Keyword(s):  
Acid Phosphatase ◽  
Thick Layer ◽  
Cell Necrosis ◽  
Enzyme Localization ◽  
Vegetative Plant ◽  
Cell Autolysis ◽  
Graft Interface ◽  
System 1 ◽  
Thin Fibril

Previous work has indicated that the graft incompatihility between Sedrmi telephoides and Solanum pennellil involves cell necrosis that results In a thick layer of collapsed cells at the graft Interface. This necrotic layer insulates the stock from the scion, which results in abscission of the Sedum scion after 4-6 weeks due to desiccation and starvation. Thus, cell autolysis (which is restricted to Sedum) characterizes the Incompatibility response in this system (1). In order to elucidate the events that lead to cell autolysis, and thus better understand the cellular site and mode of action of cellular incompatibility, the appearance and fate of the hydrolytlc enzyme acid phosphatase (AP) was followed in both the compatible Sedum autograft and the incompatible Sedum/Solanum heterograft. Acid phosphatase was localized by a modified Gomori-type reaction; positive (i.e., including NaF inhibitor) and negative (lacking substrate) controls showed no enzymatic precipitate. Following an initial association with the endoplasmic reticulum (ER) and dictyosomes at 6-10 hours after grafting, AP activity in the compatible Sedum autograft is associated primarily with the plasmalemma (Fig. 1). By 18-24 hours after grafting, the AP activity is restricted to the tono-plast and vacuole (Fig. 2). This strict compartmentation and absence of enzyme from the cytosol is maintained throughout the development of the compatible graft. While AP activity in the incompatible Sedum/Solanum heterograft is Initially similar to the compatible Sedum autograft (i.e., initially found on the ER and dictyosomes), there is a marked difference in enzyme localization in the two graft partners as the incompatibility response develops. As in the compatible autograft, Solanum cells at the graft interface show an Increase in AP activity that Is restricted to the vacuole and tonoplast, with little or no enzyme activity in the cytosol (Fig. 3). In comparable Sedum cells, however, there is a dramatic Increase In AP activity in the cytosol (Fig. h); this cytosollc AP activity is associated with thin fibril-like structures (Fig. 5) measuring approximately 60 A in diameter. This high cytoplasmic AP activity In Sedum cells results in cell autolysis, death, and eventual cell collapse to form the characteristic necrotic layer separating the two graft partners.

The Role of the Radioimmunoassay for Prostatic Acid Phosphatase in Prostatic Carcinoma

1980 ◽  
Vol 7 (3) ◽  
pp. 645-652 ◽  
Author(s):  
Andrew W. Bruce ◽  
Donald E. Mahan ◽  
William D. Belville
Keyword(s):  
Acid Phosphatase ◽  
Prostatic Carcinoma ◽  
Prostatic Acid Phosphatase

Role of acid phosphatase measurement in management of prostate cancer

Urology ◽  
1981 ◽  
Vol 17 (6) ◽  
pp. 550-553 ◽  
Author(s):  
Lester A. Klein ◽  
Philip Shapiro
Keyword(s):  
Prostate Cancer ◽  
Acid Phosphatase

Understanding the role of co-surfactants in microemulsions on the growth of copper oxalate using SAXS

2019 ◽  
Vol 21 (1) ◽  
pp. 336-348 ◽  
Author(s):  
Sunaina Sunaina ◽  
Vaishali Sethi ◽  
Surinder K. Mehta ◽  
Ashok K. Ganguli ◽  
Sonalika Vaidya
Keyword(s):  
Reverse Micelles ◽  
Copper Oxalate

SAXS study for evaluating the effect of variation of co-surfactants on the shape of reverse micelles and growth of copper oxalate nanostructures.

A study of kidney lysosomal acid phosphatase during compensatory renal hyperplasia in rats

1968 ◽  
Vol 46 (3) ◽  
pp. 499-502 ◽  
Author(s):  
B. M. Hegdekar
Keyword(s):  
Acid Phosphatase ◽  
Lysosomal Enzymes ◽  
Soluble Fraction ◽  
Female Rats ◽  
Enzyme Release ◽  
Lysosomal Fraction ◽  
Probable Role ◽  
Long Evans ◽  
Free Activity

Female rats of the Long-Evans hooded strain, 4–6 months old and weighing 275–300 grams, were subjected to unilateral nephrectomy and the acid phosphatase activity in the remaining kidney was studied at the end of 24, 48, 72 hours, and 8 days respectively. Most of the acid phosphatase was found in the particulate fraction in normal kidneys. The enzyme activity in the soluble fraction was found to have increased the second day after the operation, but decreased to the original level by the end of 72 hours. The free activity of the lysosomal fraction also increased by the end of second postoperative day. A change in the permeability of the lysosomal membrane before the enzyme release was observed. The probable role of lysosomal enzymes in the initiation of mitotic divisions during compensatory renal hyperplasia is discussed.

scholarly journals Enzyme variability and neonatal jaundice. The role of adenosine deaminase and acid phosphatase

1989 ◽  
Vol 17 (3) ◽  
pp. 195-201 ◽  
Author(s):  
Alessandro Lepore ◽  
N. Lucarini ◽  
M. A. Evangelista ◽  
G. Palombaro ◽  
A. Londrillo ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Adenosine Deaminase ◽  
Neonatal Jaundice
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