Differential expression of prolactin receptor (PRLR) in normal and tumorous adrenal tissues: separation of cellular endocrine compartments by laser capture microdissection (LCM)

1998 ◽  
Vol 24 (3-4) ◽  
pp. 857-862 ◽  
Author(s):  
A. Glasow ◽  
A. Haidan ◽  
J. Gillespie ◽  
P. A. Kelly ◽  
G. P. Chrousos ◽  
...  
Keyword(s):  
Differential Expression ◽  
Laser Capture Microdissection ◽  
Prolactin Receptor ◽  
Laser Capture

scholarly journals Quantitative Proteomic Study of Human Lung Squamous Carcinoma and Normal Bronchial Epithelial Acquired by Laser Capture Microdissection

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Xu Yan ◽  
Cao Lan-Qin ◽  
Jin Long-Yu ◽  
Chen Zhu-Chu ◽  
Zeng Gu-Qing ◽  
...  
Keyword(s):  
Differential Expression ◽  
Laser Capture Microdissection ◽  
Human Lung ◽  
Protein Profile ◽  
Squamous Carcinoma ◽  
Bronchial Epithelium ◽  
Target Cells ◽  
Proteomic Study ◽  
Lung Squamous Carcinoma ◽  
Laser Capture

Objective. To investigate the differential protein profile of human lung squamous carcinoma (HLSC) and normal bronchial epithelium (NBE) and provide preliminary results for further study to explore the carcinogenic mechanism of HLSC.Methods. Laser capture microdissection (LCM) was used to purify the target cells from 10 pairs of HLSC tissues and their matched NHBE, respectively. A stable-isotope labeled strategy using iTRAQ, followed by 2D-LC/Q-STAR mass spectrometry, was performed to separate and identify the differential expression proteins.Results. A total of 96 differential expression proteins in the LCM-purified HLSC and NBE were identified. Compared with NBE, 49 proteins were upregulated and 47 proteins were downregulated in HLSC. Furthermore, the expression levels of the differential proteins including HSPB1, CKB, SCCA1, S100A8, as well as S100A9 were confirmed by western blot and tissue microarray and were consistent with the results of quantitative proteomics.Conclusion. The different expression proteins in HLSC will provide scientific foundation for further study to explore the carcinogenic mechanism of HLSC.

Isolating Human Notochord Using Laser Capture Microdissection

2016 ◽  
Vol 77 (S 02) ◽  
Author(s):  
Youssef Yakkioui ◽  
Remco Santegoeds ◽  
Koo van Overbeeke ◽  
Andreas Herrler ◽  
Yasin Temel
Keyword(s):  
Laser Capture Microdissection ◽  
Laser Capture

scholarly journals Stromal MED12 exon 2 mutations in complex fibroadenomas of the breast

2020 ◽  
pp. jclinpath-2020-207062
Author(s):  
Edaise M da Silva ◽  
Francisco Beca ◽  
Ana Paula Martins Sebastiao ◽  
Melissa P Murray ◽  
Catarina Silveira ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Laser Capture Microdissection ◽  
Sanger Sequencing ◽  
Mediator Complex ◽  
Telomerase Reverse Transcriptase ◽  
Exon 2 ◽  
Stromal Component ◽  
Hotspot Mutations ◽  
Laser Capture

AimsHere we explore the presence of mediator complex subunit 12 (MED12) exon 2 and telomerase reverse transcriptase (TERT) promoter hotspot mutations in complex fibroadenomas (CFAs) of the breast.MethodsThe stromal components from 18 CFAs were subjected to Sanger sequencing of MED12 exon 2 and the TERT promoter hotspot loci. The epithelial and stromal components of two MED12 mutated CFAs were subjected to laser capture microdissection, and Sanger sequencing of MED12 exon 2, TERT promoter and PIK3CA exons 9 and 20, separately.ResultsMED12 exon 2 mutations were identified in the stroma of 17% of CFAs. The analyses of epithelial and stromal components, microdissected separately, revealed that MED12 mutations were restricted to the stroma. No TERT promoter or PIK3CA mutations in exons 9 and 20 were detected in analysed CFAs.ConclusionsLike conventional fibroadenomas, MED12 exon 2 mutations appear to be restricted to the stromal component of CFAs, supporting the notion that CFAs are stromal neoplasms.

scholarly journals Laser capture microdissection for transcriptomic profiles in human skin biopsies

2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Silvia Santoro ◽  
Ignazio Diego Lopez ◽  
Raffaella Lombardi ◽  
Andrea Zauli ◽  
Ana Maria Osiceanu ◽  
...  
Keyword(s):  
Human Skin ◽  
Laser Capture Microdissection ◽  
Skin Biopsies ◽  
Laser Capture

Thermal modeling of Laser Capture Microdissection

1998 ◽  
Vol 37 (31) ◽  
pp. 7378 ◽  
Author(s):  
Seth R. Goldstein ◽  
Philip G. McQueen ◽  
Robert F. Bonner
Keyword(s):  
Laser Capture Microdissection ◽  
Thermal Modeling ◽  
Laser Capture
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