Effects of Cannabinoids on Synaptic Membrane Enzymes. I.In vitroStudies on Synaptic Membranes Isolated from Rat Brain

1976 ◽  
Vol 3 (3) ◽  
pp. 485-505 ◽  
Author(s):  
Clinton A. Olmsted
Keyword(s):  
Rat Brain ◽  
Synaptic Membranes ◽  
Synaptic Membrane ◽  
Membrane Enzymes

Synaptosomal T3 binding sites in rat brain: their localization on synaptic membrane and regional distribution

1983 ◽  
Vol 104 (2) ◽  
pp. 134-138 ◽  
Author(s):  
Yasuo Mashio ◽  
Mitsuo Inada ◽  
Kiyoshi Tanaka ◽  
Hitoshi Ishii ◽  
Koichi Naito ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Rat Brain ◽  
Binding Sites ◽  
Binding Capacity ◽  
Regional Distribution ◽  
Brain Regions ◽  
Synaptic Membranes ◽  
Synaptic Membrane ◽  
Scatchard Analysis ◽  
Affinity Binding

Abstract. Previous studies have shown the existence of specific high affinity T3 binding sites in rat cerebrocortical synaptosomes. In this study, to define the localization of the binding sites, T3 binding to disrupted synaptic membrane fractions was compared with the binding to intact synaptosomes obtained from the rat cerebral cortex. Scatchard analysis revealed two orders of T3 binding sites with almost identical apparent dissociation constant (Kd) in synaptosomes and synaptic membrane fractions. However, the maximal binding capacity (MBC) of the higher affinity binding sites for synaptic membrane fractions was significantly greater than that for intact synaptosomes (4.2 ± 0.2 vs 3.0 ± 0.3 pg T3/mg protein). Regional distribution of the synaptosomal T3 binding sites in various areas of the rat brain was also studied. The Kd values were not significantly different in discrete brain regions. On the other hand, the MBCs of the higher affinity binding sites were greater in the cerebral cortex and hypothalamus (72.8 ± 1.4 and 64.8 ± 9.5 pg T3/g tissue) than in the cerebellum (22.1 ± 1.6 pg T3/g tissue). Similar difference was also observed in the lower affinity sites. These results indicate that the specific T3 binding sites in brain synaptosomes are localized mainly on synaptic membranes and that the MBC is different in discrete brain regions.

Developmental alteration of rat brain synaptic membranes. Reaction of glycoproteins with plant lectins

1979 ◽  
Vol 165 (2) ◽  
pp. 283-293 ◽  
Author(s):  
Nihal S. De Silva ◽  
James W. Gurd ◽  
Charles Schwartz
Keyword(s):  
Rat Brain ◽  
Synaptic Membranes ◽  
Plant Lectins

Specificity of prostaglandin D2 binding to synaptic membrane fraction of rat brain

1986 ◽  
Vol 362 (1) ◽  
pp. 114-121 ◽  
Author(s):  
Hidekado Tokumoto ◽  
Yasuyoshi Watanabe ◽  
Akira Yamashita ◽  
Yoshinobu Arai ◽  
Osamu Hayaishi
Keyword(s):  
Rat Brain ◽  
Membrane Fraction ◽  
Synaptic Membrane ◽  
Prostaglandin D2

scholarly journals Dopamineinduced up-regulation of .ALPHA.2-adrenoceptors in crude synaptic membranes of rat brain.

1985 ◽  
Vol 85 (3) ◽  
pp. 173-183 ◽  
Author(s):  
Yasuko KOHNO ◽  
Yasuyuki NOMURA ◽  
Tomio SEGAWA
Keyword(s):  
Rat Brain ◽  
Synaptic Membranes
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