scholarly journals DAIRY PRODUCTIVITY OF FIRST COWS WITH DIFFERENT GENOTYPES BY GROWTH GENE

2021 ◽  
Vol 61 ◽  
pp. 119-125
Author(s):  
V. V. Dzitsiuk ◽  
I. D. Mitioglo ◽  
N. B. Мochnachova ◽  
M. L. Dobrjancka

The aim of the study was to study the allelic polymorphism of the growth hormone gene in a group of cows of the Montbeliard breed and to search for associations of its different genotypes with the parameters of the dynamics of milk yield during lactation. The research was conducted in the Department of Animal Genetics and Biotechnology of the Institute of Animal Breeding and Genetics of NAAS on a group of 30 cows of the Montbeliard cattle breed from PSP "Zhadkivske" of Chernihiv region. Genomic DNA was isolated from blood leukocytes by standard methods using the set "DNA-sorb-B" ("Ampli-Sense", Russia). Dairy productivity of first-born cows was determined monthly by control milkings. Types of lactation curves were studied according to the method of A. S. Emelyanov (1953). Polymorphism of the growth hormone (GH) gene in our first-born cows is represented by two alleles L I V, the frequency of which differs, and the frequencies of genotypes differ accordingly. In the studied group of animals, all possible genotypes of the growth hormone gene were identified: 25 animals (83%) have the GHLL genotype, 4 (13%) have the GHLV genotype, and only one cow has the GHVV genotype. The frequency of the L allele is 0.9, and the V allele is 0.1. The mean expectation for 305 days of the first lactation of a group of cows with the GHLL genotype was 352 kg higher than the expectations in the group of heterozygous cows with the GHLV genotype. In terms of milk fat and protein yield, cows with GHLL genotype were significantly dominated by cows with GHLV genotypes (74 kg fat and 36 kg protein, respectively, p < 0.001). Analysis of the dynamics of monthly milk yield of the studied cows for 305 days of the first lactation showed that the productivity of cows with GHLL genotype exceeds the average monthly milk yield of other groups of animals at the beginning (from 1 to 3 months), at the peak of lactation (4th month) and at the end of lactation (from 8 to 10 months). In general, during the 305 days of the first lactation, first-borns with the GHLL genotype had better milk yields compared to their peers with other growth hormone gene genotypes. Analysis of variance showed that the obtained share of variability in milking in animals with different genotypes of the GH gene is due to random factors. Analysis of lactation curves of primiparous women showed that milk yield of cows with all variants of genotypes in the GH gene gradually increased and reached its peak at 3–4 months of lactation, and then gradually decreased. This lactation curve, according to the classification of A. S. Emelyanov is characteristic of animals with high and stable lactation, and cows are able to digest food well and produce high hopes. \ Thus, a statistically significant difference in quantitative and qualitative characteristics of milk productivity of cows of Montbeliard breed with different variants of genotypes by GH gene on the example of a group of cows from "Zhadkivske" Chernihiv region suggests that the use of genetic markers, in particular the growth hormone GH selection of selection and further formation of the herd in the direction of increasing milk productivity.

Author(s):  
Л.Р. ЗАГИДУЛЛИН ◽  
Р.Р. ШАЙДУЛЛИН ◽  
Т.М. АХМЕТОВ ◽  
С.В. ТЮЛЬКИН ◽  
А.Б. МОСКВИЧЕВА

Изучена взаимосвязь аллельных вариантов генов пролактина и соматотропина с показателями молочной продуктивности коров черно-пестрой породы по 1 и 3 лактации. У первотелок, как и у полновозрастных животных,  наиболее высокий удой (4642 и 6240 кг), количество молочного жира (174,1 и 215,0 кг) и молочного белка (149,0 и 182,5 кг) достигнуты в группе с генотипом PRL АА. По массовой доле жира и белка в молоке коровы с генотипом PRL ВВ достоверно превосходили животных с генотипом PRL АА на 0,12% (Р<0,05) и 0,05% (Р<0,05) соответственно. По гену соматотропина наибольший уровень молочной продуктивности выявлен у коров с генотипом GH LL при достоверном преимуществе над GH VV у первотелок по удою на 439 кг (Р<0,01), выходу молочного жира — на 13,6 кг (Р<0,001), выходу молочного белка — на 15,3 кг (Р<0,01). У полновозрастных коров превышение над остальными группами было только по удою — на 219—548 кг (Р<0,05). Наибольшая жирномолочность характерна животным с генотипом GH VV по 1 лактации — 3,83% и по 3 лактации — 3,82%, а лучшая белковомолочность — коровам, имеющим аллель V гена GH:  3,21 и 3,23% соответственно. Полученные данные свидетельствуют о более высоком уровне молочной продуктивности у коров с генотипом PRL АА и GH LL, но лучшая жирномолочность и белковомолочность отмечены у особей, имеющих в своем генотипе аллели В гена PRL и V гена GH. The interrelation of allelic variants of prolactin and somatotropin genes with indicators of milk productivity of black-and-white cows for the 1st and 3rd lactation was studied. The highest milk yield (4642 and 6240 kg) amount of milk fat (174.1 and 215.0 kg) and milk protein (149.0 and 182.5 kg) were in the group with the PRL AA genotype in first-calf heifers as well as in full-age animals. In terms of the mass fraction of fat and protein of milk, the cows with the PRL BB genotype significantly exceeded animals with the PRL AA genotype by 0.12% (P<0.05) and 0.05% (P<0.05), respectively. According to the somatotropin gene, the highest level of milk productivity was revealed in cows with the GH LL genotype with a significant advantage over GH VV in first-calf heifers in milk yield by 439 kg (P<0.01), milk fat yield by 13.6 kg (P<0.001), milk protein yield by 15.3 kg (P<0.01). The excess over the other groups in full-age cows was only in terms of milk yield that was by 219-548 kg (P<0.05). The highest protein content of milk is a characteristic of animals with the GH VV genotype, 3.83% is for the 1st lactation and 3.82% is for the 3rd lactation, and the best protein content of milk in cows with V allele of the GH gene is 3.21 and 3.23%, respectively. The obtained data  indicated a higher level of milk productivity in cows with the PRL AA and GH LL genotypes but the best fat and milk content was noted in cows with alleles B of the PRL gene and V of the GH gene in their genotype.


2020 ◽  
Vol 59 ◽  
pp. 51-59
Author(s):  
A. P. Krugliak ◽  
T. O. Krugliak

The results of analytical studies of breeding value variability on the basis of milk productivity, selected for reproduction of bulls of different genealogical formations of Holstein breed, are presented. A statistically significant difference between the breeding value of the milk yield of bulls and their parents of different genealogical groups was established. The variability of bulls breeding traits and the milk productivity of their daughters in the middle of genealogical formations was dominated by similar indicators between genealogical formations. In the middle of related genealogical formations, animals of new generations significantly outnumbered animals of earlier generations in level of breeding value. The high correlation between breeding value of milk yield, milk fat and protein of parents and their sons was identified. The highest correlation coefficient (r = +0.643 ± 0.030) was established between the absolute milk productivity of daughters for 305 days of first lactation and the level of breeding value of milk yield of their parents, which decreased sharply when compared with their ancestors of older generations. It was also established, that the breeding value of the animals of the new genealogical formations (sons, fathers, mothers) of Tradition 1682485 and Valiant 1650414 was always higher (on 161.2; 121.4; 209.4 kg worth), compared to the older related groups of Elevation and Chief, on which basis they have been formed. This indicates about decreasing of appearance frequency of leader bulls in a single related group during breeding. The sons of these bulls inherit the signs of milk productivity, their breeding value exceeded that of their parents: by milk fat – by 2.4–20.0 kg, total protein – by 0.7–11.8 kg. The absolute milk production of their daughters in the 305 days of the first lactation was 11201–13173 kg of milk, 411–475 kg of milk fat and 337–383 kg of total protein, which exceeded the productivity of their contemporarys by 113–1025 kg of milk, 8.6–55.6 kg milk fat and 9.3–30.3 kg total protein. A similar predictable of variability of the breeding value of animals of different genealogical formations of the Holstein breed for milk fat and protein in milk was established. The level of breeding value of the fathers has the greatest influence on the level of milk production of their daughters of the Holstein breed (the influence force is 35.1%). In second place was the force of influence breeding value of the father-fathers on the milk yield of their granddaughters, which was 11.7%. The third place was occupied by the influence of the breeding value of mothers on the level of milk productivity of their daughters (η2х = 10,6%). These forces of influence of these ancestors on the level of milk productivity of the firstborn cows are statistically significant (P < 0.05–0.001). The last place of force of influence (η2х) on the level of milk productivity of the firstcalve heifers take the male ancestors, who are in their third line of pedigree (FFF), it was 2.7% and was not statistically significant.


Author(s):  
Faisal M. Aqlan ◽  
Yaaser Q. Almulaiky ◽  
Mohammed Alkhaled ◽  
Aisha A.M. Alayafi ◽  
Haytham A. Ali ◽  
...  

This study was aimed to evaluate the relationship between growth hormone (GH) gene polymorphism and milk production in Najdi sheep. Two hundred Najdi ewes were used to determine the daily milk yield (DMY). The complete CDS of the Najdi sheep GH gene is1950 in length, GenBank acc. no. KU310891.1. Najdi sheep GH protein consists of 217 amino acids GenBank ALT22407.1 with conversion amino acid arginine number 121 to amino acid lysine (R121K). A novel five SNPs were detected in Najdi sheep GH gene (G871A, A1259C, G1383A, A1486G and A1509G). A significant association between the detected SNPs genotypes and daily milk yield was found in this study. The regression analysis indicated the positive effect of genotype and SNPs on DMY. Individuals carrying homozygote mutant alleles had the highest DMY. Consequently, these SNP may be useful indicators in the selection of ewes for milk production.


2018 ◽  
Vol 51 ◽  
pp. 170-177 ◽  
Author(s):  
L. M. Khmelnychyi ◽  
V. V. Vechorka

At the base of a breeding farm in Cherkassy region, with the modern highly mechanized technologies of milk production, five groups of cows – the crossbred genotypes of Ukrainian Red-and-White Dairy breed with a gradation of 12,5% blood share of Holstein (I – 37,5-50,0; II – 50,1-62,5; III – 62,6-75,0; IV – 75,1-87,5; V – 87,6-100,0) were studied. The aim of this study was to explore the impact of Holstein heredity on indicators of life expectancy, economic use and lifetime productivity of cows. The results of retrospective analysis proved significant effect of Holstein conditional heredity on indicators of life expectancy, economic use and lifetime productivity of the Ukrainian Red-and-White Dairy cows of the experimental farm. The best among all five groups by indicators of life expectancy, economic use, number of lactations, lifetime milk yield and yield of milk fat, and milk yield for one day were the crossbred genotypes of the second group with conditional heredity of Holsteins 50,1-62,5 %. The advantage of the second group of animals with Holstein heredity from 50,1 to 62,5 % differed significantly in life expectancy and economic use exceeding other groups of animals by these traits by 451-1449 and 465-1472 days respectively with a significant difference at P<0,001, except their comparison with the first group. The animals of this group were used the longest in the herd, during five lactations and characterized by the highest coefficient of economic use and it is higher by 1,2-3,1 lactation and by 6,0-20,7 coefficient of economic use respectively, with degree of reliability at P<0,01-0,001 compared with all the groups of crossbred genotypes. For lifetime milk productivity advantage was also in favor of crossbred animals with Holstein heredity from 50,1 to 62,5%. If milk yields of crossbred genotypes of the first and third groups were less than the yield of animals of the second group only by 5571 and 5848 kg (P<0,05 and 0,01), then for high-blood genotypes of the fourth and fifth groups they were less by 11813 and 14021 kg at P<0,001 or in 1,7 and 2,0 times. In general, cows with the highest share of Holstein blood (87,6-100,0%), and this is genotype which will have a dominant distribution in the array of Ukrainian Red-and-White Dairy breed at least for one or maximum for two generations, with 305-day milk yield of first-calf heifers of 5677 kg and for one day of economic use 14,0 kg of milk exceeded other groups of crossbred genotypes with degree of reliability by 455-806 (P<0,001) and 0,8-2,5 (P<0,01-0,001) kg of milk respectively; it unconditionally testifies to positive influence of Holstein heredity on these traits. The best crossbred genotypes by indicators of life expectancy, economic use, number of lactations and lifetime productivity were with 50,1-62,5% share of Holstein blood in the herd of Ukrainian Red-and-White Dairy cattle. Productivity of crossbred cows per lactation increased with growing share of Holstein blood, but indicators of longevity and lifetime productivity decreased significantly, especially at high-blood animals with heredity of improving breed higher than 75,1%.


Author(s):  
V. Ladyka ◽  
Y. Sklyarenko ◽  
Y. Pavlenko

In the herd of cattle of the Ukrainian Brown Dairy breed studies were conducted, the purpose of which was to study the influence of the genotype of cows by kappa-casein on the indicators of their milk productivity. Genotyping of 29 heads of cattle. Determination of the polymorphism of the kappa-casein gene was performed in the genetic laboratory of the Institute of Physiology n.a. Bogomolets of NAS using molecular biological analysis of allele recognition by polymerase chain reaction (PCR) in real time. Allele A had a slightly higher frequency - 0.517 compared to allele B - 0.483 at the locus of the kappa-casein gene, although it was not significant. As a result, the proportions of AA and BB genotypes were high (38 and 34%, respectively). The performed genetic-statistical analysis revealed an excess in the kappa-casein locus of homozygous variants AA and BB and a lack of heterozygous AB. The degree of homozygosity, which is 50.1%, indicates a sufficient level of consolidation in the studied population of cattle. The number of effective active alleles in the kappa-casein locus of cows of the Ukrainian Brown Dairy breed is 1.99, with the maximum value of the level of polymorphism possible at a two-allele locus of 2. A negative heterozygosity test (HT) indicates a lower proportion of actual heterozygotes relative to the proportion of theoretical heterozygotes. The reason for the existing genetic structure of the herd of Ukrainian Brown Dairy breeds by the kappa-casein locus was the use of Swiss cattle breeders, most of which were not evaluated by the genotype of the kappa-casein gene. Studies of the level of milk productivity of cows of different genotypes by kappa-casein in the first lactation did not reveal a significant difference. At the same time, heterozygous (AB) animals were inferior to homozygous (AA and BB) in terms of milk yield, and animals with AA and AB genotypes were preferred in terms of quality. For the best lactation, a significant difference was found in the amount of milk yield and the amount of milk fat. Homozygous animals (AA and BB) significantly (p <0.05) outperformed heterozygous (AB) in milk yield, by 1091 and 922 kg, respectively. Animals with heterozygous AB genotype in terms of the average amount of milk fat were significantly inferior to homozygous AA and BB animals (p <0.05). The small number of experimental animals was one of the reasons for the discrepancy between the results of research and the data of other scientists. Key words: breed, milk yield, fat content, protein content, kappa casein, genotype, allele.


Author(s):  
A. Zheltikov ◽  
N. Kostomakhin ◽  
D. Adushinov ◽  
O. Zaiko ◽  
V. Dementev ◽  
...  

The characteristic of milk productivity of cows of Holstein and Simmental breeds in LLC “Sibirskaya Niva” in the Maslyaninsky district in the Novosibirsk region has been given. It has been revealed that under the natural and climatic environments of the Western Siberia, when creating favorable conditions for feeding and housing for cows of Holstein and Simmental breeds have sufficiently high milk productivity. It has been found that Holstein first-calf heifers surpassed their Simmental herdmates by 959 kg in milk yield for the first 305 days of lactation under the same conditions of housing and feeding, by 3,1 kg in daily milk yield, by 38,2 kg in milk fat yield, and by 28,3 kg in milk protein yield (P < 0,001). There were no statistically significant differences between animals of Holstein and Simmental breeds in terms of fat and protein content in milk, which amounted to 3,94 and 2,96 % in Holstein breed and 3,97 and 2,98 % in Simmental breed, respectively. Both breeds have shown high milk productivity during the first 3 lactations. Thus, the milk yield of Holstein cows for the first 3 lactation was 6475–9290 kg, Simmental cows it was 5516–7680 kg, fat content was 3,93–3,99 and 3,96–3,97 %, respectively. The protein content in the milk of cows of both breeds did not exceed 3,0 %. High variability has been found in milk yield, yield of milk fat and protein, the coefficient of milk content and duration of lactation, the lowest in the content of protein and fat in milk and live weight. The correlation coefficients between the fat and protein content in milk were generally positive, but did not exceed 0,28. Therefore, breeding in the herd of cows in LLC “Sibirskaya Niva” have to be conducted not only by fat content, but also by protein content in milk.


Combination of genotypes of main types’ genetic markers causes milk productivity in cattle. The article studies correlation between kappa casein genotypes (C3N3), beta-lactoglobulin (LGB), their complexes and milk production indices in cows bred under equal conditions at a breeding farm. The calculation of parental milk yield and fat indices between the three breeds showed a significant difference (P>0,999) of PCI of Ayrshire and Holstein cattle breed milk yield - 3395 kg, PCI of fat - 0,22%; between Holstein and Yaroslavl cattle breeds - 3200 kg, PCI offat - 0,16%. A significant difference of realization indices of protein between all three breeds was not stated. The highest realization ofparental milk yield index was stated - between Ayrshire and Yaroslavl cattle breeds- 113%, Holstein cattle breed - 88%. As for the fat and protein indices, all three cattle breeds showed a high realization of PCI from 98% to 109%. However, Holstein cattle breed showed the highest percentage: protein - 101%, fat - 109%. A significant difference of PCI realization of milk productivity in CSN3 and LGB genotypes was not stated. It was also stated that there is a tendency of high level parental indices realization when there is a higher number of B-allele variants in genotypes of cows.


1992 ◽  
Vol 12 (6) ◽  
pp. 2624-2632
Author(s):  
D Murphy ◽  
K Pardy ◽  
V Seah ◽  
D Carter

In thyroid hormone-depleted rats, the rate of transcription of the growth hormone (GH) gene in the anterior pituitary gland is lower than the rate in euthyroid controls, and there is a corresponding reduction in the abundance of the GH mRNA. Concomitantly, the poly(A) tail of the GH mRNA increases in length. Examination of nuclear RNA from anterior pituitary glands of control and thyroid hormone-depleted rats revealed no difference in the length of pre-mRNAs containing the first and last introns of the GH gene. However, mature nuclear GH RNA is differentially polyadenylated in euthyroid and hypothyroid animals. We suggest that the extent of polyadenylation of the GH transcript is regulated in the cell nucleus concomitant with or subsequent to the splicing of the pre-mRNA. Experiments with anterior pituitary gland explant cultures demonstrated that the GH mRNA from thyroid hormone-depleted rats is more stable than its euthyroid counterpart and that the poly(A) tail may contribute to the differential stability of free GH ribonucleoproteins.


2021 ◽  
Vol 250 ◽  
pp. 172-177
Author(s):  
E. I. Anisimova E. I. ◽  
◽  
P. S. Katmakov ◽  
A. V. Bushov ◽  
◽  
...  

The paper presents results of assessing black-and-white bulls by the quality of the offspring with application of different methods. The evaluation of bulls was carried out with application of the “daughter-mother” method in the conditions of Volzhsky Research Institute of Hydraulic Engineering and Land Reclamation. The valuation showed that the bulls Aist 7 and Muscat 8 increased milk yield of daughters, in comparison with mothers, by 951 and 486 kg, or by 26.7 and 12.9%. The rest of the servicing bulls reduced milk yield of the offspring by 46 - 1019 kg (1.1 - 24.7%). When assessing the bulls using the “daughterpeer” method, the highest milk productivity was shown by the descendants of Sazan 25 and Aist 7 of Vis Back Ideal line, which surpassed their peers in milk yield by 273 and 419 kg, or by 3.8 and 5.7%, respectively. The producers Snop 57 (his 17 daughters had average milk yield of 4224 kg for the first lactation with milk fat of 3.80%) and Muscat 8 (with an average milk yield of daughters of 4254 kg , milk fat - 3.83%) are also of significant value for the breeding work of this farm. According to the assessment results, the producer Aist 7 was assigned to the breeding category of A1 of milk yield improvers. Bulls Sazan 25 and Muscat 8 are also rated as daughters’ milk yield improvers and are categorized as A2 and A3. All bulls evaluated by comparing daughters to the breed standards are improvers of daughters’ milk yield (+ 454-1860 kg).


2017 ◽  
Vol 53 ◽  
pp. 222-227
Author(s):  
T. M. Komendant

Objective: to study the dependence of productive longevity of cows of black and motley breed on genetically determined factors - linearity and bloodiness in Holstein breed. Methods: zootechnical, biological, statistical. The materials of the studies were pedigree cards of cows  and bulls, bull catalogs, zootechnical reports on pedigree work with the herd, the cattle base of the Grodno region, and also registers of rearing and rearing of the litter. To study the effect on the longevity of cows and their lifelong productivity of the "linear accessory" factor, all animals that were eliminated in 2006-2014 were divided into groups according to the lines: Adema 25437, Annas Adema 30587, Vis Eidial 933122, Montvik Chiftein 95679, Nico 31652 , Pabst Governera 882933, Reflection Sovering 198998, S. Sensation 1267271, Hiltes Adema 37910. Depending on the bloodiness of the Holstein breed, the animals were divided into four groups: I - up to 25% of Holstein genes, II- 25,0 – 49,9% of the genes of the Holstein genus, III - Holstein blood 50% and more, IV - purebred black-motley cows. Indicators of milk productivity (life-time milk yield (kg) and lifetime yield of milk fat (kg), milk yield for 1 day of lactation (kg), total duration of the lactation period (days) were studied in the experimental animals, and the duration of economic use (lactations). Main results. The results obtained during the statistical processing of data indicate that the cows belonging to the Pabst Governera line 882933 differed in the longest period of economic use among the abandoned animals. Duration of use in animals of this line was 8.43 lactations, which is more than for cows of other lines at 1,72-6,67 lactation. It should be noted that cows belonging to the Adem 25437 lines (6,71 lactations) and S. Sensation 1267271 (5,49 lactations) were also characterized by a rather long period of economic use. Due to the long period of use of the cow, the Pabst Governera line 882933 had the highest indicator of lifelong milk yield – 51535,58 kg of milk and the yield of milk fat – 1914,03. But as for milk for one day of lactation, here the leadership belonged to individuals belonging to the line Vis Eidial 933122 and amounted to 21,19 kg of milk per day. Also, a fairly high indicator of milk yield for one day of lactation was observed in animals belonging to the line Montvik Chiftain 95679 – 21,02 kg of milk. As a result of the research it was found that the largest term of economic use was characterized by low-breed hybrids with Holstein breed (up to 25%) – 3,63 lactations. This group of animals exceeded the individuals of the remaining groups by 0,21-0,76 lactations. It is worth noting that purebred black-motley individuals also differed by a long period of productive use – 3,42 lactations. Conclusions. Analysis of the impact on productive longevity and indicators of lifetime productivity of the "linearity" factor among abandoned animals in the "Progress-Vertelishki" made it possible to establish the existence of significant differences in the duration of economic use of cows of different linear affiliation. Cows of the Pabst Governera line 882933 – 8,43 lactations, 51535,58 kg of milk and 1914,03 kg of milk fat were distinguished by the greatest period of productive longevity, and also by the highest milk yield and milk fat content. It was established that the crossing of black and white cattle with Holstein breed positively influenced the productive longevity of animals, but on condition that the blood on the improving rock did not exceed 25%. Low-breed hybrids with Holstein breed exceeded the animals of other groups by the duration of their economic use by 0,21-0,76 lactations. Thus, we recommend breeding cows of black and motley breeds in the "Progress-Vertelishki", taking into account the impact on productive longevity and milk productivity of such factors as linearity and bloodiness in Holstein breed.


Sign in / Sign up

Export Citation Format

Share Document