scholarly journals OPTIMIZATION OF THE METHOD OF DNA ISOLATION FROM FOSSILS

2020 ◽  
Vol 60 ◽  
pp. 110-115
Author(s):  
N. B. Mokhnachova ◽  
L. F. Starodub ◽  
M. L. Dobryanska
Keyword(s):  
Research Methods ◽  
Dna Isolation ◽  
Molecular Genetic ◽  
Domestic Animals ◽  
Issr Markers ◽  
Domestic Horse ◽  
Dna Fragments ◽  
Wild Horse ◽  
History Of ◽  
The Village

The history of the origin and domestication of farm animals has always interested mankind. However, these issues are covered in the literature in great detail only from the time when herds of domestic animals have already formed. Most often, the genesis of individual species, the original forms that formed the basis of domestication, remain unclear. [2] An example is the history of domestication of the horse, as the horse played a central role among other domestic animals in the development of human society. In the study of mammal fauna of the Pleistocene-Holocene of Europe there is a problem of studying the origin of the domestic horse Eguus cabalus L., ie, the establishment of wild ancestors of domesticated breeds, place, time and process of their domestication. Analysis of literature data on paleontological and archaeological finds in Ukraine showed that most researchers believe that the first domesticated horses began to recognize horses, the remains of which were found during archaeological excavations of the settlement of the third millennium BC. BC in Botai (Northern Kazakhstan), but from which taxon the opinions of scientists differ. Some believe that it could be Tarpan, however, there is an opinion that a large horse could not come from a small tarpan and Przewalski's horse. Therefore, preference was given to the hypothesis of the origin of the domestic horse from the ancient Pleistocene. At present, the problem of the origin of the domestic horse does not go beyond hypotheses and assumptions, and this is primarily due to the slight difference between the bones of the domestic and wild horse. The plasticity of the skeleton of the genus Eguus is very weak and this explains the problems faced by paleontologists in trying to develop the evolutionary history of horses. Thus, to understand the processes of domestication of this animal, in addition to archaeological and paleontological research methods, it is necessary to use tools from other fields of science, such as molecular genetic analysis of DNA samples. One of the variants of test systems for studying genetic polymorphism is the use of ISSR markers, which allow to analyze DNA fragments and make certain phylogenetic connections in the studied groups. In the laboratory of genetics of the Institute of Breeding and Genetics of Animals named after M.V.Zubets NAAS began research in the field of paleogenetics, namely – the study of the molecular genetic component in the fossils of ancient members of the genus Eguus using ISSR-markers. Inverted repeats are of particular interest because they are unevenly distributed throughout the genome and do not require prior knowledge of the nucleotide sequence of the test DNA. A significant point in the selection of research methods for us was that intermicrosatellite polymorphism is used to study interspecific and intraspecific genetic variability. It is believed that DNA fragments obtained by ISSR analysis can be species- and breed-specific, and this method is widely used by researchers in the study of breed groups. The purpose of our work is to develop a new method of DNA isolation from fossil remains (bones) of ancient horses and the production of ISSR-PCR with isolated DNA samples in the laboratory of genetics IRGT. M.V.Zubets NAAS according to the available reagents and existing protocols. The research was carried out on samples of fossil bones of horses of the Pleistocene period (about 10 thousand years BC). One bone was found in the village. Beeches of Zhytomyr region in a career. Excavations were carried out in 1960, the metacarpal bone (os. Tarsicentral). Another bone was found in Novgorod-Siversky, Chernihiv region. in a career. Excavations were conducted by Boriskovsky PI in 1935. A tooth found in the village of Tarpan was used to study a wild tarpan horse (4.5 thousand years BC). Skibnytsia, Trostyanets district, Vinnytsia region. Excavations were conducted in 1959 by VM Danylenko. The paleontological material for the study was provided by the Kyiv National Museum of Natural History of the National Academy of Sciences of Ukraine, Department of Paleontology. As a result of this work for the first time in the Department of Genetics and Biotechnology IRGT. Research on paleogenetics has been started by M.V.Zubets. We optimized the method of extracting genetic material from fossils and obtained DNA from the bones of a horse of the Pleistocene period (about 10 thousand years BC) and the tooth of a wild horse tarpan (4.5 thousand years BC). Also, the optimal conditions for PCR were selected to work with DNA obtained from fossil remains, to study polymorphism with ISSR markers, and electrophoregrams of amplification products were obtained.

scholarly journals Dinamika Peradaban Kampung di Bangka

2019 ◽  
Vol 10 (2) ◽  
pp. 190-213
Author(s):  
Rusydi Sulaiman
Keyword(s):  
Qualitative Research ◽  
Research Methods ◽  
Subject Matter ◽  
Archaeological Data ◽  
Colonial Policy ◽  
History Of ◽  
The Subject ◽  
The Right ◽  
The Village ◽  
Vast Territory

Indonesia is known for its vast territory consisting of thousands of islands. One of them is Pulau Bangka—an  island which also includes many villages in the right and left row of roads with other completeness as the reality of the village in Bangka. Sociologically, the island is harmed because the form of the village in Bangka is more due to colonial policy in the middle of  nineteen century. It was , different from previous village land inherited by the ancestors (Atok-Nek) in Bangka. This article focuses on early village discussions in Bangka named kampung  with qualitative research methods sourced from data  related to the subject matter.  This research produces several sub-discussions, namely: village philosophy, archaeological data in Bangka, the  history of kampung in Bangka,  kampung and strengthening civilization. Kampung does not appear immediately in the history of Bangka, but there were in long process. Early Bangka people inherited some ranges; memarung, panggung, bubung kampung and nganggung. Then adopted by Malay Islam (urang lah. In philosophical Islam, there was a process of strengthening local wisdom values  as a form of kampung civilization in Bangka  island.

scholarly journals MOLECULAR GENETIC PLANT ANALYSIS, ARTEMISIA L. GENUS, WITH ISSR-MARKERS

REPORTS ◽  
2020 ◽  
Vol 6 (334) ◽  
pp. 35-41
Author(s):  
Zh.Sh. Rakhymberdieva ◽  
◽  
A.N. Kaliyeva ◽  
G.D. Medeuova ◽  
◽  
...  
Keyword(s):  
Genetic Relatedness ◽  
Molecular Genetic ◽  
Issr Markers ◽  
Molecular Genetic Analysis ◽  
Universal Primers ◽  
Plant Analysis ◽  
The Road ◽  
North East ◽  
Repeat Units ◽  
The Village

. Molecular genetic analysis of three plants of thistle family has been carried out in the scope of this paper (Artemisia karatavica Krasch. & Abolin ex Poljakov, Artemisia cina Berg ex Poljakov, Artemisia porrecta Krasch. ex Poljakov). The plants have been collected in Turkestan region, Shardarinsky district, 15 km north-east of the village Komsomol, and along the road in Turkestan region, Baydibeksky district, 4.5 km south-east of the village Shakpak, and in the steppes in Turkestan region, Aryssky district, 1 km north-east of the village Darmino. In this paper, we used modern methods of molecular biology in order to determine genetic relatedness. ISSR analysis using universal primers has been conducted. ISSR-markers are the most common markers, and they are used for phylogenic analysis. This method is based on amplification of sequences limited by two microsatellite repeats using the primer that is complementary to the sequence of this microsatellite (4-12 repeat units). ISSR (region of the genome between two adjacent, oppositely oriented microsatellites) the sequence of microsatellite medullar part with some (1-3) nucleotides adjacent to the repeat tandem are used as primers. Tens of fragments of locus variety received in PCR are separated by electrophoresis and assessed for the presence or absence (due to marker dominance) of the fragments of a particular size. The main advantage of this type of markers - lack of necessity for knowledge of the sequences during primer designing.

scholarly journals SELECTION AND ASSESSMENT OF ISSR-MARKERS FOR ANALYSIS OF SEPARATE CATTLE POPULATIONS

2021 ◽  
Vol 61 ◽  
pp. 137-145
Author(s):  
О. M. Мaherovska
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Dairy Cattle ◽  
Dna Sequences ◽  
Biological Material ◽  
Dna Polymorphism ◽  
Molecular Genetic ◽  
Issr Markers ◽  
Dna Fragments ◽  
Pcr Products

The article covers molecular genetic analysis of intermicrosatellite DNA sequences of dairy cattle productivity. Molecular markers based on DNA polymorphism were used for this monitoring. Such markers make it possible to assess quickly the genetic polymorphism of taprin in the herd. Іnsofar as a large number of intermicrosatellite repeats is in the genome of cattle, that increases the probability of detecting polymorphic loci. The ISSR markers selected for the study are based on multiclocus synthesis in polymerase chain reaction (PCR) and allow an objective study of the breed and interbreed diversity. And it makes possible to assess quickly and accurately genetic diversity for the presence of genes associated with economically useful traits. The purpose of this work is the selection and evaluation of ISSR-markers for the analysis and study of genetic diversity of Ukrainian and imported breeds of dairy cattle. Samples of biological material from representatives of three herds of cattle (Ukrainian Red-and-White spotted dairy, Montbeliard breed and their crossbreeds) were selected for the study by the method of groups-analogues. For the analysis of this material the generally accepted zootechnical methods of studying of a selection material and methods of an estimation of animals on molecular – genetic markers are included. According to standard methods, DNA was isolated from peripheral blood lymphocytes using a set of reagents "DNA Sorb B". Amplification of total DNA with ISSR primers was performed on a thermal cycler "Tertsyk" ("DNA technology" of the Russian Federation). Electrophoretic separation of DNA fragments was performed in an agarose gel according to conventional methods. The size of the obtained PCR products was detected using a molecular weight marker SM1343. As a result of the study of the biological material of these animals, the obtained ISSR-PCR products were quite heterogeneous. The vast majority of polylocus spectra had clear discrete bands, but there were amplicons without clear discrete bands. Analyzing the results of the study of the genetic structure of animals of the Ukrainian Red-and-White dairy breed, using primers ISSR-1, ISSR-2, ISSR-3 and ISSR-4, the range of obtained PCR products ranges from 250 bp. up to 3000 bp. The range of amplification products in Montbeliard animals has a smaller range and ranges from 250 bp, respectively. up to 1500 bp.The obtained amplicons for the use of primers ISSR-1 and ISSR-2, ISSR-3 and ISSR-4 in the turf of Ukrainian Red-and-White dairy and Montbeliard breeds have sizes from 350 bp to 2000 bp. Having analyzed the information you can determine the distribution of the number and length of DNA fragments using 4 ISSR-markers. The total number of amplified DNA fragments varies depending on the primer from 21 to 106, and their size ranges from 250 BP up to 3000 bp. Based on the analysis of DNA plymorphism, it is possible to assess the heterogeneity of selected populations of cattle. Thus, as a result of studying the genetic structure of animals of two breeds of dairy cattle and their crossbreeds by intermicrosatellite DNA loci, their individual polymorphism was revealed. The amplification products have significant variations depending on the primer used. Primers ISSR-1 and ISSR-2 were the most informative for the analysis of cattle DNA polymorphism.

From Missionary Translation to Local Theological Inquiry: A Narrative History of the Lisu Bible

2019 ◽  
Vol 25 (2) ◽  
pp. 202-219
Author(s):  
Aminta Arrington
Keyword(s):  
Minority Group ◽  
Local Leadership ◽  
Bible Translation ◽  
Narrative History ◽  
History Of ◽  
Christian Practices ◽  
Village Elders ◽  
The Village ◽  
The Waves ◽  
Bible Reading

The Lisu are a largely Christian minority group in south-west China who, as an oral culture, express their faith more through a set of Christian practices done as a group and less through bible reading as individuals. Even so, the Lisu practice of Christianity specifically, and Lisu culture more generally, was profoundly impacted by the written scriptures. During the initial evangelisation of the Lisu by the China Inland Mission, missionaries created a written script for the Lisu language. Churches were constructed and organised, which led to the creation of bible schools and the work of bible translation. In the waves of government persecution after 1949, Lisu New Testaments were hidden away up in the mountains by Lisu Christians. After 1980, the Lisu reclaimed their faith by listening to the village elders tell the Old Story around the fires and reopening the churches that had been closed for twenty-two years. And they reclaimed their bible by retrieving the scriptures from the hills and copying them in the evening by the light of a torch. The Lisu bible has its own narrative history, consisting of script creating, translating, migrating, and copying by hand. At times it was largely influenced by the mission narrative, but at other times, the Lisu bible itself was the lead character in the story. Ultimately, the story of the Lisu bible reflects the Lisu Christian story of moving from missionary beginnings to local leadership and, ultimately, to local theological inquiry.

From the History of the Public Library in the Village Tagai of Simbirsk District in Simbirsk Province

2010 ◽  
pp. 102-106
Author(s):  
Valentina M. Patutkina
Keyword(s):  
Public Library ◽  
Library History ◽  
The Public ◽  
Ulyanovsk Region ◽  
History Of ◽  
In The Beginning ◽  
The Village

The article is dedicated to unknown page in the library history of Ulyanovsk region. The author writes about the role of Trusteeship on people temperance in opening of libraries. The history of public library organized in the beginning of XX century in the Tagai village of Simbirsk district in Simbirsk province is renewed.

MASJID BESAR RAJA HAJI ABDUL GHANI PULAU BURU KEPULAUAN RIAU

2018 ◽  
Vol 3 (2) ◽  
Author(s):  
Nurman Kholis
Keyword(s):  
Qualitative Research ◽  
Research Methods ◽  
19Th Century ◽  
Qualitative Research Methods ◽  
District Level ◽  
History Of ◽  
Chinese Buddhists ◽  
The Right ◽  
The 19Th Century ◽  
Level Government

Abstract. Many Muslims in the Riau Islands do not know the history of the development of Islamic theory from the center of power to spread to various corners. This is as the existence of the Great Mosque of Raja Haji Abdul Ghani (MBRHAG) on Buru Island, Karimun. Thus, to uncover the existence of this mosque, qualitative research methods are used so that history, architecture, and socio-religious functions can be known. Based on the results of the study it was concluded that the establishment of MBRHAG was initiated by Raja Haji Abdul Ghani. He was the first Amir (sub-district level government) of the kingdom of Riau-Lingga on Buru Island, in the 19th century. The architecture is a Chinese. Therefore, on the right side of this mosque is around 200 m, there is also the Sam Po Teng Temple and the Tri Dharma Dewa Bumi. Thus, the close location of the mosque with Chinese and Confucian worship houses's shows a harmonious relationship between Malay Muslims and Chinese Buddhists. In fact, in the continuation of this relationship there was information that a Chinese Buddhist had joined a Muslim friend to fast for half a month of Ramadan.Keywords: Mosque, Malay Muslims, Chinese Buddhists/Confucians, Harmonious RelationsAbstrak. Umat Islam di Kepulauan Riau banyak yang tidak mengenal sejarah perkembangan ajaran Islam dari pusat kekuasaan hingga tersebar ke berbagai pelosok. Hal ini sebagaimana keberadaan Masjid Besar Raja Haji Abdul Ghani (MBRHAG) di Pulau Buru, Karimun. Dengan demikian, untuk mengungkapkan keberadaan masjid ini digunakan metode penelitian kualitatif  agar dapat diketahui sejarah, arsitektur, dan fungsi sosial keagamaannya.  Berdasarkan hasil penelitian diperoleh kesimpulan bahwa pendirian MBRHAG diprakarsai oleh Raja Haji Abdul Ghani. Ia adalah Amir (pemerintah setingkat kecamatan) pertama kerajaan Riau-Lingga di Pulau Buru, pada abad ke-19. Adapun arsitekturnya adalah seorang Tionghoa. Karena itu, di sebelah kanan masjid ini sekitar 200 m juga terdapat Kelenteng Sam Po Teng dan cetya Tri Dharma Dewa Bumi. Dengan demikian, dekatnya lokasi masjid dengan rumah ibadah umat Tionghoa dan Khonghucu ini menunjukkan hubungan yang harmonis antara muslim Melayu dengan Budhis Tionghoa. Bahkan, dalam kelangsungan hubungan ini terdapat informasi seorang Buddhis Tionghoa pernah ikut temannya yang beragama muslim untuk berpuasa selama setengah bulan Ramadhan.Kata Kunci: Masjid, Muslim Melayu, Buddhis/Khonghucu Tionghoa, Hubungan Harmonis

FORMATION AND DEVELOPMENT OF RURAL ARCHITECTURE IN THE FORMER KETSKAYA VOLOST

2020 ◽  
Vol 22 (2) ◽  
pp. 9-28 ◽  
Author(s):  
E. V. Sitnikova
Keyword(s):  
Cultural Heritage ◽  
18Th Century ◽  
Scientific Methods ◽  
Architectural Analysis ◽  
Tomsk Region ◽  
History Of ◽  
The Rich ◽  
Rural Architecture ◽  
The Village ◽  
Rich History

The article considers the historical and cultural heritage of villages of the former Ketskaya volost, which is currently a part of the Tomsk region. The formation of Ketsky prison and the architecture of large settlements of the former Ketskaya volost are studied. Little is known about the historical and cultural heritage of villages of the Tomsk region and the problems of preserving historical settlements of the country.The aim of this work is to study the formation and development of the village architecture of the former Ketskaya volost, currently included in the Tomsk region.The following scientific methods are used: a critical analysis of the literature, comparative architectural analysis and systems analysis of information, creative synthesis of the findings. The obtained results can be used in preparation of lectures, reports and communication on the history of the Siberian architecture.The scientific novelty is a study of the historical and cultural heritage of large settlements of the former Ketskaya volost, which has not been studied and published before. The methodological and theoretical basis of the study is theoretical works of historians and architects regarding the issue under study as well as the previous  author’s work in the field.It is found that the historical and cultural heritage of the villages of the former Ketskaya volost has a rich history. Old historical buildings, including religious ones are preserved in villages of Togur and Novoilinka. The urban planning of the villages reflects the design and construction principles of the 18th century. The rich natural environment gives this area a special touch. 

Gene mutation and drug resistance of M. tuberculosis in the patients followed up in the city of Moscow

2020 ◽  
Vol 97 (12) ◽  
pp. 34-44
Author(s):  
M. A. Krasnova ◽  
E. M. Belilovsky ◽  
S. E. Borisov ◽  
A. A. Khakhalina ◽  
Yu. D. Mikhaylova ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Statistical Significance ◽  
Molecular Genetic ◽  
Previous Treatment ◽  
Repeated Treatment ◽  
Phenotypic Resistance ◽  
Therapy Regimen ◽  
Treatment History ◽  
History Of ◽  
Relationship Of

The article describes a retrospective study of the results of microbiological and molecular genetic tests of 685 M. tuberculosis cultures isolated from 685 adult tuberculosis patients registered for dispensary follow-up in Moscow in 2014.The following was identified during the study: phenotypic drug resistance (FDR) of MTB to rifampicin, isoniazid, fluoroquinolones, kanamycin, amikacin, and capreomycin in groups of patients with different treatment history; the frequency of FDR to the above anti-tuberculosis drugs in strains with mutations being drug resistance markers; the frequency of various mutations in case of FDR of mycobacteria in the patients from different groups; the relationship of FDR or the presence of a particular mutation with various characteristics of the patients and their treatment history.The history of previous treatment was determined as statistical significance to provide the greatest influence on the spread of drug resistant MTB: patients undergoing repeated treatment had FDR more often and also a much more pronounced variety of mutations being markers of FDR to certain anti-tuberculosis drugs.The results of the study showed that the detection of genetic mutations in MBT associated with FDR was a reliable tool for predicting phenotypic resistance and should be used as the main method for selecting anti-tuberculosis drugs when compiling the etiotropic therapy regimen.

COMBINING ORAL TRADITIONS AND BAYESIAN CHRONOLOGICAL MODELING TO UNDERSTAND VILLAGE DEVELOPMENT IN THE GULF OF PAPUA (PAPUA NEW GUINEA)

Radiocarbon ◽  
2021 ◽  
pp. 1-21
Author(s):  
Chris Urwin ◽  
Quan Hua ◽  
Henry Arifeae
Keyword(s):  
Papua New Guinea ◽  
New Guinea ◽  
Oral Traditions ◽  
Long Distance ◽  
Spatial History ◽  
The Past ◽  
History Of ◽  
Gulf Of Papua ◽  
The Village

ABSTRACT When European colonists arrived in the late 19th century, large villages dotted the coastline of the Gulf of Papua (southern Papua New Guinea). These central places sustained long-distance exchange and decade-spanning ceremonial cycles. Besides ethnohistoric records, little is known of the villages’ antiquity, spatiality, or development. Here we combine oral traditional and 14C chronological evidence to investigate the spatial history of two ancestral village sites in Orokolo Bay: Popo and Mirimua Mapoe. A Bayesian model composed of 35 14C assays from seven excavations, alongside the oral traditional accounts, demonstrates that people lived at Popo from 765–575 cal BP until 220–40 cal BP, at which time they moved southwards to Mirimua Mapoe. The village of Popo spanned ca. 34 ha and was composed of various estates, each occupied by a different tribe. Through time, the inhabitants of Popo transformed (e.g., expanded, contracted, and shifted) the village to manage social and ceremonial priorities, long-distance exchange opportunities and changing marine environments. Ours is a crucial case study of how oral traditional ways of understanding the past interrelate with the information generated by Bayesian 14C analyses. We conclude by reflecting on the limitations, strengths, and uncertainties inherent to these forms of chronological knowledge.

scholarly journals Evaluation of DNA Extraction Methods Developed for Forensic and Ancient DNA Applications Using Bone Samples of Different Age

Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 146
Author(s):  
Catarina Xavier ◽  
Mayra Eduardoff ◽  
Barbara Bertoglio ◽  
Christina Amory ◽  
Cordula Berger ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Ancient Dna ◽  
Extraction Method ◽  
Fragment Size ◽  
Molecular Genetic ◽  
Extraction Methods ◽  
Degraded Dna ◽  
Size Analysis ◽  
Dna Fragments ◽  
Dna Amount

The efficient extraction of DNA from challenging samples, such as bones, is critical for the success of downstream genotyping analysis in molecular genetic disciplines. Even though the ancient DNA community has developed several protocols targeting small DNA fragments that are typically present in decomposed or old specimens, only recently forensic geneticists have started to adopt those protocols. Here, we compare an ancient DNA extraction protocol (Dabney) with a bone extraction method (Loreille) typically used in forensics. Real-time quantitative PCR and forensically representative typing methods including fragment size analysis and sequencing were used to assess protocol performance. We used four bone samples of different age in replicates to study the effects of both extraction methods. Our results confirm Loreille’s overall increased gain of DNA when enough tissue is available and Dabney’s improved efficiency for retrieving shorter DNA fragments that is beneficial when highly degraded DNA is present. The results suggest that the choice of extraction method needs to be based on available sample, degradation state, and targeted genotyping method. We modified the Dabney protocol by pooling parallel lysates prior to purification to study gain and performance in single tube typing assays and found that up to six parallel lysates lead to an almost linear gain of extracted DNA. These data are promising for further forensic investigations as the adapted Dabney protocol combines increased sensitivity for degraded DNA with necessary total DNA amount for forensic applications.

Sign in / Sign up

Export Citation Format

Share Document