scholarly journals EVALUATION OF DAIRY PRODUCTIVITY AND REPRODUCTIVE ABILITY OF UKRAINIAN BLACK-AND-WITE AND RED-AND-WITE MILK COWS DEPENDING ON THE SELECTIONAL DIRECTION OF HOLSTEIN BREED

2017 ◽  
Vol 54 ◽  
pp. 178-184 ◽  
Author(s):  
V. V. Machulnyi ◽  
S. N. Pokryshcuk ◽  
A. A. Sorokin

Formation of high-yielding herds is a rather long breeding process (at least 10–12 years), during which animals of the 3rd–4th generation appear. During this period, it is necessary to preserve a certain scientifically justified direction of selection to perform the tasks, the embodiment of which in life is possible only in the presence of a long-term plan of breeding work. Selection of cows for milk productivity primarily depends on the degree of influence on this feature of the main genetic and environmental factors, taking into account that you can significantly improve the desired attributes. The purpose of our research was to assess productive and reproductive animal characteristics of Ukrainian red- and black-and-white dairy breeds, search of the most effective breeding methods for improving the reproductive function of cows and heifers. Indicators of economically useful signs of the animals under investigation are calculated according to the data of the primary zootechnical account according to the generally accepted methods of biometric analysis. In the course of the work the genealogical, population, genetic and mathematical methods of studying patterns of inheritance, variability, repeatability, estimation of the genotype of animals and other classical methods of research were used. According to the results of our research, it is evident that the best milk production is characterized by the animals obtained by using the Holstein breed of North American breeds. A well-defined difference of 234 kg is observed in the cows of the Ukrainian black-and-white milk breed, and in the red- and-white peers there is an insignificant difference between the groups. That indicates that the high-level animals of the Holstein breed do not fully realize their genetic potential by productivity. It should be noted that the bloodiness of the North American selection of Holstein breeds in the genotypes of both breeds, in contrast to the diet, had a negative effect on the protein content of milk (milk protein content decreased by 0.5–0.9% In comparison with the animals of the desirable type of daughter from the European bulls of the Holstein breed in the group of Ukrainian black- and red- and-white dairy breeds were significantly superior to or were at the level of the animals of the desired type for fatty milk and milk protein content, unlike the daughters from the bulls of the North American breeding direction. Animals derived from the bulls of the North American breeding Holstein breed had the longest service period 166.1 days, which is 36.4 days more than that of the animals of the same breed, but from the bulls of European breeding Holstein breed (P > 0,05). It was also characterized by a longer give birth period, which amounted to 445.4 days, prevailing at the same time of first-born cows with European genetics of Holstein breed for 36 days (P > 0,05). According to the indicator of reproductive ability of animals of Ukrainian black-and-white milk breeds holsteinized by the European type of breeding Holstein breed have a better result of 0.89 in contrast to the peers  holsteinized by the North American type of selection 0,86 (P > 0,05). In the comparative analysis of the correlation of reproductive ability of cows with different genotypic groups of both breeds, the cows with the European type of selection of the Holstein breed are preferable to the parameters of the animals of the desired type. Conclusions. According to the results of the evaluation of milk productivity, the preference is given to the animals obtained from the bulls of the Holstein breed of North American breeding by quantitative indices but this genetics negatively affected the protein content of milk. Compared to the animals of the desired type, – then both breeding directions are considerably inferior to the parameters of the animals of the desired type, but on qualitative indicators of milk productivity the cows from the bulls of European breeding Holstein breed prevail and their peers with the North American selection of Holstein breed and animals of the desired type. According to the performance of the reproductive ability of the daughter of the North American bulls Holstein breed had the longest service- and between the give birth period, and shorter dry period. According to the indicator of reproductive ability, dominated cows by the European type of breeding Holstein breed.

Author(s):  
Л.Р. ЗАГИДУЛЛИН ◽  
Р.Р. ШАЙДУЛЛИН ◽  
Т.М. АХМЕТОВ ◽  
С.В. ТЮЛЬКИН ◽  
А.Б. МОСКВИЧЕВА

Изучена взаимосвязь аллельных вариантов генов пролактина и соматотропина с показателями молочной продуктивности коров черно-пестрой породы по 1 и 3 лактации. У первотелок, как и у полновозрастных животных,  наиболее высокий удой (4642 и 6240 кг), количество молочного жира (174,1 и 215,0 кг) и молочного белка (149,0 и 182,5 кг) достигнуты в группе с генотипом PRL АА. По массовой доле жира и белка в молоке коровы с генотипом PRL ВВ достоверно превосходили животных с генотипом PRL АА на 0,12% (Р<0,05) и 0,05% (Р<0,05) соответственно. По гену соматотропина наибольший уровень молочной продуктивности выявлен у коров с генотипом GH LL при достоверном преимуществе над GH VV у первотелок по удою на 439 кг (Р<0,01), выходу молочного жира — на 13,6 кг (Р<0,001), выходу молочного белка — на 15,3 кг (Р<0,01). У полновозрастных коров превышение над остальными группами было только по удою — на 219—548 кг (Р<0,05). Наибольшая жирномолочность характерна животным с генотипом GH VV по 1 лактации — 3,83% и по 3 лактации — 3,82%, а лучшая белковомолочность — коровам, имеющим аллель V гена GH:  3,21 и 3,23% соответственно. Полученные данные свидетельствуют о более высоком уровне молочной продуктивности у коров с генотипом PRL АА и GH LL, но лучшая жирномолочность и белковомолочность отмечены у особей, имеющих в своем генотипе аллели В гена PRL и V гена GH. The interrelation of allelic variants of prolactin and somatotropin genes with indicators of milk productivity of black-and-white cows for the 1st and 3rd lactation was studied. The highest milk yield (4642 and 6240 kg) amount of milk fat (174.1 and 215.0 kg) and milk protein (149.0 and 182.5 kg) were in the group with the PRL AA genotype in first-calf heifers as well as in full-age animals. In terms of the mass fraction of fat and protein of milk, the cows with the PRL BB genotype significantly exceeded animals with the PRL AA genotype by 0.12% (P<0.05) and 0.05% (P<0.05), respectively. According to the somatotropin gene, the highest level of milk productivity was revealed in cows with the GH LL genotype with a significant advantage over GH VV in first-calf heifers in milk yield by 439 kg (P<0.01), milk fat yield by 13.6 kg (P<0.001), milk protein yield by 15.3 kg (P<0.01). The excess over the other groups in full-age cows was only in terms of milk yield that was by 219-548 kg (P<0.05). The highest protein content of milk is a characteristic of animals with the GH VV genotype, 3.83% is for the 1st lactation and 3.82% is for the 3rd lactation, and the best protein content of milk in cows with V allele of the GH gene is 3.21 and 3.23%, respectively. The obtained data  indicated a higher level of milk productivity in cows with the PRL AA and GH LL genotypes but the best fat and milk content was noted in cows with alleles B of the PRL gene and V of the GH gene in their genotype.


2019 ◽  
Vol 49 (4) ◽  
pp. 58-64
Author(s):  
A. G. Kolchev

The results of the study of milk productivity and lactation rates of fi rst-calf cows are presented. The research into fi rst-calf cows of black-andwhite Holstein breed, belonging to sire lines of Wis Ideal 1013415 and Refl ection Sovereign 198998 was conducted in 2018 in Novosibirsk region. The number of cows under study was 64, the period of the study covered 305 days of lactation. Monitoring of milk production, milk fl ow intensity, fat and protein content was carried out monthly. The research materials were analyzed for groups of cows depending on their sire line and productivity for single and daily milk yield, fat and protein content, and dynamics of milk ejection for the fi rst 3 minutes of milking. The highest daily milk yield in both lines was observed in the second month of lactation. In the fourth and fi fth months of lactation, the average daily milk yield of Refl ection Sovereign cows was signifi cantly higher (р < 0.001) than that of their peers of the Vis Ideal line, by 1.3 kg. During the lactation period, the productivity of the Vis Ideal and Refl ection Sovereign cows was almost the same: milk yield – 8296 and 8220 kg, fat content – 4.02 and 4.06%, protein – 3.36 and 3.35% and milk fl ow intensity – 2.70 and 2.78 kg/min, respectively. In the second and third months of lactation, cows of both lines had a decrease in the content of fat, protein and intensity of milk fl ow with a gradual increase in these indices in the fourth and fi fth months of lactation. First-calf cows of the black-and-white breed of Canadian selection of different sire lines showed a high level of milk productivity and proved to be suitable for further breeding work.


Author(s):  
N. P. Babik

Recently, in the breeding and commodity farms of our country can be observed a rapid decrease in the duration of productive use dairy cattle. A considerable amount of scientific researches are conducted on the effects of hereditary and environmental factors on productive indicators longevity, researches are done on various ways to increase durability productive use of dairy cattle. In this aspect, reproductive ability of cows is important, which must be taken into account while individual estimation for the next selection of the most valuable animals that combine high milk productivity with high fertility. Therefore, the goal of our research was to study the productive longevity of dairy cows depending on the duration of their first service period and find the optimal duration of this indicator for a particular breed. The research was carried out on cows of Holstein, Ukrainian Black-and-White and Red-and-White breeds. Retrospective analysis of duration and the effectiveness of life-time use of cows was carried out according to the method Yu P. Polupan (2010). It was found that average duration of the first service-period of cows was 167.7 days on the studied Holstein populations, 180.3 – on the Ukrainian Black-and-White and Ukrainian Red-and-White – 142.1 days. The longest duration of life, productive use, lactations and the highest milk yields had cows with the duration of the first service period of 121–150 days among the Holstein breed, 151–180 – Ukrainian Black-and-White and 91–120 days and Ukrainian Red-and-White. The worst, by the indicators of productive longevity, were individuals with 60 days and no more of the first service period. Correlation analysis showed the different level of the connection between the duration of the service period animals and indicators of their lifelong use, which in most cases had a reverse nature. This kind of connection indicates that with the extension of the duration of service period of cows, the duration of their productive use and lifetime productivity decreases. In Holstein breed animals, the correlation coefficients between the above named indicators ranged from -0.462 to 0.370, Ukrainian Black-and-White – from -0.113 to 0.144 and Ukrainian Red-and-White – from 0.279 to 0.149. Strength of influence of the duration of the first service-period on life duration, productive use and lactation of cows, depending on the breed, was 12.5–36.5%, for lifetime productivity – 9.7–34.6 and on the one day of life, productive use and lactation – 11.3–35.9%.


2018 ◽  
Vol 56 ◽  
pp. 50-64 ◽  
Author(s):  
N. P. Mazur ◽  
Ye. I. Fedorovych ◽  
V. V. Fedorovych

A fundamental genetic improvement of domestic dairy breeds is carried out by using the gene pool of the best foreign breeds, in particular Holstein. This approach has greatly improved the milk productivity of cows, but has led to a significant deterioration in reproduction, longevity, product quality, general animal health etc. Taking into account that the increase of the milk productivity of cows leads to a shortening of their use, this problem will only become aggravated over time. Therefore, it is now necessary to direct scientific research into a comprehensive assessment of animals taking into account the signs of lifelong productivity. In view of the above, the purpose of our research was to study the economic utility signs of dairy cows and their relationship with productive longevity. The research was conducted on cows of Holstein (n = 2902), Ukrainian Black- (n = 14876) and Red-and-White (n = 2176) dairy breeds. To characterize the economic useful signs of animals of the studied breeds based on the materials of the primary zootechnical and breeding records, weighed growth of animals, reproductive capacity, milk productivity and duration and efficiency of lifetime use were studied. It was established that heifers of investigated dairy breeds were characterized by a moderate intensity of growth of live weight, as evidenced by average daily increments from birth to 18 months of age: in animals of Holstein breed – 644, Ukrainian Black-and-White milk breed – 641 and Ukrainian Red-and-White dairy breed 692 g. The first fruitful insemination of heifers of the Holstein breed occurred on average at the age of 19.1, Ukrainian Black and Red-and-White dairy breeds - 20.4 and 20.8 months, while their live weight at that was 405.3; 414.3 and 438.5 kg respectively. The milk yield of the Holstein breed cows, depending on the lactation, was 4846–7920 kg, the fat content in milk was 3.63–3.74% and the amount of milk fat was 181.2–279.7 kg, the Ukrainian Black-and-White milk breed was 4008–6317 kg, 3.63–3.70% and 148.6–228.8 kg respectively, while Ukrainian Red-and-White milk is 4578–6592 kg, 3.74–3.87% and 177.2–245.9 kg. The cows of the given breeds were used in herds only 2.32–2.50 lactation. The highest life milk yield was noted in animals of the Holstein breed (18,669 kg), and the lowest (14,940 kg) in the cows of the Ukrainian Red-and-White breed. Correlation analysis of economic characteristics of dairy cows with indicators of their productive longevity confirms the possibility of conducting an indirect predictive selection of animals in order to form high-yielding herds with long-term economic use. Among the studied features, the greatest predictive value (P < 0.001) for the indicators of life expectancy, productive use, lactation, the number of lactation per life, life yield and lifetime of milk fat have yield of cows for the first (r = -0.217 – +0.205) and the best lactation (r = +0.061 – +0.609). An intermediary predictive selection of animals can also be carried out according to the duration of their first service period (r = -0.462 – +0.106) and live weight during growing season (r = -0.286 – +0.126). It was established that live weight at the first insemination and the first calving significantly influenced the indicators of life yields, economic use and lactation of animals, as evidenced mainly by higher and reliable values of correlation coefficients between these indices. It should also be noted that the correlation coefficients between the live weight of cows in the first calving and the duration of life, productive use, lactation and the number of lactations in life were somewhat higher but negative values (r = -0,130 – -0,070), compared with the between live weight at the time of first insemination and the above indicators of longevity of animals (r = -0,037 – +0,094). This suggests that the effect of live weight on the first calving of animals on their longevity was somewhat higher than the effect of live weight at the first insemination. Our data show that the selection of cows by the age of the first calving and the duration of the first lactation is not significant, since there is practically no link between these features and the indicators of productive longevity.


2017 ◽  
Vol 54 ◽  
pp. 146-156
Author(s):  
T. M. Suprovych ◽  
M. P. Suprovych ◽  
R. V. Kolinchuk

Introduction. The main direction of increasing the productivity of milk is to increase the proportion of heredity of the Holstein breed in the genotype of cows. Industrial breeds in Ukraine are improving due to the increase in the Holstein inheritance in the genotype of cows. The "holsteinization" of the most widespread domestic Black-and-White diary breed is intensively conducted. Currently, the percentage of heredity from Holstein is 90% or more. The negative effect of "holsteinization" appeared in reducing the resistance of animals to diseases that led to the spread of necrobacterial pathology. The control of the spread of necrobacteriosis can be based on genetic markers. Important markers can be the allele of the BoLA-DRB3.2 gene responsible for the formation of adaptive immunity. Due to the ambiguity of the results of "holsteinization", the following tasks were solved: To study the genetic structure of the herd for the BoLA-DRB3.2 gene at the beginning of the "holsteinization" and now. To compare the detected genetic structures with the alleles spectrum of North American Holstein and identify quantitative and qualitative changes in the structure of the herd genotype. To determine the effect of "holsteinization" on the dynamics of milk production and the state of morbidity by necrobacteriosis. Materials and methods of research. Comparison of alleles of population of the Ukrainian Black-Pied Dairy (UBPD) breed and Holstein breed was conducted to detect the consequences of "holsteinization" on milk yield and incidence of necrobacteriosis. The data of the allelic polymorphism of the BoLA-DRB3.2 gene of the UBPD10 (2010, n = 162), UBPD15 (2015, n = 114) and two Holstein populations of the USA and Canada were collected. The allelic spectrum was determined by the PCR-RFLP method. The amplification of the BoLA-DRB3.2 gene was performed using 2-step PCR with the use of primers HLO-30, HLO-31 and HLO-32 and allele-specific PCR. Restriction analysis was performed with endonuclease RsaI, HaeIII, BstYI (XhoII). Restriction fragments were separated by electrophoresis in 4% agarose gel. Counting of allele frequencies was performed taking into account the number of homozygotes and heterozygotes found for the corresponding alleles. To determine the phylogenetic relationships between the populations of the studied herds, genetic distance and genetic similarity were determined by the M. Nei method. Individual dairy productivity of cows was estimated for all lactation (regardless of its duration). Average milk yields were determined as the total volume of milk produced divided by the number of dairy cows. Results and discussion. The breeding measures carried out led to the accumulation of alleles characteristic of the Holstein breed. For Holstein, there are eight alleles with a frequency of more than 4%. It is alleles *03, *07, *08, *11, *16, *22, *23, *24. A high degree of consolidation of weighty alleles can be outlined. In total they occupy 84,6% of allele spectrum of the population. Consolidation of such alleles in the herd of the Ukrainian Black-and-White diary breed is much lower - only 52.2%, although it increased by 6.2% over 5 years. Alleles *10, *13 and *28 are "weighty" for the Ukrainian Black-and-White diary breed, but they are almost non-existent in Holsteins. The genetic similarity of the herd UBPD15 and Holstein increased by ΔI = 0,085, and the genetic distance between the herds of the UBPD increased by ΔD = 0,085 for 5 years. The comparison of the allele spectrum of Holstein and the Ukrainian Black-and-White diary breed shows both the accumulation and the elimination of alleles associated with high productivity. The largest consolidation is typical for alleles *24 (+ 6.75%) and *16 (+ 4.65%). The frequency of "milk" alleles *22 and *08 decreased, respectively, by 4.14 and 1.27%. Alleys, which cause low milk productivity, have the following dynamics: * 23 + 2.53%, *11 – 0.67 and *28 – 0.26. The accumulation of alleles *16 and *23 (7.18%) was found that are associated with predisposition to necrobacteriosis and elimination of *03 and *22 alleles (4.75%) that influence on this disease. Conclusions. It is determined that the role of alleles characteristic for Holstein is increasing in the the Ukrainian Black-and-White diary herd. Breeding measures for holsteinization are conducted in the right direction. There is accumulation of alleles associated with high milk productivity and predisposition to necrobacteriosis. It positively affects the growth of milk production and negatively affects the incidence of necrobacteriosis.


2016 ◽  
Vol 52 ◽  
pp. 6-12 ◽  
Author(s):  
M. V. Gladiy ◽  
G. S. Kovalenko ◽  
S. V. Priyma ◽  
G. A. Holyosa ◽  
A. V. Tuchyk ◽  
...  

The main goal of dairy breeds selection should be improving breeding and productive qualities of animals under modern conditions. The majority of farms, using native breeds to produce milk, has created optimal conditions for keeping and feeding, selection and matching, growing of replacements etc. Further improvement of created native dairy breeds for economically useful traits occurs at total use of purebred Holstein bulls (semen) of foreign selection. In order to realistically assess milk productivity (milk yield, fat content in milk and fat yield) of Ukrainian Black-and-White and Red-and-White Dairy cows should be conducted a comparative analysis of Holstein cows under the same conditions of feeding and keeping. It was established that Ukrainian Red-and-White Dairy cows were characterized by the highest milk yields for 305 days of all lactations, taken into account, the among three investigated breeds. Their milk yield during the first lactation was 5933 kg of milk, during the second – 6393 kg, the third – 6391 kg and during higher lactation – 6650 kg. Ukrainian Black-and-White Dairy cows were second by milk yield (except for the second lactation), during the first lactation – 5932 kg of milk, the third – 6462 kg and higher – 6541 kg, and Holstein cows were third, during the first lactation – 5794 kg of milk, the second – 6381 kg, the third – 6335 kg and higher – 6469 kg. The fat content was almost the same and varied within 3.49-3.58% in milk of Ukrainian Red-and-White Dairy cattle, 3.50-3.60% in milk of Ukrainian Black-and-White Dairy cattle and 3.50-3.56% in Holsteins’ milk. The difference between the breeds was within 0.01-0.04%. All the investigated breeds had predominance in fat yield for three lactations over standards of these breeds: Ukrainian Red-and-White Dairy cows from 75.1 to 93.4 kg, Ukrainian Black-and-White Dairy cows – 75.1-89.0 kg respectively and Holstein cows – 41.9-60.2 kg. It was found different level of positive correlation between milk yield and fat yield in all the cases and high correlation (r = 0.604-0.921, P < 0.001) in five cases (41.7%) Negative correlation coefficients indicate that selection of animals to higher milk yield in the herd will decrease the second trait – fat content in milk. Positive and highly significant correlation between milk yield and fat yield indicates that selection of cows in the herd to higher milk yields will increase fat yield. It was revealed that bulls were among the factors impacted the milk productivity (milk yield, fat content, fat yield) of three investigated breeds. So, the force (η²x) of father’s impact on milk yield was15.4-47.9%, fat content – 22.0-43.4% and fat yield – 14.9-47.7% taking into account a lactation and a breed. The force of lines impact (η²x) was second; it was on milk yield 6.1-24.5%, fat content – 4.1-17.1 and fat yield – 5.8-23.5%. The force of breeds impact (η²x) was last; it was on milk yield 0.3-2.9%, fat content – 0.2-0.3% and fat yield – 0.6-2.7%. So, the comparative studies of milk productivity of Ukrainian Red-and-White and Black-and-White Dairy cattle with Holsteins indicate that under similar conditions of feeding and keeping, these native breeds can compete with Holstein cattle. The milk yield for 305 days of higher lactation was 6650 kg of milk in Ukrainian Red-and-White Dairy cows, 6541 kg in Ukrainian Black-and-White Dairy cows and 6469 kg in Holsteins. It was found the inverse correlation r = -0.025-0.316 between milk yield and fat content in milk in most cases. Selection and matching of animals in the herd should be carried out simultaneously on these traits. It was found positive repeatability of milk yields between the first and second, the third and higher lactations (rs = 0.036-0.741), indicating the reliability of forecasting increase in milk productivity during the next lactations in all herd. Bulls have the greatest impact (η²x) on milk productivity among the factors taken into account: milk yield – 15.4-47.9%, fat content in milk – 22.0-43.4% and fat yield – 14.9-47.7%.


Author(s):  
A. Zheltikov ◽  
N. Kostomakhin ◽  
D. Adushinov ◽  
O. Zaiko ◽  
V. Dementev ◽  
...  

The characteristic of milk productivity of cows of Holstein and Simmental breeds in LLC “Sibirskaya Niva” in the Maslyaninsky district in the Novosibirsk region has been given. It has been revealed that under the natural and climatic environments of the Western Siberia, when creating favorable conditions for feeding and housing for cows of Holstein and Simmental breeds have sufficiently high milk productivity. It has been found that Holstein first-calf heifers surpassed their Simmental herdmates by 959 kg in milk yield for the first 305 days of lactation under the same conditions of housing and feeding, by 3,1 kg in daily milk yield, by 38,2 kg in milk fat yield, and by 28,3 kg in milk protein yield (P < 0,001). There were no statistically significant differences between animals of Holstein and Simmental breeds in terms of fat and protein content in milk, which amounted to 3,94 and 2,96 % in Holstein breed and 3,97 and 2,98 % in Simmental breed, respectively. Both breeds have shown high milk productivity during the first 3 lactations. Thus, the milk yield of Holstein cows for the first 3 lactation was 6475–9290 kg, Simmental cows it was 5516–7680 kg, fat content was 3,93–3,99 and 3,96–3,97 %, respectively. The protein content in the milk of cows of both breeds did not exceed 3,0 %. High variability has been found in milk yield, yield of milk fat and protein, the coefficient of milk content and duration of lactation, the lowest in the content of protein and fat in milk and live weight. The correlation coefficients between the fat and protein content in milk were generally positive, but did not exceed 0,28. Therefore, breeding in the herd of cows in LLC “Sibirskaya Niva” have to be conducted not only by fat content, but also by protein content in milk.


2020 ◽  
Vol 52 (11) ◽  
pp. 1191-1201
Author(s):  
Ling Chen ◽  
Rongfu Tian ◽  
Huilin Zhang ◽  
Xiaolin Liu

Abstract NFκB1/p105 is the critical member of the NFκB family which can suppress inflammation, ageing, and cancer when p50/p50 homodimer is formed. Currently, the research about the role of NFκB1/p105 during cow mastitis is limited. Here, we analyzed the correlation of six single-nucleotide variants of the NFκB1 gene with somatic cell count, milk yield, milk fat content, and milk protein content in 547 Chinese Holstein cows, and explored the mRNA expression profiles of the NFκB family and ubiquitin ligases (βTrCP1, βTrCP2, KPC1, KPC2) in LPS-induced bovine mammary epithelial cells (MECs) by transcriptome-Seq. The association analysis showed that cows with SNV2-TT and SNV6-CC in the NFκB1 gene had significantly higher milk protein content (P &lt; 0.05), while cows with SNV5-TT in the NFκB1 gene had significantly lower somatic cell score (SCS), but CC genotype at SNV5 locus was not detected in our Holstein cows. The transcriptome-Seq results demonstrated the mRNA expression of NFκB1 was increased and peaked at 4 h post-induction, while the mRNA expressions of both KPC1 and BCL3 that promote the anti-inflammation function of NFκB1/p105 were decreased in LPS-induced bovine MECs. TNFAIP3, an inhibitor of both degradation and processing of p105 precursor, was markedly increased by more than 3 folds. Furthermore, bta-miR-125b which targets at the 3ʹUTR of TNFAIP3 was reduced by 50%. These results indicated that SNV5-TT of the NFκB1 gene with lower SCS may be an anti-mastitis genotype that could cope with infection more efficiently in Chinese Holstein cows. In addition, the anti-inflammation role of NFκB1/p105 seemed to be inhibited in LPS-induced-bovine MECs because the formation of the p50/p50 homodimer was arrested. This study provides a new perspective to understand the inflammatory mechanism in dairy mastitis.


2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 263-264
Author(s):  
Sergey Anikin ◽  
Natalia Shemuranova ◽  
Natalia Garifullina ◽  
Andrey Filatov ◽  
Alexander Sapozhnikov

Abstract The aim of the research was to establish the effectiveness of the use of the feed additive “Lamarin Saldonum.” The feed additive contains Japanese kelp, milk thistle, to increase the milk productivity during the milking period. The studies were conducted on 40 cows of the black-and-white Holstein breed at the age of 2–4 lactation and the amount of milk for the last lactation not less than 7,500 kg. The cows were divided into 4 groups (GE1, GE2, GE3, GC) of 10 heads in each. Cows GE1, GE2, GE3 received 30 days before calving daily feed additive in doses of 0.1, 0.2, 0.3 g/kg, respectively, for 60 days. In the GC animals didn’t receive the feed additive (control group). Control milking was performed every 10 days. Using of feed additive in the ration increased milk productivity. In the 1st month of lactation, the average daily amount of milk in GC was 24.00±0.93 kg, which is 7.4%, 19.6% (P &lt; 0.01), 7.4% less than in GE1, GE2 and GE3, respectively. In the 2nd month, the highest average daily amount of milk in GE2 and GE3, which is 13.4% (P &lt; 0.01) and 4.6% more than the GC, respectively. In the 3rd month, the average daily amount of milk in GE1, GE2, and GE3 was 30.22±2.44 kg, 39.57±2.68 kg, 35.11±2.59 kg, respectively, which is 6.8%, 39.9% (P &lt; 0.01), 24.1% more than in GC. The mass fraction of fat in milk in animals GE1, GE2, GE3 was 0.2–0.92%(P &lt; 0.01) higher than in GC. In the second month of lactation, the mass fraction of protein in GE2 was higher by 0.24% (P &lt; 0.01), in GE3–by 0.14 (P &lt; 0.05) relative to GC. At the 3rd month of lactation, the protein content in milk was 0.29% higher in GE2 (P &lt; 0.001) and GE3-by 0.21% (P &lt; 0.001) than in GC.


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