ASSOCIATION OF THE ESR1 GENE WITH REPRODUCTIVE TRAITS OF SOWS OF LARGE WHITE AND MIRGOROD BREEDS

2016 ◽  
Vol 52 ◽  
pp. 150-158
Author(s):  
V. N. Balatsky ◽  
L. P. Grishina ◽  
A. M. Saenko ◽  
V. A. Vovk ◽  
P. A. Vaschenko
Keyword(s):  
Estrogen Receptor ◽  
Reproductive Traits ◽  
Large White ◽  
Dominant Component ◽  
Large White Breed ◽  
Additive Component ◽  
White Breed ◽  
Pvuii Polymorphism ◽  
The Impact ◽  
Estrogen Receptor 1

The speciality of the modern methodology in breeding is using the molecular information, received during genome analysis. This methodology can significantly accelerate the improvement of productivity traits and it is particularly useful in relation to the traits with low coefficient of inheritance while classic methods are not effective enough. The reproductive traits are one of the most important in pig farming, estrogen receptor 1 gene (ESR1) is involved in their control. Meanwhile, the use of ESR1 locus polymorphism in the marker-assisted selection needs to determine the extent of its association with the reproductive traits of animals in those populations where it is planned to conduct such selection. Implementation of marker-assisted selection in Large White and Mirgorod breeds for improving the reproductive traits is an actual task, but a necessary step in this work is the associative analysis. The purpose of the work is to research the association of polymorphisms of ESR1 locus with some reproductive traits of sows of Large White breed (ULW-1 and ULW-3 lines) and sows of Mirgorod breed. Materials and methods of research. Experimental groups: 1) the sows of Large White breed, ULW-3 line, bred in "Bahmutskiy Agrarian Union" farm, Donetsk region; 2) the sows of Large White breed, ULW-1 line, bred in “Stepne” farm, Poltava region; 3) the sows of Mirgorod breed, bred in «Named after Dekabristy» farm, Poltava region. All the experimental animals were previously genotyped on RYR1 gene and had RYR1CC genotype. The animals were genotyped on estrogen receptor 1 locus with aid of PCR-RFLP analysis on PvuII-polymorphic restriction site in the third intron of the gene – DNA marker for estrogen receptor 1 gene. Associations between genotypes and the studied traits were calculated using ANOVA in Excel 2007. Results. ULW-3 sows with ESR1BB genotype turned out to have 1.36 more piglets in a litter (analysing data from 2nd-4th farrows) comparing to animals with ESR1AA genotype. There is a tendency for bigger amount of newborn piglets in the heterozygotes animals than in sows with homozygous ESR1AA. A similar pattern appears in the 1st farrowing, the sows with ESR1BB and ESR1AB genotypes had the advantage in the total number of piglets at birth. In the experimental group of ULW-1 sows statistically proven patterns were not found, there was only a tendency to slight predominance of sows with ESR1BB and ESR1AB genotypes comparing to individuals with ESR1AA genotype. In the experimental group of Mirgorod sows there was a tendency to have most part of individuals with heterozygous genotype. Analysis of prolificacy of ULW-3 sows due to their genotype for the estrogen receptor 1 gene confirmed the superiority of ESR1BB and ESR1AB genotypes comparing to ESR1AA sows. According to 2nd-4th farrows, sows with ESR1BB and ESR1AB genotypes had the advantage in prolificacy comparing to ESR1AA sows by 1.15 and 0.53 piglets, respectively. According to the 1st farrowing difference between genotypes was absent. ESR1/PvuII-polymorphism do es not influence on prolificacy of ULW-1 sows. According to the 1st farrowing the trend towards a higher level of prolificacy of Mirgorod sows with ESR1AA genotype was found, while difference in 2nd-4th farrows between the groups was absent. It was found that ESR1/PvuII-polymorphism impact on the total number of piglets at birth and prolificacy for ULW-3 sows is characterized by predominance of additive component with a little contribution of the dominant component, the similar trend is observed for ULW-1 sows. There is a complex nature of the impact of ESR1/PvuII-polymorphism on the reproductive traits of Mirgorod sows in the predominance of the dominant component. Conclusions. The impact of polymorphism in estrogen receptor 1 gene on the total number of piglets in the litter after the birth and prolificacy in ULW-3 sows was detected. ULW-3 sows with ESR1BB genotype have 1.36 more piglets in a litter (analysing data from 2nd-4th farrows) and 1.15 more comparing to animals with ESR1AA genotype. ESR1/PvuII-polymorphism was not associated with total number of piglets in a litter and prolificacy in ULW-1 sows and Mirgorod sows. The counted parameters of additive-dominant model indicate that ESR1/PvuII polymorphism impact on the total number of piglets at birth and prolificacy for ULW-3 sows is characterized by predominance of additive component with a little contribution of the dominant component.

scholarly journals ЗВ'ЯЗОК G.143C>T ПОЛІМОРФІЗМУ ГЕНА CTSL ІЗ ВІДГОДІВЕЛЬНИМИ І М’ЯСНИМИ ЯКОСТЯМИ СВИНЕЙ ВЕЛИКОЇ БІЛОЇ ПОРОДИ УКРАЇНИ

2014 ◽  
pp. 88-90
Author(s):  
К. Ф. Почерняєв ◽  
В. М. Балацький ◽  
І. К. Лядський
Keyword(s):  
Impact Force ◽  
Large White ◽  
Meat Yield ◽  
Large White Breed ◽  
White Breed ◽  
Different Populations ◽  
The Impact

Викладені результати досліджень зв’язку гена ctslіз відгодівельними і м’ясними якостями свиней вели-кої білої породи України. Проаналізовано особливостіалелофонду гена ctsl у різних популяціях свиней ВБпороди; проведено аналіз частот і розповсюдженнярізних генотипів; вивчено зв'язок g.143C>T полімор-фізму цього гена з товщиною відкладення сала посередині спини та в ділянці 6–7-го ребер, а також ізсередньодобовими приростами,товщиною найдовшо-го м’яза спини й виходом м’яса. Визначено силу впли-ву g.143C>T поліморфізму гена ctsl на ці ознаки.Встановлено достовірні відмінності (P<0,05) за де-якими з вищезгаданих ознак у свиней із різними гено-типами гена ctsl. На підставі проведених дослідженьзроблені висновки та подані пропозиції виробництву. The results of researches of the association of gene ctsl   with the formation of fattening and meat qualities in pigs of the Large White breed of Ukraine are given. It has been analysed the features of gene ctsl alelofund in different populations of pigs of LW breed, it was carried out the analysis of frequencies and distribution of different genotypes. The association g.143C>T polymorphism of this gene with thick deposits of fat in the middle of the back and in area of 6-7 ribs, as well as average daily gains , thickness of the longest muscles and meat yield was studied. It has been determined the impact force g.143C>T polymorphism of gene ctsl on these traits. It was found out significant differences (P <0.05) for some of the above traits in pigs with different genotypes of gene ctsl. On the basis of the researches it was made conclusions and advanced proposals to producers.

scholarly journals Analysis of prolificacy in sows of hyperprolific lines of Large White breed

2011 ◽  
Vol 50 (No. 4) ◽  
pp. 155-162
Author(s):  
V. Matoušek ◽  
A. Čermáková ◽  
N. Kernerová ◽  
P. Králová
Keyword(s):  
Candidate Genes ◽  
Reproductive Performance ◽  
Reproductive Traits ◽  
Breeding Value ◽  
Large White ◽  
Large White Breed ◽  
Lifetime Performance ◽  
White Breed ◽  
Positive Effect ◽  
Esr Gene

The objective of the paper was to evaluate the results of reproductive performance of sows in some elite breeding herds of the Large White breed included in experimental herds for the production of hyperprolific lines of dam breeds. The set consisted of 98 sows with the known genotypes of ESR, FSH&acirc; and PRLR genes. The dendrogram shows that on the basis of their genetic outfit the sows can be divided into two clusters. The first cluster can be described as a cluster with marked dominance of HPL sows and the second cluster with marked dominance of the sows of basic herd. The first cluster consisted of individuals in which the preferred genotype AA of PRLR gene was not detected. As for FSH&acirc; gene, the beneficial genotype BB was found out in 74.10% of sows. In ESR gene the beneficial genotype DD was recorded only in 11.10% of individuals. On average for the lifetime performance they delivered by 2.08 piglets more in all born piglets and by 1.96 piglets more in live-born piglets per litter. Differences in the reproductive traits between HPL sows and the sows of basic herd in the first cluster were statistically highly significant. On the contrary, genotype AA of PRLR gene was identified in all sows of the second cluster, 61.36% of animals possessed beneficial genotype BB of FSH&acirc; gene. As for ESR gene, beneficial genotype DD was identified within the whole cluster in 31.82% of sows. In lifetime performance the HPL sows had on average by 1.10 individuals more in all born piglets and by 1.01 more in live-born piglets (statistically significantly higher values). The unambiguous expression of a positive effect of preferred genotypes of selected candidate genes failed to be confirmed by the results of statistical analyses testing the associations of candidate genes for pig reproduction with selected parameters of breeding value and prolificacy of sows. &nbsp;

scholarly journals 7. Associations between Polymorphisms in the DIO3 Gene and Reproductive Traits and Carcass Performance in Pigs

2016 ◽  
Vol 16 (2) ◽  
pp. 399-413
Author(s):  
Maria Oczkowicz ◽  
Anna Dunkowska ◽  
Katarzyna Piórkowska ◽  
Aurelia Mucha ◽  
Mirosław Tyra ◽  
...  
Keyword(s):  
Association Study ◽  
Candidate Gene ◽  
Carcass Traits ◽  
Reproductive Traits ◽  
Large White ◽  
The Third ◽  
Large White Breed ◽  
Population Average ◽  
White Breed ◽  
Carcass Performance

Abstract Recently, DIO3 gene has been proposed as a candidate gene for litter size in pigs. Moreover, it was shown that polymorphism in this gene is associated with carcass traits. In this study we identified several SNPs within coding sequence of DIO3 by HRM method and performed association study between two polymorphisms and reproductive and carcass traits in pigs bred in Poland. Analysis of 350 pigs of Landrace and Large White breed revealed several significant associations for rs80999359, like period between the second and third parities (2IP)(P<0.0008) in the whole population, period between the third and fourth parities (3IP) (P<0.022), number of piglets born alive (L3NBA) (P<0.0084) and number of piglets at 21 days (L3NB21d) (P<0.0176) at the third parity in Large White as well as period between the second and third parities (2IP) (P<0.0012) in Landrace breed. The second polymorphism (rs80983654) was associated with 1IP (P<0.0218), number of piglets born alive at the fourth parity (L4NBA, P<0.027), number of piglets at 21 day at the fourth litter (L4NB21d, P<0.01), in the whole population, average number of piglets born alive (ANBA, P<0.01250), average number of piglets at 21 day (ANB21d, P<0.009), average interparity period (AIP, P<0.016), age at the first parity (1AP, P<0.003), (1IP, P<0.001, L4NBA, P<0.017, L4NB21d, P<0.005) in Large White breed. In contrast, we have found only few associations between DIO3 polymorphisms and carcass traits. rs80999359 was associated with backfat thickness (p<0.01) while rs80983654 with the weight of ham. Our results suggest that polymorphisms within DIO3 gene may be associated with reproductive traits.

scholarly journals Polymorphism of Selected Microsatellite Markers against Breeding Performance Indexes of Polish Large White and Polish Landrace Sows

Agriculture ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 591
Author(s):  
Błażej Nowak ◽  
Anna Mucha ◽  
Magdalena Moska ◽  
Magdalena Zatoń-Dobrowolska ◽  
Wojciech Kruszyński
Keyword(s):  
Microsatellite Markers ◽  
Litter Size ◽  
Dna Isolation ◽  
Reproductive Traits ◽  
Weaned Piglets ◽  
Breeding Performance ◽  
Large White ◽  
Large White Breed ◽  
Performance Indexes ◽  
White Breed

The study aimed to analyze 12 microsatellite markers located in the areas of quantitative trait loci related to litter size in 82 sows, including 45 Polish Large White and 37 Polish Landrace sows, kept on a farm in southwest Poland. Breeding documentation provided data on the total number of piglets born per litter and the numbers of live-born, stillborn and weaned piglets; the corresponding percentage values were also calculated. DNA isolation was performed from 15–20 hairs taken from live animals, and the markers used in the research were divided into four multiplexes. The influence of genotype at a given locus on the reproductive traits was analyzed only for genotypes represented by at least five animals. The results of the analyses for all the sows (treated as the maternal component and not as distinct breeds) showed statistically significant (p ≤ 0.05) differences between the genotypes and the values of the analyzed traits for markers S0008, SW160, SW245, SW714, SW1125 and SW2411. Among these markers, the breed-dependent analysis also showed significant differences in the Polish Large White breed for markers S0008, SW160 and SW1125, and in the Polish Landrace breed for SW245. Additionally, the breed-dependent analysis found significant differences in markers SW903 and SW1808 for the Polish Large White sows, and S0064, SW472 and SW903 in Polish Landrace sows. SW903 was the only marker for which differences in the analyzed reproductive traits differed between genotypes in both breeds (still analyzed separately), although in terms of different traits. The above results indicate the usefulness of microsatellite markers in researching the differentiation of litter size indicators. Although both breeds belong to the maternal component, they showed significant differences in terms of markers. This may suggest difficulties in finding universal (that is, working well for various breeds of the maternal component) markers, indicating the need to look for breed-specific markers, something that calls for further research into numerous animals.

Association between leptin receptor gene polymorphism and quality of both meat and back fat in large white pigs of ukrainian breeding

2016 ◽  
Vol 3 (2) ◽  
pp. 42-48
Author(s):  
V. Balatsky ◽  
I. Bankovska ◽  
A. Saienko
Keyword(s):  
Gene Polymorphism ◽  
Leptin Receptor ◽  
Receptor Gene ◽  
Intramuscular Fat ◽  
Large White ◽  
Pig Breeds ◽  
Lepr Gene ◽  
Large White Breed ◽  
White Breed

Leptin receptor is one of the components of the system of regulating energy homeostasis of the organism. Leptin receptor gene (LEPR) polymorphism is associated with pig carcass index of the content of intramus- cular fat in its valuable parts, which is particularly important when assessing the quality of their carcasses for processing. Intramuscular fat is associated with meat fl avor characteristics and partly determines its tenderness, juiciness, and other parameters. Aim. To analyze LEPR gene (SNP NM001024587.1, p. 1987 C > T) polymor- phism in populations of various pig breeds and to establish its relationship with the quality of both meat and fat of pigs of Large White breed of Ukrainian breeding. Methods. Genetic-population analysis of nine pig breeds, associative analysis on the search connection of LEPR gene polymorphism with quality of both meat and fat of pigs of Large White breed of Ukrainian breeding. LEPR locus genotyping was performed by High Resolution Melting (HRM). Results. All the studied breeds are characterized by polymorphism of the leptin receptor gene (SNP NM001024587.1, p. 1987 C > T), signifi cant breed specifi city in the distribution of frequencies of alleles was established. Statistically confi rmed effect (p < 0.05) of genotypes LEPR on the content of intramuscular fat, total dry matter and moisture in the meat, as well as the moisture content in the back fat of pigs of Ukrainian Large White breed was revealed. Higher content of intramuscular fat was found in the animals with genotype TT, while a smaller amount of intramuscular fat and more moisture in fat was revealed in heterozygotes. Conclusions. Genetic marker LEPR SNP NM001024587.1, p. 1987 C > T can be used in the marker-assisted selection to predict and improve the performance quality of the meat of pigs of Large White breed of the Ukrainian breeding. These results suggest that porcine leptin receptor gene controls the quality of fat comp- lex – inside muscles and in the dorsal part of the carcass.

Indexes of reproductive ability and their repeatability in sows of different genotypes, taking into account the polymorphism g.1426g> a of the Mc4r gene

2020 ◽  
pp. 49-62 ◽  
Author(s):  
Viktor Khalak ◽  
Vasyl Voloshchuk ◽  
Konstiantyn Pochernyaev ◽  
Serhii Smyslov ◽  
Maria Ilchenko
Keyword(s):  
Receptor Gene ◽  
Live Weight ◽  
Multiple Births ◽  
Large White ◽  
Mc4r Gene ◽  
Litter Weight ◽  
Reproductive Ability ◽  
Large White Breed ◽  
White Breed ◽  
Consolidation Coefficient

The results of studies of the reproductive ability of sows of different genotypes are given taking into account the polymorphism g.1426G> A of the MC4R gene, the factor of repeatability of characters is determined, and the economic efficiency of the research results is calculated. The experimental part of the research was carried out in the conditions of the «Druzhba-Kaznacheevka» LTD in Dnipropetrovsk region. The objects of research were sows of the Large White breed. It was determined that sows of the main herd, according to signs of reproductive ability, belong to class I and class elite. The experiment showed that sows of genotype AG exceeded their peers of others (GG and AA) in terms of “born piglets total, heads” by 1.6 and 0.7 heads, “Live piglets born (multiple births), heads - by 1 6 and 0.9 heads, “the mass of the litter at the time of birth, kg” - by 2.7 - 1.3 kg., “the mass of the litter at the time of weaning at the age of 28-35 days, kg” - by 5, 1 - 3.9 kg. The difference between animals of the indicated genotypes is: for large-foetus - 0.06-0.01 kg, the index "evenness of the sow’s litter in live weight of piglets at birth, points" - 1.05-1.84 points, index of N. D. Berezovsky - 3.75 -2.36 points. The index of the safety of piglets before weaning ranged from 84.4 to 93.0 %. It was proved that the minimum value of the index “evenness of the sow’s littar in live weight of piglets at the time of birth, points (7.76 ± 1,082 points) are characterized by sows of genotype AA, in which the rate of multiple pregnancy ranged from 8 to 14 pigs, and the value of the sign“ large-foetus, kg "equaled 1.37 kg. The repeatability coefficient for sows of the main herd and sows of the Large White breed of different genotypes for the melanocortin 4 (MC4R) receptor gene ranges from -0.916 ± 0.1418 (І-IV farrow, genotype - GG, sign - “piglet safety before weaning, %) to 0.978 ± 0.0738 (I-V farrow, genotype - GG, sign - “large foetus”, kg ”). The maximum value of the phenotypic consolidation coefficient was found in sows of genotype AA according to the following quantitative characteristics: “live piglets (multiple births), heads.”, “litter weight at the time of birth, kg”, “litter weight at weaning age of 28-35 days, kg "," the safety of piglets before weaning ,% ". The sows of the genotype GG belong to the unconsolidated group according to the signs of reproductive qualities. The use of sows of the genotype for the melanocortin 4 (MC4R) AG receptor gene provides additional products at the level of 5.15 %, and its cost is 165.41 UAH /head. Keywords: sow, breed, reproductive ability, MC4R gene, genotypic consolidation coefficient, variability, correlation, repeatability.

scholarly journals Hybridization as a method of improving quality of pork

2020 ◽  
pp. 31-35
Author(s):  
B. P. Kovalenko ◽  
◽  
O. B. Shevchenko ◽  
Keyword(s):  
Chemical Composition ◽  
Physical Properties ◽  
Ph Value ◽  
Longissimus Dorsi ◽  
Large White ◽  
Group Iii ◽  
Large White Breed ◽  
White Breed ◽  
A Share

Studies were carried out on the main indicators of the chemical composition and physical properties of muskulus longissimus dorsi of pigs of different genotypes during hybridization. The indicators of the chemical composition and physical properties of muskulus longissimus dorsi were determined by conventional methods. The materials of experimental studies were processed using methods of variation statistics. According to the amount of moisture, no significant difference between the control and experimental groups was established. According to the amount of protein in meat, a tendency to a slight decrease, compared with the control group by 1.4 (group III) … 1.9 % (group II) was established. The use of boars of Poltava meat breed led to a decrease in intramuscular fat in descendants by 0.25 % compared with peers of a large white breed (P <0.95), and precocious meat - to a real increase of this indicator by 0.43 % at P> 0 , 95. A tendency towards an increase in the amount of tryptophan (by 0.04 ... 0.05) and oxyproline (by 0.004 ... 0.005) in the muscles of hybrid animals was revealed in comparison with peers of pure breed of large white breed. In terms of the biological usefulness of meat proteins, animals of group III were the best - in terms of the quality of the protein of the longest muscle of the back, their advantage over peers of group II was 0.07, and compared with purebred peers. Group I - 0.12 (p <0.95). It was found that with the same area of the meat spot both in purebred large white breeds of animals and hydrides of different genotypes, there is a tendency to increase the latter compared to the control in a humid place: with a «share» of «blood» ½ pm by 2.2 %, and with a «share» of blood ½ cm - 1.4 %. A tendency has been established to increase the pH value of the longest back muscle in animals with a «share» of blood of specialized meat breeds compared with a large white breed. In hybrid animals with a «share» of blood of Poltava meat and precocious meat breeds, there is a tendency to increase the pH value in comparison with the large white breed by 0.15 (2.8 %) and by 0.17 (3.1 %), i.e. the meat of hybrid pigs, the pH of which has a shift towards alkalinity due to less accumulation of lactic acid, has a worse preservation.

scholarly journals EFFICIENCY OF INTRAUTERINE INSEMINATION IN BREEDING SOWS

2017 ◽  
Vol 53 ◽  
pp. 254-259
Author(s):  
V. O. Melnik ◽  
O. O. Kravchenko ◽  
О. S. Kohut
Keyword(s):  
Artificial Insemination ◽  
Intrauterine Insemination ◽  
Live Weight ◽  
Reproductive Technologies ◽  
Breeding Value ◽  
Large White ◽  
Large White Breed ◽  
Significant Difference ◽  
White Breed ◽  
Breeding Farms

Improving of fertility indicators and reproductive qualities of sows during artificial insemination in farms of different specializations is very topical issue. The introduction of artificial insemination of sows on breeding farms by the spermdoses of optimum volume, by the frozen-thawed and sex sperm requires the introduction of innovative reproductive technologies. The use of economical methods of artificial insemination of sows using a minimum number of sperm in a small volume of spermdose in order to achieve high rates of fertility and prolificacy was proven in numerous experiments of the authors. Significantly reduced spermdose may be sufficient if the sperm enters deep enough into the uterus. Vitality of sperm does not depend on the size of spermdose, but the best place for sperm to survive one oviducts where they keep the fertilizing capacity from 9 to 27 hours. So deep intrauterine insemination of sows improves conditions for sperm survival The aim was to study the feasibility and justification for widespread implementation in to production on breeding farms of intrauterine insemination of sows morder to increase their fertilization and prolificacy and  to save the boar sperm with the highest index of breeding values. Experiments were conducted in terms of selection and genetic center of Agrofirm "Mig-Service-Agro" in Mykolaiv region. In the experiment used 65 sows of live weight of 280-320 kg wiht 2-4 farrowing were. Sows in sexual hunting were showed once daily in the morning using a boar-prober. Artificial insemination was performed twice: the first time - in the afternoon and at 14-16 p.m. The second time – in the morning of the next day at 9-10 am. For artificial insemination of sows were ed using experimental spermodes with volume of 40 ml which contained 1.5 billion of a Active sperm. For the dilution of sperm was used Durasperm - KRUUSE (Denmark) the period of sperm perpetuation is 5-7 days. To enter the were semen used catheters Magaplus S, (Spain) for intrauterine insemination of sows. Analysis shows that the period from weaning to insemination has significant difference comparing sows of large White breed with Landrace breed (p <0.001), with genotype sows F1 (p <0.01) and sows of the Duroc breed (p <0.05). For all selected 65 sows duration of suckling period, was estimated which averaged 32.2 days and the average time from weaning of pigs to their sexual inclination and the first intrauterine insemination 6.8 days that meets the physiological norm. After intrauterine insemination of sows of then 48 farrowed, which wich made for 73.9%. іncluding live 5 emergency farrow  were obtained, representing 10.4% of all amount. Percentage of farrow is considered physiologically normal – 80%, or more of total insemined sows. The very low percentage of farrow 53.3% had of sows F1, and the highest percentage was found by sows of the Duroc breed – 85.7%. Pregnency of sows were received just 17, which made 26.1% and highest percentage – 46.7% was set by sows F1. Analysis of the pregnancy sows shows that on average it is 116.2 days was the longest – 117.1 days was set in Landrace breed sows and the short est 115.5 days in Large White breed, but the difference is not significant. 571 pigs were received, іncluding live 451 head, which is 78.9%. The largest percentage of іncluding live piglets obtained from sows F1 – 82.5%, and the lowest in Landrace breeds – 77.0% and Large White – 77.3%. Exit of all piglets per sow without emergency farrowings is 12.2, іncluding live – 9,8. The highest yield were obtained piglets from sows F1 – 13.1, іncluding live – 10.9, the lowest yield of sows of the Duroc breed – 10.6, іncluding live 9.1, which has significant difference compared with the control (IDPs) and other breeds.  After intrauterine insemination 8 sows showed cyclic deregulation in 20-25 days, ie repeated sexual hunt took place on average 22.3 days. These sows were inseminated by not fractional way, they farrowed and an average litter just 13.1 piglets per sow, іncluding live - 11.3 was obtained. Repeating after intrauterine insemination on 45-48-49 day in the sexual hunt came about three sows for artificial insemination by not fractional method 3 farrowed and was obtained output – 13.3 piglets, іncluding live – 11.7. It should be noted that the best sow Large White breed №12 after intrauterine insemination bore 16 pigs, іncluding live 11, sow of Landrace breed №1556 – 18 pigs, іncluding live 12, Duroc №5888 – 13 pigs, іncluding live 11, sow F1 №167 – 20 pigs, іncluding live 14 pigs. Breeding requires more careful handling with major sows taking into account their breeding value and cost, that’s why we believe that there is no need to risk causing injury genitals with intrauterine insemination if a sufficient number of spermdoses of boars-sires exist.

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