Potential and Pitfalls of the DNA Metabarcoding Analyses for the Dietary Study of the Large Japanese Wood Mouse Apodemus speciosus on Seto Inland Sea Islands

Mammal Study ◽  
2019 ◽  
Vol 44 (4) ◽  
pp. 221 ◽  
Author(s):  
Jun J. Sato ◽  
Daisuke Kyogoku ◽  
Taketo Komura ◽  
Chiaki Inamori ◽  
Kouhei Maeda ◽  
...  
Keyword(s):  
Wood Mouse ◽  
Seto Inland Sea ◽  
Sea Islands ◽  
Apodemus Speciosus ◽  
Dna Metabarcoding ◽  
Dietary Study

scholarly journals DNA metabarcoding dietary analyses of the wood mouse Apodemus speciosus on Innoshima Island, Japan, and implications for primer choice

2021 ◽  
Author(s):  
Jun J. Sato ◽  
Yuya Ohtsuki ◽  
Naoki Nishiura ◽  
Kumi Mouri
Keyword(s):  
Wood Mouse ◽  
Early Spring ◽  
Stink Bug ◽  
Stink Bugs ◽  
Apodemus Speciosus ◽  
Gypsy Moths ◽  
Dna Metabarcoding ◽  
Citrus Orchards ◽  
Human Ecosystems ◽  
Chinese Cork Oak

Abstract We used DNA metabarcoding to assess the seasonal diets of the large Japanese wood mouse, Apodemus speciosus (Muridae, Rodentia), in forest edges adjacent to citrus orchards on Innoshima Island, Japan. We used one chloroplast and three mitochondrial DNA barcoding markers to determine mouse diets. Among the various plant and invertebrate diets, A. speciosus typically consumed Chinese cork oak (Quercus variabilis) in early spring (likely acorns preserved during winter) and gypsy moths (Lymantria dispar, a forest pest) in late spring and summer. In addition, we found that A. speciosus also preyed on orchard pests, including the gutta stink bug and other potentially harmful invertebrates. The season during which A. speciosus preyed on stink bugs corresponded with the harvest of orchard products. This study revealed several of the ecological roles of A. speciosus within the boundary zone between forest and human ecosystems. Furthermore, based on the performance of various mitochondrial markers in dietary profiling of invertebrate food items, we recommend the multi-locus DNA metabarcoding method to comprehensively assess the diet of A. speciosus.

Seasonal changes in the hypothalamo-pituitary-testes axis of the japanese wood mouse (Apodemus speciosus)

2000 ◽  
Vol 260 (4) ◽  
pp. 366-372 ◽  
Author(s):  
Sachi Kuwahara ◽  
Takuo Mizukami ◽  
Masako Omura ◽  
Mio Hagihara ◽  
Yasuko Iinuma ◽  
...  
Keyword(s):  
Seasonal Changes ◽  
Wood Mouse ◽  
Apodemus Speciosus

Negative effects of acorns on the wood mouse Apodemus speciosus

2003 ◽  
Vol 45 (1) ◽  
pp. 7-17 ◽  
Author(s):  
Takuya Shimada ◽  
Takashi Saitoh
Keyword(s):  
Wood Mouse ◽  
Negative Effects ◽  
Apodemus Speciosus

scholarly journals Mitochondrial Phylogeography and Population History of the Large Japanese Wood Mouse (Apodemus speciosus) on Sado Island, Japan

Mammal Study ◽  
2010 ◽  
Vol 35 (2) ◽  
pp. 93-97 ◽  
Author(s):  
Morihiko Tomozawa ◽  
Hiroshi Tomida ◽  
Hitoshi Suzuki ◽  
Kimiyuki Tsuchiya
Keyword(s):  
Wood Mouse ◽  
Population History ◽  
Apodemus Speciosus ◽  
History Of

scholarly journals Draft genome sequence of Japanese wood mouse, Apodemus speciosus

Data in Brief ◽  
2018 ◽  
Vol 16 ◽  
pp. 43-46 ◽  
Author(s):  
Masatoshi Matsunami ◽  
Daiji Endo ◽  
Naruya Saitou ◽  
Hitoshi Suzuki ◽  
Manabu Onuma
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
Wood Mouse ◽  
Draft Genome Sequence ◽  
Apodemus Speciosus

scholarly journals Lactobacillus apodemi sp. nov., a tannase-producing species isolated from wild mouse faeces

2006 ◽  
Vol 56 (7) ◽  
pp. 1693-1696 ◽  
Author(s):  
Ro Osawa ◽  
Tomohiko Fujisawa ◽  
Rüdiger Pukall
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Wild Mouse ◽  
Wood Mouse ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Apodemus Speciosus

A Gram-positive, rod-shaped, non-endospore-forming bacterium, strain ASB1T, able to degrade tannin, was isolated from faeces of the Japanese large wood mouse, Apodemus speciosus. Comparative analysis of the 16S rRNA gene sequence revealed that the strain could be assigned as a member of the genus Lactobacillus. The nearest phylogenetic neighbours were determined as Lactobacillus animalis DSM 20602T (98.9 % 16S rRNA gene sequence similarity) and Lactobacillus murinus ASF 361 (98.9 %). Subsequent polyphasic analysis, including automated ribotyping and DNA–DNA hybridization experiments, confirmed that the isolate represents a novel species, for which the name Lactobacillus apodemi sp. nov. is proposed. The DNA G+C content of the novel strain is 38.5 mol%. The cell-wall peptidoglycan is of type A4α l-lys–d-Asp. The type strain is ASB1T (=DSM 16634T=CIP 108913T).

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