DIN EN ISO 10272-2:2017-09, Mikrobiologie der Lebensmittelkette_- Horizontales Verfahren zum Nachweis und zur Zählung von Campylobacter spp._- Teil_2: Koloniezählverfahren (ISO_10272-2:2017); Deutsche Fassung EN_ISO_10272-2:2017

2017 ◽  
Keyword(s):  
Campylobacter Spp

Risk assessment of Campylobacter species in shellfish: identifying the unknown

1997 ◽  
Vol 35 (11-12) ◽  
pp. 29-34 ◽  
Author(s):  
P. Teunis ◽  
A. Havelaar ◽  
J. Vliegenthart ◽  
G. Roessink
Keyword(s):  
Risk Assessment ◽  
Quantitative Risk Assessment ◽  
Contamination Level ◽  
Risk Of Infection ◽  
Worst Case ◽  
Considerable Uncertainty ◽  
Risk Estimates ◽  
Consumption Data ◽  
Campylobacter Infection ◽  
Campylobacter Spp

Shellfish are frequently contaminated by Campylobacter spp, presumably originating from faeces from gulls feeding in the growing or relaying waters. The possible health effects of eating contaminated shellfish were estimated by quantitative risk assessment. A paucity of data was encountered necessitating many assumptions to complete the risk estimate. The level of Campylobacter spp in shellfish meat was calculated on the basis of a five-tube, single dilution MPN and was strongly season-dependent. The contamination level of mussels (<1/g) appeared to be higher than in oysters. The usual steaming process of mussels was found to completely inactivate Campylobacter spp so that risks are restricted to raw/undercooked shellfish. Consumption data were estimated on the basis of the usual size of a portion of raw shellfish and the weight of meat/individual animal. Using these data, season-dependent dose-distributions could be estimated. The dominant species in Dutch shellfish is C. lari but little is known on its infectivity for man. As a worst case assumption, it was assumed that the infectivity was similar to C. jejuni. A published dose-response model for Campylobacter-infection of volunteers is available but with considerable uncertainty in the low dose region. Using Monte Carlo simulation, risk estimates were constructed. The consumption of a single portion of raw shellfish resulted in a risk of infection of 5–20% for mussels (depending on season; 95% CI 0.01–60%). Repeated (e.g. monthly) exposures throughout a year resulted in an infection risk of 60% (95% CI 7–99%). Risks for oysters were slightly lower than for mussels. It can be concluded that, under the assumptions made, the risk of infection with Campylobacter spp by eating of raw shellfish is substantial. Quantitative risk estimates are highly demanding for the availability and quality of experimental data, and many research needs were identified.

Prevalence of thermophilic Campylobacter spp. in non-human primates of the Macaca mulatta species from a breeding center for use as biomodels

2018 ◽  
Vol 50 (1) ◽  
Author(s):  
Paula Borba Cruz ◽  
Fernanda Nunes Santos ◽  
Wagner Thadeu Cardoso Esteves ◽  
Jaime Antonio Abrantes ◽  
Fabio Alves da Silva ◽  
...  
Keyword(s):  
Macaca Mulatta ◽  
Thermophilic Campylobacter ◽  
Breeding Center ◽  
Campylobacter Spp

scholarly journals INDAGINE CONOSCITIVA INTERREGIONALE SULLA RICERCA COLTURALE DI CAMPYLOBACTER SPP.

2004 ◽  
Vol 19 (2) ◽  
Author(s):  
F. Bernieri ◽  
L. Merlino ◽  
M. Agnello ◽  
A. Buratta ◽  
M. Borsotti ◽  
...  
Keyword(s):  
Campylobacter Spp

Spiral-shaped bacteremia: Difference in the duration of blood cultures between Helicobacter spp. and Campylobacter spp. using the BACTEC system

2021 ◽  
Vol 182 ◽  
pp. 106158
Author(s):  
Kazuhiro Itoh ◽  
Hiromichi Iwasaki ◽  
Hiroko Shigemi ◽  
Hiroshi Tsutani ◽  
Takahiro Yamauchi
Keyword(s):  
Blood Cultures ◽  
Bactec System ◽  
Campylobacter Spp

scholarly journals Evaluation of CAMPYLOBACTER QUIK CHEK™ rapid membrane enzyme immunoassay to detect Campylobacter spp. antigen in stool samples

Gut Pathogens ◽  
2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Justine Franco ◽  
Lucie Bénejat ◽  
Astrid Ducournau ◽  
Francis Mégraud ◽  
Philippe Lehours ◽  
...  
Keyword(s):  
Screening Method ◽  
Positive Culture ◽  
National Reference ◽  
Negative Culture ◽  
Membrane Enzyme ◽  
Adults And Children ◽  
Stool Samples ◽  
National Reference Center ◽  
Campylobacter Spp ◽  
Composite Reference Standard

AbstractCampylobacter spp. enteritis is the most frequent bacterial enteritis in both adults and children and is sometimes a source of severe complications. Its diagnosis by culture suffers from a lack of sensitivity and delays the result, preventing an early initiation of optimal antibiotic therapy in some cases. Our aim was to test a new rapid immuno-enzymatic method for Campylobacter spp. diagnosis in comparison to a composite reference standard (CRS). Stool samples from the French National Reference Center for Campylobacter and Helicobacter were tested with the CAMPYLOBACTER QUIK CHEK™ (Abbott). The CRS used to consider a case positive for Campylobacter spp. was a positive culture and, in case of a negative culture, a positive result obtained with both an ELISA and a molecular test. One hundred and eight stools were included: 53 were positive according to the CRS. If performed alone, culture would have missed 5 cases which the CAMPYLOBACTER QUIK CHEK™ detected. Finally, the CAMPYLOBACTER QUIK CHEK™ showed a sensitivity of 96.2% and a specificity of 94.5% and is relevant for clinical practice. Given the characteristics of the new method, it can be used as a screening method for Campylobacter spp. detection.

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