scholarly journals GC-MS analysis of yellow pigmented Macrococcus equipercicus isolated from alfalfa rhizosphere soil fields of Coimbatore

2019 ◽  
Vol 11 (3) ◽  
pp. 645-649
Author(s):  
Z. Aiysha Thasneem ◽  
K. Aravindh ◽  
M. Jeba Malar Fencia ◽  
C. Nitheesh Kumar ◽  
T. Pavithra ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Rhizosphere Soil ◽  
Chemical Compounds ◽  
Bioactive Components ◽  
Streptococcus Pneumonia ◽  
Ms Analysis ◽  
Rrna Sequencing ◽  
Gram Positive Cocci ◽  
Acid Esters

The rhizosphere of plant possesses important microflora, which secretes wide chemical compounds including secondary metabolites necessary for plant growth and development. The microbial flora of alfalfa plant rhizosphere soil region was explored for functional activity and we found upto ten different pigmented colonies. Due to good functional diversity, this yellow pigmented colony was taken for further studies. Thus, the culture was molecularly characterized and identified for potent bioactive components responsible for antimicrobial activity. The selected culture mass was cultured and secondary metabolites were produced and extracted using ethyl acetate and subjected to GC-MS analysis. The antimicrobial study revealed selective activity against Streptococcus pneumonia, and Proteus sp with zone of inhibition to be 18 and 20 mm respectively.  Molecular identification of the isolate by 16S rRNA sequencing showed the isolate as Macrococcus equipercicus with 100 % similarity. Based on GC-MS analysis report 25bioactive compounds were identified and 13-docosenamide, hexadecanoic acid esters and quercetin were found in ethyl acetate extract. Conclusion: Thus the yellow pigmented gram positive cocci M.equipercicus isolated from Medicago sativa possessed wide antibacterial activity due to presence of quercetin. Through the studies, we were able to identify potent antibacterial compound producing bacteria from M. sativa plant rhizosphere soil.

scholarly journals Isolasi Senyawa Kimia Stigmastan-3,5-Diena Yang Mempunyai Daya Toksik Dari Daun Ekaliptus (Eucalyptus Deglupta Blume.)

2017 ◽  
Vol 15 (1) ◽  
pp. 1
Author(s):  
Sari Setianingsih ◽  
Rudi Kartika ◽  
Partomuan Simanjuntak
Keyword(s):  
Ethyl Acetate ◽  
Toxicity Test ◽  
Ethyl Acetate Extract ◽  
Chemical Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Test Results ◽  
Phytochemical Screening ◽  
Isolation And Identification ◽  
Lc50 Value ◽  
Ms Analysis

This study was started by extraction of Eucalyptus deglupta Blume. Using organic solvent   (n-hexane, ethyl acetate, ethanol and water) followed by phytochemical screening and toxicity test using Brine Shrimp Lethality Test (BSLT) method. Isolation and identification of chemical compounds contained in the fraction were done by column chromatography and Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Phytochemical screening revealed the presence of alkaloids, flavonoids, steroids and phenolics in the extract. Toxicity test results showed that the ethyl acetate extract was potentially active with LC50 value of  617.95 ppm. The extract was continued to isolation stage and gave fraction EKEA-3.1 with LC50 value of 2759.93 ppm. Identification of chemical compounds in EKEA-3.1 with KG-MS analysis showed that EKEA-3.1 was suspected to be Stigmastan-3,5-diene.

scholarly journals Antibacterial Activity and Chemical Composition of Turkish Propolis

2001 ◽  
Vol 56 (11-12) ◽  
pp. 1112-1115 ◽  
Author(s):  
Nevin Keskin ◽  
Selçuk Hazir ◽  
K. Hüsnü Can Baser ◽  
Mine Kürkçüoglu
Keyword(s):  
Antibacterial Activity ◽  
Chemical Compounds ◽  
Antibacterial Activities ◽  
Fatty Acid Esters ◽  
E Coli ◽  
Weak Activity ◽  
Gram Positive Cocci ◽  
Acid Esters

Abstract The antibacterial activities of propolis samples have been examined in vitro, according to the principles accepted for the determination of a similar activity of antibiotics with the use of solid and liquid media. It has been found that propolis extracts showed antibacterial activity through a range of commonly encountered gram positive cocci (5. aureus, beta hem. Streptococus), but had weak activity against gram negative bacteria (E . coli, P. aeruginosa). GC/MS analysis showed that propolis samples contain a variety of chemical compounds in­ cluding aromatic compounds, fatty acid esters and sesquiterpenes.

scholarly journals SCREENING OF BIOACTIVE COMPOUNDS FROM UNKNOWN MARINE SPONGES COLLECTED FROM KOVALAM, CHENNAI

2017 ◽  
Vol 10 (5) ◽  
pp. 231 ◽  
Author(s):  
Yuvarani Thambidurai ◽  
Skm Habeeb ◽  
Joe Kizhakudan ◽  
Sudarsanam D
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Secondary Metabolites ◽  
Bioactive Compounds ◽  
Chemical Compounds ◽  
Marine Sponges ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Dysidea Herbacea

Objective: This study is designed to hunt for the presence of bioactive compounds from three marine sponges collected from Kovalam.Methods: Zoochemical analysis is performed to screen for the presence of secondary metabolites. Based on those results, only two sponges which showed a significant presence of secondary metabolites has been subjected to gas chromatography-mass spectrometry (GC-MS) analysis to identify the unknown chemical compounds present in those sponges.Results: On analyzing the results, two sponges, namely, Dysidea herbacea and Sigmadocia pumila, has shown a significant presence of secondary metabolites while the third sponge Acanthella elongata have shown moderate presence of secondary metabolites. Since the first two sponges results are remarkable, these two samples have been subjected to GC-MS analysis to separate and identify the unknown chemical compounds present in the sample.Conclusion: Samples, namely, D. herbacea and S. pumila, indicated the presence of several components. From both the sponges, eleven different secondary metabolites were identified by GC-MS. Most of these compounds are widely used in cosmetic, pharmaceutical, and other industries and therefore a vital source for industrial biotechnology and related products in healthcare and skincare.Keywords: Dysidea herbacea, Sigmadocia pumila, Acanthella elongata, Secondary metabolites, Gas chromatography-mass spectrometry analysis, Bioactive compounds.

Study effect of crude extracts of Hibiscus sabdarifa L. on some Enterobacteriaceae

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Sabreen A Kamal ◽  
Ishraq A Salih ◽  
Hawraa Jawad Kadhim ◽  
Zainab A Tolaifeh
Keyword(s):  
Escherichia Coli ◽  
Ethyl Acetate ◽  
Bacterial Growth ◽  
Chemical Compounds ◽  
Inhibition Zone ◽  
Crude Extracts ◽  
And Control

Red rose or roselle (beauty rose ) is natively known as red tea belong to Malvaceae, it is flowers use traditionally for antihypertensive hepato protective, anticancer,antidiabetic,antibacterial, cytotoxicity and antidiarreal, By preparing red tea from it's flower. In this study, we extract chemical compounds by using two solvent which are Ethanol, Ethyl acetate. so we can extract Anthocyanin which is responsible for red colour of flower with many chemical compounds. then study the effect of these extracts on 5 genera from Enterobacteriacaea which can cause diarrheae (Shigella, Salmonella, Escherichia coli, Proteus and Klebsiella ) by preparing 3 concentrations for each solvent (250, 500, 750 ) mg/ml, and control then compare with two antibiotic (Azereonam 30 mg/ml and Bacitracin 10 mg/ml ) these extracts revealed obvious inhibition zone in bacterial growth.

In-vitro bactericidal activity of a novel plant source Plumeria pudica against some human and fish pathogenic bacteria

2020 ◽  
Vol 17 ◽  
Author(s):  
Shubhaisi Das ◽  
Sunanda Burman ◽  
Goutam Chandra
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Positive Control ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Ft Ir ◽  
Aldehyde Groups

Background: The only remedy for up surging problem of antibiotic resistance is the discovery of antibacterial agents of natural origin. Objective: The present study was aimed at finding antibacterial potential of crude and solvent extracts of mature leaves of Plumeria pudica. Methods: Antibacterial activity of three different solvent extracts were evaluated in four human and four fish pathogenic bacteria by measuring the zone of inhibition and determining Minimum Inhibitory Concentration and Minimum Bactericidal Concentration values. Standard antibiotics were used as positive control. Preliminary phytochemical screening of most effective extract i.e., ethyl acetate extract, Fourier Transform Infra Red analysis and GC-MS analysis of the Thin Layer Chromatographic (TLC) fraction of ethyl acetate extract were done meticulously. All experiments were done thrice and analyzed statistically. Results: Crude leaf extracts and solvent extracts caused good inhibition of bacterial growth in all selected bacteria. Ethyl acetate extract showed highest inhibition zones in all tested strains with maximum inhibition (19.50±0.29 mm) in Escherichia coli (MTCC 739). MBC/MIC of the extracts indicated that all three solvent extracts were bactericidal. Preliminary phytochemical tests revealed the presence of tannins, steroids and alkaloids and FT-IR analysis revealed presence of many functional groups namely alcoholic, amide, amine salt and aldehyde groups. From the GC-MS analysis of TLC fraction of ethyl acetate extract five different bioactive compounds e.g., 2,4-ditert –butylphenyl 5-hydroxypentanoate, Oxalic acid; allyl nonyl ester, 7,9-Ditert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione, Dibutyl phthalate and 2,3,5,8-tetramethyl-decane were identified. Conclusion: Leaf extracts of P. pudica contain bioactive compounds that can be used as broad spectrum bactericidal agent.

scholarly journals Antibiotic-Induced Dysbiosis of Microbiota Promotes Chicken Lipogenesis by Altering Metabolomics in the Cecum

Metabolites ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 487
Author(s):  
Tao Zhang ◽  
Hao Ding ◽  
Lan Chen ◽  
Yueyue Lin ◽  
Yongshuang Gong ◽  
...  
Keyword(s):  
16S Rrna ◽  
Gut Microbiota ◽  
High Performance ◽  
Fat Deposition ◽  
16S Rrna Sequencing ◽  
Oral Antibiotics ◽  
Ms Analysis ◽  
Rrna Sequencing ◽  
Cecal Microbiota ◽  
Metabolite Network

Elucidation of the mechanism of lipogenesis and fat deposition is essential for controlling excessive fat deposition in chicken. Studies have shown that gut microbiota plays an important role in regulating host lipogenesis and lipid metabolism. However, the function of gut microbiota in the lipogenesis of chicken and their relevant mechanisms are poorly understood. In the present study, the gut microbiota of chicken was depleted by oral antibiotics. Changes in cecal microbiota and metabolomics were detected by 16S rRNA sequencing and ultra-high performance liquid chromatography coupled with MS/MS (UHPLC–MS/MS) analysis. The correlation between antibiotic-induced dysbiosis of gut microbiota and metabolites and lipogenesis were analysed. We found that oral antibiotics significantly promoted the lipogenesis of chicken. 16S rRNA sequencing indicated that oral antibiotics significantly reduced the diversity and richness and caused dysbiosis of gut microbiota. Specifically, the abundance of Proteobacteria was increased considerably while the abundances of Bacteroidetes and Firmicutes were significantly decreased. At the genus level, the abundances of genera Escherichia-Shigella and Klebsiella were significantly increased while the abundances of 12 genera were significantly decreased, including Bacteroides. UHPLC-MS/MS analysis showed that antibiotic-induced dysbiosis of gut microbiota significantly altered cecal metabolomics and caused declines in abundance of 799 metabolites and increases in abundance of 945 metabolites. Microbiota-metabolite network revealed significant correlations between 4 differential phyla and 244 differential metabolites as well as 15 differential genera and 304 differential metabolites. Three metabolites of l-glutamic acid, pantothenate acid and N-acetyl-l-aspartic acid were identified as potential metabolites that link gut microbiota and lipogenesis in chicken. In conclusion, our results showed that antibiotic-induced dysbiosis of gut microbiota promotes lipogenesis of chicken by altering relevant metabolomics. The efforts in this study laid a basis for further study of the mechanisms that gut microbiota regulates lipogenesis and fat deposition of chicken.

scholarly journals Gentiana asclepiadea L. from Two High Mountainous Habitats: Inter- and Intrapopulation Variability Based on Species’ Phytochemistry

Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 140
Author(s):  
Zorica Popović ◽  
Dijana Krstić-Milošević ◽  
Milena Marković ◽  
Vera Vidaković ◽  
Srđan Bojović
Keyword(s):  
Secondary Metabolites ◽  
Natural Populations ◽  
Chemical Compounds ◽  
Clear Separation ◽  
Plant Parts ◽  
Intrapopulation Variability ◽  
Population Origin

Natural populations of Gentiana asclepiadea L., located at two mountainous sites, were HPLC-analyzed regarding the contents of six representative secondary metabolites. The contents of swertiamarin (SWM), gentiopicrin (GP), sweroside (SWZ), mangiferin (MGF), isoorientin (ISOOR), and isovitexin (ISOV) were determined in six populations (three per study site), and separately for aboveground and belowground plant parts. PCA showed a clear separation of four groups according to the contents of the analyzed secondary metabolites. Out of six analyzed compounds, five were present in all samples and only one (SWZ) was found in Golija populations (belowground parts) but not in Vlasina populations, and its presence can be indicative of the geolocation of populations. Clear separation of groups was mostly affected by the different contents of chemical compounds in plant parts (aboveground versus belowground) and by the differences related to population origin (higher content of SWM and GP in belowground parts of individuals from Vlasina populations and higher content of MGF and ISOOR of individuals from Golija populations). The results of this study contribute to the spatiochemical profiling of G. asclepiadea populations and a better understanding of inter- and intrapopulation variability of pharmacologically important compounds.

Determination of Bioactive Components of 60°С Volatiles from Cinnamomum camphora Branches by TD-GC/MS

2011 ◽  
Vol 230-232 ◽  
pp. 852-856
Author(s):  
Qing Li ◽  
Dang Quan Zhang ◽  
Qi Mei Liu ◽  
Kuan Peng
Keyword(s):  
Chemical Compounds ◽  
Chemical Components ◽  
Cinnamomum Camphora ◽  
Bioactive Components ◽  
Industrial Materials ◽  
Analytical Result ◽  
Main Components

The chemical components of helium volatiles from the fresh branches of Cinnamomum camphora were studied by TD-GC/MS. The analytical result by 60°С-based TD-GC/MS showed that 55 peaks were obtained from the helium volatiles from the fresh branches of Cinnamomum camphora and 53 chemical compounds were identified. The results showed that the main components were as: Bicyclo[2.2.1]heptan-2-one, 1,7,7-trimethyl-, (1R)- (15.4328%), 1,3-Benzodioxole, 5-(2-propenyl)- (14.881%), Tricyclo[2.2.1.0(2,6)]heptane, 1,7-dimethyl-7-(4-methyl-3-pentenyl)-, (-)- (12.694%), p-menth-1-en-8-ol (9.832%), Bicyclo[2.2.1]heptane, 2-methyl-3-methylene-2-(4-methyl-3- pentenyl)-, (1S-exo)- (6.143%), 1,6,10-Dodecatrien-3-ol, 3,7,11-trimethyl- (5.365%), Bicyclo[3.1.1] hept-2-ene, 2,6-dimethyl-6-(4-methyl-3-pentenyl)- (4.527%), Naphthalene, 1,2,3,5,6,8a- hexahydro-4,7-dimethyl-1-(1-methylethyl)-, (1S-cis)- (4.129%), 3-Cyclohexen-1-ol, 4-methyl-1- (1-methylethyl)- (2.965%), Borneol (2.627%), Bicyclo[2.2.1]heptan-2-ol, 1,7,7-trimethyl-, acetate, (1S-endo)- (2.586%), Copaene (2.534%), 1,6,10-Dodecatriene, 7,11-dimethyl-3-methylene-, (Z)- (1.612%), (-)-Isosativene (1.121%), etc. The analytical result suggested that the helium volatiles from the fresh branches of Cinnamomum camphora could be used as industrial materials of biomedicines and spicery.

Vasodilatory effects and underlying mechanisms of the ethyl acetate extracts from Gastrodia elata

2017 ◽  
Vol 95 (5) ◽  
pp. 564-571 ◽  
Author(s):  
Rong Dai ◽  
Ting Wang ◽  
Xiaoqin Si ◽  
Yuanyuan Jia ◽  
Lili Wang ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Vascular Tone ◽  
Ethyl Acetate Extract ◽  
Chemical Compounds ◽  
Rat Aorta ◽  
Gastrodia Elata ◽  
Vasodilatory Effect ◽  
Intracellular Ca ◽  
Underlying Mechanisms ◽  
Ethyl Acetate Extracts

The objective of this study was to assess the ethyl acetate extracts of Gastrodia elata Blume (GEB) on vascular tone and the mechanisms involved. GEB was extracted with 95% EtOH followed by a further extraction with ethyl acetate. The effects of GEB and its ingredients on the isometric tensions of the aortic rings from rats were measured. The ethyl acetate extract of GEB induced a vasodilatory effect on rat aorta, which was partially dependent on endothelium. Four chemical compounds isolated from GEB were identified as 3,4-dihydroxybenzaldehyde (DB), 4-hydroxybenzaldehyde (HB), 4-methoxybenzyl alcohol (MA), and 4,4′-dihydroxydiphenyl methane (DM), respectively. All of these compounds induced vasodilatations, which were dependent on the endothelium to different degrees. After pretreatment with Nω-nitro-l-arginine methyl ester, indomethacin, or methylene blue, the vasodilatations induced by DB, HB, and MA were significantly decreased. In addition, the contractions of the rat aortic rings due to Ca2+ influx and intracellular Ca2+ release were also inhibited by DM. Furthermore, the administration of DB significantly enhanced the productions of nitric oxide (NO) and the activities of the endothelial NO synthase in aorta and in endothelial cells. Thus, GEB may play an important role in the amelioration of hypertension by modulating vascular tones.

scholarly journals Larvicidal Property of the Extract and Fractions of Hannoa klaineana against the Larvae of Aedes aegypti

2020 ◽  
pp. 127-132
Author(s):  
Ifeoma Chioma Ibe ◽  
Eze Elijah Ajaegbu ◽  
Lame Younoussa ◽  
Simon Pierre Yinyang Danga ◽  
Christopher Obidike Ezugwu
Keyword(s):  
Secondary Metabolites ◽  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Control Programme ◽  
Chloroform Fraction ◽  
Plant Metabolites ◽  
Lc50 Value ◽  
Synthetic Materials ◽  
Control Programmes ◽  
Crude Methanol Extract

Aedes aegypti (Culicidae) is the main vector for dengue which is endemic in Nigeria and Africa at large. Use of synthetic materials for the control of this vector has always proven abortive. The introduction of plant metabolites which is biodegradable maybe successful and safer to the human health in the control programme of this vector since plants are very rich in bioactive secondary metabolites. Hence, we evaluated the larvicidal activity of the crude methanol extract, n-hexane, chloroform, ethyl acetate and methanol fractions of Hannoa klaineana (Simaroubaceae) against the IV instar larvae of A. aegypti mosquitousing standard protocols. The most active fractions were  n-hexane, chloroform and ethyl acetate fractions with LC50 values of 1.006, 1.479, and 3.198 mg/ml respectively; n-hexane fraction having the highest property with LC50 value of 1.006 mg/ml and followed by chloroform fraction with LC50 value of 1.479 mg/ml. The evaluation has shown that the secondary metabolites of H. klaineana can be used as an alternative for the synthetic larvicides which its use has proven abortive in the vector control programmes.

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