scholarly journals DETEKSI PENYAKIT MOTILE AEROMONAS SEPTICEMIA PADA IKAN PATIN SIAM (PANGASIUS HYPOPHTHALMUS) MENGGUNAKAN METODE ELISA

2018 ◽  
Vol 1 (2) ◽  
pp. 40
Author(s):  
Herlina Maulina
Keyword(s):  
Optical Density ◽  
Indirect Elisa ◽  
Test Sample ◽  
Elisa Method ◽  
Pellet Group ◽  
A Value ◽  
Group A ◽  
Fish Collection ◽  
Group B

This study was conducted to determine the prevalence of Motile Aeromonas Septicemia of Siamese catfish farming at Cibeureum Subdistrict, Ciseeng Subdistrict, Kemang Subdistrict, and Parung Subdistrict (in Bogor Region) using antigen supernatant with concentration of RAC 1:200 and antigen pellet with concentration of RAC 1:5,000. Samples of serum obtained from 10 Siamese catfish per district have weight 300-400 g/fish. The collection of serum as much 1-4 mL obtained from 3-5 mL of blood per fish. Collection of serum which had been collected from each district then was tested using indirect ELISA method. The indirect ELISA method use the two types of dilution of serum RAC (Rabbit Anti-Catfish) and two types of antigens, i.e dilution of RAC 1:200 with antigen supernatant (group A) and dilution of RAC 1:5000 with antigen pellet (group B). Determination of the sample area exposed to positive or negative MAS disease was known of cut-off values that has been defined as a comparison, the results of the test sample with a value of OD (Optical Density) ≤ 0.011; 0,015; 0.006; 0.004; 0.000; 0.005 showed positive of MAS attacked and value of OD ≥ 0.017; 0.025; 0.018; 0.016; 0.016; 0.014 showed negative of MAS attacked. The results of research showed the prevalence 60-100% on RAC 1:5,000 dilution with antigen pellet, and the prevalence 20-60 % on RAC 1:200 dilution with antigen supernatant

scholarly journals Hematological Features in Sheep with IgG and IgM Antibodies against Borrelia burgdorferi sensu lato

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 164
Author(s):  
Labrini V. Athanasiou ◽  
Victoria M. Spanou ◽  
Eleni G. Katsogiannou ◽  
Panagiotis D. Katsoulos
Keyword(s):  
Borrelia Burgdorferi ◽  
Rural Areas ◽  
Blood Analysis ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Igm Antibodies ◽  
Group A ◽  
Group B ◽  
Group D ◽  
Immunofluorescence Antibody

Exposure of sheep to Borreliaburgdorferi sensulato (s.I.) complex, the causative agent of Lyme borreliosis (LB), has been reported in tick-abundant areas worldwide, while no data have been reported in Greece. The aim of the study was to identify the hematological alterations in sheep with seropositivity against Borrelia burgdorferi (s.I.). Blood samples were obtained from 318 tick infested sheep for blood analysis and serological determination of IgG and IgM antibodies against B. burgdorferi by indirect immunofluorescence antibody (IFA) assay after exclusion of endo-ectoparasites and other tick-borne infections. A total number of 162 sheep met the inclusion criteria, allocated in four groups based on the presence or absence of IgG and/or IgM; sheep found negative for IgM and IgG (Group A), positive for IgM (Group B), positive for both IgM and IgG (Group C) and positive for IgG (Group D). Anemia, thrombocytopenia and normal or decreased leukocyte count, mainly due to lymphopenia were the main hematological features observed in seropositive sheep. The presence of these features raises the suspicion of Borrelia infection in tick infested sheep. The seropositivity of 23.58% in sheep raises concerns of Borrelia circulation, especially in rural areas and potential risk of transmission to humans.

Effects of Mechanical Stimulation on MMP-2 and TIMP-2 Expression of Scleral Fibroblasts after Posterior Sclera Reinforcement

2014 ◽  
Vol 490-491 ◽  
pp. 867-871
Author(s):  
Guo Hui Wang ◽  
Wei Yi Chen
Keyword(s):  
Mechanical Stimulation ◽  
Sine Wave ◽  
Posterior Pole ◽  
Cyclic Stretch ◽  
Elisa Method ◽  
Band Group ◽  
Group A ◽  
Operation Group ◽  
Group B

To understand the effect of mechanical stimulation on posterior sclera reinforcement (PSR), the rabbit scleral fibroblasts after PSR were subjected to stretch in vitro and MMP-2 and TIMP-2 expression of scleral fibroblasts were evaluated. Three-week-old rabbits were monocularly performed by eyelid suturation randomly to prepare experimental myopia eye. After 60 days, the experimental myopia eyes were treated by PSR. After 6 months, the posterior pole scleral fibroblasts (normal sclera - group A, sclera after operation - group B and fusion region of sclera and reinforcing band group C) were isolated and cultured in vitro. The cells were subjected to cyclic stretch regimens (sine wave, 3% and 6% elongation amplitude, 0.1Hz, 48h duration) by FX-4000 Tension System. The MMP-2 and TIMP-2 expression of scleral fibroblasts were evaluated by ELISA method. The results show that after cyclic stretch to the scleral fibroblasts of the normal sclera and the sclera after operation, the MMP-2 expression was significantly reduced and the TIMP-2 expression was significantly increased, the MMP-2 and TIMP-2 expression of the scleral fibroblasts of the fusion region after operation was no changed. It was indicated that the mechanical stimulation could regulate the MMP-2 and TIMP-2 expression of scleral fibroblasts and play an important role in the process of treating high myopia with PSR surgery.

scholarly journals Clinical Efficacy Of Ceftriaxone Versus Ciprofloxacin In Paediatric Enteric Fever

2018 ◽  
Vol 08 (02) ◽  
pp. 67-71
Author(s):  
Anila Farhat ◽  
Asma Shaukat ◽  
Tariq Mahmood Khan
Keyword(s):  
Enteric Fever ◽  
Research Study ◽  
Controlled Trial ◽  
Clinical Effectiveness ◽  
Randomized Controlled ◽  
Group A ◽  
The Mean ◽  
Group B ◽  
Objective Determination

Objective: Determination of the clinical effectiveness of ciprofloxacin versus ceftriaxone in children with enteric fever on the basis of defervescence within 72 hours of commencement of treatment. Duration and Place of Study: This randomized controlled trial was carried out from 15th May to 15th November 2017 in pediatrics units of Benazir Bhutto Shaheed Teaching Hospital Abbottabad and Jinnah International Hospital Abbottabad. Methodology: 90 children with uncomplicated enteric fever were admitted and divided randomly into two groups, Group A was administered I/V Ceftriaxone 75mg/kg OD and Group B was given I/V Ciprofloxacin 10mg/kg BD for seven days. Response to drug was taken as defervescence within 72 hours while continued fever after 72 hours was taken as no response. The data was analyzed by using SPSS Version 21.00. Results: In our research study 53(58.9%) children were male and 37(41.1%) patients were female. The mean age was 8.43±3.17 years encompassing 4 to 14 years, mean weight of the patients in kg was 29.54±10.8 kg. Efficacy of ceftriaxone group was 93.3% while in ciprofloxacin group 62.2% patients became afebrile in 72 hours. The proportion of achieving defervescence within 72 hours was higher with ceftriaxone than with ciprofloxacin Conclusion: Ceftriaxone is more efficacious in terms of achieving defervescence than ciprofloxacin in children having enteric fever.

scholarly journals The Role of Pathological Method and Clearance Definition for the Evaluation of Margin Status after Pancreatoduodenectomy for Periampullary Cancer. Results of a Multicenter Prospective Randomized Trial

Cancers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 2097
Author(s):  
Gennaro Nappo ◽  
Domenico Borzomati ◽  
Alessandro Zerbi ◽  
Paola Spaggiari ◽  
Ugo Boggi ◽  
...  
Keyword(s):  
Prognostic Factor ◽  
Randomized Study ◽  
Periampullary Cancer ◽  
Significant Prognostic Factor ◽  
Prognostic Role ◽  
R1 Resection ◽  
Group A ◽  
Group B

Background: There is extreme heterogeneity in the available literature on the determination of R1 resection rate after pancreatoduodenectomy (PD); consequently, its prognostic role is still debated. The aims of this multicenter randomized study were to evaluate the effect of sampling and clearance definition in determining R1 rate after PD for periampullary cancer and to assess the prognostic role of R1 resection. Methods: PD specimens were randomized to Leeds Pathology Protocol (LEEPP) (group A) or the conventional method adopted before the study (group B). R1 rate was determined by adopting 0- and 1-mm clearance; the association between R1, local recurrence (LR) and overall survival (OS) was also evaluated. Results. One-hundred-sixty-eight PD specimens were included. With 0 mm clearance, R1 rate was 26.2% and 20.2% for groups A and B, respectively; with 1 mm, R1 rate was 60.7% and 57.1%, respectively (p > 0.05). Only in group A was R1 found to be a significant prognostic factor: at 0 mm, median OS was 36 and 20 months for R0 and R1, respectively, while at 1 mm, median OS was not reached and 30 months. At multivariate analysis, R1 resection was found to be a significant prognostic factor independent of clearance definition only in the case of the adoption of LEEPP. Conclusions. The 1 mm clearance is the most effective factor in determining the R1 rate after PD. However, the pathological method is crucial to accurately evaluate its prognostic role: only R1 resections obtained with the adoption of LEEPP seem to significantly affect prognosis.

Effects of chronic long-acting bromocriptine treatment on liveweight, voluntary food intake, coat growth and breeding season in non-pregnant red deer hinds

1988 ◽  
Vol 119 (3) ◽  
pp. 413-420 ◽  
Author(s):  
J. D. Curlewis ◽  
A. S. I. Loudon ◽  
J. A. Milne ◽  
A. S. McNeilly
Keyword(s):  
Food Intake ◽  
Breeding Season ◽  
Red Deer ◽  
Vehicle Group ◽  
High Dose ◽  
Group A ◽  
Long Acting ◽  
Group B ◽  
Voluntary Food Intake

ABSTRACT Seventeen red deer hinds were housed in individual pens and from 28 February until 11 November were injected each week with vehicle (group A; n = 6) or 5 (group B; n = 6) or 12·5 mg (group C; n = 5) of a long-acting formulation of bromocriptine. Liveweight and voluntary food intake (VFI) were recorded for each hind, and blood was collected for determination of progesterone, prolactin, tri-iodothyronine (T3) and cortisol concentrations by radioimmunoassay. Treatment with the high dose of bromocriptine was associated with a significant (P <0·05) reduction in VFI, with the effect being greatest between March and July. There was no treatment effect on liveweight, but there was a significant (P <0·01) interaction between time and treatment due to the faster rate of weight gain in control animals at the beginning of the experiment. Changes in liveweight could be explained by changes in VFI rather than by changes in the efficiency of utilization of intake. Termination of the breeding season was significantly (P <0·01) delayed by 54 days in group C hinds. Growth of the summer coat and subsequent winter coats was delayed by 1 and 3 months respectively in group C hinds, and in groups B and C the duration that animals were in summer coat was increased by about 1 month. The seasonal increase in prolactin concentrations was seen in all groups, but levels were significantly (P <0·05) lower in group C hinds. Concentrations of T3 and cortisol were not affected by bromocriptine. J. Endocr. (1988) 119, 413–420

scholarly journals Improving of treatment of periodontal diseases in workers of the woodworking industry of the Chernivtsi region

2021 ◽  
Vol 106 (2) ◽  
pp. 28-35
Author(s):  
V. Batig ◽  
◽  
O. Tokar ◽  
I. Burdenyuk ◽  
◽  
...  
Keyword(s):  
Maintenance Therapy ◽  
Comparison Group ◽  
Periodontal Diseases ◽  
Main Group ◽  
Control Group ◽  
Treatment And Prevention ◽  
Group A ◽  
Group B ◽  
Periodontal Indices

Goal. Improving the scheme of treatment and prevention of periodontal diseases in workers of the woodworking industry of Chernivtsi region, who in the process of professional activity have long-term contact with unfavorable factors of the production environment. Materials and methods. 70 workers of the woodworking industry with diagnosed chronic generalized periodontitis of the I degree were treated (35 people – the main group (A); 35 people – comparison group (B)), age – from 25 to 45 years. To compare the results of laboratory studies, an additional survey of 25 healthy individuals of the same age with an intact periodontium were conducted. They formed the control group (C). Clinical examination of patients was performed according to standart methods: subjective (complaints, medical history, life history) and objective (examination, periodontal indices, determination of the level of gingival attachment). Laboratory methods of research included determination of urease and lysozyme activity in saliva, degree of dysbiosis of oral cavity. As maintenance therapy, patients of the main group were prescribed the proposed composition DEPE. Results. After the treatment, a significant improvement in the hygienic status of patients in both groups was observed, but difference between groups A and B in the indicators of oral hygiene after treatment was no statistically significant (pA2–B2 > 0.05). There was a significant improvement in periodontal indices after treatment in patients of both groups (PMA index according to C. Parma, bleeding index according to Muhlemann in Cowell I. modification, Russell periodontal index, PSR-test), but the indicators in patients of the main group were significantly better – pA2–B2 < 0.05. In patients of the main group (A) the level of attachment loss decreased by 1.83 times, comparison group (B) – by 1.71 times. The difference in levels of attachment loss after treatment between groups A and B is statistically significant (pA2–B2 < 0.05). The activity of the enzyme urease in patients of the main group (A) and comparison group (B) after treatment was significantly reduced (pA1–A2 < 0.001, pB1–B2 < 0.001), but only in the main group it reached the level in the control group (C) (pA2–C > 0.05). The level of lysozyme after treatment was significantly increased in patients of both observation groups (pA1–A2 < 0.001, pB1–B2 < 0.001), and reached the level of lysozyme in patients of the control group (C) (pA2–C > 0.05, pB2–C > 0.05). The degree of oral dysbiosis in patients of the main group (A) was significantly reduced by 5.43 times (pA1–A2 < 0.001), and its difference from the degree of dysbiosis in patients of the control group (C) is statistically insignificant (pA2–C > 0.05 ). The degree of oral dysbiosis in patients of the comparison group (B) was significantly reduced by 3.04 times (pB1–B2 < 0.001), but still remained significantly different from oral dysbiosis in patients of the control group (C) (pB2–C < 0.05). Conclusion. The proposed pharmacological composition DEPE is an effective antiseptic solution and can be used in the phase of maintenance therapy to improve the treatment and prevention of periodontal disease in workers of woodworking industry. Key words: decamethoxine, propolis, ethonium, generalized periodontitis, woodworking industry.

scholarly journals Duration of Postoperative Hyperlactatemia Has Predictive Value in Recurrent Fistula After Major Definitive Surgery for Intestinal Fistula

2021 ◽  
Author(s):  
Weiliang Tian ◽  
Shikun Luo ◽  
Xin Xu ◽  
Zheng Yao ◽  
Risheng Zhao
Keyword(s):  
High Risk ◽  
Predictive Value ◽  
Cutoff Point ◽  
Intestinal Fistula ◽  
Recurrent Fistula ◽  
A Value ◽  
Group A ◽  
Definitive Surgery ◽  
Group B

Abstract Purpose The present study aimed to identify the predictive value of duration of postoperative hyperlactatemia in screening patients at high risk of recurrent fistula after major definitive surgery(DS) for intestinal fistula. Methods If the initial postoperative lactate(IPL)> 2 mmol/L, DS was defined as major definitive surgery. The 315 enrolled patients with major DS were divided into group A (2 mmol/L<IPL≤ 4 mmol/L), group B (mmol/L<IPL≤ 6 mmol/L), and group C (IPL>6 mmol/L). The characteristics of patients were collected, and the duration of postoperative hyperlactatemia was analyzed. According to the occurrence of recurrent fistula (RF), patients were further divided into RF group A, and Non-RF group A; RF group B, and Non-RF group B; and RF group C, and Non-RF group C. Results The duration of postoperative hyperlactatemia was comparable between the RF group A and the Non-RF group A [12 (IQR:12-24) vs 24 (IQR:12-24), p=0.387]. However, the duration of hyperlactatemia was associated with RF in group B (adjusted OR= 1.061; 95%CI: 1.029-1.094; p<0.001) and group C (adjusted OR=1.059; 95%CI: 1.012-1.129; p=0.017). In group B, the cutoff point of duration of 42 hours had the optimal predictive value (area under ROC=0.791, sensitivity=0.717, specificity =0.794, p<0.001). In group C, the cutoff point of duration of 54 hours had the optimal predictive value (area under ROC=0.781, sensitivity=0.730, specificity =0.804, p<0.001). Conclusion The duration of postoperative hyperlactatemia has a value in predicting RF in patients with an IPL of more than 4 mmol/L after major definitive surgery for intestinal fistula.

scholarly journals Effect of Sijunzi Pills on Pharmacokinetics of Omeprazole in Beagle Dogs by HPLC-UV: A Herb-Drug Interaction Study

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Hua-ling Xia ◽  
Xin-jie Zhao ◽  
Yu-ji Zhang ◽  
Xiao-hang Su ◽  
Bo Sun ◽  
...  
Keyword(s):  
Internal Standard ◽  
Hplc Method ◽  
Array Detector ◽  
Pharmacokinetic Parameters ◽  
Beagle Dogs ◽  
Beagle Dog ◽  
Dog Plasma ◽  
Group A ◽  
Group B

A sensitive high-performance liquid chromatography (HPLC-UV) method for determination of omeprazole in beagle dog plasma was developed and to investigate the effect of Sijunzi pills (SJZPs) on the pharmacokinetics of omeprazole in beagle dogs. The beagle dog plasma was extracted with ethyl acetate and n-hexane under alkaline conditions. Omeprazole and internal standard (IS, fluconazole) were separated on an XDB-C18 column, and acetonitrile and 0.1% trifluoroacetic acid were used as the mobile phase. Omeprazole and IS were detected by using a diode array detector. This experiment adopts the experimental design of double-cycle self-control. In the first cycle (group A), six beagle dogs were given omeprazole 0.67 mg/kg orally in a single dose. In the second period (group B), the same six beagle dogs were orally given SJZPs 0.2 g/kg twice a day for 7 consecutive days, and then, omeprazole was orally given. At the different time points after omeprazole was given in the two periods, the blood samples were collected. The concentration of omeprazole was detected by the developed HPLC method. DAS 2.0 was used to calculate the pharmacokinetic parameters of omeprazole. Under the current experimental conditions, this UPLC method showed good linearity in the detection of omeprazole. Interday and intraday precision did not exceed 10%, and the range of accuracy values were from −1.43% to 2.76%. The results of extraction recovery and stability met the requirements of FDA approval guidelines of bioanalytical method validation. The Cmax of omeprazole in group B was 61.55% higher than that in group A, and the AUC(0−t) and AUC(0−∞) of omeprazole in group B were 63.96% and 63.65% higher those that in group A, respectively. At the same time, the clearance (CL) and apparent volume of distribution (Vd) decreased in group B. In this study, an HPLC method for the determination of plasma omeprazole concentration was established. SJZPs could inhibit the metabolism of omeprazole and increase the concentration of omeprazole in beagle dogs. It is suggested that when SJZPs are combined with omeprazole, attention should be paid to the herb-drug interactions and possible adverse reactions.

scholarly journals Utilidad diagnóstica de la relación plaquetas/linfocitos en embarazadas con preeclampsia

2021 ◽  
Vol 81 (01) ◽  
pp. 5-12
Author(s):  
Eduardo Reyna-Villasmil ◽  
◽  
Jorly Mejía-Montilla ◽  
Nadia Reyna-Villasmil ◽  
Duly Torres-Cepeda ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Predictive Value ◽  
Control Group ◽  
Study Group ◽  
Diagnostic Efficacy ◽  
Lymphocyte Ratio ◽  
Platelet Lymphocyte Ratio ◽  
A Value ◽  
Group A ◽  
Group B

Objective: To establish the diagnostic utility of the platelet-lymphocyte ratio in pregnant women with preeclampsia. Methods: A case-control study was carried out at the Hospital Central “Dr. Urquinaona” Maracaibo, Venezuela. A total of 180 pregnant women were selected. Ninety preeclamptic women were included as the study group (group A) and a control group selected for their age and body mass index similar to the study group, which consisted of 90 healthy normotensive pregnant women (group B). The general characteristics, platelet-lymphocyte ratio values and diagnostic efficacy were determined. Results: Group A patients presented lower platelet and lymphocyte values compared to group B patients (p <0.001). However, no statistically significant differences were found in platelet-lymphocyte ratio between group A patients (115.2 ± 32.7) and group B patients (122.3 ± 23.8; p = 0, 0971). A cut-off value of the platelets-lymphocyte ratio of 117 presented a value below the curve of 0.57, the sensitivity of 47.8%, the specificity of 50.0%, the positive predictive value of 48.9%, and the negative predictive value of 52.2%, with a diagnostic accuracy of 48.9%. Conclusion: Platelet-lymphocyte ratio is not a useful tool in the diagnosis of preeclampsia, since patients with the syndrome present similar values to normotensive pregnant women. Keywords: Platelet-lymphocyte ratio, Preeclampsia, Diagnosis, Pregnancy.

scholarly journals Maternally-Derived Antibody and Seroconversion to Infectious Bronchitis Virus in Chicken

1970 ◽  
Vol 25 (1) ◽  
pp. 53-56 ◽  
Author(s):  
Narayan Chandra Paul ◽  
M Shahidur Rahman Khan ◽  
Shaiful Islam ◽  
AHM Taslima Akhter ◽  
Niraj Kanti Shil
Keyword(s):  
Antibody Titre ◽  
Infectious Bronchitis Virus ◽  
Serum Samples ◽  
Infectious Bronchitis ◽  
Field Virus ◽  
Group A ◽  
Enzymelinked Immunosorbent Assay ◽  
And Gender ◽  
Group B

An investigation was conducted with a view to determining the persistence of maternally-derived antibody (MDA) against infectious bronchitis virus (IBV) in chicks as well as seroconversion of IBV in relation to age and gender of chicks in field condition. Determination of antibody titre was performed by indirect enzymelinked immunosorbent assay (iELISA). The existence of MDA was calculated in Group A (n = 20) and Group B (n = 15) originating from parents vaccinated against IBV and with no such history respectively. In case of group A, titres of serum samples obtained were 5361.23 ± 854.09, 2567.58 ± 763.61, 808 ± 751.361, 432.29 ± 47.11, 178.36 ± 88.28, 184.58 ± 93.6, 80.89 ± 70.11 and 43.55 ± 32.92 on day 1, 5, 9, 13, 17, 21, 25 and 29 days aged birds respectively. On the other hand, sera samples of group B manifested a titre of 3285.18 ± 685.03, 2219.16 ± 419.77, 1783.62 ± 219.84, 589.01 ± 249.63, 186.66 ± 88.21, 178.36 ± 202.02, 105.77 ± 75.69 and 41.34 ± 69.28 on same schedule. It was further revealed that chicks with ancestor of non-vaccinated parents showed higher MDA titre up to 9th day of age. This might have happened due to exposure of field virus to the parent birds. As regards seroconversion, it was observed that 100, 20 and 80% of serum samples collected from chickens of 0-2 weeks, 3-4 weeks and 4 months of age were positive to IBV specific antibody respectively, whereas no serum sample was positive in case of 5-7 weeks aged birds. When considered the gender of birds, it was revealed that sera from the female showed 58.33% seropositivity against 25% from male. Maternally-derived antibody (MDA) might protect the chickens up to 9 days of age and since MDA could interfere vaccination, it should not be performed before such age. Keywords: Maternally-derived antibody (MDA); Infectious bronchitis virus (IBV); antibody titre; Seroconversion; ChicksDOI: http://dx.doi.org/10.3329/bjm.v25i1.4857 Bangladesh J Microbiol, Volume 25, Number 1, June 2008, pp 53-56

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