Molecular Typing of Multidrug Resistant Uropathogenic Escherichia coli by Restriction Fragment Length Polymorphism

2021 ◽  
Author(s):  
Soniya Goyal ◽  
◽  
Vikas Beniwal ◽  
Ravinder Kumar ◽  
Raman Kumar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Molecular Typing ◽  
Fragment Length Polymorphism ◽  
Multidrug Resistant ◽  
Uropathogenic Escherichia Coli ◽  
Restriction Fragment Length

Epidemiology of An Outbreak of Drug-Resistant Tuberculosis in the U.K. Using Restriction Fragment Length Polymorphism

1994 ◽  
Vol 86 (6) ◽  
pp. 749-751 ◽  
Author(s):  
M. Goyal ◽  
L. P. Ormerod ◽  
R. J. Shaw
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Multidrug Resistant ◽  
Drug Resistant ◽  
Resistant Disease ◽  
Resistant Tuberculosis ◽  
Restriction Fragment Length

1. Drug-resistant tuberculosis is a growing health care problem. When a series of cases occur, it is essential to know if patients with multidrug-resistant disease represent one or a number of separate outbreaks. 2. The epidemiology of an outbreak of isoniazid- and streptomycin-resistant tuberculosis in Blackburn was studied by restriction fragment length polymorphism using a probe for the IS6110 DNA sequence. 3. Mycobacterium tuberculosis from four cases of isoniazid- and streptomycin-resistant disease had an identical restriction fragment length polymorphism pattern. This pattern was not shared by drug-sensitive isolates of M. tuberculosis obtained from Blackburn (n = 8) or London (n = 13) or a M. tuberculosis isolate from a fifth Blackburn case which was resistant to isoniazid alone. 4. This methodology confirmed that all four cases of isoniazid- and streptomycin-resistant disease were part of a single epidemiologically related outbreak of drug-resistant disease. This study demonstrates how the epidemiology of an outbreak of multidrug-resistant tuberculosis in the U.K. can be confirmed by restriction fragment length polymorphism.

Human Lymphocyte Antigen Molecular Typing

2002 ◽  
Vol 126 (3) ◽  
pp. 281-284
Author(s):  
John A. Gerlach
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Human Lymphocyte ◽  
Fragment Length ◽  
Molecular Typing ◽  
Fragment Length Polymorphism ◽  
Hla Typing ◽  
Lymphocyte Antigen ◽  
Restriction Fragment Length

Abstract The human lymphocyte antigen (HLA) typing community was one of the early groups to adopt molecular testing. This action was borne out of the need to identify the many alleles of the highly polymorphic HLA system. Early paradigms used restriction fragment length polymorphism regimes, but the polymerase chain reaction method of amplification quickly replaced that less-than-discriminating choice. Methods currently in use for HLA typing, with commercial kits available, are sequence-specific oligonucleotide probe (both dot blot and the reverse blot dot), sequence-specific primer amplification, restriction fragment length polymorphism of amplified products, double-stranded sequence conformation polymorphism (with and without reference strand), sequence-based typing, and microarray technologies. More than 1250 alleles are recognized by the World Health Organization and meet their criteria for assignment. These alleles can be identified by molecular methods and represent alleles present at class I and class II loci of the HLA complex. On occasion, ambiguous results still persist, even with the best molecular typing methods. Therefore, it is clear to the HLA typing community that a combination of the above methods may be needed to allow true discrimination of the possible alleles an individual carries in their genetic makeup. It is also clear that a typing laboratory may need to resort to nonmolecular serology to understand the significance and impact of the type generated by the HLA molecular typing laboratory.

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