scholarly journals The Effect of Endurance Training Along with Curcumin on VEGF-A Level and VEGFR Gene Expression in Cancer Tissue of Female Mice with Breast Cancer

2020 ◽  
Vol 13 (2) ◽  
pp. 59-68
Author(s):  
فرامز کوچکی لنگرودی ◽  
مقصود پیری ◽  
محمد علی آذربایجانی ◽  
مریم دلفان ◽  
◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Endurance Training ◽  
Cancer Tissue ◽  
Female Mice

scholarly journals The BIRC Family Genes Expression in Patients with Triple Negative Breast Cancer

2021 ◽  
Vol 22 (4) ◽  
pp. 1820
Author(s):  
Anna Makuch-Kocka ◽  
Janusz Kocki ◽  
Anna Brzozowska ◽  
Jacek Bogucki ◽  
Przemysław Kołodziej ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Clinical Data ◽  
Triple Negative Breast Cancer ◽  
Triple Negative ◽  
Lymphatic Vessels ◽  
The Cancer Genome Atlas ◽  
Expression Level ◽  
Cancer Tissue ◽  
Expression Levels

The BIRC (baculoviral IAP repeat-containing; BIRC) family genes encode for Inhibitor of Apoptosis (IAP) proteins. The dysregulation of the expression levels of the genes in question in cancer tissue as compared to normal tissue suggests that the apoptosis process in cancer cells was disturbed, which may be associated with the development and chemoresistance of triple negative breast cancer (TNBC). In our study, we determined the expression level of eight genes from the BIRC family using the Real-Time PCR method in patients with TNBC and compared the obtained results with clinical data. Additionally, using bioinformatics tools (Ualcan and The Breast Cancer Gene-Expression Miner v4.5 (bc-GenExMiner v4.5)), we compared our data with the data in the Cancer Genome Atlas (TCGA) database. We observed diverse expression pattern among the studied genes in breast cancer tissue. Comparing the expression level of the studied genes with the clinical data, we found that in patients diagnosed with breast cancer under the age of 50, the expression levels of all studied genes were higher compared to patients diagnosed after the age of 50. We observed that in patients with invasion of neoplastic cells into lymphatic vessels and fat tissue, the expression levels of BIRC family genes were lower compared to patients in whom these features were not noted. Statistically significant differences in gene expression were also noted in patients classified into three groups depending on the basis of the Scarff-Bloom and Richardson (SBR) Grading System.

Comparison of the Results of PCR Analysis of Gene Expression in Breast Cancer Tissue Specimens Stabilized in Formalin and RNAlater

2014 ◽  
Vol 156 (4) ◽  
pp. 486-490 ◽  
Author(s):  
N. A. Khaustova ◽  
D. S. Makeeva ◽  
O. V. Kondrashina ◽  
N. N. Fedotov ◽  
I. N. Nechaev ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Breast Cancer Tissue ◽  
Pcr Analysis ◽  
Cancer Tissue ◽  
Tissue Specimens ◽  
Comparison Of The Results

Chronic moderate ethanol intake differentially regulates vitamin D hydroxylases gene expression in kidneys and xenografted breast cancer cells in female mice

2017 ◽  
Vol 173 ◽  
pp. 148-156 ◽  
Author(s):  
Janice García-Quiroz ◽  
Rocío García-Becerra ◽  
Galia Lara-Sotelo ◽  
Euclides Avila ◽  
Sofía López ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Vitamin D ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Ethanol Intake ◽  
Female Mice

scholarly journals ”BIK/NBK Gene Expression as a Possible Marker of Circulating Breast Cancer Cells in Blood“

2011 ◽  
Vol 4 (1) ◽  
pp. 8-14
Author(s):  
E. Lopez-Munoz ◽  
N. Garcia-Hernandez ◽  
R. I. Penaloza-Espinosa ◽  
M. E. Gomez-Del Toro ◽  
G. Zarco-Espinosa ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Cancer Cells ◽  
Therapeutic Target ◽  
Breast Cancer Cells ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Mrna Gene

The detection of circulating breast cancer cells in blood could be of special interest as an indicator of diagnosis and prognosis, and for the selection of treatment. In a previous report, our research group determined gene expression profiles in samples of breast cancer tissue, identifying over-expression of the BIK/NBK mRNA gene in 90% of the analyzed samples. In this paper, we analyze the BIK/NBK gene expression as a possible biomarker of circulating breast cancer cells in blood. We demonstrate that the BIK/NBK gene expression is not a significant biomarker in the detection of circulating breast cancer cells in the blood of women with breast cancer. Several studies have evaluated the regulation of apoptosis by estrogens in breast cancer cells, demonstrating the importance of BIK/NBK protein, in estrogen-regulated breast cancer cell apoptosis, which suggests that the regulation of its expression may be an important therapeutic target or strategy in the management of cancer, and, although we did not find statistically significant differences among the patient groups to demonstrate that BIK/NBK gene expression is a biomarker of circulating breast cancer cells in blood, we consider it necessary to continue the study of this gene in breast cancer tissue and its role in the development and progression of breast cancer, its prognostic value, and its potential use as therapeutic target.

scholarly journals Study of KMT2B (MLL2) gene expression changes in patients with breast cancer

2019 ◽  
Vol 8 (2) ◽  
pp. BMT24
Author(s):  
Mohammad Ghanbari ◽  
Mohammadali Hosseinpour-Feizi ◽  
Reza Safaralizadeh ◽  
Aida Aghazadeh ◽  
Vahid Montazeri
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Tumor Tissue ◽  
Rna Extraction ◽  
Cancer Tissue ◽  
Cdna Synthesis ◽  
Tissue Samples ◽  
Spss Software ◽  
Analyze Data ◽  
Mll2 Gene

Aim: This study aimed to demonstrate misregulation of KMT2B gene expression in breast cancer tissue. Materials & methods: Cancerous and marginal tissue samples were collected from 43 female patients. After RNA extraction and cDNA synthesis, quantitative-PCR was used to evaluate the expression level of the KMT2B gene. REST, Sigma plot and SPSS software were used to analyze data. Results: KMT2B gene expression was significantly decreased in tumor tissue compared with marginal tissue (p = 0.02). No significant correlation was found between expression levels of KMT2B and clinical parameters of patients (p > 0.05) Conclusion: Our study demonstrated that downregulation of KMT2B is associated with breast cancer and its misregulation may play an important role in tumorigenesis.

Quantitative nuclease protection assay (qNPA) for gene expression analysis on breast cancer core biopsies.

2011 ◽  
Vol 29 (27_suppl) ◽  
pp. 49-49 ◽  
Author(s):  
M. C. Evangelist ◽  
J. Snider ◽  
J. Krushkal ◽  
Y. Qu ◽  
A. Kulkarni ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Small Sample Size ◽  
Small Sample ◽  
Ffpe Tissue ◽  
Cancer Tissue ◽  
Breast Cancer Patients ◽  
Fresh Tissue ◽  
Interacting Protein ◽  
Oncogenic Pathways

49 Background: qNPA employs in situ hybridization of detection probes to cross linked mRNA, making it ideal for formalin fixed paraffin embedded (FFPE) tissue. It has been shown to measure gene expression in archived lymphoid and lung cancer tissue. We assessed the feasibility of qNPA to measure differentially expressed genes in pretreatment FFPE core breast biopsies among pathologic responders (pR) and nonresponders (pNR) to preoperative chemotherapy. Methods: We included preoperative breast cancer patients treated at our institution from 2003-09 with FFPE core biopsies. mRNA expression of 170 genes, representing oncogenic pathways or associated with anthracycline and taxane response was measured by qNPA (HTG, Tucson, AZ). Data was normalized to 3 housekeeper genes and average of 3 biologic replicates reported. Seven genes below detection in > 50% samples were excluded. Expression values of 163 unique genes were analyzed for pR vs pNR with dChip software. Empirical FDR was estimated using 1,000 permutations of sample labels. Results: Treatment and response: Sample failure: 6/57 (10%). pR vs pNR did not separate on hierarchical clustering. FLJ12650 and IGFBP2 showed lower expression in pR vs pNR with fold changes of 4.09 and 2.40, respectively (p < 0.01; median FDR: 1/163). FLJ12650 was significant (p < 0.01, median FDR: 0/163) when patients receiving anthracycline ± taxane were analyzed (groups 1-3) and showed a trend (p < 0.05) in group 1 alone. Conclusions: qNPA for limited available FFPE tissue from core biopsies is feasible with acceptable sample failure rates. Small sample size and number of analyzed genes limited definitive conclusion about informative genes in our study. Our FLJ12650 results, a gene coding for membrane Na+/K+ ATPase interacting protein, are consistent with previous findings of overexpression in pNR to anthracyclines + taxanes (Hess et al, JCO 2006, Vol 24; 4236) using fresh tissue. Future qNPA validation of predictive markers, identified by whole transcriptome analysis in a homogenous cohort may provide more definitive results. [Table: see text]

Sign in / Sign up

Export Citation Format

Share Document