Pengaruh Pemberian Ekstrak Anggur Laut terhadap Penurunan Kadar Kolesterol LDL Rattus norvegicus Jantan yang Mendapat Diet Tinggi Lemak

2020 ◽  
Vol 17 (2) ◽  
pp. 192
Author(s):  
RONALDO LAU ◽  
SULISTIANA PRABOWO ◽  
RIAMI RIAMI
Keyword(s):  
Cholesterol Level ◽  
Rattus Norvegicus ◽  
High Fat Diet ◽  
Ldl Cholesterol ◽  
White Male ◽  
Standard Diet ◽  
High Fat ◽  
Caulerpa Racemosa ◽  
Significant Difference ◽  
Male Wistar Rats

<p align="justify"><strong>ABSTRACT</strong><strong></strong></p><p align="justify"><strong>Background</strong>: High fat diet increase the absorption of lipid in the intestinum, that can lead to increase LDL cholesterol level in the blood. Sea grapes extract (<em>Caulerpa racemosa</em>) contains antioxidant polyphenolic group that can reduce MTP and ACAT-2 in the body that can decrease LDL cholesterol level in the blood.The purpose of this study is to know the effect of sea grapes extract  on decreasing LDL cholesterol of white male Wistar rats (<em>Rattus norvegicus</em>) fed with high fat diet.</p><p align="justify"><strong>Method</strong>:  24 white male Wistar rats, that divided into 3 groups: 1) group of rats fed with standard diet for 28 days; 2) group of rats fed with high fat diet for 28 days; 3) group of rats fed with high fat diet for 28 days and given 10 gram/kg body weight/day of sea grapes extract on 15<sup>th</sup>-28<sup>th</sup> days. Then the blood LDL cholesterol level measured on the 29<sup>th</sup> day.</p><p align="justify"><strong>Result :</strong> One-Way ANOVA Test showed there was significant difference (p=0.004) of LDL level between the group of rats fed with standard diet (12.37 mg/dl) compared to group of rats fed with high fat diet (17.87 mg/dl). There was significant difference (p=0.001) of LDL level between the group of rats fed with high fat diet (17.87 mg/dl) compared to group of rats fed with high fat diet and sea grapes extract (10.12 mg/dl).</p><p align="justify"><strong>Conclusion: </strong>high fat diet significantly increase blood LDL cholesterol level and sea grapes extract (<em>Caulerpa racemosa</em>) significantly decrease blood LDL cholesterol level.</p><p align="justify"> </p><p align="justify"><strong>Keywords :</strong>Sea grapes extract, LDL cholesterol, high fat diet</p>

scholarly journals PENGARUH PEMBERIAN CUKA APEL (Malus sylvestris Mill) VARIETAS MANALAGI TERHADAP KADAR TRIGLISERIDA DARAH TIKUS PUTIH (Rattus norvegicus) JANTAN GALUR WISTAR YANG DIBERI DIET TINGGI LEMAK DAN DIINDUKSI ALOKSAN

2019 ◽  
Vol 20 (2) ◽  
pp. 70-75
Author(s):  
Yuliyanti Yuliyanti ◽  
A. Sareh Arjono Tjandra ◽  
Herin Setianingsih
Keyword(s):  
Rattus Norvegicus ◽  
High Fat Diet ◽  
White Male ◽  
Male Rats ◽  
Control Group ◽  
Standard Diet ◽  
High Fat ◽  
Malus Sylvestris ◽  
Wistar Strain ◽  
Significant Difference

Hypertriglyceridemia is one of the risk factors for cardiovascular disease and acute pancreatitis (Pejic and Lee, 2006; Brahm and Hegele, 2013; Afari et al., 2015). One way to treat it by using the apple vinegar (Malus sylvestris Mill) of manalagi varieties which contained high antioxidants and acetic acid as well as more durable than the fresh fruit. The study aimed to know the effect of the Apple vinegar of manalagi varieties (Malus sylvestris Mill) to blood triglyceride levels of white male rats (Rattus norvegicus) wistar strain with high-fat diet and induced by alloxan. The study used the post test only control group design, with 24 rats samples which were divided into three groups. The negative control group (K0) only received standard diet for 24 days, the positive control group (K1) received a high-fat diet for 7 days and induced by alloxan with doses of 90 mg/kgBW of rats, and treatment group (K2) was given a high-fat diet for 7 days, induced by alloxan and received the apple vinegar (Malus sylvestris Mill) of manalagi varieties with a dose of 0,27 ml/100 g BW of rats for 14 days. The results by using one way ANOVA showed the significance value of the data p = 0,974 (p>0,05) so that there is no significant difference of the mean of blood triglycerides levels between K0, K1 and K2. This study concluded that apple vinegar (Malus sylvestris Mill) of manalagi varieties can not significantly lower the blood triglyceride levels of white male rats (Rattus norvegicus) wistar strain with high-fat diet and induced by alloxan. Further study about apple vinegar of manalagi varieties is required to define its effects.  Keywords—: High-Fat Diet, Alloxan, Triglyceride, Apple Vinegar (Malus sylvestris Mill) of Manalagi Varieties

scholarly journals PENGARUH AROMATERAPI MINYAK ATSIRI JAHE TERHADAP KADAR TRIGLISERIDA DAN KOLESTEROL DARAH TIKUS YANG DIBERI PAKAN TINGGI LEMAK

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Aji Agung Cahyaji
Keyword(s):  
Essential Oil ◽  
Total Cholesterol ◽  
Cholesterol Level ◽  
High Fat Diet ◽  
Ldl Cholesterol ◽  
Density Lipoprotein ◽  
Hdl Cholesterol ◽  
Albino Rats ◽  
Standard Diet ◽  
High Fat

The study aims to determine the effect of ginger (Zingiber officinale) essential oil via inhalation on blood triglyceride, total cholesterol, High Density Lipoprotein (HDL) cholesterol, and Low Density Lipoprotein (LDL) cholesterol level of rats that fed high fat diet. Eighteen albino rats (Rattus norvegicus) were devided into three treatments groups. The treatments were K1 (standard diet) as negative control, K2 (high fat diet) as positive control, and K3 (high fat diet + ginger essential oil inhalation). Blood samples were collected after 5 weeks of treatment period. The result showed the level of triglyceride, cholesterol, and HDL cholesterol at treatment K3 tend to be lower than treatment K2. LDL cholesterol level at treatment K3 show higher result than treatment K2. From the result of this study cocluded that inhalation of ginger essential oil can lowering triglyceride, total cholesterol, and LDL cholesterol level and raise HDL cholesterol level. Keywords: triglyceride, cholesterol, HDL cholesterol, LDL cholesterol, ginger essential oil

scholarly journals Efek jus belimbing pada kadar Total Kolesterol Rattus norvegicus yang diberi diet tinggi lemak

2019 ◽  
Vol 17 (1) ◽  
pp. 24
Author(s):  
PUTRI DIANA MAULIYAH ULFA ◽  
INDRI NGESTI RAHAYU ◽  
IGM ANTARA HAMBARSIKA
Keyword(s):  
Total Cholesterol ◽  
Cholesterol Level ◽  
Rattus Norvegicus ◽  
High Fat Diet ◽  
Total Cholesterol Level ◽  
Standard Diet ◽  
High Fat ◽  
Experimental Animals ◽  
Cholesterol Levels ◽  
Experimental Group

<p>Background: Hypercholesterolemia is a condition in which increased cholesterol concentration in the blood exceeds normal values. Consuming fats and foods rich in cholesterol can increase total cholesterol levels in the blood and cause cholesterol buildup in the arteries. Starfruit (Averrhoa bilimbi L.) has saponin and flavonoid compounds which are expected to play a role in reducing total cholesterol levels. The aim of this study is to determine the effect of star fruit juice (Averrhoa bilimbi L.) to total cholesterol blood levels of male Wistar strain (Rattus norvegicus) with a high-fat diet. Method: Experimental study with post-test only control group design. Twenty-four male white rats (Rattus norvegicus) of the Wistar strain are divided into 3 groups: the experimental group which is given standard diet for 28 days; group of experimental animals who were given a high-fat diet for 28 days; the experimental group treated with a high-fat diet for 28 days and on the 15<sup>th</sup>  until 28<sup>th</sup> days were given starfruit juice (Averrhoa bilimbi L.) with a dose of 4ml/200grwieght/day. On the 29th day, it is measuredtotal cholesterol levels for all groups of experimental animals. Results: The One-Way ANOVA test shows a significant increase in the average of total cholesterol levels (p = 0,009) in group of experimental animals that are given a high-fat diet (x = 60,625± 3,462mg/dL) compared to groups of experimental animals given standard diet (x = 50± 8,468mg/dL). There is a increase in the average in cholesterol level of the experimental group given a high-fat diet and the starfruit juice (Averrhoa bilimbi L.) (x = 65,5 ± 8,960) compared to the mean total cholesterol level of group of animals given a high-fat diet (x = 60,625± 3,462). However, there is no significant increase (p = 0,201). Conclusion: Administration of starfruit juice (Averrhoa bilimbi L.) was not significantly difference in statistics on average of cholesterol level to male wistar rat (Rattus norvegicus) induced by high fat diet</p><p>Keywords: high fat diet, starfruit juice, total cholesterol level</p>

scholarly journals The Comparison of Bay Leaf and Celery Leaf Infusion Effect on Decreasing LDL Level in Dyslipidemic Wistar Rats Model

1970 ◽  
Vol 1 (1) ◽  
pp. 21
Author(s):  
Anjani Putri Retnaninggalih ◽  
Erfan Efendi ◽  
Hairrudin Hairrudin
Keyword(s):  
Cholesterol Level ◽  
High Fat Diet ◽  
Wistar Rats ◽  
Ldl Cholesterol ◽  
Wistar Rat ◽  
Positive Control ◽  
Negative Control ◽  
High Fat ◽  
Leaf P ◽  
One Way Anova

Coronary heart disease (CHD) is the cause of 7,3 million death globally in 2008. The main pathophysiology process that leads to CHD is atherosclerosis which is caused by high LDL cholesterol level. The aim of this study was to explore bay leaf and celery leaf infusion effect on reducing LDL cholesterol level in dyslipidemic rat model. The type of this study was true experimental research using wistar rat (Rattus novergicus) as the sample. The rats were induced by high fat diet and PTU for two weeks then each of the group were given either bay leaf infusion, celery leaf infusion, simvastatin (positive control), or aquadest (negative control) for a week. The measurement of LDL level used Friedewald formulation. Data was analyzed using paired sample t test and one way ANOVA. As the result there was a significance reduction on LDL level after treatment with bay leaf (p=0,013) and celery leaf infusion (p=0,035) but there was no significance difference between groups (p=0,293). It was concluded that bay leaf and celery leaf infusion could reduce the LDL level in dyslipidemic wistar rat but there was no difference on the capability of the two different leafs on reducing LDL level. Keywords: dyslipidemia, LDL, bay leaf, celery leaf, wistar rats

Voluntary running of defined distances reduces body adiposity and its associated inflammation in C57BL/6 mice fed a high-fat diet

2017 ◽  
Vol 42 (11) ◽  
pp. 1179-1184 ◽  
Author(s):  
Lin Yan ◽  
Sneha Sundaram ◽  
Forrest H. Nielsen
Keyword(s):  
High Fat Diet ◽  
Plasma Concentrations ◽  
Standard Diet ◽  
Dependent Manner ◽  
High Fat ◽  
Body Adiposity ◽  
Chemotactic Protein ◽  
Voluntary Running ◽  
Significant Difference ◽  
Dose Dependent

This study investigated the effect of voluntary running of defined distances on body adiposity in male C57BL/6 mice fed a high-fat diet. Mice were assigned to 6 groups and fed a standard AIN93G diet (sedentary) or a modified high-fat AIN93G diet (sedentary; unrestricted running; or 75%, 50%, or 25% of unrestricted running) for 12 weeks. The average running distance was 8.3, 6.3, 4.2, and 2.1 km/day for the unrestricted, 75%, 50%, and 25% of unrestricted runners, respectively. Body adiposity was 46% higher in sedentary mice when fed the high-fat diet instead of the standard diet. Running decreased adiposity in mice fed the high-fat diet in a dose-dependent manner but with no significant difference between sedentary mice and those running 2.1 km/day. In sedentary mice, the high-fat instead of the standard diet increased insulin resistance, hepatic triacylglycerides, and adipose and plasma concentrations of leptin and monocyte chemotactic protein-1 (MCP-1). Running reduced these variables in a dose-dependent manner. Adipose adiponectin was lowest in sedentary mice fed the high-fat diet; running raised adiponectin in both adipose tissue and plasma. Running 8.3 and 6.3 km/day had the greatest, but similar, effects on the aforementioned variables. Running 2.1 km/day did not affect these variables except, when compared with sedentariness, it significantly decreased MCP-1. The findings showed that running 6.3 kg/day was optimal for reducing adiposity and associated inflammation that was increased in mice by feeding a high-fat diet. The findings suggest that voluntary running of defined distances may counteract the obesogenic effects of a high-fat diet.

scholarly journals Total Cholesterol Level of Hypercholesterolemia Male Wistar Rats (Rattus norvegicus) with Ethanol Extracts of Purple Sweet Potato Leaf (Ipomoea batatas (L.) Lam)

2018 ◽  
Vol 5 (1) ◽  
pp. 33
Author(s):  
Siti Fatimah ◽  
Desto Arisandi ◽  
Meirina Sindi Saputri
Keyword(s):  
Total Cholesterol ◽  
Cholesterol Level ◽  
Sweet Potato ◽  
Rattus Norvegicus ◽  
Total Cholesterol Level ◽  
High Fat ◽  
Cholesterol Levels ◽  
Male Wistar Rats ◽  
Purple Sweet Potato ◽  
Ethanol Extracts

The increase in total cholesterol level increases the risk of cardiovascular disease. Sweet potato leaves are a food that contains polyphenols, flavonoids, quercetin, tannin, and fiber that effectively lower total cholesterol levels. This study aims to analyze the effect of ethanol extracts of leaf purple sweet potato on serum total cholesterol levels of rats fed a high-fat diet. An experimental study with design pre and posttest carried out on 10 male Wistar rats (Rattus norvegicus) fed a high-fat, which are 2 groups: 1 group the control hypercholesterolemia and 1 group ethanol extracts of leaf purple sweet potato. Dose based on leaves of quercetin that is equal to 2 mg/kgBB. The average content of quercetin ethanol extracts of leaf purple sweet potato was analyzed to obtain 14,08 mg/100g. Ethanol extracts of leaf purple sweet potato are given for 14 days by way of on the sonde. Serum total cholesterol was determined by the CHOD-PAP method. The data obtained were analyzed descriptively.The result of this study obtained the average total cholesterol level of white blood rats before giving ethanol extracts of leaf purple sweet potato that is 189,89 mg/dl, whereas after administration of ethanol extracts of leaf purple sweet potato dose 2 mg/kgBB that is 131,92 mg/dl. The decrease in total cholesterol level of white rats after giving ethanol extracts of leaf purple sweet potato with dose 2 mg/kgBB that is 30,52 %.

scholarly journals PENGARUH PEMBERIAN JUS BUAH BELIMBING WULUH (AVERRHOA BILIMBI L.) TERHADAP KADAR KOLESTEROL LDL DARAH TIKUS PUTIH (RATTUS NORVEGICUS) JANTAN GALUR WISTAR DENGAN DIET TINGGI LEMAK

2019 ◽  
Vol 8 (2) ◽  
pp. 35-41
Author(s):  
Kartika Dwi Rahminiwati ◽  
◽  
IGM Antara Hambarsika ◽  
Fitri Handajani ◽  
◽  
...  
Keyword(s):  
Cholesterol Level ◽  
High Fat Diet ◽  
Ldl Cholesterol ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
High Fat ◽  
Cholesterol Levels ◽  
The Mean

A high-fat diet can increase lipoprotein levels, total cholesterol, (Low Density Lipoprotein) LDL, and triglycerides. Starfruit has saponin and flavonoid compounds which are expected to reducing LDL cholesterol levels. The aim of the study was to determine the effect of starfruit juice on lowering the blood cholesterol LDL of Wistar rats fed with high-fat diet. Experimental study with post-test only control group design. As many as 24 male white rats from the Wistar strain were divided into 3 groups: negative control groups (K-) that were given standard feed for 28 days; positive control group (K+) who were given a high-fat diet for 28 days; the treatment group (KP) was given a high-fat diet for 28 days and on the 15th day 28th they were given a starfruit juice with a dose of 4ml / 200grBB / day. Day 29 measured LDL cholesterol. The statistic test showed a significant increase in LDL cholesterol levels (p=0.001) in the positive control group (x=12.125±2.642 mg/dL) compared to the negative control group (x=7.625±1.506 mg/dL). There was no significant different the mean cholesterol level of the treatment group (x=11±1.927) compared to the mean LDL cholesterol level of the positive control group (x=12.125±2.642). A high-fat diet significantly increases the mean LDL cholesterol level. Starfruit juice did not significantly reduce LDL cholesterol level.

scholarly journals The Effectiveness of Purslane Extract Provision on Liver of White Rats (Rattus norvegicus) with High Fat Diet

2021 ◽  
Vol 11 (4) ◽  
pp. 608-613
Author(s):  
Yuli Susana ◽  
Lucia Tri Suwanti ◽  
Lianny Nangoi
Keyword(s):  
Rattus Norvegicus ◽  
High Fat Diet ◽  
Fatty Degeneration ◽  
The Body ◽  
Portulaca Oleracea ◽  
High Fat ◽  
Treatment Groups ◽  
White Rats ◽  
Significant Difference ◽  
The Mean

Most animal owners provide fatty food with high cholesterol level that exceeds the needs of their pets. The pattern of giving a high-fat diet causes interference with the cholesterol synthesis in the body. It can damage the liver by causing fatty degeneration, hepatic cirrhosis, and eventually necrosis of the liver. The purpose of the current study was to determine the effectiveness of Purslane extract (Portulaca oleracea L.) on the liver histopathological image of white rats (Rattus norvegicus) with a high-fat diet. The current study used an experimental method with a completely randomized design on 20 males white mouses (Rattus norvegicus) divided into five treatment groups. There were two control groups, the first group received standard feed (Pellet) and Carboxymethyl Cellulose Sodium 1%, while the second was given standard feed and a high-fat diet. Three treatment groups which included P1, P2, P3 were given standard feed, a high-fat diet, and purslane extract solution with 54 mg/day, 108 mg/day, and 216 mg/day, respectively. Research results from the mean rank of fatty degeneration which was measured using the Kruskal Wallis test presented significant differences, and the results from the mean of fatty degeneration using the Mann Whitney test did not indicate any significant difference. Liver cell necrosis from the two tests presented a significant difference. Purslane extract (Portulaca oleracea L.) was confirmed to be effective in reducing or decreasing hepatocyte cell damage after receiving a high-fat diet.

scholarly journals Pengaruh Pemberian Ekstrak Daun Mahkota Dewa pada Gambaran Histopatologi Paru Tikus Putih yang Dipapar Asap Rokok

2019 ◽  
Vol 17 (1) ◽  
pp. 48
Author(s):  
NGURAH BAGUS RADITYA SRI DAMAR ◽  
JANTO POERNOMO HADI ◽  
EDIJONO EDIJONO ◽  
TROEF SOEMARNO
Keyword(s):  
Inflammatory Response ◽  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Leaf Extract ◽  
White Male ◽  
Significant Difference ◽  
Lung Histopathology ◽  
Male Wistar Rats ◽  
Phaleria Macrocarpa

<p><strong>Background</strong>: Smoking is a bad habit for health. There are many dangerous components inside a cigarette. The smoke that comes out from the cigarette contains many harmful components. Smoking and getting exposed to cigarette smoke in a long period of time can cause inflammatory response on respiratory tract. Mahkota dewa leaf contains beneficial biological component, such as <em>flavonoid</em>, <em>alkaloid</em>, and<em>saponin </em>that have anti-inflammatory, anti-cancer, and many others.</p><p><strong>Aim :</strong>The purpose of this research was to determine the effect of administration of mahkota dewa leaf (<em>Phaleria macrocarpa</em>) extract onlung histopathology in white male Wistar rats (<em>Rattus norvegicus</em>) exposed to cigarette smoke.</p><p><strong>Methods : </strong>This research is a true laboratory experimental research using <em>Post-Test Only Control Group Design</em>. The subject in this research were 30 male Wistar Rats (<em>Rattus norvegicus</em>) which were divided into 4 groups, a group without given any treatment (K-), a group where they only getting exposed to cigarette smoke (K+), a group where they exposed to cigarette smoke and given mahkota dewa leaf extract at 1500 mg/kgBW/day dose (Eg1), and the group exposed to cigarette smoke and a mahkota dewa leaf extract at a 2500 mg/kgBW/day dose (Eg2).</p><p><strong>Result            : </strong>1.) There was a significant difference on lung histopathology between K- and K+, Eg1, and Eg2. 2.) There was no significant difference on lung histopathology between K+, Eg1 and Eg2.</p><p><strong>Conclusion : </strong>Exposure to 5 cigarette smoke in 21 days caused lung histopathology changes. However, there was no effect of the administration of mahkota dewa leaves extract at 1500 mg/kgBW/day and 2500 mg/kgBW/day on lung histopathology of white male Wistar rats exposed to 5 cigarette smoke in 21 days.</p><p><strong>Keyword : </strong><em>Phaleria macrocarpa leaf, </em>Flavonoid, Alkaloid, cigarette smoke, inflammatory response, Infiltration of Inflammatory cells.</p>

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