scholarly journals Uji Antagonisme Trichoderma harzianum Dan Azotobacter chroococcum Terhadap Rhyzoctonia solani, Sclerotium rolfsii dan Fusarium oxysporum secara in-vitro

Agrologia ◽  
2018 ◽  
Vol 7 (2) ◽  
Author(s):  
A. Marthin Kalay ◽  
Abraham Talahaturuson ◽  
Wilhemina Rumahlewang
Keyword(s):  
Trichoderma Harzianum ◽  
Sclerotium Rolfsii ◽  
Pathogenic Fungi ◽  
Azotobacter Chroococcum ◽  
Organic Media ◽  
Promising Alternative ◽  
Soil Borne Pathogens ◽  
Biological Fertilizer ◽  
In Vitro Treatment

Damage to plants by pathogenic fungi is often found in nurseries and field. Biological control offers a promising alternative for managing diseases in plants because they are environmentally friendly compared to pesticides. The aim of the study was to test the power of the antagonism of Trichoderma harzianum and Azotobacter chroococcum on solid organic media "TRIAZOTE"  which had been stored for five months against R. solani, S. rolfsii, and F. oxysporium pathogens. In-vitro treatment tested the power of antagonism of T. harzianum and A. chroococcum on growth of R. solani, S. rolfsii, and F. Oxysporum pathogens. The results showed that T. harzianum in "TRIAZOTE" which had been stored for five months could inhibit pathogenic growth R. solani, S. rolfsii, and F. oxysporum by 54.57%, 66.22%, and 68,57% respectively. While A. chroococcum did not significantly inhibit pathogenic growth of R. solani (11.02%), S. rolfsii (9.09%) and F. oxyprorum (10.99%). To control the disease in plants, especially those caused by soil borne pathogens R. solani, S. rolfsii and F. oxyprorum, can use biological fertilizer "TRIAZOTE" even though it has been stored for five months

scholarly journals Isolation of Endophytic Fungi from Vitex trifolia L and Antagonism Test against Sclerotium rolfsii and pathogenic bacteria

2021 ◽  
Vol 21 (1) ◽  
pp. 72
Author(s):  
Muhammad Hasan Basri ◽  
Lalu Zulkifli ◽  
Abdul Syukur
Keyword(s):  
Biological Control ◽  
Endophytic Fungi ◽  
Pathogenic Bacteria ◽  
Pathogenic Fungus ◽  
Sclerotium Rolfsii ◽  
Pathogenic Fungi ◽  
Promising Alternative ◽  
Environmental Friendly ◽  
Low Activity

Plant damage by pathogenic fungi is often found in plants, one of which is caused by Sclerotium rolfsii. Biological control strategy offers a promising alternative for managing disease in plants because they are environmental friendly compared to pesticides application. One of the biological control offered is by using endophytic fungi isolated from Vitex trivolia L. The aim of the study was to isolate, to identify macroscopic and microscopic endophytic fungi from Vitex trifolia L and to test their antagonism potency against the pathogenic fungus Sclerotium rolfsii in vitro. The isolation obtained 7 endophytic fungi isolates identified based on their genus characteristics, nsmely Periconia sp, Aspergillussp, Dendrophoma  sp, Geotrichum  sp, Ampulliferina  sp, Chalara  sp, dan Bispora sp and 2 isolates have not been identified. The Antibacterial test of the fungi isolate on the 4 tested bacteria showed that of all the fungi isolate have low activity. The antagonism test using the direct opposition method with the PIRG formula, showed that the 3 isolates had high percentage of growth inhibition, in which ALJ1, BLJ5, and ALJ3 isolate has  85%, 90%, and 100% respectively. This potency could be used as biological agents on the pathogenic fungus Sclerotium rolfsii.

scholarly journals Trichoderma harzianum como herramienta en el manejo integrado de enfermedades de tomate

2021 ◽  
Author(s):  
◽  
Román Müller
Keyword(s):  
Solanum Lycopersicum ◽  
Trichoderma Harzianum ◽  
Sclerotium Rolfsii ◽  
Trichoderma Spp ◽  
Solanum Lycopersicum L ◽  
Nuevas Tecnologías

En nuestro país como a nivel mundial, el tomate (Solanum lycopersicum L. = Lycopersicun esculetum Mill.), es una de las hortalizas más consumida y de mayor valor económico. Durante su cultivo, se encuentra expuesto a distintas plagas, entre ellas a Sclerotium rolfsii. Para un control eficiente de las enfermedades es conveniente utilizar un manejo integrado de plagas, utilizando distintas técnicas de cultivo tanto clásicas como nuevas tecnologías como el control biológico, que contemplen paralelamente el cuidado del medio ambiente y la producción del cultivo. Trichoderma spp., es uno de los organismos más utilizados para el control biológico de las enfermedades de origen fúngico. El objetivo de este trabajo fue evaluar la eficacia de la cepa de Trichoderma harzianum del IFSC como controlador biológico de Sclerotium rolfsii, ya sea sola o con fungicidas comerciales y analizar su capacidad como promotora de crecimiento del cultivo de tomate platense variedad Luna. Se evaluó el comportamiento de Th del IFSC para controlar a Sclerotium rolfsii in vitro, en cámara de crecimiento y a campo. Como resultado de los ensayos se comprobó que la cepa de Th del IFSC incrementó la germinación en condiciones in vitro e inhibió el desarrollo de S. rolfsii, poniéndose en evidencia el mecanismo de destrucción de los esclerocios. Indujo la promoción del crecimiento de la plántula obteniéndose plantas con mayor sanidad y porte. La cepa Th del IFSC tuvo mayor afinidad con el fungicida Captan incrementando la germinación y el desarrollo de las plántulas en el cultivo de tomate. Se comprobó la factibilidad del uso de Th del IFSC en un planteo de manejo integrado en el cultivo de tomate platense variedad Luna ya sea sola o con un fungicida como Captan.

scholarly journals Antagonismo de Trichoderma harzianum contra la fusariosis del garbanzo y su efecto biofertilizante

2020 ◽  
Vol 11 (5) ◽  
pp. 1135-1147
Author(s):  
Talina Olivia Martínez-Martínez ◽  
Brenda Zulema Guerrero-Aguilar ◽  
Víctor Pecina-Quintero ◽  
Enrique González-Pérez ◽  
Juan Gabriel Angeles-Núñez
Keyword(s):  
Rhizoctonia Solani ◽  
Fusarium Oxysporum ◽  
Trichoderma Harzianum ◽  
Fusarium Solani ◽  
Baja California ◽  
Sclerotium Rolfsii ◽  
Macrophomina Phaseolina

El garbanzo es una leguminosa, que se cultiva en dos regiones de México principalmente, noroeste (Sonora, Sinaloa y Baja california) y la región de El Bajío (Guanajuato, Michoacán y Jalisco); sin embargo, cada año la producción del cultivo está comprometida con la fusariosis vascular, una de las principales enfermedades que afectan al cultivo y que está asociada al complejo fúngico Fusarium oxysporum, Fusarium solani, Rhizoctonia solani, Macrophomina phaseolina y Sclerotium rolfsii. Una alternativa de control biológico es la aplicación de Trichoderma, la que además tiene un efecto indirecto en la nutrición de la planta. El objetivo de este estudio fue determinar el antagonismo in vitro de dos cepas de Trichoderma harzianum (T1 y T2) y su efecto como biofertilizante. Se realizaron confrontaciones in vitro contra cepas de las razas de Fusariumoxysporum f. sp. ciceris (Foc 0, 1B/C, 5 y 6), Fusarium solani, Macrophomina phaseolina (MSonora y M-GTO) y Sclerotium rolfsii. Se evaluó el efecto de T2 como biofertilizante (TB) midiendo las variables: número de flores, vainas, altura de la planta, diámetro del tallo, longitud de la raíz y rendimiento de grano. Las dos cepas de T. harzianum mostraron antagonismo en diferente escala contra los patógenos. Adicionalmente, con el tratamiento donde se aplicó T. harzianum (TB) se presentaron incrementos en el número de flores (30%), vainas (24%), altura (3%), diámetro de las plantas (3.5%), así como la longitud de la raíz (13%) y rendimiento del grano (23%).

scholarly journals The biological activity of subspecies Trichoderma harzianum against Fusarium oxysporum, the causative agent of fusarium wilt cucumber in vitro

2020 ◽  
Vol 21 ◽  
pp. 00021
Author(s):  
Ninel Pavlovskaya ◽  
Irina Gneusheva ◽  
Irina Solokhina ◽  
Natalya Ageeva
Keyword(s):  
Biological Activity ◽  
Fusarium Oxysporum ◽  
Trichoderma Harzianum ◽  
Pathogenic Fungi ◽  
Chitinase Activity ◽  
Lytic Enzymes ◽  
Growth Promoting ◽  
Phytopathogenic Microorganisms ◽  
Trichoderma Lixii

The study of the effect of the strains of the fungus Trichoderma: Trichoderma atrobrunneum VKPM F-1434, Trichoderma harzianum 5/14, Trichoderma Lixii T4/14 on the number of micromycetes populations of the pathogenic fungi Fusarium oxysporum isolate B/14, Fusarium oxysporum isolate MOS509, Fysarium oxysporum isolate IMI58289 in vitro. It was found that the Trichoderma atrobrunneum fungus strain VKPM F-1434 showed the highest degree of inhibition on 10 days of cultivation with phytopathogenic microorganisms, which was 100 %. The study revealed that all Trichoderma species are capable of producing lytic enzymes. Trichoderma atrobrunneum strain VKPM F-1434 exhibits strong lipase and chitinase activity and average proteinase activity. In addition, Trichoderma atrobrunneum strain VKPM F-1434 has a growth-promoting ability, which was reflected in the germination of seeds of cucumber “German F1”. The maximum values of indicators of germination energy were noted – 98.4 % and germination – 100 %.

Degradation of plant pathogenic fungi by Trichoderma harzianum

1982 ◽  
Vol 28 (7) ◽  
pp. 719-725 ◽  
Author(s):  
Y. Elad ◽  
I. Chet ◽  
Y. Henis
Keyword(s):  
Trichoderma Harzianum ◽  
Cell Walls ◽  
Sole Carbon Source ◽  
Hydrolytic Enzymes ◽  
Sclerotium Rolfsii ◽  
Pathogenic Fungi ◽  
Plant Pathogenic Fungi ◽  
Soilborne Pathogens ◽  
Glucanase Activity ◽  
Trichoderma Isolates

Trichoderma harzianum excreted β-1, 3-glucanase and chitinase into the medium when grown on laminarin and chitin, respectively, or on cell walls of the pathogen Sclerotium rolfsii, as sole carbon source. Trichoderma harzianum also showed high activity of both enzymes when grown on homogenized S. rolfsii sclerotia. Glucanase activity increased by 67% when the fungus was grown on a mixture of laminarin and glucose (3:1, v/v). Similarly, high lytic activity was detected in wheat bran culture of the fungus and in soil inoculated with this culture. Protease and lipase activity were detected in the medium when the antagonist attacked mycelium of S. rolfsii.Isolates of T. harzianum were found to differ in the levels of hydrolytic enzymes produced when mycelium of S. rolfsii, Rhizoctonia solani, and Pythium aphanidermatum in soil was attacked. This phenomenon was correlated with the ability of each of the Trichoderma isolates to control the respective soilborne pathogens.

scholarly journals KEMAMPUAN Trichoderma sp. DAN FILTRATNYA DALAM MENEKAN PERTUMBUHAN Sclerotium rolfsii SECARA IN VITRO

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Indah Puspita Dewi ◽  
Tri Maryono ◽  
Titik Nur Aeny ◽  
Suskandini Ratih
Keyword(s):  
Trichoderma Harzianum ◽  
Sclerotium Rolfsii ◽  
Trichoderma Sp

Salah satu penyebab rendahnya produksi kedelai nasional adalah adanya serangan penyebab penyakit rebah kecambah. Pengendalian yang dapat dilakukan adalah dengan menggunakan agen antagonis. Penelitian ini bertujuan mengetahui kemampuan Trichoderma sp. dan filtratnya dalam menekan pertumbuhan Sclerotium rolfsii secara in vitro. Pengujian terdiri atas uji antagonisme dengan metode kultur ganda dan uji filtrat Trichoderma sp. terhadap pertumbuhan S. rolfsii. Percobaan disusun dalam Rancangan Acak Lengkap (RAL) dengan lima ulangan. Data yang diperoleh dianalisis dengan sidik ragam dan dilanjutkan dengan uji BNT pada taraf nyata 5%. Hasil penelitian menunjukkan bahwa Trichoderma sp. dan filtratnya mampu menekan pertumbuhan S. rolfsii secara in vitro. Isolat Trichoderma sp. yang kemampuan penghambatannya paling tinggi adalah isolat Trichoderma harzianum.

scholarly journals Study on fungicidal effect of plant extracts on plant pathogenic fungi and the economy of extract preparation and efficacy in comparison to synthetic/chemical fungicides

2019 ◽  
Vol 11 (2) ◽  
pp. 333-337 ◽  
Author(s):  
Jyoti Singh ◽  
S. K. Bhatnagar ◽  
Akash Tomar
Keyword(s):  
Sclerotium Rolfsii ◽  
Pathogenic Fungi ◽  
Chemical Fertilizers ◽  
Herbal Products ◽  
Natural Origin ◽  
After Effects ◽  
Maximum Inhibition ◽  
Botanical Extracts ◽  
Chemical Fungicides

Providing food security to devastatingly increasing population with limited natural resources along with destruction caused by pre- and post-harvest pathogens are the foremost concerns for the developing countries. Numerous pesticides, herbicides and chemical fertilizers are being applied by the farmers to deal with the existing situation but leave very disastrous and undesirable after effects on ecosystem as non-degradable molecules.. Botanicals can be utilized as an ecofriendly and effective alternative against chemical as they are of natural origin. In this context, two chemical/synthetic fungicides namely Manzate and Nystanin in three different concentrations namely 500ppm, 1000 ppm and 1500 ppm were evaluated against Sclerotium rolfsii, Alternaria alternata, Fusarium monilifrome, Rhizoctonia solani and Aspergillus niger in vitro to compare them with ethanolic botanical extracts of spices (clove, cinnamon, thyme) and weeds (parthenium and calotropis) at 5%, 10%, 15%, 20% and 25%. Results revealed the high efficacy of botanicals i.e. clove extracts showed maximum inhibition (100%), followed by reduced inhibition in cinnamon, thyme, Parthenium and Calotropis treated plates against all five pathogens even at 5% concentration in comparison to chemical of 500 ppm concentration i.e. 100% in case of S.rolfsii only. Hence the herbal products can be further analyzed and applied as a potent, ecofriendly and economical substitute to chemicals.

scholarly journals Ability of Trichoderma harzianum in the Formulation with Carbon Fiber and Silica Nano Particles to Control Damping Off (Sclerotium rolfsii) on Soybean Plants

2018 ◽  
Vol 1 (2) ◽  
pp. 32-43
Author(s):  
Iman Ilahiyyat ◽  
◽  
Hersanti Hersanti ◽  
Luciana Djaya ◽  
Sri Hartati ◽  
...  
Keyword(s):  
Carbon Fiber ◽  
Trichoderma Harzianum ◽  
Sclerotium Rolfsii ◽  
Nano Particles ◽  
Second Phase ◽  
Damping Off ◽  
In Vivo Test ◽  
Soybean Plants

Sclerotium rolfsii is one of the soil-borne pathogens that cause damping-off and stem rot on soybean plants. One effort to control damping-off, which is environmentally friendly, is by using biological agents. Antagonistic microorganism that has been studied intensively and has a great potential to control soil-borne diseases is Trichoderma harzianum. The objectives of this research were to comprehend the ability of T. harzianum in a formulation with carbon fiber 80 mesh and silica nanoparticles (NPs.) and to determine the concentration of silica NPs. in the formulation that suppresses the in vitro growth of S. rolfsii and control the damping-off on soybean plants. The experiment was conducted in two phases. The first phase was in vitro experiment, arranged in a completely randomized design with 11 treatments and 3 replications. The second phase was in vivo test by using randomized complete block design, with 11 treatments and 3 replications. The in-vitro test showed that each treatment with T. harzianum in the formulation of silica NPs. and carbon fiber 80 mesh in various concentrations was able to suppress the S. rolfsii growth by 58.76- 80.92%. The treatment of single T. harzianum caused the highest suppression on S. rolfsii up to 80.92%. While the results of the in vivo test showed that the highest percentage of damping-off suppression was on the treatment of T. harzianum only, with 60% suppression.

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