scholarly journals Pertumbuhan In Vitro Tribulus terrestris dengan Perlakuan Indole Butyric Acid (IBA) dan Benzyl Amino Purine (BAP).

2021 ◽  
Vol 24 (1) ◽  
Keyword(s):  
Butyric Acid ◽  
Tribulus Terrestris ◽  
Indole Butyric Acid ◽  
Benzyl Amino Purine

Development of an embryo germination protocol for shy-seeded grape (Vitis vinifera L.)

2021 ◽  
pp. 1-9
Author(s):  
Ashwini P. Benke ◽  
Ram Krishna ◽  
Roshni R. Samarth ◽  
Shweta S. Dhumal ◽  
Waquar A. Ansari ◽  
...  
Keyword(s):  
Developmental Stages ◽  
Germination Rate ◽  
Germination Percentage ◽  
Vitis Vinifera L ◽  
Growth Media ◽  
Embryo Germination ◽  
Indole Butyric Acid ◽  
Grape Berries ◽  
Benzyl Amino Purine

Abstract Acquisition and germination of seeds are the most desired targets for the improvement of vegetatively propagated crops. In the present study, we developed a potential embryo germination protocol for the Red Globe grape cultivar having a low seed germination rate. Three grape berries at different developmental stages, viz. 50, 60 and 70 days after flowering (DAF), were selected for in-vitro embryo germination. Three growth media, namely Emershad and Ramming (ER), Nitsch and Nitsch (NN) and Murashige and Skoog (MS), and plant growth regulators (benzyl amino purine (BA), 0.5, 0.7 and 0.9 mg/l; indole butyric acid (IBA), 1.0, 1.5 and 2.0 mg/l; and gibberellic acid (GA), 0.1, 0.3 and 0.9 mg/l) were screened individually in different combinations with three amino acids, namely cysteine, glutamine and proline (2.0 μmol/l each). The maximum embryos germination percentage recorded at 70 DAF was 63.33, 47.78 and 45.56% in ER, NN and MS media, respectively, supplemented with 0.9 mg/l BA, 2.0 mg/l IBA, 0.9 mg/l GA and 2.0 μmol glutamine. Glutamine was found to have the most significant impact, and it improved the rescued embryos germination. The present study provides a potential recipe for a medium that can facilitate efficient germination of grape embryos.

scholarly journals In vitro Plantlets Regeneration from Nodal Segments of Murici (Byrsonima gardneriana)

2018 ◽  
Vol 10 (4) ◽  
pp. 402
Author(s):  
Francisca S. Sá ◽  
Jorge M. P. Porto ◽  
Alone L. Brito ◽  
José R. F. Santana ◽  
Rafaeli A. V. Souza ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Activated Charcoal ◽  
Indole Acetic Acid ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Indole Butyric Acid ◽  
In Vitro Plantlets ◽  
In Vitro Micropropagation

This study aimed to develop efficient protocols for the in vitro micropropagation of Byrsonima gardneriana. Nodal segments were obtained from seedlings germinated in vitro with 60 days of life. These were inoculated in MS/2 supplemented with 87.64 µM of sucrose and solidified with 0.7% of agar, supplemented with different concentrations of cytokinin 6-benzylaminopurine (0.0; 2.0; 4.0 and 8.0 µM) associated with different concentrations of auxin, indole acetic acid (0.0; 0.5 and 1.0 µM) and naphthaleneacetic acid (0.0; 0.5 and 1.0 µM). The sprouting were individualized and transferred to MS/2 cultures with different concentrations of indole butyric acid (0.0; 1.0; 2.0 and 3.0 µM), and presence and absence of activated charcoal (1.0 g L-1). The use of concentrations from 2.0 to 4.0 µM 6-benzylaminopurine was efficient in the multiplication of B. gardneriana, given that, using concentrations above these, a decrease in this efficiency occurs. The use of auxin interfered negatively with the results. In vitro rooting occurs even in medium free of auxin. The activated charcoal was insufficient for rooting. The use of growth regulators 6-benzylaminopurine and indole butyric acid are efficient in micropropagation of B. gardneriana, however, further studies should be performed to optimize this protocol.

scholarly journals Propagation Techniques for a Spineless Acacia wrightii

HortScience ◽  
2005 ◽  
Vol 40 (6) ◽  
pp. 1832-1837 ◽  
Author(s):  
Donita L. Bryan ◽  
Michael A. Arnold ◽  
R. Daniel Lineberger ◽  
W. Todd Watson
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Butyric Acid ◽  
Shoot Proliferation ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Root Number ◽  
Indole Butyric Acid ◽  
Tissue Culture Propagation

Three spineless phenotypes of Acacia wrightii G. Bentham ex A. Gray were identified with aesthetic landscape potential. Experiments in seed, cutting, grafting, and tissue culture propagation were undertaken to perpetuate this desired spineless phenotype. Germination percentages for mechanically scarified seeds ranged from 33% to 94%, however yield of spineless seedlings was low (0% to 34%). Sulfuric acid scarification for 10, 20, 30, or 60 minutes hastened and unified germination compared to nontreated seeds by 7 to 8 days. Vegetative propagation was successful for softwood cuttings. Rooting measures increased with auxin (2:1 indole butyric acid to naphthalene acetic acid) concentrations from 0 to 15000 mg·L–1, with maximum rooting percentage (70%), root number (9.2), and root length (12.4 cm) per softwood cutting at 15000 mg·L–1 auxin 8 weeks after treatment. Rooting was not successful for semi-hardwood or hardwood cuttings. Whip-and-tongue or T-bud grafting was not successful. Tissue culture of shoots from in vitro germinated seedlings indicated that shoot proliferation was greatest in Murashige and Skoog (MS) medium with 15 μm zeatin. The number of shoots that rooted in vitro increased with increasing concentrations of indole-3-butyric acid from 0 to 25 μm.

scholarly journals In Vitro Propagation of Pandoreas

HortScience ◽  
2001 ◽  
Vol 36 (2) ◽  
pp. 348-350 ◽  
Author(s):  
S. Latha Kancherla ◽  
Prem L. Bhalla
Keyword(s):  
Tissue Culture ◽  
Butyric Acid ◽  
In Vitro Propagation ◽  
Shoot Formation ◽  
Nodal Segments ◽  
Shoot Induction ◽  
Indole Butyric Acid ◽  
Commercial Cultivars ◽  
Murashige And Skoog Medium

Pandoreas, Australian natives of horticultural significance, were successfully propagated using tissue culture. A protocol for rapid in vitro multiplication of commercial cultivars was developed using nodal segments cultured on Murashige and Skoog medium containing either BA or kinetin. Maximum shoot induction and number of shoots per explant for P. pandorana (Andrews) Steenis and P. jasminoides (Lindley) Schumann were on 8.8 μm BA and 4.6 μm kinetin. Higher levels of cytokinin in the medium inhibited shoot formation. Tissue-cultured shoots were rooted with IBA. This study demonstrates that Pandoreas can be successfully micropropagated. Chemical names used: 6-benzylaminopurine (BA); 3-indole butyric acid (IBA).

scholarly journals Pertumbuhan Tanaman Nanas pada Berbagai Konsentrasi IBA Secara In Vitro

2014 ◽  
Vol 4 (2) ◽  
pp. 287-292
Author(s):  
Zarmiyeni Zarmiyeni ◽  
Siti Munawarah Munawarah
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Butyric Acid ◽  
Market Demand ◽  
Indole Butyric Acid ◽  
The People ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
A New Technique

       The people of Hulu Sungai Utara Regency are very fond of pineapple, this can be seen from the continued increase in market demand for pineapple. Pineapple production in Hulu Sungai Utara Regency from 2010-2013 continues to increase. However, the availability of pineapple is still widely supplied from other regions. This is due to limited land and the availability of superior seeds. Therefore we need a new technique to overcome this problem by multiplying it in vitro. This study aims (i) to determine the response of pineapple plant growth to various concentrations of IBA (Indole Butyric Acid) in vitro and (ii) to obtain the best IBA (Indole Butyric Acid) concentration for pineapple plant growth in vitro. The study was conducted in the Amuntai STIPER tissue culture laboratory from March to June 2014, using a Completely Randomized Design (CRD) with 5 treatment levels, 4 replications. The factor tested was the IBA concentration, i1 = 1.5 ppm, i2 = 2 ppm, i3 = 2.5 ppm, i4 = 3 ppm, and i5 = 3.5 ppm. The results showed no known response and the best concentration of IBA on pineapple plant growth.

scholarly journals Uji Komposisi Zat Pengatur Tumbuh Terhadap Pertumbuhan Eksplan Pisang Barangan (Musa paradisiaca L.) Pada Media MS Secara in vitro

2020 ◽  
Vol 3 (1) ◽  
pp. 101-111
Author(s):  
Rahmad Setia Budi
Keyword(s):  
Butyric Acid ◽  
Naphthaleneacetic Acid ◽  
Indole Butyric Acid ◽  
Musa Paradisiaca

Perbanyakan konvensional pisang secara umum melalui anakan (sucker) dan belahan bonggol (corm), namun bibit yang dihasilkan dengan cara ini menghasilkan sedikit anakan dan membutuhkan waktu relatif lama,  pertumbuhan tidak seragam sehingga penanaman skala besar akan sulit dilakukan. Perbanyakan melalui kultur jaringan pada pisang barangan telah dilakukan, hingga diperoleh bibit bermutu baik (seragam dan bebas patogen) dalam jumlah lebih banyak dan cepat. Keberhasilan menginisiasi pembentukan kalus dan tunas merupakan langkah awal untuk menghasilkan planlet dengan multiplikasi yang tinggi. Penelitian ini dilaksanakan di laboratorium kultur jaringan  UPT. Balai Benih Induk Gedung Johor Dinas Pertanian Provinsi Sumatera Utara. Tujuan penelitian untuk mengetahui pengaruh beberapa konsentrasi ZPT: (1). NAA (Naphthaleneacetic acid) dan BAP (Benzylamino purin) terhadap pertumbuhan eksplan pisang barangan secara kultur in vitro. (2). BA (Benzyl Adenin)  dan IBA (Indole Butyric Acid). Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) Faktorial. Hasil penelitian menunjukkan bahwa pengaruh konsentrasi NAA dan BAP; BA dan IBA terhadap pertumbuhan dan perkembangan eksplan tampak terutama dimulai pada minggu ke-2 setelah aplikasi hingga minggu ke-12. Konsentrasi BAP dan kombinasi konsentrasi NAA dan BAP serta konsentrasi BA dn IBA dan interaksinya tidak berpengaruh nyata terhadap persentase tumbuh, jumlah tunas, panjang tunas, dan bobot tunas

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