West Nile Virus Detection in Mosquitoes in East Baton Rouge Parish, Louisiana, from November 2002 to October 2004

2008 ◽  
Vol 24 (1) ◽  
pp. 28-35 ◽  
Author(s):  
Andrew J. Mackay ◽  
Alma Roy ◽  
Matt M. Yates ◽  
Lane D. Foil
Keyword(s):  
West Nile Virus ◽  
Baton Rouge ◽  
Virus Detection ◽  
West Nile ◽  
East Baton Rouge Parish

Evaluation of Surveillance Methods for Detection of West Nile Virus Activity in East Baton Rouge Parish, Louisiana, 2004–2006

2009 ◽  
Vol 25 (2) ◽  
pp. 126-133 ◽  
Author(s):  
Isik Unlu ◽  
Alma F. Roy ◽  
Matt Yates ◽  
Daniel Garrett ◽  
Heather Bell ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Baton Rouge ◽  
West Nile ◽  
East Baton Rouge Parish ◽  
Virus Activity

WEST NILE VIRUS SURVEILLANCE IN EAST BATON ROUGE PARISH, LOUISIANA

2007 ◽  
Vol 23 (1) ◽  
pp. 29-36 ◽  
Author(s):  
RAQUEL M. GLEISER ◽  
ANDREW J. MACKAY ◽  
ALMA ROY ◽  
MATHEW M. YATES ◽  
RANDY H. VAETH ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Baton Rouge ◽  
West Nile ◽  
East Baton Rouge Parish ◽  
Virus Surveillance

scholarly journals First record of West Nile Virus detection inside wild mosquitoes in Khartoum capital of Sudan using PCR

2020 ◽  
Vol 27 (12) ◽  
pp. 3359-3364
Author(s):  
Rania Ali El Hadi Mohamed ◽  
Deena M. Abdelgadir ◽  
Hind M. Bashab ◽  
Laila A. Al-Shuraym ◽  
Fadilah Sfouq Aleanizy ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Virus Detection ◽  
First Record ◽  
West Nile

Establishment and evaluation of real-time PCR for West Nile virus detection

2009 ◽  
Vol 6 (1) ◽  
pp. 55-59 ◽  
Author(s):  
Shi Li-Jun ◽  
Lu Mao-Min ◽  
Li Gang ◽  
Li Cheng-Yao ◽  
Zhang Jin-Gang
Keyword(s):  
West Nile Virus ◽  
Real Time ◽  
Virus Detection ◽  
Rt Pcr ◽  
Pcr Assay ◽  
West Nile ◽  
Capsid Protein Gene ◽  
Specific Test ◽  
Intercalating Dye ◽  
Polymerase Chain

AbstractA rapid real-time polymerase chain reaction (RT-PCR) for detecting West Nile virus (WNV) was established. Primers were designed according to the sequence of the capsid protein gene of WNV by Primer Premier 5.0. In this way, an inexpensive assay using the intercalating dye SYBR Green I was developed and validated. The amplifying curve showed that this method could successfully amplify 102 copies/μl of the WNV gene, while reference to Japanese encephalitis virus (JEV) and blank control were all negative. Tenfold successive dilutions of positive WNV DNA were used to measure the sensitivity of RT-PCR. The assay system showed high reproducibility with coefficient of variation (CV) <2%. Thus the newly established RT-PCR assay was shown to be a rapid, sensitive and specific test for detecting WNV.

scholarly journals West Nile Virus Detection and Commercial Assays

2005 ◽  
Vol 11 (7) ◽  
pp. 1154-1155 ◽  
Author(s):  
Peter A.G. Tilley ◽  
Gail A. Zachary ◽  
Roberta Walle ◽  
Paul F. Schnee
Keyword(s):  
West Nile Virus ◽  
Virus Detection ◽  
West Nile

scholarly journals Virus Detection Protocols for West Nile Virus in Vertebrate and Mosquito Specimens

2003 ◽  
Vol 41 (8) ◽  
pp. 3661-3667 ◽  
Author(s):  
E. B. Kauffman ◽  
S. A. Jones ◽  
A. P. Dupuis ◽  
K. A. Ngo ◽  
K. A. Bernard ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Virus Detection ◽  
West Nile

scholarly journals Development of a Double Antibody Sandwich ELISA for West Nile Virus Detection Using Monoclonal Antibodies against Non-Structural Protein 1

PLoS ONE ◽  
2014 ◽  
Vol 9 (10) ◽  
pp. e108623 ◽  
Author(s):  
Xi-Xia Ding ◽  
Xiao-Feng Li ◽  
Yong-Qiang Deng ◽  
Yong-Hui Guo ◽  
Wei Hao ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
West Nile Virus ◽  
Sandwich Elisa ◽  
Virus Detection ◽  
Structural Protein ◽  
West Nile ◽  
Double Antibody

scholarly journals WEST NILE VIRUS DETECTION PROGRAMME, SURVEILLANCE OF Culex sp. IN MONTENEGRO, 2019

2019 ◽  
Vol 65 (4) ◽  
Author(s):  
Igor PAJOVIC ◽  
Miladin RALEVIC ◽  
Bojan ADZIC ◽  
Ljiljana PAJOVIC
Keyword(s):  
West Nile Virus ◽  
Virus Detection ◽  
West Nile ◽  
Culex Sp ◽  
Detection Programme

scholarly journals First serological evidence of West Nile virus among equines and birds in Kosovo, 2018-2019

2020 ◽  
Author(s):  
Agim Rexhepi ◽  
Kurtesh Sherifi ◽  
Kristaq Berxholi ◽  
Betim Xhekaj ◽  
Nesade Muja-Bajraktari ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Neutralization Test ◽  
Virus Detection ◽  
Control Program ◽  
Serum Samples ◽  
West Nile ◽  
Health Authorities ◽  
Domestic Bird ◽  
Domestic Birds ◽  
Public Health Authorities

Abstract Background: This study was conducted to survey the presence of the West Nile virus (WNV) in Kosovo by serological testing of the healthy autochthonous equine population and virus detection in birds and mosquitoes. Between January 2018 and June 2019, 260 equine serum samples, 626 mosquitoes (60 pools), 50 domestic birds and 51 wild birds were collected from different regions of Kosovo. Equine and domestic bird serum samples were tested by IgG ELISA while mosquitoes and bird viscera were tested for WNV RNA by RT-PCR. Positive ELISA samples were confirmed by Plaque Reduction Neutralization Test (PRNT) and eight by Virus Neutralization Test (VNT). Results: This is the first report providing evidence of WNV antibodies among animals in Kosovo. WNV antibodies were present in 27 out of 260 equine sera (10.38%) and one out of 50 samples in domestic birds by ELISA and PRNT. Eight of 27 positive equine serum samples were confirmed by VNT. No WNV RNA was detected in birds or mosquitoes.Conclusions: The occurrence of WNV antibodies in autochthonous equines from all regions of Kosovo indicates that the virus is circulating within the country. Public health authorities should therefore plan a risk assessment and disease control program.

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