scholarly journals Transmitted Light as Attractant with Mechanical Traps for Collecting Nocturnal Mosquitoes in Urban Bangkok, Thailand

2021 ◽  
Vol 37 (3) ◽  
pp. 132-142
Author(s):  
Manop Saeung ◽  
Pairpailin Jhaiaun ◽  
Michael J. Bangs ◽  
Ratchadawan Ngoen-Klan ◽  
Theeraphap Chareonviriyaphap

ABSTRACT Mosquito surveillance is the cornerstone for determining abundance, species diversity, pathogen infection rates, and temporal and spatial distribution of different life stages in an area. Various methods are available for assessing adult mosquito populations, including mechanical trap devices using different forms of attractant cues (chemical and visual) to lure mosquitoes to the trap. So-called “light traps” use various electromagnetic wavelengths to produce a variety of visible spectral colors to attract adult mosquitoes. However, this type of trapping technology has not been widely used in Thailand. This study compared the efficacy of 4 light-emitting diodes (LEDs) (blue, green, yellow, and red) and 2 fluorescent (ultraviolet [UV] and white) lights for collecting mosquitoes in urban Bangkok. Using a Latin square experimental design, 6 light traps equipped with different lights were rotated between 6 trap site locations within the Kasetsart University (KU) campus. Each location received 6 replicate collections (6 consecutive trap-nights represented 1 replicate) over 36 collection nights for a total of 216 trap-nights. Traps were operated simultaneously (1800 to 0600 h), with captured mosquitoes removed at 3-h intervals. In total, 2,387 mosquitoes consisting of 11 species across 5 genera (Aedes, Anopheles, Armigeres, Culex, and Mansonia) were captured. Collectively, Culex species represented the predominant group sampled (2,252; 94.4%). The UV light source captured 1,544 (64.7%) of the total mosquitoes collected, followed by white 389 (16.3%), with the 4 LED sources collecting between 6.8% (blue) and 1.9% (yellow). Traps equipped with UV light were clearly the most effective for capturing nocturnally active mosquito species on the KU campus.

2021 ◽  
Vol 67 (1) ◽  
pp. 69-72
Author(s):  
Nicholas Acevedo

The CDC light trap has been the standard used by mosquito control programs to conduct mosquito and arbovirus surveillance. For the last two decades, this trap has been used with little to no modifications to its original design. Recently, new traps that utilize different light sources, modified designs, and attractants have been developed and evaluated against the CDC light trap. A semi-field and field comparison of the Dynatrap® (Model DT2000) against the CDC light trap was conducted at Anastasia Mosquito Control District. The DT2000 varies from the CDC light trap with a UV light, trapdoor/fan mechanism, and Atrakta lure which is a combination of lactic acid, ammonia, and hexanoic acid. Overall, the DT2000 collected 56% (327/600) of the Ae. aegypti released in the semifield cage, compared to 18.5% (111/600) collected by the CDC light traps. These findings suggest that the DT2000 outperformed the CDC light trap in collecting Ae. aegypti. In the field, the DT2000 collected nine target mosquito species while the CDC light trap collected four target species. The DT2000 averaged 109 ± 97.46 mosquitoes and the CDC light trap averaged 8 ± 4.64 mosquitoes. The DT2000 presented functional limitations in the field as an electrical outlet was required. Study findings suggest that where an electrical outlet is available, the DT2000 may be an alternative to the CDC light trap for mosquito surveillance.


Author(s):  
Timothy D McNamara ◽  
Thomas A O’Shea-Wheller ◽  
Nicholas DeLisi ◽  
Emily Dugas ◽  
Kevin A Caillouet ◽  
...  

Abstract West Nile virus (WNV) is the most prevalent arbovirus found throughout the United States. Surveillance of surface breeding Culex vectors involved in WNV transmission is primarily conducted using CDC Gravid traps. However, anecdotal claims from mosquito abatement districts in Louisiana assert that other trap types may be more suited to WNV surveillance. To test the validity of these assertions, we conducted a series of trapping trials and WNV surveillance over 3 yr to compare the efficacy of multiple trap types. First, we compared the CDC Gravid trap, CO2-baited New Standard Miniature Blacklight traps, and CO2-baited CDC light traps with either an incandescent light, a red light, or no light. We found that the CDC Gravid trap and CO2-baited no-light CDC Light trap collected the most mosquitoes. Second, we conducted additional, long-term trapping and WNV surveillance to compare these two trap types. We found that CO2-baited no-light CDC traps collected more of the local WNV vector, Culex quinquefasciatus (Say, Diptera, Culicidae), and detected WNV with greater sensitivity. Finally, we conducted trapping to compare the physiological states of Cx. quinquefasciatus and diversity of collected mosquitoes. CO2-baited no-light CDC light traps collected more unfed Cx. quinquefasciatus while Gravid traps collected more blooded Cx. quinquefasciatus; both traps collected the same number of gravid Cx. quinquefasciatus. Additionally, we found that CO2-baited no-light CDC light traps collected a larger diversity of mosquito species than Gravid traps.


Author(s):  
Rebecca A Zimler ◽  
Donald A Yee ◽  
Barry W Alto

Abstract Recurrence of local transmission of Zika virus in Puerto Rico is a major public health risk to the United States, where mosquitoes Aedes aegypti (Linnaeus) and Aedes mediovittatus (Coquillett) are abundant. To determine the extent to which Ae. mediovittatus are capable of transmitting Zika virus and the influence of viremia, we evaluated infection and transmission in Ae. mediovittatus and Ae. aegypti from Puerto Rico using serial dilutions of infectious blood. Higher doses of infectious blood resulted in greater infection rates in both mosquitoes. Aedes aegypti females were up to twice as susceptible to infection than Ae. mediovittatus, indicating a more effective midgut infection barrier in the latter mosquito species. Aedes aegypti exhibited higher disseminated infection (40–95%) than Ae. mediovittatus (<5%), suggesting a substantial midgut escape barrier in Ae. mediovittatus. For Ae. aegypti, transmission rates were low over a range of doses of Zika virus ingested, suggesting substantial salivary gland barriers.


Insects ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 248
Author(s):  
Marina E. O. Rangel ◽  
Luana P. R. Oliveira ◽  
Aline D. Cabral ◽  
Katharyna C. Gois ◽  
Marcos V. M. Lima ◽  
...  

In 2018–2019, we conducted mosquito collections in a municipal vehicle impound yard, which is 10 km from the Serra do Mar Environmental Protection Area in Santo André, SP, Brazil. Our aim is to study arboviruses in the impound yard, to understand the transmission of arboviruses in an urban environment in Brazil. We captured the mosquitoes using human-landing catches and processed them for arbovirus detection by conventional and quantitative RT-PCR assays. We captured two mosquito species, Aedes aegypti (73 total specimens; 18 females and 55 males) and Ae. albopictus (34 specimens; 27 females and 7 males). The minimum infection rate for DENV-2 was 11.5 per 1000 (CI95%: 1–33.9). The detection of DENV-2 RNA in an Ae. albopictus female suggests that this virus might occur in high infection rates in the sampled mosquito population and is endemic in the urban areas of Santo André. In addition, Guadeloupe mosquito virus RNA was detected in an Ae. aegypti female. To our knowledge, this was the first detection of the Guadeloupe mosquito virus in Brazil.


2021 ◽  
Vol 118 (23) ◽  
pp. 231102
Author(s):  
Youn Joon Sung ◽  
Dong-Woo Kim ◽  
Geun Young Yeom ◽  
Kyu Sang Kim

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